Figure 1
From: Identification of Schistosoma mansonigender-associated gene transcripts by cDNA microarray profiling
High-quality total RNA was isolated from adult male and female schistosomes and reverse transcribed into cDNA which hybridized with equal affinity to control S. mansoni genomic elements. (a) Total RNA was isolated from 7-week-old male (M) and female (F) parasites by iterative phase separation and affinity chromatography techniques. A 10 μg sample of each RNA preparation was electrophoresed under denaturing conditions on a 0.1% agarose gel [58]. Intense 18S rRNA bands are visible with minimal apparent sample degradation. (b) Analysis of mean fluorescent signal intensities (MFI) detected from all arrayed S. mansoni genomic elements after hybridization with cDNA generated from 10 μg male and female RNA samples. Three representative hybridization experiments (out of five) are shown, which show no statistically significant difference in the mean fluorescent intensity (MFI) between male and female cDNA samples, and support the contention that equivalent quantities of both sample materials were used in each experimental hybridization. Standard errors of the MFI are indicated.