Figure 3
Pairwise comparisons of prothoracic, mesothoracic and metathoracic leg discs (leg1, leg2 and leg3, respectively). Cluster analysis was carried out on the dataset with the requirement to show induction >1.74 (0.8 of the log2-transformed R/G ratio). In the color representation of the cluster results in this and the subsequent figures, the columns represent the different experiments and the rows indicate the genes. Seventeen genes were found to be differentially expressed between the first (ten genes, green cluster) and second leg discs (seven genes, red cluster) in three independently repeated experiments (numbers 91, 77 and 142). The comparison of first and third leg discs (two experiments, numbers 92 and 94) produced two genes in the leg1 cluster (green). Two experiments (numbers 106 and 81) revealed 12 genes that are differentially expressed between leg2 disc (three genes, green cluster) and leg3 disc (nine genes, red cluster). Note the expression of Antp in the second leg disc in the leg1-to-leg2 and leg2-to-leg3 comparisons, with more than threefold induction in both cases (see text). The columns indicate the subclusters with consistent induction in one channel, the gene identification numbers (ID), the average fold induction of the two or three comparisons (Fold), the gene name and function as published on Flybase [44]. The color code is indicated below with the numbers representing the fold induction.