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Table 1 Internal control genes evaluated in this study

From: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Symbol Accession number Name Function Localization Pseudo gene* Primers Alias IMAGE
ACTB NM_001101 Beta actin Cytoskeletal structural protein 7p15-p12 + S   510455
B2M NM_004048 Beta-2-microglobulin Beta-chain of major histocompatibility complex class I molecules 15q21-q22 - S   51940
GAPD NM_002046 Glyceraldehyde-3-phosphate dehydrogenase Oxidoreductase in glycolysis and gluconeogenesis 12p13 + D   510510
HMBS NM_000190 Hydroxymethyl-bilane synthase Heme synthesis, porphyrin metabolism 11q23 - D Porphobilinogen deaminase 245564
HPRT1 NM_000194 Hypoxanthine phosphoribosyl-transferase 1 Purine synthesis in salvage pathway Xq26 + D   345845
RPL13A NM_012423 Ribosomal protein L13a Structural component of the large 60S ribosomal subunit 19q13 + D 23 kDa highly basic protein -
SDHA NM_004168 Succinate dehydrogenase complex, subunit A Electron transporter in the TCA cycle and respiratory chain 5p15 + D   375812
TBP NM_003194 TATA box binding protein General RNA polymerase II transcription factor 6q27 - D   280735
UBC M26880 Ubiquitin C Protein degradation 12q24 - D   510582
YWHAZ NM_003406 Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, zeta polypeptide Signal transduction by binding to phosphorylated serine residues on a variety of signaling molecules 2p25 + S§ Phospholipase A2 416026
  1. *Presence (+) or absence (-) of a retropseudogene in the genome determined by BLAST analysis of the mRNA sequence using the high-throughput genomic sequences database (htgs) or human genome as database. Localization of forward and reverse primer in different exons (D) or the same exon (S). IMAGE cDNA clone number according to [14]. § A single-exon gene.