ORMDL proteins are a conserved new family of endoplasmic reticulum membrane proteins

Table 2 Summary of experimental and in silico data on the ORMDL gene family members

Organism	Gene name	cDNA (bp)	Protein (amino acids)	Annotation (from RefSeq, GenBank, TAIR, FlyBase, SGD, YPD, PombePD)	Chromosomal location	Structural domains^*	Expression pattern^†	Subcellular localization^†
H. sapiens	ORMDL1	1,092^†	153	LOC51240 [39]	2q32.2	4 TM	Ubiquitous in adult and fetal tissues	Endoplasmic reticulum
	ORMDL2	934^†	153	HSPC160[39]	12q13.2	3 TM	Ubiquitous in adult and fetal tissues	Endoplasmic reticulum
	ORMDL3	869^†	153	None	17q21.1	4 TM	Ubiquitous in adult and fetal tissues	Endoplasmic reticulum
	ORMDL1	(pseudogene)	None	10p14	-	-	-
	ORMDL2	(pseudogene)	None	8q22.1	-	-	-
M. musculus	ORMDL1	1,815	153	None	1^‡	4 TM	NT	NT
	ORMDL2	1,053	153	0610012C09 gene [12]	10^‡	4 TM	NT	NT
	ORMDL3	2,024	153	2810011N17 gene [12]	11^‡	4 TM	NT	NT
T. rubripes	ORMDL1	-	153	None		4 TM	NT	NT
	ORMDL2	-	153	None		4 TM	NT	NT
	ORMDL3	-	153	None		4 TM	NT	NT
C. intestinalis	ORMDL	-	153	None		4 TM	NT	NT
D. melanogaster	ORMDL	388^†, partial cds	154	CG14577 [3]	3L 78E6	3 TM	Ubiquitous at early embryonic stages and in ectodermal derived tissues at later stages	NT
A. thaliana	ORMDLa	795	157	F6F3.4; At1g01230^§	1	2 TM	NT	NT
	ORMDLb	none	154	MJC20.10; At5g42000^¶	5	4 TM	NT	NT
S. cerevisiae	ORM1 ^¥	none	222	ORM1 [40]; G4089; YGR038w	VII	4 TM	NT	NT
	ORM2 ^#	none	216	YLR350w [41]; L8300.1	XII	4 TM	NT	NT
S. monacensis	ORM1	None	222	ORM1 [40]		4 TM	NT	NT
S. pombe	ORM	1167, partial cds	186	SPBC119.09c [42]	2	3 TM	NT	NT
E. cuniculi	ORMDL	-	147	ECU11_1150 [43]	XI	4 TM	NT	NT

^*Potential transmembrane segments were predicted using the HMMTOP 2.0 program [26]. ^†Experimental data obtained in the present study. ^‡Mapping of mouse ORMDL genes was inferred from syntenic regions between human and mouse chromosomes. ^§ GenBank accession no. AF360237. ^¶GenBank accession no. NC_003076. ^¥The null mutant constructed in the present study is viable; the double null mutant with ORM2 is viable but shows an impaired growth rate^†; the ORM1 mRNA is more abundant in MATα cells than in MAT a cells, and at 39°C than at 30°C [44]; ORM1 is coregulated with other 117 genes under 26 cell-damaging conditions [45] and is induced during sporulation [46]; Orm1 protein is produced in mid-log cells [47]. ^#The null mutant constructed in the present study is viable; the double null mutant with YGR038w (ORM1) is viable but shows an impaired growth rate^†; Orm2 interacts with Slt2p in a systematic two-hybrid assay [21]; ORM2 is coregulated with other 121 genes under 26 cell-damaging conditions [45] and is induced by a 30 min and 90 min treatment with 1 M NaCl [48]. TM, transmembrane segments; NT, not tested.

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