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Genome Biology volume 3, Article number: reports0031 (2002)
A study of carotenoid synthesis was carried out to discover inhibitors that might provide new herbicides
Significance and context
Carotenoids - carotenes and xanthophylls - give color to fruits and flowers, and have an important role in photosynthesis, where they participate in light harvesting and protect the photosynthetic apparatus against high light intensities. Carotenoid synthesis involves a phytoene synthase (PSY), a phytoene desaturase (PDS), a ζ-carotene desaturase (ZDS), and lycopene cyclases to form, for instance, β- and α-carotene, zeaxanthine, violaxanthin, and neoxanthin. The study of carotenoid synthesis in plants is interesting from a nutritional point of view to increase the vitamin A content, and also to discover ways of blocking carotenoid synthesis and thus removing their protective role in photosynthesis. To find appropriate targets for such herbicidal action, Busch et al. focused on the effects on carotenoid synthesis of up- and down-regulating the expression of the genes psy and pds from Nicotiana tabacum (tobacco).
Using PCR and primers designed against conserved regions within reported similar gene sequences, two copies of psy and one copy of pds were isolated from tobacco. The corresponding proteins show about 90% or more identity to those of described PSY and PDS proteins, respectively. The coding sequences of psy1, psy2, and pds were expressed in sense and antisense in transgenic tobacco plants, obtained by transformation mediated by Agrobacterium tumefaciens, and the effects on metabolism and phenotype were studied. Some plants overexpressing psy1 showed severe phenotypes, including dwarfism, changes in leaf pigmentation, a rolled appearance of the leaves, young leaves that appeared orange-colored, buds that were unable to flower, and flower-color changes. Overexpression of psy2 led to similar but less severe phenotypes in a number of plants. In plantsoverexpressing either psy1 or psy2, changes in carotenoid and chlorophyll content were noticed. In orange capsules that surround the seeds, for instance, the carotenoid content increased up to 400% and the chlorophyll content decreased to 6% as compared to green capsules. Antisense psy1 and psy2 expression did not show any significant phenotypes in primary transformants. Overexpression of pds did not cause any phenotypic or metabolic effects, in contrast to plants expressing pds antisense in which a dramatic accumulation of phytoene was noticed together with an increased carotenoid content. Some of the latter plants showed small white spots on their leaves, and white veins and leaf tips were noticed. Antisense expression of psy and pds was lethal for a population of transgenic plants.
The study was performed at Bayer AG and more background information concerning the company can be found at the Bayer AG website.
Busch et al. describe the effects of sense and antisense expression of two genes involved in the synthesis of carotenoids on tobacco phenotype and metabolism. Clearly, both the PSY and PDS enzymes can be used as targets to develop new herbicides, as the correct production of carotenoids seems to be important for tobacco survival. Purification and further characterization of these tobacco enzymes, and the isolation of similar genes from other plants, should be part of future research to reach this goal. The in-depth characterization of these enzymes in a variety of plant genera may also reveal unique features of these enzymes depending on the plant species, which may subsequently be exploited to develop more specific herbicides.
Busch M, Seuter A, Hain R: Functional analysis of the early steps of carotenoid biosynthesis in tobacco. Plant Physiol. 2002, 128: 439-453. 0032-0889
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D'Haeze, W. Inhibiting carotenoids . Genome Biol 3, reports0031 (2002). https://doi.org/10.1186/gb-2002-3-6-reports0031