Microarray profile of differentially expressed genes in a monkey model of allergic asthma

Table 2 Validation of microarray expression levels by RT-PCR in multiple 4-h challenged monkeys

		Single monkey^*		Multiple monkeys^†
Gene	Cluster	Array	RT-PCR	Fold ± SE	p-value^‡
TIMP-1	A	4.10	8.50	3.44 ± 1.74	0.0600
Thrombospondin	B	3.90	6.70	7.60 ± 1.40	0.0008
MCP-3	A	3.70	92.00	400 ± 244	0.0001
Mn superoxide dismutase	A	3.70	2.70	2.70 ± 0.06	0.0010
Pulmonary and activation-regulated chemokines	A	3.70	3.50	2.26 ± 0.60	0.0900
α₁-Antichymotrypsin	A	3.50	5.60	4.20 ± 1.20	0.0118
Eotaxin	A	3.00	15.00	13.40 ± 7.60	0.0264
ICAM-1	B	2.80	5.40	3.50 ± 0.63	0.0123
Plasminogen activator inhibitor-1	A	2.80	2.30	5.04 ± 1.06	0.0024
MCP-1	A	2.70	3.80	13.60 ± 4.00	0.0009
SM22	A	2.20	2.30	1.40 ± 0.38	0.3700
Chitotriosidase	C	0.77	0.67	1.10 ± 0.58	0.7700
Fibronectin	E	0.59	0.50	0.61 ± 0.04	0.6400
Calcitonin gene-related peptide receptor-1	E	0.40	0.50	1.08 ± 0.34	0.6500
Integrin alpha 6	D	0.38	0.50	0.64 ± 0.05	0.3700
N-acetylgalactosaminyl-transferase-2	D	0.37	1.90	1.26 ± 0.27	0.5000
Selenoprotein-P	D	0.36	0.20	0.55 ± 0.16	0.1300
Orphan nuclear hormone receptor BD73	D	0.23	0.27	0.29 ± 0.12	0.0279
Pepsinogen C	D	0.20	0.31	0.50 ± 0.30	0.0427
DMBT1	D	0.20	0.22	0.26 ± 0.04	0.1900

^*Fold expression level determined by microarray or RT-PCR in original 4-h challenged monkey. ^†Mean fold expression (± SE) determined by RT-PCR in 4-h challenged monkeys (n = 4) compared to normal, control monkey (n = 6). ^‡p-values were determined by t-test (unpaired).

ISSN: 1474-760X