Figure 6

Structural characteristics of DNA insertion sites. Sites for (a) roo; (b) pogo; (c) jockey. Genomic sequence flanking the insertion site of each element was extracted from our dataset. Those elements for which duplicated target sequences could not be identified were discarded. The remaining sequences from each family were centered on the repeat (vertical gray line) and the average denaturation temperature across all sequences was determined using a 3-bp window size. In each panel, the light horizontal gray line represents the average denaturation temperature of random genomic sequence and the horizontal black line represents the average denaturation temperature of the experimental set of sequences. The x-axis represents the distance (in bp) from the insertion site and the y-axis represents the temperature (°C). The sequences flanking the roo (a) and pogo (b) elements have opposite characteristics; the roo sequences have a higher than average denaturation temperature whereas the pogo sequences have a lower than average denaturation temperature. The average denaturation temperature of the sequence flanking the jockey elements does not differ from that of the random sequence.