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Table 3 The presence of chorismate mutase and aromatic aminotransferase in the periplasmic fraction of Pseudomonas aeruginosa

From: The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa

Fractions

Enzymes

 

Total enzyme activities

   

PA01

PAT1051

Periplasmic fraction

DT

Total PDT

1154

1154

  

*PheC§ (marker)

1154

1154

  

PheA

0

0

  

*AroQ

184

81

  

AAT

181

244

  

SDH (marker)

13

38

Cytoplasmic fraction

DT

Total PDT

1373

71

  

*PheC§ (marker)

50

60

  

PheA

1323

11

  

AroQ•

605

22

  

AAT

862

2528

  

SDH (marker)

375

478

Whole-cell extract

DT

Total PDT

2706

617

  

*PheC§

1220

604

  

PheA

1486

13

  

*AroQ + AroQ•

734

88

  

AAT

905

3714

  

SDH

402

512

  1. Total enzyme activities are expressed as units (nmol of product permin) per 150 ml of culture. Prephenate dehydratase activity assayed in the absence of L-phenylalanine. §Prephenate dehydratase activity assayed in the presence of 1 mM L-phenylalanine. Calculated as 'Total PDT' minus '*PheC'. DT,dehydratase; PDT, prephenate dehydratase; *PheC, cyclohexadienyl dehydratase; PheA, prephenate dehydratase domain of AroQPheA; AroQ, chorismate mutase domain of aroQPheA; *AroQ, periplasmic chorismate mutase; AAT, aromatic aminotransferase; SDH, shikimate dehydrogenase.