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Table 3 The presence of chorismate mutase and aromatic aminotransferase in the periplasmic fraction of Pseudomonas aeruginosa

From: The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa

Fractions Enzymes   Total enzyme activities
    PA01 PAT1051
Periplasmic fraction DT Total PDT 1154 1154
   *PheC§ (marker) 1154 1154
   PheA 0 0
   *AroQ 184 81
   AAT 181 244
   SDH (marker) 13 38
Cytoplasmic fraction DT Total PDT 1373 71
   *PheC§ (marker) 50 60
   PheA 1323 11
   AroQ• 605 22
   AAT 862 2528
   SDH (marker) 375 478
Whole-cell extract DT Total PDT 2706 617
   *PheC§ 1220 604
   PheA 1486 13
   *AroQ + AroQ• 734 88
   AAT 905 3714
   SDH 402 512
  1. Total enzyme activities are expressed as units (nmol of product permin) per 150 ml of culture. Prephenate dehydratase activity assayed in the absence of L-phenylalanine. §Prephenate dehydratase activity assayed in the presence of 1 mM L-phenylalanine. Calculated as 'Total PDT' minus '*PheC'. DT,dehydratase; PDT, prephenate dehydratase; *PheC, cyclohexadienyl dehydratase; PheA, prephenate dehydratase domain of AroQPheA; AroQ, chorismate mutase domain of aroQPheA; *AroQ, periplasmic chorismate mutase; AAT, aromatic aminotransferase; SDH, shikimate dehydrogenase.