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Table 2 Genome-wide assessment of ab initio gene prediction methods

From: A draft annotation and overview of the human genome

Genscan

Grail

Fgenes

Transcript-

Protein-

Unconfirmed

   

confirmed

supported

exons

   

exons

exons

 

•

  

25,619

2,890

45,025

 

•

 

52,644

14,685

434,409

  

•

7,791

796

257,676

•

•

 

17,841

3,761

28,556

  

•

13,915

1,711

11,628

 

•

•

3,990

450

49,420

•

•

•

53,566

9,871

20,569

Total exons

  

175,366

34,164

847,283

  1. Three gene prediction programs, Genscan [93], Fgenes [94] and Grail1.3 [95] were used to screen individual genomic contigs. Exons consistently predicted by more than one program are merged into a unique exon index, which is then compared to transcript- and protein-based exons in complete ORFs. Transcript-confirmed exons, overlapping of predicted exons with transcript-based exons; protein-supported exons, predicted exons have at least strong protein homology (E < 10-15); unconfirmed exons, predicted exons have no overlap with transcripts nor protein homology.