Figure 5From: Gene trapping identifies transiently induced survival genes during programmed cell deathImproved survival of FDCP1 cells following apoptotic prestimulation. (a) Colony formation in soft agar. One thousand FDCP1 cells were seeded into agar cultures as previously described [9]. After incubation for 0, 1, 2 and 4 h, IL-3 was added at the indicated concentrations. Colonies were counted in duplicate after incubating for 7 days. Results were derived from two independent experiments. (b) Frequency of apoptotic cells. 1 × 106 cells were deprived of IL-3 for 1 and 2 h, respectively. After re-adding IL-3 for 4 h, cells were washed and incubated without IL-3. After 6, 9 and 12 h factor deprivation, the cells were stained with annexin and the frequency of apoptotic cells was estimated by flow cytometry. Results were derived from two independent experiments.Back to article page