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Table 2 Comparison of fold change between oligonucleotide arrays and RT-PCR

From: Identification of candidate downstream genes for the homeodomain transcription factor Labial in Drosophila through oligonucleotide-array transcript imaging

  Avg Diff (array) Fold change
Gene hs-wt hs-lab Array RT-PCR
lab 41 1078 26.3 55.7
swa 20 406 20.3 18.4
UbcD4 44 423 9.6 6.5
twe 20 132 6.6 4.9
cycB 243 1344 5.5 4.6
Uch 61 312 5.1 12.1
sqd 373 370 1.0 1.1
scrt 225 79 -2.9 -3.7
Pepck 610 171 -3.6 -4.6
  1. RT-PCR was performed on cDNA derived from heat-shocked wild-type embryos and heat-shocked hs-lab embryos. Fold changes determined by RT-PCR are represented as the mean values of eight independent replicates, derived from two different cDNA preparations. Avg Diff, absolute average difference value (see Materials and methods).