Heat-shock-driven ubiquitous overexpression of lab monitored by in situ hybridization and immunocytochemistry. (a-d) RNA in situ hybridization; (e-h) immunocytochemical staining. Expression of lab is shown in heat-shocked wild-type embryos (a,c,e,g) and in heat-shocked embryos carrying a hs-lab construct (b,d,f,h). (a,b,e,f) Overview of stage 10-17 embryos. (c,d) Higher magnification of a single stage 15 embryo and (g,h) a single stage 13 embryo; lateral view, and anterior to the left. Embryos were exposed to a heat shock at 36°C for 25 min and were allowed to recover for another 25 min before fixation.