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Table 1 BLAST programs

From: Having a BLAST with bioinformatics (and avoiding BLASTphemy)

Program

Query sequence type

Target sequence type

 

BLASTP

Protein

Protein

Compares an amino acid query sequence against a protein sequence database

BLASTN

Nucleotide

Nucleotide

Compares a nucleotide query sequence against a nucleotide sequence database

BLASTX

Nucleotide (translated)

Protein

Compares a nucleotide query sequence translated in all reading frames against a protein sequence database

TBLASTN

Protein

Nucleotide (translated)

Compares a protein query sequence against a nucleotide sequence database dynamically translated in all reading frames

TBLASTX

Nucleotide (translated)

Nucleotide (translated)

Compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database