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Table 1 Induction methods for RNAi by feeding

From: Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans

  Non-Ind Ind (1) Ind (2) Ind (3) Ind (4)
Test gene n % Phe n % Phe n % Phe n % Phe n % Phe
gpb-1 546 0 530 100 309 84 442 97 346 0
unc-22 422 0 255 99 179 80 ND ND ND ND
par-1 ND ND 313 100 263 100 ND ND ND ND
par-3 ND ND 391 96 325 11 ND ND ND ND
  1. Four different methods were compared to determine optimal induction conditions for RNAi; non-induced (Non-Ind) bacteria were also included for comparison. Induction conditions (Ind) were as follows: (1) Bacteria were induced on plates with IPTG at room temperature overnight; (2) bacteria were induced in culture at 37°C for 2 h; (3) bacteria were induced on plates with IPTG at 37°C overnight; (4) bacteria were induced in culture at 37°C overnight (see the Materials and methods section for detailed protocols). gpb-1, par-1 and par-3 were scored for percentage of dead embryos, unc-22 was scored for percentage of worms with an uncoordinated phenotype. Data shown represent the progeny of three fed worms. ND, not done; n is the number of worms or embryos scored; %Phe, percentage of worms or embryos with phenotype.