Table 3 Oligonucleotide primers used to amplify and sequence mouse and human Tlr4 genes
From: Phylogenetic variation and polymorphism at the Toll-like receptor 4 locus (TLR4)
Mouse | Â | Primate |
|---|---|---|
Amplification | Â | Â |
Exon 1 | ↑ CAGTCGGTCAGCAAACGCCTTCTTC | ↑GCTCGGTAAACGGTGATAG |
|  | ↓CAAGGCAGGCTAGCAGGAAAGGGTG | ↓TGAGAAGTTCTGGGCAGAAG |
Exon 2 | ↑ TTATTCATCTTTGGAGAGGAGTGG | ↑TCTCTGGTCTAGGAGAGG |
|  | ↓ AAGGAAGTTTAGTTAGAACCACCTTG | ↓ CCAGTCCAATAATGAAATG |
Exon 3 | ↑ TCTCCTGCTCACACCATCATCACCTG | ↑ CCAGTCCAATAATGAAATG |
|  | ↓ CATGTGTTCCATGGGCTCTCGGTC | ↓ TGACTTTCTTTGTCATGGGTTCCTTGACTG |
Sequencing | Â | Â |
Exon 1 same as above | Â | Â |
Exon 2 same as above | Â | Â |
Exon 3 primary | 1↓ ATGCCATGCCTTGTCTTC | 3.1 ↑ GAGCTGGATGACTAGGATTAATATTC |
|  | 2↓TTTAAATTCTCCCAAG | 3.1 ↓TCAAATTGCACAGGCCCTCTAG |
|  | 3↓CAGCTCTTCTAGACC | 3.2 ↑CAATCTCTCTTTAGACCTGTCC |
|  | 4↑ TGTGAACATCAGAAATTCCT | 3.2 ↓AATACTTTAGGCTGGTTGTCCC |
|  | 5↑ TGAGATTGCTCAAACATGG | 3.3 ↑ GAAGTTGATCTACCAAGCCTTG |
|  | 6↑ TTGAAACAATTGAAGACAAGGC | 3.3 ↓ GGAAGTCATTATGTGATTGAGAC |
|  | 7↑ CCTGGCTGGTTTACACGTC | 3.4↑ CTTCCTGGACCTCTCTCAGTGTCAAC |
|  | 8↑ TTTCATGGGTCTAGAAGAGCTG | 3.4↓ GAAGGCAGAGCTGAAATGGAGG |
|  | 9↓ AAGAACTGCTTCTGTTCC | 3.5↑ TCAGATGAATAAGACCATCATTGGTG |
|  | 10↓ TCAGAAACTGCCATGTTTG | 3.5↓ AACAAGTGTTGGACCCAG |
Exon 3 secondary | 5'↑ TGAGCTGGTAAAGAATTTAG | 1↑ GTAAATTTGGACAGTTTCC |
|  | 7'↑ CTGACGAACCTAGTACATGTG | 2↑ TTCAGTATTCCTATCACTCAG |
|  | 9'↓ ATGTCAAGTTTGTTGTGTT | 3↑ TTATAAGTGTCTGAACTCCC |
|  |  | 4↑ TCGGTCCTCAGTGTGCTTG |
|  |  | 5↑ GTGTCCCAGCACTTCATC |
|  |  | 6↑ AACCTCCTGAGGCATTTC |
|  |  | 7↓ GTTTCAAATTGGAATGCTG |
|  |  | 8↓ AAGGAAACGTATCCAATG |
|  |  | 9↓ AAGCACACTGAGGACCGAC |
|  |  | 10↓ GATGAAGTGCTGGGACAC |
|  |  | 11↓ TCCTCTCTAGATAGATGTTG |
|  |  | 12↓ TTTCTTTGTCATGGGTTC |
|  |  | 0↑ TTTAGGTTCTTATTCAGCAG |
|  |  | 0↓ GCTCTAGATTGGTCAGATTAG |