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  • Web report
  • Open Access

Chick pre-B cell encyclopedia

Genome Biology20001:reports227

https://doi.org/10.1186/gb-2000-1-1-reports227

  • Received: 9 December 1999
  • Published:

Abstract

For those interested in DT40, a chick pre-B-cell line proving increasingly popular for use in gene targeting projects, this is a very useful site.

Keywords

  • Cell Culture
  • cDNA Library
  • Reference List
  • Sequence Query
  • Chromosome Preparation

Content

For those interested in DT40, a chick pre-B-cell line proving increasingly popular for use in gene targeting projects, this is a very useful site. It features a new bursal B-cell expressed sequence tag (EST) library (Bursal EST Database), which can be directly downloaded or is searchable in situ through BLAST, both by keyword and by sequence queries. There are excellent downloadable protocols for techniques such as cell culture, transfections, chromosome preparation and immunocytochemistry, all of which are optimized for the DT40 cell line. An address book full of experienced DT40 researchers you can contact is also available. Another appealing factor is the list of 'free of charge' reagents that can be requested, including cell lines, drug-selection marker cassettes and chick genomic and cDNA libraries.

Navigation

Reporter's comments

Timeliness

This is a relatively new site that has certainly been updated within the past three months.

Best feature

The wealth of information is extremely useful to those wishing to use DT40, and the generosity in supplying really useful reagents for free is a welcome surprise.

Worst feature

The site would benefit from more in the way of a general introduction to the DT40 cell line: as it stands, it does feel as though the site starts somewhat in the middle. The picture of the lab members relaxing at the beach would, however, make one inclined to forgive them.

Wish list

An option to search the site would be helpful and being able to download from the reference list would also be a bonus.

Table of links

References

Copyright

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