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Chick pre-B cell encyclopedia
Genome Biology volume 1, Article number: reports227 (2000)
For those interested in DT40, a chick pre-B-cell line proving increasingly popular for use in gene targeting projects, this is a very useful site.
For those interested in DT40, a chick pre-B-cell line proving increasingly popular for use in gene targeting projects, this is a very useful site. It features a new bursal B-cell expressed sequence tag (EST) library (Bursal EST Database), which can be directly downloaded or is searchable in situ through BLAST, both by keyword and by sequence queries. There are excellent downloadable protocols for techniques such as cell culture, transfections, chromosome preparation and immunocytochemistry, all of which are optimized for the DT40 cell line. An address book full of experienced DT40 researchers you can contact is also available. Another appealing factor is the list of 'free of charge' reagents that can be requested, including cell lines, drug-selection marker cassettes and chick genomic and cDNA libraries.
Navigation is easy as the overall menu remains visible when you link to the pages listed and there are not too many layers to get lost in. I had no problem in downloading the Bursal EST Database or DT40 protocol files that were on offer.
This is a relatively new site that has certainly been updated within the past three months.
The wealth of information is extremely useful to those wishing to use DT40, and the generosity in supplying really useful reagents for free is a welcome surprise.
The site would benefit from more in the way of a general introduction to the DT40 cell line: as it stands, it does feel as though the site starts somewhat in the middle. The picture of the lab members relaxing at the beach would, however, make one inclined to forgive them.
An option to search the site would be helpful and being able to download from the reference list would also be a bonus.
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Curran, J. Chick pre-B cell encyclopedia. Genome Biol 1, reports227 (2000). https://doi.org/10.1186/gb-2000-1-1-reports227
- Cell Culture
- cDNA Library
- Reference List
- Sequence Query
- Chromosome Preparation