Extreme genomic erosion after recurrent demographic bottlenecks in the highly endangered Iberian lynx
- Federico Abascal†1,
- André Corvelo†2,
- Fernando Cruz†2, 3, 6,
- José L. Villanueva-Cañas4,
- Anna Vlasova5, 6,
- Marina Marcet-Houben5, 6,
- Begoña Martínez-Cruz3,
- Jade Yu Cheng7,
- Pablo Prieto5, 6,
- Víctor Quesada8,
- Javier Quilez9,
- Gang Li10,
- Francisca García11,
- Miriam Rubio-Camarillo1,
- Leonor Frias2,
- Paolo Ribeca2,
- Salvador Capella-Gutiérrez5, 6,
- José M. Rodríguez1, 12,
- Francisco Câmara5, 6,
- Ernesto Lowy13,
- Luca Cozzuto13,
- Ionas Erb5, 6,
- Michael L. Tress1,
- Jose L. Rodriguez-Ales5, 6,
- Jorge Ruiz-Orera4,
- Ferran Reverter5, 6,
- Mireia Casas-Marce3,
- Laura Soriano3,
- Javier R. Arango8,
- Sophia Derdak2,
- Beatriz Galán14,
- Julie Blanc2,
- Marta Gut2,
- Belen Lorente-Galdos9,
- Marta Andrés-Nieto15,
- Carlos López-Otín8,
- Alfonso Valencia1, 12,
- Ivo Gut2,
- José L. García14,
- Roderic Guigó5, 6, 16,
- William J. Murphy10,
- Aurora Ruiz-Herrera15, 17,
- Tomas Marques-Bonet2, 9, 18,
- Guglielmo Roma13,
- Cedric Notredame5, 6,
- Thomas Mailund7,
- M. Mar Albà4, 6, 18,
- Toni Gabaldón5, 6, 18,
- Tyler Alioto2, 6 and
- José A. Godoy3Email authorView ORCID ID profile
© The Author(s). 2016
Received: 26 February 2016
Accepted: 25 October 2016
Published: 14 December 2016
Genomic studies of endangered species provide insights into their evolution and demographic history, reveal patterns of genomic erosion that might limit their viability, and offer tools for their effective conservation. The Iberian lynx (Lynx pardinus) is the most endangered felid and a unique example of a species on the brink of extinction.
We generate the first annotated draft of the Iberian lynx genome and carry out genome-based analyses of lynx demography, evolution, and population genetics. We identify a series of severe population bottlenecks in the history of the Iberian lynx that predate its known demographic decline during the 20th century and have greatly impacted its genome evolution. We observe drastically reduced rates of weak-to-strong substitutions associated with GC-biased gene conversion and increased rates of fixation of transposable elements. We also find multiple signatures of genetic erosion in the two remnant Iberian lynx populations, including a high frequency of potentially deleterious variants and substitutions, as well as the lowest genome-wide genetic diversity reported so far in any species.
The genomic features observed in the Iberian lynx genome may hamper short- and long-term viability through reduced fitness and adaptive potential. The knowledge and resources developed in this study will boost the research on felid evolution and conservation genomics and will benefit the ongoing conservation and management of this emblematic species.
KeywordsConservation genomics Genetic diversity Inbreeding Genetic drift Lynx
Species are becoming extinct at rates unprecedented in recent history as a consequence of human activity . Surviving populations of vertebrate species have decreased in size by an average of 58% from 1970 to 2012  and 15–40% of living species are predicted to go extinct by 2050 . While the primary causes of these declines are usually known and have been the main targets of conservation efforts, genetic changes accumulated during the decline can compromise the recovery of endangered populations and limit their long-term viability. Indeed, endangered populations typically show patterns of low genetic diversity and high inbreeding that can result in loss of adaptive potential, reduced rates of reproduction and survival, and increased extinction risk . Genomic approaches are expected to improve our understanding of how the interaction between genetic drift, mutation, recombination, and natural selection shapes the genome of endangered populations and to contribute to a more effective conservation by facilitating the identification and subsequent management of deleterious variants. The fulfillment of these expectations requires, however, genomic studies in well-characterized and actively managed endangered species to serve as models .
The Iberian lynx (Lynx pardinus) is one of the four extant lynx species that share a short bobbed tail, spotted coat, muscular body, long legs, and characteristic tufted ears and beard-resembling ruffs. The Iberian and Eurasian lynx are sister species and the two extant lynxes in Eurasia, having diverged around 1.1 million years ago (Mya) [6, 7]. In contrast to the large, generalist and widespread Eurasian lynx, the Iberian lynx is smaller and a habitat- and prey-specialist, being restricted to the Mediterranean region in the Iberian Peninsula where they prey almost exclusively on rabbits. Supposed to be once fairly abundant and widely distributed across the Iberian peninsula, a steep decline during the second half of the 20th century left less than 100 lynx (less than 62 mature) distributed in the two isolated populations of Doñana and Andújar (Sierra Morena) in Andalusia, southern Spain, leading to its recognition as the most endangered felid in the world  and to its classification as “critically endangered” in the 2002, 2006, and 2008 IUCN red lists. Active conservation in the last 14 years, including in situ management of habitat, prey, and non-natural mortality, captive breeding, translocation, and reintroduction programs, has recovered lynx numbers to over 300 (156 mature) individuals in 2012, leading to its downlisting to “endangered” in the 2015 IUCN red list .
Previous studies using microsatellite markers documented low genetic diversity, a high inbreeding rate, and a high genetic differentiation between the two populations . The following evidence suggests that these genetic factors are limiting current reproduction and survival rates (inbreeding depression): (i) an increase in the proportion of abnormal sperm with individual inbreeding ; (ii) a recent decrease in litter size and survival in Doñana ; (iii) a high incidence of membranous glomerulonephritis and lymphoid depletion [13, 14]; and (iv) several deleterious traits with likely genetic bases segregate at moderate to high frequencies in the captive population, including cryptorchidism and an idiopathic epilepsy . This has prompted the translocation of individuals to reconnect the two remnant populations and their mixing in captivity, which has likely contributed to improved reproductive and survival rates. These circumstances make of the Iberian lynx a good model for the emerging field of conservation genomics .
We have sequenced, assembled, and annotated a draft genome of an Iberian male named Candiles, and re-sequenced another ten Iberian and one Eurasian lynx genomes. In addition, to obtain gene expression data and to assist gene annotation we have characterized the transcriptome of 11 lynx tissues. We use these resources to analyze the marks left by recurrent demographic bottlenecks on the dynamics of transposable elements (TEs), the rates and patterns of nucleotide substitution, and the efficiency of purifying selection. We characterize the genetic diversity in the two remnant Iberian lynx populations and discuss the interplay between demographic history, GC-biased gene conversion, genetic drift, recombination, and selection in a species on the brink of extinction.
Results and discussion
The Iberian lynx reference genome
We assembled the first draft of the Iberian lynx genome (LYPA 1.0) by combining a fosmid-pool sequencing approach  with shotgun sequencing of whole-genome fragment libraries on Illumina and 454 platforms (Additional file 1: Sections 1 and 2). With a contig N50 of 68 kb, our Iberian lynx assembly is more contiguous than other felid genome assemblies, including those of the domestic cat, tiger, and cheetah (Additional file 1: Table S4). However, due to the limited amount of long-range linkage information we were able to produce, we could not achieve as high a scaffold N50 (1.52 Mb). Regardless, the completeness of the gene space as assessed by the Core Eukaryotic Genes Mapping Approach (CEGMA)  was 95% (98% including partial genes). We annotated the reference genome with protein-coding genes and other structural and functional genomic features, including TEs and small non-coding RNAs (sncRNAs) and long non-coding RNAs (lncRNAs) (Additional file 1: Section 3). More than 98% of the 21,257 protein coding genes identified were functionally annotated with InterPro, KEGG, or Blast2GO features (Additional file 1: Section 4). We also performed a focused annotation of the degradome, the complete repertoire of proteases in the organism, with computer-assisted manual methods (Additional file 1: Section 5; Additional file 2: Datasheet S1) and analyzed expression patterns of protein-coding genes (Additional file 1: Section 6) and lncRNAs (Additional file 1: Section 7). A small percentage of these genes (<1%) were expressed in at least one tissue in the Iberian lynx but did not show homology to genes in other mammals or vertebrates (Additional file 1: Section 8; Additional file 2: Datasheet S2). These features, along with other layers of genomic information that include synteny, gene expression, and genomic variation, can be accessed interactively through a dedicated genome browser (http://denovo.cnag.cat/genomes/iberian_lynx; Additional file 1: Section 25).
Evolutionary and demographic history
The analysis with ∂a∂i was able to capture a third, more recent decline that reduced the Iberian lynx effective population size to less than 300 individuals around 315 years ago (Fig. 1c). A similarly dated bottleneck was detected previously using microsatellite marker data  and has also been suggested for the Eurasian lynx . This period is characterized by human population expansions leading to increased persecution of large carnivores, forest destruction, and expansion of agricultural land across Europe . The fourth and most recent bottleneck occurred during the 20th century. This population contraction, attributed to increased direct prosecution and decreases in main prey caused by two successive viral outbreaks, is probably too recent to be recovered by these methods but is well documented in the literature.
The Iberian lynx karyotype is identical to that of other felids in terms of diploid number (2n = 38) and G-banding  (Additional file 1: Section 11). A finer-scale analysis based on genome alignments identified five potential intra-chromosomal and ten potential inter-chromosomal rearrangements between domestic cat and lynx and up to 37 inversions. Alignments to the dog genome indicated that 20 of the inversions and six of the inter/intra-chromosomal rearrangements occurred in the Iberian lynx lineage. PCR amplification and Sanger sequencing empirically validated 8 of the 15 putative chromosomal rearrangements (five lynx-specific; Additional file 1: Section 12; Additional file 2: Datasheet S3).
We investigated the evolution of lynx genes through several complementary approaches. First, we reconstructed the molecular phylogeny of every lynx protein-coding gene in the context of 15 other mammalian species to establish orthology relationships and to detect and date duplication events  (Additional file 1: Section 13). We observed a significant enrichment in genes related to sensory perception of smell among the genes specifically expanded in the lynx lineage and also among those duplicated in the most recent common ancestor of all felids (Additional file 2: Datasheet S4), which is consistent with the importance of smell perception for these carnivores. Besides, and in contrast to humans, felids harbor multiple functional paralogs of the cysteine protease genes CTSL and CTSL2, ranging from five to ten, which are involved in extracellular matrix homeostasis  and immune regulation  (Additional file 1: Section 5; Additional file 2: Datasheet S1). Four of these new CTSL-like genes have pseudogenized in lynx but not in tiger or cat (Additional file 2: Datasheet S1). Up to 85 additional lynx genes were conservatively identified as putative pseudogenes in lynx (Additional file 2: Datasheet S5).
Second, we used the branch-site test  on a set of 9695 one-to-one orthologs from eight mammalian species (Panthera tigris, Felis catus, Lynx lynx, L. pardinus, Ailuropoda melanoleuca, Canis lupus familiaris, Homo sapiens, and Mus musculus) to identify genes that may have undergone positive selection in lynx (Additional file 1: Section 14). Following extensive manual inspection of the alignments and using strict criteria to minimize alignment errors, we identified 100 genes likely to have accumulated adaptive substitutions in the lynx lineage (Additional file 2: Datasheet S7). Felids possess outstanding hearing  and lynx in particular are attributed an exceptionally acute vision and hearing. We found two genes involved in hearing—CACNA1D (LYPA23A015140P1) and MYO1F (LYPA23A022113P1)—and two genes related to vision—OPTC (LYPA23A008195P1) and GUCY2F (LYPA23A015393P1) —among those with signatures of positive selection in the lynx lineage.
Insertion of TEs within genes is expected to be under stronger purifying selection and more so when it occurs in sense orientation with respect to the gene because sense insertion may disrupt protein translation . Consistent with this and with patterns reported for other genomes , LINEs and ERVs, but not SINEs, are particularly depleted within introns in felids and, when present, tend to be in antisense orientation with respect to the gene. Interestingly, lynx-specific LINEs show an increased proportion of insertion within genes in sense orientation (57 out of 112; 51%) compared to the genome-background frequency (37%; p = 0.004, Fisher’s exact test) (Fig. 2b). A higher proportion of in-sense LINE insertions suggests a less effective purifying selection.
Demographic bottlenecks also reduce heterozygosity and hence are expected to reduce the opportunities for GC-biased gene conversion (gBGC) . The demographic history of Iberian lynx makes it an ideal case to assess the influence of demography on gBGC. We mapped nucleotide substitutions onto the phylogeny of the domestic cat and the Iberian and Eurasian lynx using tiger as outgroup. The weak-to-strong substitution bias (from A/T to G/C; hereafter W → S), a characteristic signature of gBGC, is generally weaker in the lynx ancestor than in the domestic cat lineage and becomes drastically reduced after the evolutionary split between the Iberian and the Eurasian lynx (Additional file 1: Section 16). This drastic reduction supports a remarkable role for gBGC in shaping the evolution of genomes. Interestingly, the rate of evolution was significantly reduced in both lynx species, suggesting that under population contractions genome stasis may be increased through a decrease in gBGC.
Ratios of non-synonymous to synonymous substitution rates (dN/dS) are useful means of measuring the strength of purifying selection. Unfortunately, comparison across species is usually not possible because gene annotations differ in their qualities, diminishing the reliability of the alignments. Here, we have developed a new method to select sites aligned with the highest reliability, allowing us to conservatively filter a concatenated alignment of 8117 one-to-one orthologs from different felids, rodents, and hominids (Additional file 1: Section 13). Increased ratios in Iberian (dN/dS = 0.16) and Eurasian lynx (dN/dS = 0.17) after their divergence from their most recent common ancestor, which has a ratio similar to cat (dN/dS = 0.06), are consistent with the relaxation of purifying selection in both species. These ratios are higher than those estimated for other bottlenecked species like humans and chimpanzees (0.10 and 0.11, respectively; Fig. 3c). As most non-synonymous changes are likely to be deleterious, such high dN/dS ratios indicate a high rate of fixation of mildly deleterious mutations in both lynx species since their separation from their most recent common ancestor.
In line with the analysis of ROH, we found that the linkage disequilibrium (LD), measured as the squared correlation coefficient between genotypes in each individual (r 2 ), extends to long distances in Iberian lynx (Additional file 1: Appendix, Section 24). r 2 reaches 50% of its maximum value at a distance of 185 kb in Andújar (Fig. 4c), almost twice the average of domestic cat breeds (96 kb) and close to that observed for the highly inbred Burmese cat (249–380 kb) . An even longer extent of LD was estimated for Doñana (1.2 Mb), a result that cannot be solely attributed to its smaller sample size. It must be noted that extensive LD, an additional characteristic signature of small or inbred populations, can hinder the purging of deleterious recessive alleles  and may thus have contributed to the accumulation of mildly deleterious mutations in Iberian lynx that we observe.
Iberian lynx genetic diversity
Number of SNPs
H O per SNP
Watterson’s Θ (%)a
H E per SNP
H E per site (π) (%)a
π S (%)b
π N /π S b
To assess whether different parts of the genome might have become differentially affected by genetic drift, we analyzed genetic diversity in non-overlapping 100-kb-long windows along the genome. The X chromosome has been especially depleted of genetic variation: the average X chromosome-to-autosomal normalized diversity ratios at intergenic sites were 0.35 (standard error (SE) = 0.02), 0.29 (SE = 0.02), and 0.38 (SE = 0.03) for the global, Andújar, and Doñana populations, respectively, and ratios were even smaller for coding sequence (Additional file 1: Section 22). Ratios substantially lower than the 0.75 expected at equilibrium are predicted by theory and often observed in recently bottlenecked populations .
We also identified regions showing the highest differences in standardized heterozygosity between Eurasian and Iberian lynx (∆Z H = Z H-Eurasian − Z H-Iberian ; Additional file 1: Section 23). Windows within both the 2.5% largest negative (N = 718) and 2.5% largest positive ∆Z H (N = 671) were significantly depleted of genes (43.2 and 41.1% of outlier windows with genes, respectively, against 50.4% overall; Fisher’s exact tests, p < 0.0001) and windows with a large negative ∆Z H (Z H-Eurasian < < Z H-Iberian ) were more likely to be in subtelomeric regions (15 versus 6% overall; Fisher’s exact test, p < 0.0001). A comparatively high diversity in subtelomeric regions supports a role for recombination in maintaining genetic diversity in small or declining populations , either through an associated increase in mutation rates or by reducing the number of sites affected by hitch-hiking during positive or purifying selection. Regarding gene functions, a gene ontology enrichment analysis indicated that windows with the largest positive and the largest negative ∆Z H are both enriched for genes related to olfactory perception and G-protein signal transduction, while windows with the largest negative ∆Z H (Z H-Eurasian < < Z H-Iberian ) were also enriched in genes involved in pheromone reception, amino acid and glucose transmembrane transport, and the regulation of triglyceride biosynthesis genes, among others (Additional file 2: Datasheet S8). The olfactory receptor family is the largest and one of the most genetically diverse multigene families in vertebrates and its evolution has been suggested to be under the influence of balancing selection . Thus, despite the extreme global loss of diversity, functional variation may have been preserved at specific loci (e.g., olfactory receptor genes) by on-going balancing selection, a hypothesis that deserves further investigation.
Our analyses provided novel insights into the evolutionary and demographic history of the Iberian lynx, revealing a recent divergence and continued admixture with the Eurasian lynx and several drastic population bottlenecks in the last millennia. Such demography has shaped the patterns of nucleotide substitution and increased the fixation rate of transposable elements, whereas the predominance of genetic drift and the concomitant decrease in the efficiency of purifying selection have resulted in extremely low levels of genetic diversity and a high genetic load, indicating a severe level of genomic erosion in Iberian lynx.
The consequences of such low levels of genetic diversity for the viability of the species are hard to predict, but they are likely to limit the capacity of the Iberian lynx to adapt to environmental changes, whereas the excess of deleterious variants in combination with high inbreeding may reduce individual fitness (i.e., inbreeding depression), as suggested by recent evidence [11–15]. Current conservation efforts, including both ex situ and in situ programs, are addressing these threats by promoting the admixture of the two populations and through the genetic management of captive breeding, translocations, and reintroductions. These actions have likely contributed to the recent modest recovery of the population by generating less inbred and more genetically diverse populations that are potentially more fit.
However, these strategies cannot restore the diversity that has been definitively lost, which may limit the species adaptive potential to environmental change. Increasing the adaptability of the species would demand the careful consideration of novel but controversial genetic restoration approaches such as facilitated adaptation, genome editing, or assisted adaptive introgression [56, 57]. Although the evidence for recent natural introgression could encourage the use of Eurasian lynx as a source for the latter, such drastic measure should require the careful evaluation of hybrid fitness and the associated risks of maladaptation and hybrid swarm . In any case, existing examples of species with long-term persistence and widespread distribution despite depleted genetic diversity  allow for some measure of hope and argue for the maintenance of current conservation efforts.
The Iberian lynx draft genome, along with the other resources generated in this study, will support the species conservation by providing more informative and efficient genetic markers for genetic monitoring and management, which is currently based on 36 microsatellite markers. A selected set of highly informative SNPs will provide more reliable and cost-effective tools for genetic monitoring from non-invasive samples (e.g., scats or hairs) and more accurate estimates of relatedness and inbreeding for an integral genetic management of the species that should cover ongoing captive breeding, translocations, and reintroductions. Most importantly, these resources will facilitate the identification and eventual management of deleterious alleles of highest impact on Iberian lynx reproduction or survival.
Eleven Iberian and one Eurasian lynx were sequenced in this project (Additional file 1: Table S1). A moderately inbred male born in Andújar in 2006 and kept as a founder of the captive population since then (Candiles) was selected to provide the reference genome for the species. Ten additional Iberian and one Eurasian male were sampled for whole-genome resequencing, four of them from the population in Doñana and six from Andújar. These two populations differ in their recent demography: Doñana has remained small and isolated at least since the 1950s, while Andújar is the result of the progressive contraction of the large and more connected population of Sierra Morena . The Eurasian lynx is a male born in captivity in 2007 at the Zoológico de Córdoba (Spain) with no recent history of close inbreeding. Samples for DNA sequencing were obtained from blood and DNA was extracted following standard protocols. Ten organs (brain, heart, kidney, liver, lung, muscle, pancreas, spleen, stomach, and testes) were sampled for RNA sequencing from one of the Doñana Iberian lynx (Almoradux) immediately after its euthanization. Organ samples were immediately frozen in liquid nitrogen and kept at −80 °C. Total RNA was extracted by the RiboPure™ RNA Purification Kit (Ambion®).
Genome sequencing and assembly
Genomic DNA from a single captive male lynx (Candiles; studbook no. 0029) was isolated and shotgun-sequenced using Illumina and 454 technology in libraries of insert sizes 500 bp, 4.5 kb, and 5.2 kb. At the time, larger-insert mate-pair libraries, while desirable, were difficult to obtain and we opted for a fosmid-pool strategy . Ninety fosmid pools of 1200 clones prepared using the NxSeq 40 kb Mate-Pair Cloning Kit (Lucigen Corporation, USA) were used for fosmid-end and fosmid-pool sequencing. The 115,000 clones represented an approximately 1.6-fold physical coverage of the genome. Each pool was shotgun sequenced to greater than 100× depth and assembled independently. The resulting contigs were then merged to obtain an assembly representing approximately 67% of the estimated size of the genome. The remaining portion of the genome was assembled using whole-genome shotgun (WGS) paired-end (PE) data. Both partial assemblies were combined by scaffolding with WGS PE and mate-pair data, followed by extra scaffolding steps using RNA-seq and fosmid end data. The CEGMA pipeline was used to determine the state of the gene space as an indicator of genome completeness  (Additional file 1: Sections 1 and 2).
Total RNA was extracted by the RiboPure™ RNA Purification Kit (Ambion®) from ten organs (brain, heart, kidney, liver, lung, muscle, pancreas, spleen, stomach, and testes) sampled immediately after the euthanasia of one Iberian lynx (Almoradux) and from the blood of Candiles. Sequence libraries were prepared using the mRNA-Seq sample preparation kit (Illumina Inc., catalog number RS-100-0801). Reads were aligned to the reference assembly using GEMTools RNAseq pipeline v.1.6.2. Flux Capacitor v.1.2.4  was used to quantify genes, transcripts, exons, and splice junctions in each sample separately. Expression levels were obtained in pure read counts and in reads per kilobase per million mapped reads (RPKM) . Differential gene expression (DGE) analysis across tissues was performed with Bioconductor package edgeR v.3.4.2 (R v.3.0.2)  using classic pairwise comparison. A comparative gene expression analyses was performed using data from Brawand et al.  and RNA-seq of testis transcriptome from the domesticated cat, F. catus (Sequence Read Archive experiment ID SRX193575) using NOISeq Bioconductor package v.2.6.0  (Additional file 1: Section 6).
Transposable elements and other repeats were annotated with RepeatMasker (version open-4.0.1) , using rmblastn v2.2.27 search engine and RM database v20120418, with the F. catus library of repeats and the sensitive search option. Low-complexity regions were identified with DustMasker v.2.2.28  with default parameters. Protein-coding genes were then annotated by combining transcriptome evidence with homology-based and ab initio gene prediction methods (Additional file 1: Section 3). Ab initio gene predictions were performed on the TE-masked assembly using Genid, SGP2, GlimmerHMM, and Augustus. A combination of the Program to Assemble Spliced Alignments (PASA r2012-06-25) and Evidence Modeler (EVM r2012-06-25)  was used to obtain consensus coding sequence (CDS) models using three main sources of evidence: aligned transcripts, aligned proteins, and gene predictions. Small structured non-coding RNAs were detected using the CMsearch tool from the Infernal package (version 1.1rc2)  against the Rfam database (version 11) . Long non-coding RNAs (lncRNAs) were predicted by homology using the strategy reported in [71, 72] and by ab initio approaches using Geneid to generate a final set consisting of transcripts that are either expressed or conserved in at least one species (Additional file 1: Section 7).
We used our own automatic functional annotation pipeline based on Interproscan , KEGG , and Reactome  and Blast2GO  to assign a description (e.g., the protein name) and relevant annotation through sequence similarity and Gene Ontology-based data mining (Additional file 1: Section 4). SignalP  was used to predict the presence and location of signal peptide cleavage sites. Finally, in order to organize, store, and facilitate the access to the entire set of annotations we have developed a MySQL (http://www.mysql.com/) relational database. The modules implemented in APPRIS (http://appris.bioinfo.cnio.es/docs/appris.html) were used to map a range of conserved protein features to the splice variants annotated for each gene and to determine which of these is the main (principal) gene variant. The number of genes annotated with protein features is similar to that of the human genome, but less Iberian lynx genes align full length and without gaps to orthologs in other species or contain signal sequences. The main protein isoform could be identified for the majority of lynx genes with multiple variants (3408 of 5218 genes, 64.9%) and 8066 variants were tagged as alternative. A computer-assisted manual annotation of the degradome , the complete repertoire of proteases in the organism, found almost all of the 635 expected proteases, of which 306 were completely annotated, confirming a good gene coverage of the Iberian lynx genome (Additional file 1: Section 5).
We developed a pipeline to identify lynx orphan protein-coding genes (Additional file 1: Section 8). First, we discarded any proteins that had homologs in any of 23 non-mammalian eukaryotic species, using gene protein coding annotations from Ensembl. To search for homologs we used BlastP (2.2.23+)  with an E-value threshold of 10−4 and the filter for low complexity regions activated. Second, we discarded any proteins for which we could indirectly trace homology to other species through a second protein in lynx. This could happen, for example, if the protein had evolved very rapidly after a gene duplication event . For these searches we used BlastP with the same parameters as previously except that we used a BLOSUM80 matrix instead of the default BLOSUM62, as we were searching for sequences that had diverged relatively recently. Third, we classified the remaining proteins as lynx-specific or mammalian-specific depending on the presence of homologs in the annotated genes from F. catus, C. lupus familiaris, A. melanoleuca, Mustela putorius furo, H. sapiens, M. musculus, Bos taurus, Equus ferus caballus, and Myotis lucifugus (Ensembl version 72). Fourth, we only selected those genes expressed in at least one tissue using a RPKM threshold of 0.3. This resulted in the identification of 323 lynx-specific genes. The current gene catalogs are likely to be incomplete and this means that some of these 323 putatively lynx-specific genes may correspond to not yet annotated genes in other mammals. We thus employed published RNA-seq data for different tissues and mammalian species  to have a more comprehensive set of transcripts to compare our genes with. We ran TopHat2 v.2.0.8  for pooled-tissue reads from human, mouse, chimpanzee, macaque, and orangutan. Next, all long expressed transcripts (length >200 nucleotides) were assembled using Cufflinks (v.2.0.2)  for each species and tissue separately, not using information from gene annotations (no reference GTF file). We used Cuffmerge to obtain a comprehensive set of transcripts for each species and Cuffcompare to classify the transcripts into already known transcripts (annotated, using GTF files corresponding to Ensembl v.60) and novel transcripts (non-annotated). Finally, we ran tBlastX with an E-value threshold of 10−6 to search for homologs of the 323 putative lynx orphan genes among these transcripts. After discarding any gene that had at least one match, the list of lynx orphan genes was reduced to 204 (206 transcripts).
Evolutionary and demographic history
We extracted the autosomal contigs and obtained maximum likelihood estimates for either the isolation model of Mailund et al.  or the initial migration model of Mailund et al.  (Additional file 1: Section 9). As in Mailund et al.  we used Akaike’s information criterion (AIC) to determine the most probable model. To estimate parameters we used a Nelder-Mead optimization as implemented in scipy’s optimization module. The scripts used were “isolation-model.py” and “initial-migration-model.py” from https://github.com/mailund/IMCoalHMM. We split the autosomal contigs into 44 sets each covering ~100 Mbp and estimated the uncertainty in the parameter estimates using a leave-one-out jackknife approach. We used two complementary approaches to infer the demographic history of Iberian lynx (Additional file 1: Section 10). The first uses a pairwise sequentially Markov coalescent (PSMC) model applied to complete diploid genome sequences of single individuals to reconstruct the demographic history of the species from the distribution of the local density of heterozygous sites . The method seems to work well for periods between 10,000 to 1 million years b.p. but tends to overestimate recent population sizes and to spread sudden changes in population size over several preceding tens of thousands of years. For the second approach we used the maximum likelihood inference method implemented in the software ∂a∂i, which searches for the most recent demographic history that better fits the observed allelic frequency spectrum (AFS) . In ∂a∂i we evaluated either a single or two demographic changes, allowing them to be instantaneous or exponential and chose the best-fit model using the AIC.
Cells from a primary Iberian lynx fibroblast cell line were harvested at early passages and chromosomal preparations were obtained following standard protocols. Metaphases were stained homogenously with Leishman solution for the analysis of diploid number (2n) and the number of autosomal chromosome arms (NFa) and then G-banded with Wright’s stain following the methods described by  for karyotyping. For each staining, at least 30 metaphase spreads were analyzed. The karyotype of the Iberian lynx was arranged following the cat chromosomal nomenclature  and compared to previously published karyotypes for domestic cat and Eurasian lynx [28, 84]. For telomere detection fluorescence in situ hybridization (FISH) analysis was performed using a peptide nucleic acid (PNA) probe complementary to the telomere G-rich strand (TelC; Panagene, Yuseong-gu, Daejeon, Korea) according to the manufacturer’s protocol (Additional file 1: Section 11).
We built several pairwise alignments between lynx, cat, tiger, and dog genomes using LAST v.458 . For each scaffold, we sorted all the best-hit alignments of length >1000 bp based on the corresponding cat genome coordinates. Then, all the alignments that were less than 20 kb apart and lay on the same strand were merged with bedtools . We retained only those chained alignments that were at least 15 kbp long and for which at least 40% of the sites in the region were aligned. Finally, we explored the resulting chained alignments to detect inversions and inter/intra-chromosomal rearrangements. We used the dog genome as outgroup to determine whether the potential rearrangements took place in the cat or in the lynx branches. To filter rearrangements that may be assembly artifacts we required that at least one scaffold derived from fosmid sequencing crossed the predicted breakpoint. We tested the scaffold integrity by performing long-range PCRs with primers flanking the inferred breakpoint on the reference genome, followed by Sanger sequencing. Out of 15 potential rearrangements tested, eight were empirically validated by this approach (Additional file 2: Datasheet S3; Additional file 1: Section 12).
The Iberian lynx phylome (i.e., the complete collection of phylogenetic trees for each gene encoded in the genome) was reconstructed using the PhylomeDB pipeline . We used 15 mammalian species for expansion and pseudogene analyses and 17 for dN/dS estimation (Additional file 1: Section 13). Maximum likelihood (ML) trees were reconstructed based on the codon alignments using codonPhyML v.1.0  with GY as codon substitution model and F3X4 as model for defining the codon frequency from the alignment. The resulting phylome was used to infer orthology and paralogy relationships. For each tree, ETE v.2  was used to identify duplication and speciation nodes along the trees using a species overlap approach and a species overlap score of 0, as described by Huerta-Cepas et al. . All orthology and paralogy relationships are available through PhylomeDB . Gene Ontology enrichment analysis was performed using FatiGO . To find putative pseudogenized genes in lynx, domestic cat genes showing no homologs in the Iberian lynx genome, even when relaxing the overlap threshold to 20%, and that had homologs in at least four additional species were searched against the lynx genome using tBlastn . Cat proteins with significant (e-value <10−5) hits in the lynx genome aligning over 30% of their length were selected for further inspection. The genomic region determined by the blast search was extended by 10,000 nucleotides at both sides and exonerate-based gene prediction  was performed on the region using the cat protein as a seed; 85 predictions interrupted by stop codons were considered as putative pseudogenes.
We used 8117 sets of one-to-one orthologs comprising proteins from five carnivore species (L. pardinus, L. lynx, F. catus, P. tigris, and C. familiaris), three primates (H. sapiens, P. troglodytes, and M. mulatta), and two rodents (M. musculus and R. norvegicus) to estimate dN/dS ratios for different branches of the extended reference species tree (Fig. 3c, main text). To reduce the impact of alignment errors, the trimmed alignments used to reconstruct single gene trees were further filtered using an automated script (selective_trimming_for_dNdS_analyses.based_neighbours.py) available at the official trimAl repository in GitHub (https://github.com/scapella/trimal). Firstly, codon columns containing gaps were removed. Secondly, we scanned the corresponding translated alignments looking for columns with at least one amino acid replacement and only those surrounded by two previous and two posterior fully conserved sites were retained. Resulting alignments were concatenated and the number of nonsynonymous substitutions per nonsynonymous site (dN), synonymous substitutions per synonymous site (dS), and the corresponding dN/dS ratio (ω) were estimated for each branch using the ML method implemented in the CodeML program of PAML v.4.4. . For this analysis, we used a (1) fixed topology according to the extended species tree, (2) F3X4 as model of codon frequency, and (3) a free-omega model (model = 1) so an independent ratio for each branch is assumed.
We also produced a dated Felidae phylogeny based on filtered whole-genome alignments of available felid genomes (domestic cat, Iberian lynx, Eurasian lynx, tiger, lion, snow leopard, and cheetah; Additional file 1: Section 13.8). We analyzed the alignment using Saguaro to identify chromosomal regions with discrete phylogenetic signals that are different from the background signal  (Additional file 2: Datasheet 6), constructed a maximum likelihood tree in RAxML , and used the topology matching the species tree, which was also the most frequent one, to estimate divergence times from whole-genome alignment with MCMCtree .
We looked for signatures of positive selection in the lynx lineage using a set of 9695 one-to-one orthologs generated in the phylogenomics analyses (Additional file 1: Section 14). We selected eight different species: P. tigris, F. catus, L. lynx, L. pardinus, A. melanoleuca, C. lupus familiaris, H. sapiens, and M. musculus. We performed multiple sequence alignments with the software PRANK  and conducted a branch-site test of positive selection (PS)  using information from Timetree (http://www.timetree.org/) for the input tree. We filtered out cases with more than one site with a probability of being under positive selection higher than 0.99 by the Bayes empirical Bayes (BEB) approach as they typically corresponded to non-homologous stretches . We manually validated 100 lynx positive selection candidates (96 for Lynx sp. and four for L. lynx; Additional file 2: Datasheet S7). We used Gitools  and annotations from Ensembl version 73  to perform an enrichment analysis in the set of positively selected genes.
Genomes of lynx, cat, and tiger were pairwise aligned using LAST  with the aim of identifying orthologous regions between them (Additional file 1: Section 15). We analyzed unambiguously aligned regions for each pair of species (lynx–cat, lynx–tiger, cat–tiger) to identify strongly supported gaps. Every gap in which a particular TE covered at least 95% of the gap, 99% of that TE was within the gap, and in which target-site duplications (TSDs) were detected at each gap boundary, was considered as a species-specific TE insertion. TSD were defined by obtaining −25/+15 and −15/+25 bp around the start and end site coordinates of the TE, respectively. Then, both sequences were compared to each other with BLAST and we required that L*P/100 was greater than 6, where L is the length of the alignment and P the percentage of identity. This procedure allowed the identification of short interspersed element (SINE) and long interspersed element (LINE) insertions, as they leave clear TSDs of size ~20 bp. To analyze the accumulation of TEs along the branches of the tree that relates lynx, cat, and tiger, we relied on the pairwise comparisons between lynx and cat and between tiger and cat. Every TE insertion was mapped onto the domestic cat genome to analyze the patterns of insertion within genes.
To determine the activity of endogenous retroviruses (ERVs) in lynx, we relied on a combined approach based on synteny analyses and phylogenetic reconstruction. First, we annotated the set of endogenous retroviruses in lynx, cat, and tiger. To reconstruct full ERVs we post-processed RepeatMasker results and searched for pairs of long-terminal repeats (LTRs) that (1) were of the same type and (2) were on the same strand and (3) for which at least 50% of the LTR-enclosed sequence corresponded to ERV fragments of the same family and orientation. Finally, when ERV candidates overlapped, we retained only one of them. By doing this, we were able to reconstruct 1776, 1895, and 1940 full-ERV candidates in lynx, cat, and tiger, respectively. We built a phylogenetic tree for all these ERVs using the BioNJ method  (Additional file 1: Section 15).
To identify and polarize substitutions in Eurasian and Iberian lynxes we called variants with the RubioSeq pipeline  using the genome of domestic cat (version 6.2, felcat5) as reference (Additional file 1: Section 16). Based on the genotype of each lynx species, we selected all those sites, either variant or invariant with respect to cat, which were reliably predicted in both species in homozygosis (heterozygous sites were treated separately). The resulting dataset encompassed 2.15 billion genotyped base pairs. To infer ancestral character states, we focused on the set of sites lying on regions for which orthology was successfully established between lynx, cat, and tiger; we excluded sites lying on repeats and/or low-complexity regions. The final dataset contained 1,062,208,795 genotyped sites and included: 712,201 and 707,025 variants specific for Iberian (L. pardinus) and Eurasian (L. lynx) lynx, respectively; 9,687,075 variants shared by the two (substitutions occurring since the divergence of cat and lynxes until the divergence of Iberian and Eurasian lynxes); and 1,051,102,494 shared invariant sites. Identified substitutions were used to estimate substitution rates, non-synonymous to synonymous substitution ratios (dN/dS), and weak-to-strong (mutations from A/T to G/C; hereafter W → S) substitution biases. We translated cat genome coordinates to lynx scaffold coordinates (based on the genome alignments) to annotate the effect of substitutions on lynx protein-coding genes using SnpEff v.3.5  and based on the principal transcript isoforms identified with APPRIS . Substitutions were condensed into non-overlapping 100-kbp windows containing at least 10,000 informative sites to analyze the patterns of evolution along chromosomes.
We detected segmental duplications (SD) in the genomes of one Eurasian lynx (L. lynx) and 11 Iberian lynxes (L. pardinus) both from Sierra Morena (7) and from Doñana (4) (Additional file 1: Section 17). Illumina 100-bp reads were mapped to the repeat-masked Fca-6.2 (UCSC felCat5) domestic cat assembly using BWA  (using as parameters “bwa -q 15”) and duplicated reads were removed with SAMtools . Successfully mapped reads in the resulting BAM files were then used to recover the original FASTQ files using the bam2fastq tool (http://gsl.hudsonalpha.org/information/software/bam2fastq). The final set of 100-bp reads were clipped to 36-bp fragments but only retaining positions in the read with high quality, which was assessed with fastqc (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). The resulting 36-bp reads were then mapped to the reference assembly using mrFast  (using as mapping parameters “–e 2”). mrCaNaVaR (v.3.0.1)  was used to estimate the copy number along the genome from the mapping read depth. Mean read depth per base pair was calculated in 1-kbp non-overlapping windows and a control read depth distribution was obtained by iteratively excluding windows with extreme read depth values relative to the normal distribution. The mean read depth in these control regions was considered to correspond to copy number equal to two and was used to convert the read depth value in each window into a GC-corrected absolute copy number in each sample. We called SDs in each individual as genomic regions in which the predicted copy number significantly exceeded diploidy, while accounting for the technical variation in the copy number predictions across samples. Finally, we filtered out SDs shorter than 10 kbp and with >85% of their size overlapping with repeats.
To generate variation data for population genomics and species divergence analyses we re-sequenced ten Iberian (mean depth 26.4×) and one Eurasian lynx (64×) (Additional file 1: Section 18). Variant discovery and genotyping were performed using a mapping-based approach as implemented in the RUbioSeq suite , using either the Iberian lynx or the domestic cat genome as references and different sets of samples. In addition, we applied the reference-free and assembly-based strategy implemented in Cortex_var . The two procedures yielded largely concordant results but, as expected by its higher sensitivity to detect singletons, the mapping-based calling dataset yielded more variants, slightly higher diversity estimates, and more reliable estimates of LD and homozygosity blocks and was the one used for population genomic analyses.
We used VCFTools v.0.1.10  to identify runs of homozygosity (ROH) in each Iberian lynx individual, to estimate individual inbreeding coefficients from the extent of ROH larger than 1 Mbp (F roh ) and from the observed homozygosities and allele frequencies (F h) (Additional file 1: Section 19), and to compute diversity and differentiation parameters (Additional file 1: Section 20). Estimates were averaged for all variants and converted to per-site averages using the number of reliably called invariant sites. For coding sequences we counted synonymous and non-synonymous variants as annotated by SnpEff v.3.5  (P s , P n ) and calculated the per-site synonymous and non-synonymous nucleotide diversity (πS, πN) by assuming that three-quarters of all of the sites are non-synonymous. The genomic averages of πS and πN were calculated by averaging across CDS with more than 200 callable sites weighted by the number of sites; genomic ratios were calculated from these averages (Additional file 1: Section 21). To study the distribution of diversity along and across chromosomes, we considered genomic regions with conserved synteny to the domestic cat genome (felCat 6.2) so that we could assign lynx regions to specific chromosomal locations and obtain estimates of cat–lynx divergence (Additional file 1: Sections 22 and 23). We excluded repeats, low complexity regions, centromeres, and telomeres along with 2 Mb of flanking regions, and the pseudoautosomal region 1 (PAR1) in the X chromosome (first 6 Mb), and defined non-overlapping 100-kb-long syntenic windows. For each of these windows we estimated the nucleotide diversity (π, nucleotide diversity) and the divergence to cat (D, the observed fraction of fixed differences), and the ratio between the two (π/D) was used as a measure of diversity normalized by mutation rate. Standard errors were calculated by bootstrapping over windows or CDS as implemented in the boot package for R [110, 111] to account for the correlation among nearby sites due to LD (Additional file 1: Section 22).
To characterize genomic patterns of diversity in the Iberian lynx genome in comparison to the Eurasian lynx genome, for each window we calculated the Z-transformed per-site average of the observed heterozygosity in Eurasian (Z H-EL ) and in Iberian lynx (Z H-IL ) and its difference (∆Z H = Z H-EL − Z H-IL ). Windows with a ∆Z H value higher than the 99.9th or equal to or lower than the 0.1th percentile were identified as outliers. We then tested whether outlier windows were preferentially located in subtelomeric regions (within 5 Mb from the end of chromosomes) or were significantly enriched for particular cellular components, biological processes, or molecular functions by performing a Gene Ontology analyses using FatiGO , as implemented in Babelomics 4.3 .
Iberian lynx samples were contributed by the Iberian lynx Ex Situ Breeding Programme, the Centro de Análisis y Diagnóstico de la Fauna Silvestre, and the LIFE projects LIFE06NAT/E/000209 and LIFE10NAT/ES/570, with authorization of Consejería de Medio Ambiente de la Junta de Andalucía. The Eurasian lynx sample was donated by the Zoológico de Córdoba, Spain. Roche Diagnostics contributed mate-pair 454 sequence data. Centro de Supercomputación de Galicia (CESGA) provided computation and storage services. We thank our NCBI/NIH colleagues Richa Agarwala, Sergey Shiryev, and Aleksandr Morgulis for generating a reference-assisted assembly and its alignment to the domestic cat genome, which encouraged and inspired the assembly approach we finally implemented. We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). We also thank the two anonymous reviewers whose comments and suggestions helped improve this manuscript.
The financial support for this work was provided by Banco de Santander, CSIC, and Fundación General CSIC through a “Proyectos Cero” grant. FC and BM-C were hired under the EcoGenes project funded by the European FP7 program (264125-FP7-REGPOT-2010-1) and AVl and FCa under the INB Project PT13/0001/0021 (ISCIII - Subdirección General de Evaluación y Fomento de la Investigación/FEDER “Una Manera de hacer Europa”). EBD-CSIC and CRG received support from the Spanish Ministry of Economy and Competitiveness under the “Centro de Excelencia Severo Ochoa 2013-2017” program, grants SEV-2012-0262 and SEV-2012-0208.
Availability of data and materials
The datasets supporting the conclusions of this article are available in the European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) as study PRJEB12609. A browser for the Iberian lynx genome assembly is available at http://denovo.cnag.cat/genomes/iberian_lynx.
JAG led the project and the manuscript preparation. JAG, JLG, RG, and IG designed and managed the project and together with TA, TG, CN, MMA, TM-B, CL-O, AVa, AR-H, TM, and WJM supervised subprojects/tasks. BAC and fosmid library generation: BG and JLG*. Illumina sequencing production: JB, MG, IG*. Whole genome assembly: AC, LF, PR, and TA*. Mitochondrial genome assembly: MC-M, AC, JAG, and TA*. Y chromosome assembly and annotation: GL and WJM*. Protein-coding gene annotation: FCa, AC, and TA*. Functional annotation: AVl, GR, EL, and RG*. Splicing isoforms and annotation evaluation: JMR and MT*. Orphan genes: JLV-C, JR-O, and MMA*. Degradome characterization: VQ, JRA, and CL-O*. sncRNA annotation: LC and EL. Gene expression analyses: AVl, FR, JLV-C, MMA, and RG*. lncRNA annotation and expression: PP, IE, and CN*. Eurasian and Iberian lynx divergence: JYC and TM*. Demographic history: FCr*, BM-C*, and JAG. Karyotype characterization: FG, MA-N, and AR-H*. Synteny analyses: FA*, MR-C, BG, and JAG. Phylogenomic analyses: MM-H, SC-G, JLR-A, and TG*; GL and WJM*. Positive selection: JLV-C, JR-O, MM-H, FA, JAG, and MMA* Transposable element annotation and dynamics: FA* and MR-C. Substitution patterns: FA* and FCr. Segmental duplications and structural variation: JQ, BL-G, and TM-B*. Variant discovery and genotyping: FCr*, MR-C, and SD. Population genomics: FCr, FA, BM-C, LS, and JAG*. Lynx genome browser: AC, TA, and PR*. Asterisks indicate task leaders. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
We exclusively used samples collected by expert veterinarians during the course of routine check-ups of captive animals, revisions of animals captured for other reasons, or from necropsies. No animals were trapped or sacrificed for the purposes of this study and therefore a formal approval by an Institutional Animal Care and Use Committee was not necessary. Samples were used for this study under permits of the Consejería de Medio Ambiente of the Junta de Andalucía (Andalusian Government).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Pimm SL, Jenkins CN, Abell R, Brooks TM, Gittleman JL, Joppa LN, Raven PH, Roberts CM, Sexton JO. The biodiversity of species and their rates of extinction, distribution, and protection. Science. 2014;344:1246752.PubMedView ArticleGoogle Scholar
- WWF. Living Planet Report 2016. Risk and resilience in a new era. Gland: WWF International. 2016:p.145.Google Scholar
- Thomas CD, Williamson M. Extinction and climate change. Nature. 2012;482:E4–5.PubMedView ArticleGoogle Scholar
- Frankham R, Ballou JD, Briscoe DA. Introduction to conservation genetics. 2nd ed. New York: Cambridge University Press; 2010.View ArticleGoogle Scholar
- Shafer ABA, Wolf JBW, Alves PC, Bergstrom L, Bruford MW, Brannstrom I, Colling G, Dalen L, De Meester L, Ekblom R, et al. Genomics and the challenging translation into conservation practice. Trends Ecol Evol. 2015;30:78–87.PubMedView ArticleGoogle Scholar
- Li G, Davis BW, Eizirik E, Murphy WJ. Phylogenomic evidence for ancient hybridization in the genomes of living cats (Felidae). Genome Res. 2016;26:1–11.PubMedPubMed CentralView ArticleGoogle Scholar
- Johnson WE, Eizirik E, Pecon-Slattery J, Murphy WJ, Antunes A, Teeling E, O’Brien SJ. The Late Miocene radiation of modern Felidae: a genetic assessment. Science. 2006;311:73–7.PubMedView ArticleGoogle Scholar
- Nowell K, Jackson P, IUCN/SSC Cat Specialist Group. Wild cats: status survey and conservation action plan. Gland: IUCN; 1996.Google Scholar
- Lynx pardinus. The IUCN red list of threatened species. http://www.iucnredlist.org. Accessed 10 Sept 2016.
- Casas-Marce M, Soriano L, López-Bao JV, Godoy JA. Genetics at the verge of extinction: insights from the Iberian lynx. Mol Ecol. 2013;22:5503–15.PubMedView ArticleGoogle Scholar
- Ruiz-López MJ, Gañán N, Godoy JA, Del Olmo A, Garde J, Espeso G, Vargas A, Martínez F, Roldán ERS, Gomendio M. Heterozygosity-fitness correlations and inbreeding depression in two critically endangered mammals. Conserv Biol. 2012;26:1121–9.PubMedView ArticleGoogle Scholar
- Palomares F, Godoy JA, López-Bao JV, Rodríguez A, Roques S, Casas-Marce M, Revilla E, Delibes M. Possible extinction vortex for a population of Iberian lynx on the verge of extirpation. Conserv Biol. 2012;26:689–97.PubMedView ArticleGoogle Scholar
- Peña L, García P, Jiménez MA, Benito A, Alenza MDP, Sánchez B. Histopathological and immunohistochemical findings in lymphoid tissues of the endangered Iberian lynx (Lynx pardinus). Comp Immunol Microbiol Infect Dis. 2006;29:114–26.PubMedView ArticleGoogle Scholar
- Jiménez MA, Sánchez B, Alenza MDP, García P, López JV, Rodríguez A, Muñoz A, Martínez F, Vargas A, Peña L. Membranous glomerulonephritis in the Iberian lynx (Lynx pardinus). Vet Immunol Immunopathol. 2008;121:34–43.PubMedView ArticleGoogle Scholar
- Martínez F, Manteca X, Pastor J. Retrospective study of morbidity and mortality of captive Iberian lynx (Lynx pardinus) in the ex situ conservation programme (2004-june 2010). J Zoo Wildl Med. 2013;44:845–52.PubMedView ArticleGoogle Scholar
- Steiner CC, Putnam AS, Hoeck PEA, Ryder OA. Conservation genomics of threatened animal species. Annu Rev An Biosci. 2013;1:261–81.View ArticleGoogle Scholar
- Kitzman JO, MacKenzie AP, Adey A, Hiatt JB, Patwardhan RP, Sudmant PH, Ng SB, Alkan C, Qiu RL, Eichler EE, Shendure J. Haplotype-resolved genome sequencing of a Gujarati Indian individual. Nat Biotechnol. 2011;29:59–63.PubMedView ArticleGoogle Scholar
- Parra G, Bradnam K, Ning Z, Keane T, Korf I. Assessing the gene space in draft genomes. Nucleic Acids Res. 2009;37:289–97.PubMedView ArticleGoogle Scholar
- Mailund T, Dutheil JY, Hobolth A, Lunter G, Schierup MH. Estimating divergence time and ancestral effective population size of Bornean and Sumatran orangutan subspecies using a coalescent hidden Markov model. PLoS Genet. 2011;7:e1001319.PubMedPubMed CentralView ArticleGoogle Scholar
- Mailund T, Halager AE, Westergaard M, Dutheil JY, Munch K, Andersen LN, Lunter G, Prufer K, Scally A, Hobolth A, Schierup MH. A new isolation with migration model along complete genomes infers very different divergence processes among closely related great ape species. PLoS Genet. 2012;8:e1003125.PubMedPubMed CentralView ArticleGoogle Scholar
- Li H, Durbin R. Inference of human population history from individual whole-genome sequences. Nature. 2011;475:493–6.PubMedPubMed CentralView ArticleGoogle Scholar
- Gutenkunst RN, Hernandez RD, Williamson SH, Bustamante CD. Inferring the joint demographic history of multiple populations from multidimensional SNP frequency data. PLoS Genet. 2009;5:e1000695.PubMedPubMed CentralView ArticleGoogle Scholar
- Clavero M, Delibes M. Using historical accounts to set conservation baselines: the case of lynx species in Spain. Biodivers Conserv. 2013;22:1691–702.View ArticleGoogle Scholar
- Rodríguez-Varela R, Tagliacozzo A, Ureña I, García N, Crégut-Bonnoure E, Mannino MA, Arsuaga JL, Valdiosera C. Ancient DNA evidence of Iberian lynx palaeoendemism. Quaternary Sci Rev. 2015;112:172–80.View ArticleGoogle Scholar
- Koen EL, Bowman J, Lalor JL, Wilson PJ. Continental-scale assessment of the hybrid zone between bobcat and Canada lynx. Biol Conserv. 2014;178:107–15.View ArticleGoogle Scholar
- Schmidt K, Ratkiewicz M, Konopinski MK. The importance of genetic variability and population differentiation in the Eurasian lynx Lynx lynx for conservation, in the context of habitat and climate change. Mamm Rev. 2011;41:112–24.View ArticleGoogle Scholar
- Ellis EC, Goldewijk KK, Siebert S, Lightman D, Ramankutty N. Anthropogenic transformation of the biomes, 1700 to 2000. Global Ecol Biogeogr. 2010;19:589–606.Google Scholar
- Nie W, Wang J, Su W, Wang D, Tanomtong A, Perelman PL, Graphodatsky AS, Yang F. Chromosomal rearrangements and karyotype evolution in carnivores revealed by chromosome painting. Heredity. 2012;108:17–27.PubMedView ArticleGoogle Scholar
- Huerta-Cepas J, Gabaldón T. Assigning duplication events to relative temporal scales in genome-wide studies. Bioinformatics. 2011;27:38–45.PubMedView ArticleGoogle Scholar
- Lombardi G, Burzyn D, Mundinano J, Berguer P, Bekinschtein P, Costa H, Castillo LF, Goldman A, Meiss R, Piazzon I, Nepomnaschy I. Cathepsin-L influences the expression of extracellular matrix in lymphoid organs and plays a role in the regulation of thymic output and of peripheral T cell number. J Immunol. 2005;174:7022–32.PubMedView ArticleGoogle Scholar
- Schurigt U, Eilenstein R, Gajda M, Leipner C, Sevenich L, Reinheckel T, Peters C, Wiederanders B, Brauer R. Decreased arthritis severity in cathepsin L-deficient mice is attributed to an impaired T helper cell compartment. Inflammation Res. 2012;61:1021–9.View ArticleGoogle Scholar
- Yang ZH. PAML 4: phylogenetic analysis by maximum likelihood. Mol Biol Evol. 2007;24:1586–91.PubMedView ArticleGoogle Scholar
- Heffner HE, Heffner RS. The sound-localization ability of cats. J Neurophysiol. 2005;94:3653–3.PubMedView ArticleGoogle Scholar
- Hunt DM, Buch P, Michaelides M. Guanylate cyclases and associated activator proteins in retinal disease. Mol Cell Biochem. 2010;334:157–68.PubMedView ArticleGoogle Scholar
- Boutin TS, Le Rouzic A, Capy P. How does selfing affect the dynamics of selfish transposable elements? Mob DNA. 2012;3:5.PubMedPubMed CentralView ArticleGoogle Scholar
- Lynch M, Conery JS. The origins of genome complexity. Science. 2003;302:1401–4.PubMedView ArticleGoogle Scholar
- Morgan MT. Transposable element number in mixed mating populations. Genet Res. 2001;77:261–75.PubMedView ArticleGoogle Scholar
- Gherman A, Chen PE, Teslovich TM, Stankiewicz P, Withers M, Kashuk CS, Chakravarti A, Lupski JR, Cutler DJ, Katsanis N. Population bottlenecks as a potential major shaping force of human genome architecture. PLoS Genet. 2007;3:e119.PubMedPubMed CentralView ArticleGoogle Scholar
- Lockton S, Ross-Ibarra J, Gaut BS. Demography and weak selection drive patterns of transposable element diversity in natural populations of Arabidopsis lyrata. Proc Natl Acad Sci U S A. 2008;105:13965–70.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang Y, Romanish MT, Mager DL. Distributions of transposable elements reveal hazardous zones in mammalian introns. PLoS Comp Biol. 2011;7:e1002046.View ArticleGoogle Scholar
- Glemin S, Arndt PF, Messer PW, Petrov D, Galtier N, Duret L. Quantification of GC-biased gene conversion in the human genome. Genome Res. 2015;25:1215–28.PubMedPubMed CentralView ArticleGoogle Scholar
- Katzman S, Kern AD, Pollard KS, Salama SR, Haussler D. GC-biased evolution near human accelerated regions. PLoS Genet. 2010;6:e1000960.PubMedPubMed CentralView ArticleGoogle Scholar
- Cho YS, Hu L, Hou H, Lee H, Xu J, Kwon S, Oh S, Kim H-M, Jho S, Kim S, et al. The tiger genome and comparative analysis with lion and snow leopard genomes. Nat Commun. 2013;4:2433.PubMedPubMed CentralGoogle Scholar
- Li S, Li B, Cheng C, Xiong Z, Liu Q, Lai J, Carey H, Zhang Q, Zheng H, Wei S, et al. Genomic signatures of near-extinction and rebirth of the crested ibis and other endangered bird species. Genome Biol. 2014;15:557.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhao S, Zheng P, Dong S, Zhan X, Wu Q, Guo X, Hu Y, He W, Zhang S, Fan W, et al. Whole-genome sequencing of giant pandas provides insights into demographic history and local adaptation. Nat Genet. 2013;45:67–71.PubMedView ArticleGoogle Scholar
- Xue Y, Prado-Martínez J, Sudmant PH, Narasimhan V, Ayub Q, Szpak M, Frandsen P, Chen Y, Yngvadottir B, Cooper DN, et al. Mountain gorilla genomes reveal the impact of long-term population decline and inbreeding. Science. 2015;348:242–5.PubMedPubMed CentralView ArticleGoogle Scholar
- Der Sarkissian C, Ermini L, Schubert M, Yang Melinda A, Librado P, Fumagalli M, Jónsson H, Bar-Gal Gila K, Albrechtsen A, Vieira Filipe G, et al. Evolutionary genomics and conservation of the endangered Przewalski’s horse. Curr Biol. 2015;25:2577–83.View ArticleGoogle Scholar
- Alhaddad H, Khan R, Grahn RA, Gandolfi B, Mullikin JC, Cole SA, Gruffydd-Jones TJ, Haggstrom J, Lohi H, Longeri M, Lyons LA. Extent of linkage disequilibrium in the domestic cat, Felis silvestris catus, and its breeds. Plos One. 2013;8:e53537.PubMedPubMed CentralView ArticleGoogle Scholar
- Hill WG, Robertson A. The effect of linkage on limits to artificial selection. Genet Res. 1966;8:269–94.PubMedView ArticleGoogle Scholar
- Leffler EM, Bullaughey K, Matute DR, Meyer WK, Segurel L, Venkat A, Andolfatto P, Przeworski M. Revisiting an old riddle: what determines genetic diversity levels within species? PLoS Biol. 2012;10:e1001388.PubMedPubMed CentralView ArticleGoogle Scholar
- Bustamante CD, Fledel-Alon A, Williamson S, Nielsen R, Hubisz MT, Glanowski S, Tanenbaum DM, White TJ, Sninsky JJ, Hernandez RD, et al. Natural selection on protein-coding genes in the human genome. Nature. 2005;437:1153–7.PubMedView ArticleGoogle Scholar
- Loire E, Chiari Y, Bernard A, Cahais V, Romiguier J, Nabholz B, Lourenco JM, Galtier N. Population genomics of the endangered giant Galapagos tortoise. Genome Biol. 2013;14:R136.PubMedPubMed CentralView ArticleGoogle Scholar
- Pool JE, Nielsen R. Population size changes reshape genomic patterns of diversity. Evolution. 2007;61:3001–6.PubMedPubMed CentralView ArticleGoogle Scholar
- Otto SP, Barton NH. Selection for recombination in small populations. Evolution. 2001;55:1921–31.PubMedView ArticleGoogle Scholar
- Alonso S, López S, Izagirre N, de la Rua C. Overdominance in the human genome and olfactory receptor activity. Mol Biol Evol. 2008;25:997–1001.PubMedView ArticleGoogle Scholar
- Hamilton JA, Miller JM. Adaptive introgression as a resource for management and genetic conservation in a changing climate. Conserv Biol. 2016;30:33–41.PubMedView ArticleGoogle Scholar
- Thomas MA, Roemer GW, Donlan CJ, Dickson BG, Matocq M, Malaney J. Gene tweaking for conservation. Nature. 2013;501:485–6.PubMedView ArticleGoogle Scholar
- Kovach RP, Luikart G, Lowe WH, Boyer MC, Muhlfeld CC. Risk and efficacy of human-enabled interspecific hybridization for climate-change adaptation: response to Hamilton and Miller (2016). Conserv Biol. 2016;30:428–30.PubMedView ArticleGoogle Scholar
- Reed DH. Albatrosses, eagles and newts, Oh My!: exceptions to the prevailing paradigm concerning genetic diversity and population viability? Anim Conserv. 2010;13:448–57.View ArticleGoogle Scholar
- Palomares F, Rodríguez A, Revilla E, López-Bao JV, Calzada J. Assessment of the conservation efforts to prevent extinction of the Iberian lynx. Conserv Biol. 2011;25:4–8.PubMedView ArticleGoogle Scholar
- Griebel T, Zacher B, Ribeca P, Raineri E, Lacroix V, Guigo R, Sammeth M. Modelling and simulating generic RNA-Seq experiments with the flux simulator. Nucleic Acids Res. 2012;40:10073–83.PubMedPubMed CentralView ArticleGoogle Scholar
- Ramskold D, Wang ET, Burge CB, Sandberg R. An abundance of ubiquitously expressed genes revealed by tissue transcriptome sequence data. PLoS Comp Biol. 2009;5:e1000598.View ArticleGoogle Scholar
- Robinson MD, McCarthy DJ, Smyth GK. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 2010;26:139–40.PubMedView ArticleGoogle Scholar
- Brawand D, Soumillon M, Necsulea A, Julien P, Csardi G, Harrigan P, Weier M, Liechti A, Aximu-Petri A, Kircher M, et al. The evolution of gene expression levels in mammalian organs. Nature. 2011;478:343–8.PubMedView ArticleGoogle Scholar
- Tarazona S, Garcia-Alcalde F, Dopazo J, Ferrer A, Conesa A. Differential expression in RNA-seq: a matter of depth. Genome Res. 2011;21:2213–23.PubMedPubMed CentralView ArticleGoogle Scholar
- RepeatMasker Open-4.0. http://www.repeatmasker.org.
- Morgulis A, Gertz EM, Schäffer AA, Agarwala R. A fast and symmetric DUST implementation to mask low-complexity DNA sequences. J Comput Biol. 2006;13:1028–40.PubMedView ArticleGoogle Scholar
- Haas BJ, Salzberg SL, Zhu W, Pertea M, Allen JE, Orvis J, White O, Buell CR, Wortman JR. Automated eukaryotic gene structure annotation using EVidenceModeler and the Program to Assemble Spliced Alignments. Genome Biol. 2008;9:R7.PubMedPubMed CentralView ArticleGoogle Scholar
- Nawrocki EP, Kolbe DL, Eddy SR. Infernal 1.0: inference of RNA alignments. Bioinformatics. 2009;25:1335–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Griffiths-Jones S, Moxon S, Marshall M, Khanna A, Eddy SR, Bateman A. Rfam: annotating non-coding RNAs in complete genomes. Nucleic Acids Res. 2005;33:D121–4.PubMedView ArticleGoogle Scholar
- Esteve-Codina A, Kofler R, Palmieri N, Bussotti G, Notredame C, Pérez-Enciso M. Exploring the gonad transcriptome of two extreme male pigs with RNA-seq. BMC Genomics. 2011;12:552.Google Scholar
- Derrien T, Johnson R, Bussotti G, Tanzer A, Djebali S, Tilgner H, Guernec G, Martin D, Merkel A, Knowles DG, et al. The GENCODE v7 catalog of human long noncoding RNAs: Analysis of their gene structure, evolution, and expression. Genome Res. 2012;22:1775–89.PubMedPubMed CentralView ArticleGoogle Scholar
- Zdobnov EM, Apweiler R. InterProScan--an integration platform for the signature-recognition methods in InterPro. Bioinformatics. 2001;17:847–8.PubMedView ArticleGoogle Scholar
- Kanehisa M, Goto S, Sato Y, Furumichi M, Tanabe M. KEGG for integration and interpretation of large-scale molecular data sets. Nucleic Acids Res. 2012;40:D109–14.PubMedView ArticleGoogle Scholar
- Matthews L, Gopinath G, Gillespie M, Caudy M, Croft D, de Bono B, Garapati P, Hemish J, Hermjakob H, Jassal B, et al. Reactome knowledgebase of human biological pathways and processes. Nucleic Acids Res. 2009;37:D619–22.PubMedView ArticleGoogle Scholar
- Gotz S, Garcia-Gómez JM, Terol J, Williams TD, Nagaraj SH, Nueda MJ, Robles M, Talon M, Dopazo J, Conesa A. High-throughput functional annotation and data mining with the Blast2GO suite. Nucleic Acids Res. 2008;36:3420–35.PubMedPubMed CentralView ArticleGoogle Scholar
- Petersen TN, Brunak S, von Heijne G, Nielsen H. SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods. 2011;8:785–6.PubMedView ArticleGoogle Scholar
- Ordoñez GR, Puente XS, Quesada V, López-Otín C. Proteolytic systems: constructing degradomes. Met Mol Biol. 2009;539:33–47.View ArticleGoogle Scholar
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215:403–10.PubMedView ArticleGoogle Scholar
- Toll-Riera M, Bosch N, Bellora N, Castelo R, Armengol L, Estivill X, Albà MM. Origin of primate orphan genes: A comparative genomics approach. Mol Biol Evol. 2009;26:603–12.PubMedView ArticleGoogle Scholar
- Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, Salzberg SL, Wold BJ, Pachter L. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010;28:511–5.PubMedPubMed CentralView ArticleGoogle Scholar
- Seabright M. Use of proteolytic-enzymes for mapping of structural rearrangements in chromosomes of man. Chromosoma. 1972;36:204.PubMedView ArticleGoogle Scholar
- Wursterhill DH, Centerwall WR. The interrelationships of chromosome-banding patterns in canids, mustelids, hyena, and felids. Cytogenet Cell Genet. 1982;34:178–92.View ArticleGoogle Scholar
- O'Brien JT, Menninger JC, Nash WG. Atlas of mammalian chromosomes. Hoboken: Wiley; 2006.View ArticleGoogle Scholar
- Kiełbasa SM, Wan R, Sato K, Horton P, Frith MC. Adaptive seeds tame genomic sequence comparison. Genome Res. 2011;21:487–93.PubMedPubMed CentralView ArticleGoogle Scholar
- Quinlan AR, Hall IM. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics. 2010;26:841–2.PubMedPubMed CentralView ArticleGoogle Scholar
- Huerta-Cepas J, Capella-Gutiérrez S, Pryszcz LP, Denisov I, Kormes D, Marcet-Houben M, Gabaldón T. PhylomeDB v3.0: an expanding repository of genome-wide collections of trees, alignments and phylogeny-based orthology and paralogy predictions. Nucleic Acids Res. 2011;39:D556–60.PubMedView ArticleGoogle Scholar
- Gil M, Zanetti MS, Zoller S, Anisimova M. CodonPhyML: fast maximum likelihood phylogeny estimation under codon substitution models. Mol Biol Evol. 2013;30:1270–80.PubMedPubMed CentralView ArticleGoogle Scholar
- Huerta-Cepas J, Dopazo J, Gabaldón T. ETE: a python environment for tree exploration. BMC Bioinf. 2010;11:24–4.View ArticleGoogle Scholar
- Huerta-Cepas J, Dopazo H, Dopazo J, Gabaldón T. The human phylome. Genome Biol. 2007;8:R109–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Huerta-Cepas J, Capella-Gutiérrez S, Pryszcz LP, Marcet-Houben M, Gabaldón T. PhylomeDB v4: zooming into the plurality of evolutionary histories of a genome. Nucleic Acids Res. 2014;42:D897–902.PubMedView ArticleGoogle Scholar
- Al-Shahrour F, Minguez P, Tárraga J, Medina I, Alloza E, Montaner D, Dopazo J. FatiGO +: a functional profiling tool for genomic data. Integration of functional annotation, regulatory motifs and interaction data with microarray experiments. Nucleic Acids Res. 2007;35:W91–W96.Google Scholar
- Slater GS, Birney E. Automated generation of heuristics for biological sequence comparison. BMC Bioinf. 2005;6:e31.View ArticleGoogle Scholar
- Zamani N, Russell P, Lantz H, Hoeppner MP, Meadows JRS, Vijay N, Mauceli E, di Palma F, Lindblad-Toh K, Jern P, Grabherr MG. Unsupervised genome-wide recognition of local relationship patterns. BMC Genomics. 2013;14:347.Google Scholar
- Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 2014;30:1312–3.PubMedPubMed CentralView ArticleGoogle Scholar
- Loytynoja A, Goldman N. An algorithm for progressive multiple alignment of sequences with insertions. Proc Natl Acad Sci U S A. 2005;102:10557–62.PubMedPubMed CentralView ArticleGoogle Scholar
- Villanueva-Cañas JL, Laurie S, Albà MM. Improving genome-wide scans of positive selection by using protein isoforms of similar length. Genome Biol Evol. 2013;5:457–67.PubMedPubMed CentralView ArticleGoogle Scholar
- Pérez-Llamas C, López-Bigas N. Gitools: Analysis and visualisation of genomic data using interactive heat-maps. PLoS One. 2011;6:e19541.PubMedPubMed CentralView ArticleGoogle Scholar
- Flicek P, Ahmed I, Amode MR, Barrell D, Beal K, Brent S, Carvalho-Silva D, Clapham P, Coates G, Fairley S, et al. Ensembl 2013. Nucleic Acids Res. 2013;41:D48–55.PubMedView ArticleGoogle Scholar
- Gascuel O. BIONJ: An improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol. 1997;14:685–95.PubMedView ArticleGoogle Scholar
- Rubio-Camarillo M, Gómez-López G, Fernández JM, Valencia A, Pisano DG. RUbioSeq: a suite of parallelized pipelines to automate exome variation and bisulfite-seq analyses. Bioinformatics. 2013;29:1687–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Cingolani P, Platts A, Wang LL, Coon M, Nguyen T, Wang L, Land SJ, Lu XY, Ruden DM. A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w(1118); iso-2; iso-3. Fly. 2012;6:80–92.PubMedPubMed CentralView ArticleGoogle Scholar
- Rodríguez JM, Maietta P, Ezkurdia I, Pietrelli A, Wesselink J-J, López G, Valencia A, Tress ML. APPRIS: annotation of principal and alternative splice isoforms. Nucleic Acids Res. 2013;41:D110–7.PubMedView ArticleGoogle Scholar
- Li H, Durbin R. Fast and accurate short read alignment with Burrows–Wheeler transform. Bioinformatics. 2009;25:1754–60.PubMedPubMed CentralView ArticleGoogle Scholar
- Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R, Proc GPD. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009;25:2078–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Hach F, Sarrafi I, Hormozdiari F, Alkan C, Eichler EE, Sahinalp SC. mrsFAST-Ultra: a compact, SNP-aware mapper for high performance sequencing applications. Nucleic Acids Res. 2014;42:W494–500.PubMedPubMed CentralView ArticleGoogle Scholar
- Alkan C, Kidd JM, Marques-Bonet T, Aksay G, Antonacci F, Hormozdiari F, Kitzman JO, Baker C, Malig M, Mutlu O, et al. Personalized copy number and segmental duplication maps using next-generation sequencing. Nat Genet. 2009;41:1061–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Iqbal Z, Caccamo M, Turner I, Flicek P, McVean G. De novo assembly and genotyping of variants using colored de Bruijn graphs. Nat Genet. 2012;44:226–32.PubMedPubMed CentralView ArticleGoogle Scholar
- Danecek P, Auton A, Abecasis G, Albers CA, Banks E, DePristo MA, Handsaker RE, Lunter G, Marth GT, Sherry ST, et al. The variant call format and VCFtools. Bioinformatics. 2011;27:2156–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Davinson AC, Hinkley DV. Bootstrap methods and their application. Cambridge: Cambridge University Press; 1997.View ArticleGoogle Scholar
- Canty A, Ripley B. boot: Bootstrap R (S-Plus) Functions. R package version 1.3-11. 2014.Google Scholar
- Al-Shahrour F, Díaz-Uriarte R, Dopazo J. FatiGO: a web tool for finding significant associations of Gene Ontology terms with groups of genes. Bioinformatics. 2004;20:578–80.PubMedView ArticleGoogle Scholar
- Medina I, Carbonell J, Pulido L, Madeira SC, Goetz S, Conesa A, Tarraga J, Pascual-Montano A, Nogales-Cadenas R, Santoyo J, et al. Babelomics: an integrative platform for the analysis of transcriptomics, proteomics and genomic data with advanced functional profiling. Nucleic Acids Res. 2010;38:W210–3.PubMedPubMed CentralView ArticleGoogle Scholar
- Prufer K, Racimo F, Patterson N, Jay F, Sankararaman S, Sawyer S, Heinze A, Renaud G, Sudmant PH, de Filippo C, et al. The complete genome sequence of a Neanderthal from the Altai Mountains. Nature. 2014;505:43–9.PubMedView ArticleGoogle Scholar
- Dobrynin P, Liu S, Tamazian G, Xiong Z, Yurchenko A, Krasheninnikova K, Kliver S, Schmidt-Kuntzel A, Koepfli K-P, Johnson W, et al. Genomic legacy of the African cheetah, Acinonyx jubatus. Genome Biol. 2015;16:277.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhou XM, Sun FM, Xu SX, Fan GY, Zhu KL, Liu X, Chen Y, Shi CC, Yang YX, Huang ZY, et al. Baiji genomes reveal low genetic variability and new insights into secondary aquatic adaptations. Nat Commun. 2013;4:2708.Google Scholar
- Carbone L, Alan Harris R, Gnerre S, Veeramah KR, Lorente-Galdos B, Huddleston J, Meyer TJ, Herrero J, Roos C, Aken B, et al. Gibbon genome and the fast karyotype evolution of small apes. Nature. 2014;513:195–201.PubMedPubMed CentralView ArticleGoogle Scholar
- Yim HS, Cho YS, Guang XM, Kang SG, Jeong JY, Cha SS, Oh HM, Lee JH, Yang EC, Kwon KK, et al. Minke whale genome and aquatic adaptation in cetaceans. Nat Genet. 2014;46:88–92.PubMedView ArticleGoogle Scholar
- Wu HG, Guang XM, Al-Fageeh MB, Cao JW, Pan SK, Zhou HM, Zhang L, Abutarboush MH, Xing YP, Xie ZY, et al. Camelid genomes reveal evolution and adaptation to desert environments. Nat Commun. 2014;5:5188.Google Scholar
- Palkopoulou E, Mallick S, Skoglund P, Enk J, Rohland N, Li H, Omrak A, Vartanyan S, Poinar H, Götherström A, et al. Complete genomes reveal signatures of demographic and genetic declines in the woolly mammoth. Curr Biol. 2015;25:1395–400.PubMedPubMed CentralView ArticleGoogle Scholar
- Fang XD, Nevo E, Han LJ, Levanon EY, Zhao J, Avivi A, Larkin D, Jiang XT, Feranchuk S, Zhu YB, et al. Genome-wide adaptive complexes to underground stresses in blind mole rats Spalax. Nat Commun. 2014;5:3966.Google Scholar