DNA methylation age of human tissues and cell types
© Horvath; licensee BioMed Central Ltd. 2013
Received: 10 June 2013
Accepted: 4 October 2013
Published: 10 December 2013
The Erratum to this article has been published in Genome Biology 2015 16:96
It is not yet known whether DNA methylation levels can be used to accurately predict age across a broad spectrum of human tissues and cell types, nor whether the resulting age prediction is a biologically meaningful measure.
I developed a multi-tissue predictor of age that allows one to estimate the DNA methylation age of most tissues and cell types. The predictor, which is freely available, was developed using 8,000 samples from 82 Illumina DNA methylation array datasets, encompassing 51 healthy tissues and cell types. I found that DNA methylation age has the following properties: first, it is close to zero for embryonic and induced pluripotent stem cells; second, it correlates with cell passage number; third, it gives rise to a highly heritable measure of age acceleration; and, fourth, it is applicable to chimpanzee tissues. Analysis of 6,000 cancer samples from 32 datasets showed that all of the considered 20 cancer types exhibit significant age acceleration, with an average of 36 years. Low age-acceleration of cancer tissue is associated with a high number of somatic mutations and TP53 mutations, while mutations in steroid receptors greatly accelerate DNA methylation age in breast cancer. Finally, I characterize the 353 CpG sites that together form an aging clock in terms of chromatin states and tissue variance.
I propose that DNA methylation age measures the cumulative effect of an epigenetic maintenance system. This novel epigenetic clock can be used to address a host of questions in developmental biology, cancer and aging research.
An increasing body of evidence suggests that many manifestations of aging are epigenetic [1, 2]. This article focuses on one particular type of epigenetic control: cytosine-5 methylation within CpG dinucleotides (also known as DNA methylation). Age-related DNA hypomethylation has long been observed in a variety of species, including salmon , rats , and mice . More recent studies have shown that many CpGs are subject to age-related hypermethylation. A vast literature characterizes genes or genomic regions that either get hypermethylated or hypomethylated with age [6–14]. Previous studies have shown that age-related hypermethylation occurs preferentially at CpG islands , at bivalent chromatin domain promoters that are associated with key developmental genes , and at Polycomb-group protein targets . The epigenomic landscape varies markedly across tissue types [16–18] and many age-related changes depend on tissue type [8, 19]. But several recent studies have shown that age-dependent CpG signatures can be defined independently of sex, tissue type, disease state and array platform [10, 13–15, 20–22]. While several recent articles describe age predictors based on DNAm levels in specific tissues (for example, saliva or blood [23, 24]), it is not yet known whether age can be predicted irrespective of tissue type using a single predictor. Here I use an unprecedented collection of publicly available DNA methylation data sets for defining and evaluating an age predictor. Its astonishing accuracy across most tissues and cell types justifies its designation as a multi-tissue age predictor. Its age prediction, referred to as DNAm age, can be used as a biomarker for addressing a host of questions arising in aging research and related fields. For example, I show that premature aging diseases (such as progeria) do not resemble healthy normal aging according to DNAm age and that interventions used for creating induced pluripotent stem (iPS) cells reset the epigenetic clock to zero. I also describe what can be learnt from applying DNAm age to cancer tissues and cancer cell lines.
Results and discussion
Description of the (non-cancer) DNA methylation data sets
I assembled a large DNA methylation data set by combining publicly available individual data sets measured on the Illumina 27K or Illumina 450K array platform. In total, I analyzed n = 7,844 non-cancer samples from 82 individual data sets (Additional file 1), which assess DNA methylation levels in 51 different tissues and cell types. Although many data sets were collected for studying certain diseases (Additional file 2), they largely involved healthy tissues. In particular, cancer tissues were excluded from this first large data set since it is well known that cancer has a profound effect on DNA methylation levels [6, 7, 24–26]. The Cancer Genome Atlas (TCGA) data sets mentioned in Additional file 1 involved normal adjacent tissue from cancer patients. Details on the individual data sets and data pre-processing steps are provided in Materials and methods and Additional file 2. As described in Additional file 1, the first 39 data sets were used to construct (‘train’) the age predictor. Data sets 40 to 71 were used to test (validate) the age predictor. Data sets 72 to 82 served other purposes - for example, to estimate the DNAm age of embryonic stem and iPS cells. The criteria used for selecting the training sets are described in Additional file 2. Briefly, the training data were chosen i) to represent a wide spectrum of tissues/cell types, ii) to involve samples whose mean age (43 years) is similar to that in the test data, and iii) to involve a high proportion of samples (37%) measured on the Illumina 450K platform since many on-going studies use this recent Illumina platform. Here I only studied 21,369 CpGs (measured with the Infinium type II assay) that were present on both Illumina platforms (Infinium 450K and 27K) and had fewer than 10 missing values across the data sets. Several important limitations of this study are discussed in Additional file 2.
The multi-tissue age predictor used for defining DNAm age
To ensure an unbiased validation in the test data, I only used the training data to define the age predictor. As detailed in Materials and methods and Additional file 2, a transformed version of chronological age was regressed on the CpGs using a penalized regression model (elastic net). The elastic net regression model automatically selected 353 CpGs (Additional file 3). I refer to the 353 CpGs as (epigenetic) clock CpGs since their weighted average (formed by the regression coefficients) amounts to an epigenetic clock. Before characterizing them, I will show that the resulting age predictor performs remarkably well across a wide spectrum of tissues and cell types.
Predictive accuracy across different tissues
I considered several measures of predictive accuracy since each measure has distinct advantages. The first, referred to as ‘age correlation’, is the Pearson correlation coefficient between DNAm age (predicted age) and chronological age. It has the following limitations: it cannot be used for studying whether DNAm is well calibrated, it cannot be calculated in data sets whose subjects have the same chronological age (for example, cord blood samples from newborns), and it strongly depends on the standard deviation of age (as described below). The second accuracy measure, referred to as (median) ‘error’, is the median absolute difference between DNAm age and chronological age. Thus, a test set error of 3.6 years indicates that DNAm age differs by less than 3.6 years in 50% of subjects. The error is well suited for studying whether DNAm age is poorly calibrated. ‘Average age acceleration’, defined by the average difference between DNAm age and chronological age, can be used to determine whether the DNAm age of a given tissue is consistently higher (or lower) than expected.
The age predictor is particularly accurate in data sets composed of adolescents and children - for example, blood (Figures 1B and 2B; Additional file 4P,S), brain data (Figures 1F and 2F,G), and buccal epithelium (Figure 2I).
The DNAm age of blood and brain cells
A detailed analysis of blood tissue can be found in Additional file 4. Human blood cells have different life spans: while CD14+ monocytes (myeloid lineage) only live several weeks, CD4+ T cells (lymphoid lineage) represent a variety of cell types that can live from months to years. An interesting question is whether blood cell types have different DNAm ages. DNAm age does not vary significantly across sorted blood cells from healthy male subjects (Additional file 4T). These results combined with the fact that the age predictor works well in individual cell types (Figure 2C; Additional file 4) strongly suggest that DNAm age does not reflect changes in cell type composition but rather intrinsic changes in the methylome. While I expect significant correlations between DNAm age and abundance measures of some blood cell types (that are known to change with age), these correlations do not reflect a direct causal effect of cell type abundance on DNAm age but rather a confounding effect due to chronological age. This conclusion is also corroborated by the finding that DNAm age is highly related to chronological age in other types of cells - for example, glial cells and neurons (Figure 1H) and various brain regions (Additional file 5).
DNAm age and progeria
DNAm age can be used to study whether cells from patients with accelerated aging diseases such as progeria (including Werner progeroid syndrome, Hutchinson-Gilford progeria) truly look old at an epigenetic level. I find that progeria disease status is not related to DNAm-based age acceleration in Epstein-Barr virus-transformed B cells (Figure 2T).
Tissues where DNAm age is poorly calibrated
DNAm age is poorly calibrated (that is, leads to a high error) in breast tissue (Figure 2H), uterine endometrium (Figure 2S), dermal fibroblasts (Figure 1M), skeletal muscle tissue (Figure 2P), and heart tissue (Figures 1Q and 2L). I can only speculate on the biological reasons that could explain the poor calibration. The high error in breast tissue (Additional file 6) may reflect hormonal effects or cancer field effects in this normal adjacent tissue from cancer samples. Note that the lowest error (8.9 years) in breast tissue is observed in normal breast tissue, that is, in samples from women without cancer (training data set 14; Additional file 6). The menstrual cycle and concomitant increases in cell proliferation may explain the high error in uterine endometrium. Myosatellite cells may effectively rejuvenate the DNAm age of skeletal muscle tissue. Similarly, the recruitment of stem cells into cardiomyocytes for new cardiac muscle formation could explain why human heart tissue tends to have a low DNAm age. Carefully designed studies will be needed to test these hypotheses.
The age correlation in a data set is determined by the standard deviation of age
In the following, I describe non-biological reasons that affect the accuracy (age correlation) of the age predictor. To address how well the age predictor works in individual data sets, I used two different approaches. First, I applied the age predictor to individual data sets (see columns ‘Cor(Age, DNAmAge)’ and ‘Median Error(Age, DNAmAge)’ in Additional file 1). An obvious limitation of this approach is that it leads to biased results in the training data sets.
The second approach, referred to as leave-one-data-set-out cross-validation (LOOCV) analysis, leads to unbiased estimates of the predictive accuracy for each data set. As suggested by its name, this approach estimates the DNAm age for each data set (considered as test data set) separately by fitting a separate multi-tissue age predictor to the remaining (left out) data sets. The resulting unbiased estimates of predictive accuracy can be found in Additional file 1: columns ‘Cor LOOCV’ and ‘Error LOOCV’, respectively.
A host of technical artifacts could explain differences in predictive accuracy: for example, variations in sample processing, DNA extraction, DNA storage effects, batch effects, and chip effects.
DNAm age of multiple tissues from the same subject
In the following, I will address whether solid tissues can be found whose DNAm age differs substantially from chronological age. As a first step, I compared the mean DNAm age per tissue with the corresponding mean chronological age. As expected, mean DNAm age per tissue is highly correlated (cor = 0.99; Figure 3C) with mean chronological age. But breast tissue shows evidence of significant age acceleration. The results of Figure 3C should be interpreted with caution because the analysis included training data sets and involved tissue samples from different subjects.
A more interesting analysis is to compare the DNAm ages of tissues collected from the same subjects. DNAm age does not change significantly across different brain regions (temporal cortex, pons, frontal cortex, cerebellum) from the same subjects (Additional file 5K,L). I could only find three human subjects from whom many tissues had been profiled (Figure 3E-G). Although the limited sample sizes per tissue (mostly one sample per tissue per subject) did not allow for rigorous testing, these data can be used to estimate the coefficient of variation of DNAm age (that is, the standard deviation divided by the mean). Note that the coefficient of variations for the first and second adult male are relatively low (0.12 and 0.15 in Figure 3E,F) even though the analysis involved several tissues that were not part of the training data - for example, jejunum, penis, pancreas, esophagus, spleen, pancreas, lymph node, diaphragm. The coefficient of variation in the adult female (Figure 3G) is relatively high (0.21), which reflects the fact that her breast tissue shows signs of substantial age acceleration (congruent with the previous results from Figure 3C).
It remains to be seen how well DNAm age performs in tissues and DNA sources that were not represented in the training data set. Figure 3D,E suggest that it also performs well in several other human tissues. As expected, I did not find a significant age correlation in sperm. The DNAm age of sperm is significantly lower than the chronological age of the donor (Figure 3I, J).
DNAm age is applicable to chimpanzees
DNAm age of induced pluripotent stem cells and stem cells
Effect of cell passaging on DNAm age
Most cells lose their proliferation and differentiation potential after a limited number of cell divisions (Hayflick limit). I hypothesized that cell passaging (also known as splitting cells) increases DNAm age. I confirmed this hypothesis in three independent data sets (data sets 77, 78, and 79) as shown in Figure 5F-J. A significant correlation between cell passage number and DNAm age can also be observed when restricting the analysis to iPS cells (Figure 5G) or when restricting the analysis to ES cells (Figure 5H,J).
Comparing the multi-tissue predictor with other age predictors
As shown in Additional file 2, the proposed multi-tissue predictor of age outperforms predictors described in other articles [21, 23]. While further gains in accuracy can perhaps be achieved by focusing on a single tissue and considering more CpGs, the major strength of the proposed multi-tissue age predictor lies in its wide applicability: for most tissues it will not require any adjustments or offsets. I briefly mention that a ‘shrunken’ version of the multi-tissue predictor (Additional files 2 and 3), based on 110 CpGs (selected from the 353 clock CpGs) is highly accurate in the training data (cor = 0.95, error = 4 years) and test data (cor = 0.95, error = 4.2 years).
What is known about the 353 clock CpGs?
An Ingenuity Pathway analysis of the genes that co-locate with the 353 clock CpGs shows significant enrichment for cell death/survival, cellular growth/proliferation, organismal/tissue development, and cancer (Additional file 7).
The 353 clock CpGs can be divided into two sets according to their correlation with age. The 193 positively and 160 negatively correlated CpGs get hypermethylated and hypomethylated with age, respectively. Using DNA methylation data measured across many different adult and fetal tissues, I study the relationship between tissue variance and age effects (Additional file 8). While the DNA methylation levels of the 193 positively related CpGs vary less across different tissues, those of the 160 negatively related CpGs vary more across tissues than the remaining CpGs on the Illumina 27K array. To estimate ‘pure’ age effects, I used a meta analysis method that implicitly conditions on data set, that is, it removes the confounding effects due to data set and tissue type. The clock CpGs include those with the most significant meta analysis P-value for age irrespective of whether the meta analysis P-value was calculated using only training data sets or all data sets (Additional file 8E). While positively related markers do not show a significant relationship with CpG island status (Additional file 9F), negatively related markers tend to be over-represented in CpG shores (P = 9.3E-6; Additional file 9K).
Significant differences between positive and negative markers exist when it comes to Polycomb-group protein binding: positively related CpGs are over-represented near Polycomb-group target genes (reflecting results from [10, 14]) while negative CpGs show no significant relationship (Additional file 9H-J,M-O).
Chromatin state analysis
Chromatin state profiling has emerged as a powerful means of genome annotation and detection of regulatory activity. It provides a systematic means of detecting cis-regulatory elements (given the central role of chromatin in mediating regulatory signals and controlling DNA access) and can be used for characterizing non-coding portions of the genome, which contribute to cellular phenotypes . While individual histone modifications are associated with regulator binding, transcriptional initiation, enhancer activity, combinations of chromatin modifications can provide even more precise insight into chromatin state . Ernst et al.  distinguish six broad classes of chromatin states, referred to as promoter, enhancer, insulator, transcribed, repressed, and inactive states. Within them, active, weak and poised promoters (states 1 to 3) differ in expression levels, while strong and weak enhancers (states 4 to 7) differ in expression of proximal genes. The 193 positively related CpGs are more likely to be in poised promoters (chromatin state 3 regions; Additional file 9B) while the 160 negatively related CpGs are more likely to be in either weak promoters (chromatin state 2; Additional file 9D) or strong enhancers (chromatin state 4; Additional file 9E).
Age acceleration is highly heritable
Several authors have found that DNA methylation levels are under genetic control [24, 26, 30–32]. Since many age-related diseases are heritable, it is interesting to study whether age acceleration (here defined as difference between DNAm age and chronological age) is heritable as well. I estimated the broad sense heritability of age acceleration using Falconer’s formula, H2 = 2(cor(MZ)-cor(DZ)), in two twin data sets that included both monozygotic (MZ) and dizygotic (DZ) twins.
As detailed in Additional file 10, the broad sense heritability of age acceleration is 100% in newborns (data set 50) and 39% in older subjects (data set 41), which suggests that non-genetic factors become more relevant later in life.
Aging effects on gene expression (messenger RNA) levels
Since DNA methylation is an important epigenetic mechanism for regulating gene expression levels (messenger RNA abundance), it is natural to wonder how age-related DNAm changes relate to those observed in gene expression levels. As described in Additional file 11, I find very little overlap. Further, I do not find that age effects on DNAm levels affect genes known to be differentially expressed between naive CD8 T cells and CD8 memory cells (Additional file 11). These non-significant results reflect the fact that the relationship between DNAm levels and expression levels is complex [33, 34].
Age effects on individual CpGs
The changing ticking rate of the epigenetic clock
The linear combination of the 353 clock CpGs (resulting from the regression coefficients) varies greatly across ages as can be seen from Figure 6B,C. The red calibration curve (formula in Additional file 2) reveals a logarithmic dependence until adulthood that slows to a linear dependence later in life (Figure 6B). I interpret the rate of change (of this red curve) as the ticking rate of the epigenetic clock. Using this terminology, I find that organismal growth (and concomitant cell division) leads to a high ticking rate that slows down to a constant ticking rate (linear dependence) after adulthood.
DNAm age does not measure mitotic age or cellular senescence
Since epigenetic somatic errors in somatic replications appear to be readily detected as age-related changes in methylation [35, 36], it is a plausible hypothesis that DNAm age measures the number of somatic cell replications. In other words, that it measures mitotic age (which assigns a cell copy number to every cell) [35, 37]. While DNAm age is correlated with cell passage number (Figure 5) and the clock ticking rate is highest during organismal growth (Figure 6B,C), it is clearly different from mitotic age since it tracks chronological age in non-proliferative tissue (for example, brain tissue) and assigns similar ages to both short and long lived blood cells (Additional file 4T).
Another plausible hypothesis is that DNAm age is a marker of cellular senescence. This turns out to be wrong as can be seen from the fact that DNAm age is highly related to chronological age in immortal, non-senescent cells - for example, immortalized B cells (Figure 2T). Further, DNAm age and cell passage number are highly correlated in ES cells (Figure 5H,J), which are also immortal .
Model: DNAm age measures the work done by an epigenetic maintenance system
I propose that DNAm age measures the cumulative work done by a particular kind of epigenetic maintenance system (EMS), which helps maintain epigenetic stability. While epigenetic stability is related to genomic stability, I find it useful to distinguish these two concepts. If the EMS model of DNAm age is correct, then this particular kind of EMS appears to be inactive in the perfectly young ES cells. Maintenance methyltransferases are likely to play an important role. In physics, ‘work’ is defined by the integral of power over time. Using this terminology, I hypothesize that the power (defined as rate of change of the energy spent by this EMS) corresponds to the tick rate of the epigenetic clock. This model would explain the high tick rate during organismal development since a high power is required to maintain epigenetic stability during this stressful time. At the end of development, a constant amount of power is sufficient to maintain stability leading to a constant tick rate.
If this EMS model of DNAm age is correct, then DNAm age should be accelerated by many perturbations that affect epigenetic stability. Further, age acceleration should have some beneficial effects given the protective role of the EMS. In particular, the EMS model of DNAm age entails the following testable predictions. First, cancer tissue should show signs of accelerated age, reflecting the protective actions of the EMS. Second, many mitogens, genomic aberrations, and oncogenes, which trigger the response of the EMS, should be associated with accelerated DNAm age. Third, high age acceleration of cancer tissue should be associated with fewer somatic mutations given the protective role of the EMS. Fourth, mutations in TP53 should be associated with a lower age acceleration of cancer tissue if one further assumes that p53 signaling helps trigger the EMS.
All of these model predictions turn out to be true as will be shown in the following cancer applications.
DNAm age of cancer tissue versus tumor morphology
I assembled a large collection of cancer data sets composed of n = 5,826 cancer samples from 32 individual cancer data sets (Additional file 12). Details on the cancer data sets can be found in Additional file 2. While some cancer tissues show relatively large correlations between DNAm age and patient age, the correlation between DNAm age and chronological age tends to be weak (cor = 0.15, P = 1.9E-29; Additional file 13A). Each cancer/affected tissue shows evidence of significant age acceleration with an average age acceleration of 36.2 years (Additional file 13B). Tumor morphology (grade and stage) has only a weak relationship with age acceleration in most cancers: only 4 out of 33 hypothesis tests led to a nominally (P < 0.05) significant result (Additional file 14). Only the negative correlation between stage and age acceleration in thyroid cancer remains significant (uncorrected P = 8.7E-9; Additional file 14Z) after applying a Bonferroni correction.
Cancer tissues with high age acceleration exhibit fewer somatic mutations
TP53 mutations are associated with lower age acceleration
Additional file 16 presents the genes whose mutation has the strongest effect on age acceleration. Strikingly, TP53 was among the top 2 most significant genes in 4 out of the 13 cancer data sets. Further, TP53 mutation is associated with significantly lower age acceleration in five different cancer types (Additional file 17), including AML (P = 0.0023), breast cancer (P = 1.4E-5 and P = 3.7E-8), ovarian cancer (P = 0.03), and uterine corpus endometrioid (P = 0.00093). Further, marginally significant result can be observed in lung squamous cell carcinoma (Additional file 17) and colorectal cancer (P = 0.073, below). I could only find one cancer type (glioblastoma multiforme (GBM)) where mutations in TP53 are associated with a nominally significant increased age acceleration (P = 0.02; Figure 5H). Overall, these results suggest that p53 signaling can trigger processes that accelerate DNAm age, which supports the EMS model of DNAm age.
Somatic mutations in steroid receptors accelerate DNAm age in breast cancer
Proto-oncogenes affect DNAm age in colorectal cancer
DNAm age in glioblastoma multiforme
In general, the CpG island methylator phenotype and age acceleration measure different properties as can be seen in GBM (Figure 9M).
Interestingly, age acceleration in GBM samples is highly significantly (P = 3.3E-7; Figure 9J) associated with certain mutations in H3F3A, which encodes the replication-independent histone variant H3.3. These mutations are single-nucleotide variants changing lysine 27 to methionine (K27M) or changing glycine 34 to arginine (G34R) . The fact that GBMs with a G34R mutation in H3F3A have a much higher age acceleration than those with a K27M mutation (Figure 9J,L) makes sense since each H3F3A mutation defines an epigenetic subgroup of GBM with a distinct global methylation pattern and acts through a different set of genes . Lysine 27 is a critical residue of histone 3 variants, and methylation at this position (H3K27me), which may be mimicked by the terminal CH3 of methionine substituted at this residue , is commonly associated with transcriptional repression  while H3K36 methylation or acetylation typically promotes gene transcription . G34-mutant cells exhibit increased RNA polymerase II binding, and increased gene expression, most notably that of the oncogene MYCN. Both H3F3A mutations are mutually exclusive with IDH1 mutations, which characterize a third mutation-defined subgroup . Age acceleration in GBM samples is also associated with the following genomic aberrations: TP53 mutation, ATRX mutation, chromosome 7 gain, chromosome 10 loss, CDKN2A deletion, and EGFR amplification (Figure 9G-I). Reflecting these results for individual markers, age acceleration varies significantly (P = 2E-7; Figure 9L) across the GBM subtypes defined in .
Acute myeloid leukemia
Mutations in IDH1 (similar to the case of GBM), FLT, RAS, NPMc, and various well characterized translocations do not seem to relate to age acceleration in AML samples (Figure 9S-W).
DNAm age of cancer cell lines
Using seven publicly available cell line data sets (Additional files 12 and 13), I was able to estimate the DNAm age of 59 different cancer cell lines (from bladder, breast, gliomas, head/neck, leukemia, and osteosarcoma). Across all cell lines, DNAm age does not have a significant correlation with the chronological age of the patient from whom the cancer cell line was derived (Additional file 18B). However, a marginally significant age correlation can be observed across osteosarcoma cell lines (cor = 0.41, P = 0.08; Additional file 18C). Overall, DNAm age acceleration varies greatly across the cancer lines lines (Additional files 18A and 19): the highest values can be observed for AML cell lines (KG1A, 182 years; HL-60, 177 years); the lowest values for head/neck squamous cell carcinoma cell line (UPCI SCC47, 6 years) and two breast cancer cell lines (SK-BR-3, 8 years; MDA-MB-468, 11 years). It will be interesting to test whether DNAm age relates to other characteristics of cancer cell lines.
Through the generosity of hundreds of researchers, I was able to analyze an unprecedented collection of DNA methylation data from healthy tissues, cancer tissues, and cancer cell lines. The healthy tissue data allowed me to develop a multi-tissue predictor of age (mathematical details are provided in Additional file 2). An R software tutorial can be found in Additional file 20 (which requires Additional files 21, 22, 23, 24, 25, 26 and 27 as input). The basic approach is to form a weighted average of the 353 clock CpGs, which is then transformed to DNAm age using a calibration function. The calibration function reveals that the epigenetic clock has a high tick rate until adulthood, after which it slows to a constant tick rate.
I propose that DNAm age measures the cumulative work done by an epigenetic maintenance system. This EMS model of DNAm age leads to several testable model predictions that I validate using cancer data. But irrespective of the validity of the EMS model, the findings in cancer are interesting in their own right. While all cancer tissues exhibit signs of severe age acceleration, this is not necessarily the case for individual cancer cell lines. Overall, high age acceleration is associated with fewer somatic mutations in cancer tissue. Mutations in TP53 are associated with lower DNAm age. To provide a glimpse of how DNAm age can inform cancer research, I relate it to several widely used genomic aberrations in breast cancer, colorectal cancer, GBM, and AML.
DNAm age is arguably a promising marker for studying human development, aging, and cancer. It may become a useful surrogate marker for evaluating rejuvenation therapies. The most salient feature of DNAm age is its applicability to a broad spectrum of tissues and cell types. Since it allows one to contrast the ages of different tissues from the same subject, it can be used to identify tissues that show evidence of accelerated age due to disease (for example, cancer). It remains to be seen whether the DNAm age of easily accessible fluids/tissues (for example, saliva, buccal cells, blood, skin) can serve as a surrogate marker for inaccessible tissues (for example, brain, kidney, liver). It is noteworthy that DNAm age is applicable to chimpanzee tissues. Given the high heritability of age acceleration in young subjects, I expect that age acceleration will mainly be a relevant measure in older subjects. Using a relatively small data set, I did not find any evidence that a premature aging disease (progeria) is associated with accelerated DNAm age (Figure 2T). In Additional file 2, I discuss whether DNAm age fulfils the biomarker criteria developed by the American Federation for Aging Research.
Future research will need to clarify whether DNAm age is only a marker of aging or relates to an effector of aging. In conclusion, the epigenetic clock described here is likely to become a valuable addition to the telomere clock.
Materials and methods
Definition of DNAm age using a penalized regression model
Using the training data sets, I used a penalized regression model (implemented in the R package glmnet ) to regress a calibrated version of chronological age on 21,369 CpG probes that a) were present both on the Illumina 450K and 27K platform and b) had fewer than 10 missing values. The alpha parameter of glmnet was chosen to 0.5 (elastic net regression) and the lambda value was chosen using cross-validation on the training data (lambda = 0.0226). DNAm age was defined as predicted age. Mathematical details are provided in Additional file 2.
Short description of the healthy tissue data sets
All data are publicly available (Additional file 1). Many data sets involve normal adjacent tissue from TCGA. Details on the individual data sets can be found in Additional file 2. To give credit to the many researchers who generated the data, I briefly mention relevant citations. Data sets 1 and 2 (whole blood samples from a Dutch population) were generated by Roel Ophoff and colleagues . Data set 3 (whole blood) consists of whole blood samples from a recent large scale study of healthy individuals . The authors used these and other data to estimate human aging rates and developed a highly accurate predictor of age based on blood data. Data set 4 consists of leukocyte samples from healthy male children from Children’s Hospital Boston . Data set 5 consists of peripheral blood leukocyte samples . Data set 6 consists of cord blood samples from newborns . Data set 7 consists of cerebellum samples, which were provided by C Liu and C Chen (Gene Expression Omnibus (GEO) identifier GSE38873). Data sets 8, 9, 10, and 13 consist of cerebellum, frontal cortex, pons, and temporal cortex samples, respectively, obtained from the same subjects . Data set 11 consists of prefrontal cortex samples from healthy controls . Data set 12 consists of neuron and glial cell samples from . Data set 14 consists of normal breast tissue samples . Data set 15 consists of buccal cells from 109 15-year-old adolescents from a longitudinal study of child development . Data set 16 consists of buccal cells from eight different subjects . Data set 17 consists of buccal cells from monozygotic (MZ) and dizygotic (DZ) twin pairs from the Peri/postnatal Epigenetic Twins Study (PETS) cohort . Data set 18 consists of cartilage (chondrocyte) samples from . Data set 19 normal consists of adjacent colon tissue from TCGA. Data set 20 consists of colon mucosa samples from . Data set 21 consists of dermal fibroblast samples from . Data set 22 consists of epidermis samples from . Data set 23 consists of gastric tissue samples from . Data set 24 consists of head/neck normal adjacent tissue samples from TCGA (HNSC data). Data set 25 consists of heart tissue samples from . Data set 26 consists of normal adjacent renal papillary tissue from TCGA (KIRP data). Data sets 27 consists of normal adjacent tissue from TCGA (KIRC data). Data set 28 consists of normal adjacent liver samples from . Data set 29 consists of normal adjacent lung tissue from TCGA (LUSC data). Data set 30 consists of normal adjacent lung tissue samples from TCGA (LUAD data). Data set 31 is from TCGA (LUSC). Data set 32 consists of mesenchymal stromal cells isolated from bone marrow . Data set 33 consists of placenta samples from mothers of monozygotic and dizygotic twins . Data set 34 consists of prostate samples from . Data set 35 consists of normal adjacent prostate tissue from TCGA (PRAD data). Data set 36 consists of male saliva samples from . Data set 37 consists of male saliva samples from . Data set 38 consists of stomach from TCGA (STAD data). Data set 39 consists of thyroid TCGA (THCA data). Data set 40 consists of whole blood from type 1 diabetics [10, 63]. Data set 41 consists of whole blood from . Data sets 42 and 43 consist of involve whole blood samples from women with ovarian cancer and healthy controls, respectively; these are the samples from the United Kingdom Ovarian Cancer Population Study [10, 63]. Data set 44 consists of whole blood from . Data set 45 consists of leukocytes from healthy children of the Simons Simple Collection . Data set 46 consists of peripheral blood mononuclear cells from . Data set 47 consists of peripheral blood mononuclear cells from . Data set 48 consists of cord blood samples from newborns provided by N Turan and C Sapienza (GEO GSE36812). Data set 49 consists of cord blood mononuclear cells from . Data set 50 consists of cord blood mononuclear cells from . Data set 51 consists of CD4 T cells from infants . Data set 52 consists of CD4+ T cells and CD14+ monocytes from . Data set 53 consists of immortalized B cells and other cells from progeria, Werner syndrome patients, and controls . Data sets 54 and 55 are brain samples from . Data sets 56 and 57 consist of breast tissue from TCGA (27K and 450K platforms, respectively). Data set 58 consists of buccal cells from . Data set 59 consists of colon from TCGA (COAD data). Data set 60 consists of fat (adipose) tissue from . Data set 61 consists of human heart tissue from . Data set 62 consists of kidney (normal adjacent) tissue from TCGA (KIRC). Data set 63 consists of liver (normal adjacent tissue) from TCGA (LIHC data). Data set 64 consists of lung from TCGA. Data set 65 consists of muscle tissue from . Data set 66 consists of muscle tissue from . Data set 67 consists of placenta samples from . Data set 68 consists of female saliva samples . Data set 69 consists of uterine cervix samples from [50, 75]. Data set 70 consists of uterine endometrium (normal adjacent) tissue from TCGA (UCEC data). Data set 71 consists of various human tissues from the ENCODE/HAIB Project (GEO GSE40700). Data set 72 consists of chimpanzee and human tissues from . Data set 73 consists of great ape blood samples from . Data set 74 consists of sperm samples from . Data set 75 consists of sperm samples from . Data set 76 consists of vascular endothelial cells from human umbilical cords from . Data sets 77 and 78 (special cell types) involve human embryonic stem cells, iPS cells, and somatic cell samples measured on the Illumina 27K array and Illumina 450K array, respectively . Data set 79 consists of reprogrammed mesenchymal stromal cells from human bone marrow (iP-MSC), initial mesenchymal stromal cells, and embryonic stem cells . Data set 80 consists of human ES cells and normal primary tissue from . Data set 81 consists of human ES cells from . Data set 82 consists of blood cell type data from .
Description of the cancer data sets
All data are publicly available as can be seen from the column that reports GSE identifiers from the GEO database and other online resources. Most cancer data sets came from TCGA. Data set 3, GBM from ; data set 4, breast cancer from ; data set 5, breast cancer from ; data set 6, breast cancer from ; data set 10, colorectal cancer from ; data set 23, prostate cancer from ; data set 30, urothelial carcinoma from .
DNA methylation profiling and normalization steps
All of the public Illumina DNA data were generated by following the standard protocol of Illumina methylation assays, which quantifies DNA methylation levels by the β value. A detailed description of the pre-processing and data normalization steps is provided in Additional file 2.
Meta analysis for measuring pure age effects (irrespective of tissue type)
Analysis of variance for measuring tissue variation
To measure tissue effects in the training data (Additional file 8), I used analysis of variance (ANOVA) to calculate an F statistic as follows. First, a multivariate regression model was used to regress each CpG (dependent variable) on age and tissue type. The analysis adjusted for age since the different data sets have very different mean ages (Additional file 1). Next, ANOVA based on the multivariate regression model was used to calculate an F statistic, F.tissueTraining, for measuring the tissue effect in the training data. This F statistic measures the tissue effect after adjusting for age in the training data sets. I did not translate the F statistic into a corresponding P-value since the latter turned out to be extremely significant for most CpGs. Additional file 8D shows that F.tissueTraining is highly correlated with an independent measure of tissue variance (defined using adult somatic tissues from data set 77).
Characterizing the CpGs using sequence properties
I studied occupancy counts for Polycomb-group target (PCGT) genes since they have an increased chance of becoming methylated with age compared to non-targets . Toward this end, I used the occupancy counts of Suz12, Eed, and H3K27me3 published in . To obtain the protein binding site occupancy throughout the entire nonrepeat portion of the human genome, Lee et al.  isolated DNA sequences bound to a particular protein of interest (for example, Polycomb-group protein SUZ12) by immunoprecipitating that protein (chromatin immunoprecipitation) and subsequently hybridizing the resulting fragments to a DNA microarray. More details on the chromatin state data from  can be found in Additional file 2.
Acute myeloid leukemia
Bladder urothelial carcinoma
Cord blood mononuclear cell
Cervical squamous cell carcinoma and endocervical adenocarcinoma
Epigenetic maintenance system
Gene Expression Omnibus
Head/neck squamous cell carcinoma
Cell- human umbilical vascular endothelial cells
Induced pluripotent stem
Kidney renal clear cell carcinoma
Kidney renal papillary cell carcinoma
Liver hepatocellular carcinoma
Leave one data set out
Mesenchymal stromal cell
Skin cutaneous melanoma
The Cancer Genome Atlas
Uterine corpus endometrioid carcinoma.
I gratefully acknowledge the many researchers who made their DNA-methylation datasets publicly available and responded to my email requests. This study would not have been possible without the valuable data from The Cancer Genome Atlas. I gratefully acknowledge discussions with Prof. Darryl Shibata from the University of Southern California about mitotic age. No specific funding was received for this work.
- Oberdoerffer P, Sinclair DA: The role of nuclear architecture in genomic instability and ageing. Nat Rev Mol Cell Biol 2007, 8:692–702.View ArticlePubMedGoogle Scholar
- Campisi J, Vijg J: Does damage to DNA and other macromolecules play a role in aging? If so, how? J Gerontol A Biol Sci Med Sci 2009, 64A:175–178.View ArticlePubMed CentralGoogle Scholar
- Berdyshev G, Korotaev G, Boiarskikh G, Vaniushin B: Nucleotide composition of DNA and RNA from somatic tissues of humpback and its changes during spawning. Biokhimiia 1967, 31:88–993.Google Scholar
- Vanyushin B, Nemirovsky L, Klimenko V, Vasiliev V, Belozersky A: The 5 mehylcytosine in DNA of rats. Tissue and age specificity and the changes induced by hydrocortisone and other agents. Gerontologia 1973, 19:138–152.View ArticlePubMedGoogle Scholar
- Wilson V, Smith R, Ma S, Cutler R: Genomic 5-methyldeoxycytidine decreases with age. J Biol Chem 1987, 262:9948–9951.PubMedGoogle Scholar
- Fraga MF, Agrelo R, Esteller M: Cross-talk between aging and cancer. Ann N Y Acad Sci 2007, 1100:60–74.View ArticlePubMedGoogle Scholar
- Fraga MF, Esteller M: Epigenetics and aging: the targets and the marks. Trends Genet 2007, 23:413–418.View ArticlePubMedGoogle Scholar
- Christensen BC, Houseman EA, Marsit CJ, Zheng S, Wrensch MR, Wiemels JL, Nelson HH, Karagas MR, Padbury JF, Bueno R, Sugarbaker DJ, Yeh RF, Wiencke JK, Kelsey KT: Aging and environmental exposures alter tissue-specific DNA methylation dependent upon CpG island context. PLoS Genet 2009, 5:e1000602.View ArticlePubMedPubMed CentralGoogle Scholar
- Bollati V, Schwartz J, Wright R, Litonjua A, Tarantini L, Suh H, Sparrow D, Vokonas P, Baccarelli A: Decline in genomic DNA methylation through aging in a cohort of elderly subjects. Mech Ageing Dev 2009, 130:234–239.View ArticlePubMedGoogle Scholar
- Teschendorff AE, Menon U, Gentry-Maharaj A, Ramus SJ, Weisenberger DJ, Shen H, Campan M, Noushmehr H, Bell CG, Maxwell AP, Savage DA, Mueller-Holzner E, Marth C, Kocjan G, Gayther SA, Jones A, Beck S, Wagner W, Laird PW, Jacobs IJ, Widschwendter M: Age-dependent DNA methylation of genes that are suppressed in stem cells is a hallmark of cancer. Genome Res 2010, 20:440–446.View ArticlePubMedPubMed CentralGoogle Scholar
- Mugatroyd C, Wu Y, Bockmühl Y, Spengler D: The Janus face of DNA methylation in aging. Aging 2010, 2:107–110.View ArticlePubMed CentralGoogle Scholar
- Rodríguez-Rodero S, Fernández-Morera J, Fernandez A, Menéndez-Torre E, Fraga M: Epigenetic regulation of aging. Discov Med 2010, 10:225–233.PubMedGoogle Scholar
- Bell JT, Tsai PC, Yang TP, Pidsley R, Nisbet J, Glass D, Mangino M, Zhai G, Zhang F, Valdes A, Shin SY, Dempster EL, Murray RM, Grundberg E, Hedman AK, Nica A, Small KS, Dermitzakis ET, McCarthy MI, Mill J, Spector TD, Deloukas P, MuTHER Consortium: Epigenome-wide scans identify differentially methylated regions for age and age-related phenotypes in a healthy ageing population. PLoS Genet 2012, 8:e1002629.View ArticlePubMedPubMed CentralGoogle Scholar
- Horvath S, Zhang Y, Langfelder P, Kahn R, Boks M, van Eijk K, van den Berg L, Ophoff RA: Aging effects on DNA methylation modules in human brain and blood tissue. Genome Biol 2012, 13:R97.View ArticlePubMedPubMed CentralGoogle Scholar
- Rakyan VK, Down TA, Maslau S, Andrew T, Yang TP, Beyan H, Whittaker P, McCann OT, Finer S, Valdes AM, Leslie RD, Deloukas P, Spector TD: Human aging-associated DNA hypermethylation occurs preferentially at bivalent chromatin domains. Genome Res 2010, 20:434–439.View ArticlePubMedPubMed CentralGoogle Scholar
- Bernstein BE, Stamatoyannopoulos JA, Costello JF, Ren B, Milosavljevic A, Meissner A, Kellis M, Marra MA, Beaudet AL, Ecker JR, Farnham PJ, Hirst M, Lander ES, Mikkelsen TS, Thomson JA: The NIH roadmap epigenomics mapping consortium. Nat Biotechnol 2010, 28:1045–1048.View ArticlePubMedPubMed CentralGoogle Scholar
- Illingworth R, Kerr A, Desousa D, Jørgensen H, Ellis P, Stalker J, Jackson D, Clee C, Plumb R, Rogers J, Humphray S, Cox T, Langford C, Bird A: A novel CpG island set identifies tissue-specific methylation at developmental gene loci. PLoS Biol 2008, 6:e22.View ArticlePubMedPubMed CentralGoogle Scholar
- Li Y, Zhu J, Tian G, Li N, Li Q, Ye M, Zheng H, Yu J, Wu H, Sun J, Zhang H, Chen Q, Luo R, Chen M, He Y, Jin X, Zhang Q, Yu C, Zhou G, Sun J, Huang Y, Zheng H, Cao H, Zhou X, Guo S, Hu X, Li X, Kristiansen K, Bolund L, Xu J, et al.: The DNA methylome of human peripheral blood mononuclear cells. PLoS Biol 2010, 8:e1000533.View ArticlePubMedPubMed CentralGoogle Scholar
- Thompson RF, Atzmon G, Gheorghe C, Liang HQ, Lowes C, Greally JM, Barzilai N: Tissue-specific dysregulation of DNA methylation in aging. Aging Cell 2010, 9:506–518.View ArticlePubMedPubMed CentralGoogle Scholar
- Hernandez DG, Nalls MA, Gibbs JR, Arepalli S, van der Brug M, Chong S, Moore M, Longo DL, Cookson MR, Traynor BJ, Singleton AB: Distinct DNA methylation changes highly correlated with chronological age in the human brain. Hum Mol Genet 2011, 20:1164–1172.View ArticlePubMedPubMed CentralGoogle Scholar
- Koch C, Wagner W: Epigenetic-aging-signature to determine age in different tissues. Aging 2011, 3:1018–1027.View ArticlePubMedPubMed CentralGoogle Scholar
- Numata S, Ye T, Hyde Thomas M, Guitart-Navarro X, Tao R, Wininger M, Colantuoni C, Weinberger Daniel R, Kleinman Joel E, Lipska Barbara K: DNA methylation signatures in development and aging of the human prefrontal cortex. Am J Hum Genet 2012, 90:260–272.View ArticlePubMedPubMed CentralGoogle Scholar
- Bocklandt S, Lin W, Sehl ME, Sanchez FJ, Sinsheimer JS, Horvath S, Vilain E: Epigenetic predictor of age. PLoS One 2011, 6:e14821.View ArticlePubMedPubMed CentralGoogle Scholar
- Hannum G, Guinney J, Zhao L, Zhang L, Hughes G, Sadda S, Klotzle B, Bibikova M, Fan JB, Gao Y, Deconde R, Chen M, Rajapakse I, Friend S, Ideker T, Zhang K: Genome-wide methylation profiles reveal quantitative views of human aging rates. Mol Cell 2013, 49:359–367.View ArticlePubMedGoogle Scholar
- Laird PW: The power and the promise of DNA methylation markers. Nat Rev Cancer 2003, 3:253–266.View ArticlePubMedGoogle Scholar
- Bjornsson HT, Sigurdsson MI, Fallin MD, Irizarry RA, Aspelund T, Cui H, Yu W, Rongione MA, Ekström TJ, Harris TB, Launer LJ, Eiriksdottir G, Leppert MF, Sapienza C, Gudnason V, Feinberg AP: Intra-individual change over time in DNA methylation with familial clustering. JAMA 2008, 299:2877–2883.View ArticlePubMedPubMed CentralGoogle Scholar
- Pai AA, Bell JT, Marioni JC, Pritchard JK, Gilad Y: A genome-wide study of DNA methylation patterns and gene expression levels in multiple human and Chimpanzee tissues. PLoS Genet 2011, 7:e1001316.View ArticlePubMedPubMed CentralGoogle Scholar
- Hernando-Herraez I, Prado-Martinez J, Garg P, Fernandez-Callejo M, Heyn H, Hvilsom C, Navarro A, Esteller M, Sharp A, Marques-Bonet T: Dynamics of DNA methylation in recent human and great apes evolution. PLoS Genet 2013, 9:e1003763.View ArticlePubMedPubMed CentralGoogle Scholar
- Ernst J, Kheradpour P, Mikkelsen TS, Shoresh N, Ward LD, Epstein CB, Zhang X, Wang L, Issner R, Coyne M, Ku M, Durham T, Kellis M, Bernstein BE: Mapping and analysis of chromatin state dynamics in nine human cell types. Nature 2011, 473:43–49.View ArticlePubMedPubMed CentralGoogle Scholar
- Adkins RM, Krushkal J, Tylavsky FA, Thomas F: Racial differences in gene-specific DNA methylation levels are present at birth. Birth Defects Res A Clin Mol Teratol 2011, 91:728–736.View ArticlePubMedPubMed CentralGoogle Scholar
- Bell J, Pai A, Pickrell J, Gaffney D, Pique-Regi R, Degner J, Gilad Y, Pritchard J: DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines. Genome Biol 2011, 12:R10.View ArticlePubMedPubMed CentralGoogle Scholar
- Fraser H, Lam L, Neumann S, Kobor M: Population-specificity of human DNA methylation. Genome Biol 2012, 13:R8.View ArticlePubMedPubMed CentralGoogle Scholar
- van Eijk KR, de Jong S, Boks MP, Langeveld T, Colas F, Veldink JH, de Kovel CG, Janson E, Strengman E, Langfelder P, Kahn RS, van den Berg LH, Horvath S, Ophoff RA: Genetic analysis of DNA methylation and gene expression levels in whole blood of healthy human subjects. BMC Genomics 2012, 13:636.View ArticlePubMedPubMed CentralGoogle Scholar
- Jones M, Fejes A, Kobor M: DNA methylation, genotype and gene expression: who is driving and who is along for the ride? Genome Biol 2013, 14:126.View ArticlePubMedPubMed CentralGoogle Scholar
- Shibata D, Tavaré S: Counting divisions in a human somatic cell tree: how, what and why. Cell Cycle 2006, 5:610–614.View ArticlePubMedGoogle Scholar
- Richardson B: Impact of aging on DNA methylation. Ageing Res Rev 2003, 2:245–261.View ArticlePubMedGoogle Scholar
- Kim JY, Tavaré S, Shibata D: Counting human somatic cell replications: methylation mirrors endometrial stem cell divisions. Proc Natl Acad Sci U S A 2005, 102:17739–17744.View ArticlePubMedPubMed CentralGoogle Scholar
- Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts. Science 1998, 282:1145–1147.View ArticlePubMedGoogle Scholar
- Hinoue T, Weisenberger DJ, Lange CP, Shen H, Byun HM, Van Den Berg D, Malik S, Pan F, Noushmehr H, van Dijk CM, Tollenaar RA, Laird PW: Genome-scale analysis of aberrant DNA methylation in colorectal cancer. Genome Res 2012, 22:271–282.View ArticlePubMedPubMed CentralGoogle Scholar
- Schwartzentruber J, Korshunov A, Liu XY, Jones DT, Pfaff E, Jacob K, Sturm D, Fontebasso AM, Quang DA, Tönjes M, Hovestadt V, Albrecht S, Kool M, Nantel A, Konermann C, Lindroth A, Jäger N, Rausch T, Ryzhova M, Korbel JO, Hielscher T, Hauser P, Garami M, Klekner A, Bognar L, Ebinger M, Schuhmann MU, Scheurlen W, Pekrun A, Frühwald MC, et al.: Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma. Nature 2012, 482:226–231.View ArticlePubMedGoogle Scholar
- Bernstein BE, Mikkelsen TS, Xie X, Kamal M, Huebert DJ, Cuff J, Fry B, Meissner A, Wernig M, Plath K, Jaenisch R, Wagschal A, Feil R, Schreiber SL, Lander ES: A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell 2006, 125:315–326.View ArticlePubMedGoogle Scholar
- Kolasinska-Zwierz P, Down T, Latorre I, Liu T, Liu XS, Ahringer J: Differential chromatin marking of introns and expressed exons by H3K36me3. Nat Genet 2009, 41:376–381.View ArticlePubMedPubMed CentralGoogle Scholar
- Bjerke L, Mackay A, Nandhabalan M, Burford A, Jury A, Popov S, Bax DA, Carvalho D, Taylor KR, Vinci M, Bajrami I, McGonnell IM, Lord CJ, Reis RM, Hargrave D, Ashworth A, Workman P, Jones C: Histone H3.3 Mutations drive pediatric glioblastoma through upregulation of MYCN. Cancer Discov 2013, 3:512–519.View ArticlePubMedPubMed CentralGoogle Scholar
- Sturm D, Witt H, Hovestadt V, Khuong-Quang DA, Jones DT, Konermann C, Pfaff E, Tönjes M, Sill M, Bender S, Kool M, Zapatka M, Becker N, Zucknick M, Hielscher T, Liu XY, Fontebasso AM, Ryzhova M, Albrecht S, Jacob K, Wolter M, Ebinger M, Schuhmann MU, van Meter T, Frühwald MC, Hauch H, Pekrun A, Radlwimmer B, Niehues T, von Komorowski G, Dürken M, et al.: Hotspot mutations in H3F3A and IDH1 define distinct epigenetic and biological subgroups of glioblastoma. Cancer Cell 2012, 22:425–437.View ArticlePubMedGoogle Scholar
- Friedman J, Hastie T, Tibshirani R: Regularization paths for generalized linear models via coordinate descent. J Stat Softw 2010, 33:1–22.View ArticlePubMedPubMed CentralGoogle Scholar
- Alisch RS, Barwick BG, Chopra P, Myrick LK, Satten GA, Conneely KN, Warren ST: Age-associated DNA methylation in pediatric populations. Genome Res 2012, 22:623–632.View ArticlePubMedPubMed CentralGoogle Scholar
- Harris RA, Nagy-Szakal D, Pedersen N, Opekun A, Bronsky J, Munkholm P, Jespersgaard C, Andersen P, Melegh B, Ferry G, Jess T, Kellermayer R: Genome-wide peripheral blood leukocyte DNA methylation microarrays identified a single association with inflammatory bowel diseases. Inflamm Bowel Dis 2012, 18:2334–2341.View ArticlePubMedGoogle Scholar
- Gibbs JR, van der Brug MP, Hernandez DG, Traynor BJ, Nalls MA, Lai SL, Arepalli S, Dillman A, Rafferty IP, Troncoso J, Johnson R, Zielke HR, Ferrucci L, Longo DL, Cookson MR, Singleton AB: Abundant quantitative trait loci exist for DNA methylation and gene expression in human brain. PLoS Genet 2010, 6:e1000952.View ArticlePubMedPubMed CentralGoogle Scholar
- Guintivano J, Aryee MJ, Kaminsky ZA: A cell epigenotype specific model for the correction of brain cellular heterogeneity bias and its application to age, brain region and major depression. Epigenetics 2013, 8:290–302.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhuang J, Jones A, Lee SH, Ng E, Fiegl H, Zikan M, Cibula D, Sargent A, Salvesen HB, Jacobs IJ, Kitchener HC, Teschendorff AE, Widschwendter M: The dynamics and prognostic potential of DNA methylation changes at stem cell gene loci in women’s cancer. PLoS Genet 2012, 8:e1002517.View ArticlePubMedPubMed CentralGoogle Scholar
- Essex MJ, Thomas Boyce W, Hertzman C, Lam LL, Armstrong JM, Neumann SMA, Kobor MS: Epigenetic vestiges of early developmental adversity: childhood stress exposure and DNA methylation in adolescence. Child Dev 2011, 84:58–75.View ArticlePubMedPubMed CentralGoogle Scholar
- Martino DJ, Tulic MK, Gordon L, Hodder M, Richman T, Metcalfe J, Prescott SL, Saffery R: Evidence for age-related and individual-specific changes in DNA methylation profile of mononuclear cells during early immune development in humans. Epigenetics 2011, 6:1085–1094.View ArticlePubMedGoogle Scholar
- Fernández-Tajes J, Soto-Hermida A, Vázquez-Mosquera ME, Cortés-Pereira E, Mosquera A, Fernández-Moreno M, Oreiro N, Fernández-López C, Fernández JL: Rego-Pérez I. Blanco FJ: Genome-wide DNA methylation analysis of articular chondrocytes reveals a cluster of osteoarthritic patients. Ann Rheum Dis; 2013. Epub ahead of printGoogle Scholar
- Harris RA, Nagy-Szakal D, Kellermayer R: Human metastable epiallele candidates link to common disorders. Epigenetics 2013, 8:157–163.View ArticlePubMedPubMed CentralGoogle Scholar
- Grönniger E, Weber B, Heil O, Peters N, Stäb F, Wenck H, Korn B, Winnefeld M, Lyko F: Aging and chronic sun exposure cause distinct epigenetic changes in human skin. PLoS Genet 2010, 6:e1000971.View ArticlePubMedPubMed CentralGoogle Scholar
- Zouridis H, Deng N, Ivanova T, Zhu Y, Wong B, Huang D, Wu YH, Wu Y, Tan IB, Liem N, Gopalakrishnan V, Luo Q, Wu J, Lee M, Yong WP, Goh LK, Teh BT, Rozen S, Tan P: Methylation subtypes and large-scale epigenetic alterations in gastric cancer. Sci Transl Med 2012, 4:156ra140.View ArticlePubMedGoogle Scholar
- Haas J, Frese KS, Park YJ, Keller A, Vogel B, Lindroth AM, Weichenhan D, Franke J, Fischer S, Bauer A, Marquart S, Sedaghat-Hamedani F, Kayvanpour E, Köhler D, Wolf NM, Hassel S, Nietsch R, Wieland T, Ehlermann P, Schultz JH, Dösch A, Mereles D, Hardt S, Backs J, Hoheisel JD, Plass C, Katus HA, Meder B: Alterations in cardiac DNA methylation in human dilated cardiomyopathy. EMBO Mol Med 2013, 5:413–429.View ArticlePubMedPubMed CentralGoogle Scholar
- Shen J, Wang S, Zhang YJ, Kappil M, Wu HC, Kibriya MG, Wang Q, Jasmine F, Ahsan H, Lee PH, Yu MW, Chen CJ, Santella RM: Genome-wide DNA methylation profiles in hepatocellular carcinoma. Hepatology 2012, 55:1799–1808.View ArticlePubMedPubMed CentralGoogle Scholar
- Bork S, Pfister S, Witt H, Horn P, Korn B, Ho A, Wagner W: DNA methylation pattern changes upon long-term culture and aging of human mesenchymal stromal cells. Aging Cell 2010, 9:54–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Gordon L, Joo JE, Powell JE, Ollikainen M, Novakovic B, Li X, Andronikos R, Cruickshank MN, Conneely KN, Smith AK, Alisch RS, Morley R, Visscher PM, Craig JM, Saffery R: Neonatal DNA methylation profile in human twins is specified by a complex interplay between intrauterine environmental and genetic factors, subject to tissue-specific influence. Genome Res 2012, 22:1395–1406.View ArticlePubMedPubMed CentralGoogle Scholar
- Kobayashi Y, Absher DM, Gulzar ZG, Young SR, McKenney JK, Peehl DM, Brooks JD, Myers RM, Sherlock G: DNA methylation profiling reveals novel biomarkers and important roles for DNA methyltransferases in prostate cancer. Genome Res 2011, 21:1017–1027.View ArticlePubMedPubMed CentralGoogle Scholar
- Liu J, Morgan M, Hutchison K, Calhoun VD: A study of the influence of sex on genome wide methylation. PLoS One 2010, 5:e10028.View ArticlePubMedPubMed CentralGoogle Scholar
- Song H, Ramus SJ, Tyrer J, Bolton KL, Gentry-Maharaj A, Wozniak E, Anton-Culver H, Chang-Claude J, Cramer DW, DiCioccio R, Dörk T, Goode EL, Goodman MT, Schildkraut JM, Sellers T, Baglietto L, Beckmann MW, Beesley J, Blaakaer J, Carney ME, Chanock S, Chen Z, Cunningham JM, Dicks E, Doherty JA, Dürst M, Ekici AB, Fenstermacher D, Fridley BL, Giles G, et al.: A genome-wide association study identifies a new ovarian cancer susceptibility locus on 9p22.2. Nat Genet 2009, 41:996–1000.View ArticlePubMedPubMed CentralGoogle Scholar
- Liu Y, Aryee MJ, Padyukov L, Fallin MD, Hesselberg E, Runarsson A, Reinius L, Acevedo N, Taub M, Ronninger M, Shchetynsky K, Scheynius A, Kere J, Alfredsson L, Klareskog L, Ekström TJ, Feinberg AP: Epigenome-wide association data implicate DNA methylation as an intermediary of genetic risk in rheumatoid arthritis. Nat Biotech 2013, 31:142–147.View ArticleGoogle Scholar
- Heyn H, Li N, Ferreira HJ, Moran S, Pisano DG, Gomez A, Diez J, Sanchez-Mut JV, Setien F, Carmona FJ, Puca AA, Sayols S, Pujana MA, Serra-Musach J, Iglesias-Platas I, Formiga F, Fernandez AF, Fraga MF, Heath SC, Valencia A, Gut IG, Wang J, Esteller M: Distinct DNA methylomes of newborns and centenarians. Proc Natl Acad Sci U S A 2012, 109:10522–10527.View ArticlePubMedPubMed CentralGoogle Scholar
- Lam LL, Emberly E, Fraser HB, Neumann SM, Chen E, Miller GE, Kobor MS: Factors underlying variable DNA methylation in a human community cohort. Proc Natl Acad Sci U S A 2012, 109:17253–17260.View ArticlePubMedPubMed CentralGoogle Scholar
- Khulan B, Cooper WN, Skinner BM, Bauer J, Owens S, Prentice AM, Belteki G, Constancia M, Dunger D, Affara NA: Periconceptional maternal micronutrient supplementation is associated with widespread gender related changes in the epigenome: a study of a unique resource in the Gambia. Hum Mol Genet 2012, 21:2086–2101.View ArticlePubMedGoogle Scholar
- Martino D, Maksimovic J, Joo JH, Prescott SL, Saffery R: Genome-scale profiling reveals a subset of genes regulated by DNA methylation that program somatic T-cell phenotypes in humans. Genes Immun 2012, 13:388–398.View ArticlePubMedGoogle Scholar
- Heyn H, Moran S, Esteller M: Aberrant DNA methylation profiles in the premature aging disorders Hutchinson-Gilford Progeria and Werner syndrome. Epigenetics 2013, 8:28–33.View ArticlePubMedPubMed CentralGoogle Scholar
- Ginsberg MR, Rubin RA, Falcone T, Ting AH, Natowicz MR: Brain transcriptional and epigenetic associations with autism. PLoS One 2012, 7:e44736.View ArticlePubMedPubMed CentralGoogle Scholar
- Martino D, Loke Y, Gordon L, Ollikainen M, Cruickshank M, Saffery R, Craig J: Longitudinal, genome-scale analysis of DNA methylation in twins from birth to 18 months of age reveals rapid epigenetic change in early life and pair-specific effects of discordance. Genome Biol 2013, 14:R42.View ArticlePubMedPubMed CentralGoogle Scholar
- Ribel-Madsen R, Fraga MF, Jacobsen S, Bork-Jensen J, Lara E, Calvanese V, Fernandez AF, Friedrichsen M, Vind BF, Højlund K, Beck-Nielsen H, Esteller M, Vaag A, Poulsen P: Genome-wide analysis of DNA methylation differences in muscle and Fat from monozygotic twins discordant for Type 2 diabetes. PLoS One 2012, 7:e51302.View ArticlePubMedPubMed CentralGoogle Scholar
- Jacobsen SC, Brøns C, Bork-Jensen J, Ribel-Madsen R, Yang B, Lara E, Hall E, Calvanese V, Nilsson E, Jørgensen SW, Mandrup S, Ling C, Fernandez AF, Fraga MF, Poulsen P, Vaag A: Effects of short-term high-fat overfeeding on genome-wide DNA methylation in the skeletal muscle of healthy young men. Diabetologia 2012, 55:3341–3349.View ArticlePubMedGoogle Scholar
- Blair JD, Yuen RKC, Lim BK, McFadden DE, von Dadelszen P, Robinson WP: Widespread DNA hypomethylation at gene enhancer regions in placentas associated with early-onset pre-eclampsia. Mol Hum Reprod 2013, 19:697–708.View ArticlePubMedPubMed CentralGoogle Scholar
- Teschendorff A, Jones A, Fiegl H, Sargent A, Zhuang J, Kitchener H, Widschwendter M: Epigenetic variability in cells of normal cytology is associated with the risk of future morphological transformation. Genome Med 2012, 4:24.View ArticlePubMedPubMed CentralGoogle Scholar
- Pacheco SE, Houseman EA, Christensen BC, Marsit CJ, Kelsey KT, Sigman M, Boekelheide K: Integrative DNA methylation and gene expression analyses identify DNA packaging and epigenetic regulatory genes associated with low motility sperm. PLoS One 2011, 6:e20280.View ArticlePubMedPubMed CentralGoogle Scholar
- Krausz C, Sandoval J, Sayols S, Chianese C, Giachini C, Heyn H, Esteller M: Novel insights into DNA methylation features in spermatozoa: stability and peculiarities. PLoS One 2012, 7:e44479.View ArticlePubMedPubMed CentralGoogle Scholar
- Nazor KL, Altun G, Lynch C, Tran H, Harness JV, Slavin I, Garitaonandia I, Müller FJ, Wang YC, Boscolo FS, Fakunle E, Dumevska B, Lee S, Park HS, Olee T, D’Lima DD, Semechkin R, Parast MM, Galat V, Laslett AL, Schmidt U, Keirstead HS, Loring JF, Laurent LC: Recurrent variations in DNA methylation in human pluripotent stem cells and their differentiated derivatives. Cell stem cell 2012, 10:620–634.View ArticlePubMedPubMed CentralGoogle Scholar
- Shao K, Koch C, Gupta MK, Lin Q, Lenz M, Laufs S, Denecke B, Schmidt M, Linke M, Hennies HC, Hescheler J, Zenke M, Zechner U, Šarić T, Wagner W: Induced pluripotent mesenchymal stromal cell clones retain donor-derived differences in DNA methylation profiles. Mol Ther 2013, 21:240–250.View ArticlePubMedGoogle Scholar
- Calvanese V, Fernández AF, Urdinguio RG, Suárez-Alvarez B, Mangas C, Pérez-García V, Bueno C, Montes R, Ramos-Mejía V, Martínez-Camblor P, Ferrero C, Assenov Y, Bock C, Menendez P, Carrera AC, Lopez-Larrea C, Fraga MF: A promoter DNA demethylation landscape of human hematopoietic differentiation. Nucleic Acids Res 2012, 40:116–131.View ArticlePubMedGoogle Scholar
- Ramos-Mejía V, Fernandez A, Ayllon V, Real P, Bueno C, Anderson P, Martín F, Fraga M, Menendez P: Maintenance of human embryonic stem cells in mesenchymal stem cell-conditioned media augments hematopoietic specification. Stem Cells Dev 2012, 21:1549–1558.View ArticlePubMedGoogle Scholar
- Reinius LE, Acevedo N, Joerink M, Pershagen G, Dahlén S-E, Greco D, Söderhäll C, Scheynius A, Kere J: Differential DNA methylation in purified human blood cells: implications for cell lineage and studies on disease susceptibility. PLoS One 2012, 7:e41361.View ArticlePubMedPubMed CentralGoogle Scholar
- Fackler MJ, Umbricht CB, Williams D, Argani P, Cruz LA, Merino VF, Teo WW, Zhang Z, Huang P, Visvananthan K, Marks J, Ethier S, Gray JW, Wolff AC, Cope LM, Sukumar S: Genome-wide methylation analysis identifies genes specific to breast cancer hormone receptor status and risk of recurrence. Cancer Res 2011, 71:6195–6207.View ArticlePubMedPubMed CentralGoogle Scholar
- Dedeurwaerder S, Desmedt C, Calonne E, Singhal SK, Haibe-Kains B, Defrance M, Michiels S, Volkmar M, Deplus R, Luciani J, Lallemand F, Larsimont D, Toussaint J, Haussy S, Rothé F, Rouas G, Metzger O, Majjaj S, Saini K, Putmans P, Hames G, van Baren N, Coulie PG, Piccart M, Sotiriou C, Fuks F: DNA methylation profiling reveals a predominant immune component in breast cancers. EMBO Mol Med 2011, 3:726–741.View ArticlePubMedPubMed CentralGoogle Scholar
- Lauss M, Aine M, Sjödahl G, Veerla S, Patschan O, Gudjonsson S, Chebil G, Lövgren K, Fernö M, Månsson W, Liedberg F, Ringnér M, Lindgren D, Höglund M: DNA methylation analyses of urothelial carcinoma reveal distinct epigenetic subtypes and an association between gene copy number and methylation status. Epigenetics 2012, 7:858–867.View ArticlePubMedPubMed CentralGoogle Scholar
- Langfelder P, Mischel PS, Horvath S: When is hub gene selection better than standard meta-analysis? PLoS One 2013, 8:e61505.View ArticlePubMedPubMed CentralGoogle Scholar
- Lee TI, Jenner RG, Boyer LA, Guenther MG, Levine SS, Kumar RM, Chevalier B, Johnstone SE, Cole MF, Isono K, Koseki H, Fuchikami T, Abe K, Murray HL, Zucker JP, Yuan B, Bell GW, Herbolsheimer E, Hannett NM, Sun K, Odom DT, Otte AP, Volkert TL, Bartel DP, Melton DA, Gifford DK, Jaenisch R, Young RA: Control of developmental regulators by polycomb in human embryonic stem cells. Cell 2006, 125:301–313.View ArticlePubMedPubMed CentralGoogle Scholar
- Miller JA, Cai C, Langfelder P, Geschwind DH, Kurian SM, Salomon DR, Horvath S: Strategies for aggregating gene expression data: the collapseRows R function. BMC Bioinforma 2011, 12:322.View ArticleGoogle Scholar
- Teschendorff AE, Marabita F, Lechner M, Bartlett T, Tegner J, Gomez-Cabrero D, Beck S: A beta-mixture quantile normalization method for correcting probe design bias in Illumina Infinium 450 k DNA methylation data. Bioinformatics 2013, 29:189–196.View ArticlePubMedGoogle Scholar
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