Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum
- Richard Sucgang†1,
- Alan Kuo†2,
- Xiangjun Tian†3,
- William Salerno†1,
- Anup Parikh4,
- Christa L Feasley5,
- Eileen Dalin2,
- Hank Tu2,
- Eryong Huang4,
- Kerrie Barry2,
- Erika Lindquist2,
- Harris Shapiro2,
- David Bruce2,
- Jeremy Schmutz2,
- Asaf Salamov2,
- Petra Fey6,
- Pascale Gaudet6,
- Christophe Anjard7,
- M Madan Babu8,
- Siddhartha Basu6,
- Yulia Bushmanova6,
- Hanke van der Wel5,
- Mariko Katoh-Kurasawa4,
- Christopher Dinh1,
- Pedro M Coutinho9,
- Tamao Saito10,
- Marek Elias11,
- Pauline Schaap12,
- Robert R Kay8,
- Bernard Henrissat9,
- Ludwig Eichinger13,
- Francisco Rivero14,
- Nicholas H Putnam3,
- Christopher M West5,
- William F Loomis7,
- Rex L Chisholm6,
- Gad Shaulsky3, 4,
- Joan E Strassmann3,
- David C Queller3,
- Adam Kuspa1, 3, 4Email author and
- Igor V Grigoriev2
© Sucgang et al.; licensee BioMed Central Ltd. 2011
Received: 20 July 2010
Accepted: 28 February 2011
Published: 28 February 2011
The social amoebae (Dictyostelia) are a diverse group of Amoebozoa that achieve multicellularity by aggregation and undergo morphogenesis into fruiting bodies with terminally differentiated spores and stalk cells. There are four groups of dictyostelids, with the most derived being a group that contains the model species Dictyostelium discoideum.
We have produced a draft genome sequence of another group dictyostelid, Dictyostelium purpureum, and compare it to the D. discoideum genome. The assembly (8.41 × coverage) comprises 799 scaffolds totaling 33.0 Mb, comparable to the D. discoideum genome size. Sequence comparisons suggest that these two dictyostelids shared a common ancestor approximately 400 million years ago. In spite of this divergence, most orthologs reside in small clusters of conserved synteny. Comparative analyses revealed a core set of orthologous genes that illuminate dictyostelid physiology, as well as differences in gene family content. Interesting patterns of gene conservation and divergence are also evident, suggesting function differences; some protein families, such as the histidine kinases, have undergone little functional change, whereas others, such as the polyketide synthases, have undergone extensive diversification. The abundant amino acid homopolymers encoded in both genomes are generally not found in homologous positions within proteins, so they are unlikely to derive from ancestral DNA triplet repeats. Genes involved in the social stage evolved more rapidly than others, consistent with either relaxed selection or accelerated evolution due to social conflict.
The findings from this new genome sequence and comparative analysis shed light on the biology and evolution of the Dictyostelia.
The social amoebae have been used to study mechanisms of eukaryotic cell chemotaxis and cell differentiation for over 70 years. The completion of the Dictyostelium discoideum genome sequence provided a wealth of information about the basic cell and developmental biology of these organisms and highlighted an unexpected similarity between the cell motility and signaling systems of the social amoebae and the metazoa . For example, the D. discoideum genome encodes numerous G-protein coupled receptors (GPCRs) of the frizzled/smoothened, metabotropic glutamate, and secretin families that were previously thought to be specific to animals, suggesting that the GPCR gene families branched prior to the animal/fungal split. Numerous other examples, such as SH2 domain based phosphoprotein signaling, the full complement of ATP-binding cassette (ABC) transporter gene families, and the apparently complex actin cytoskeleton, served to strengthen the idea that amoeba and amoeboid animal cells are related in a more fundamental way than one might have guessed based on their gross physiological traits. We compared the D. discoideum genome with a second dictyostelid genome, that of Dictyostelium purpureum, in order to determine the set of genes they share, as well as their genomic differences that might illuminate variations in physiology within the social amoeba.
The Amoebozoa are closely related to the opisthokonts (animals and fungi) and include unicellular amoebae (for example, Acanthamoeba castellani), obligate parasitic amoeba (for example, Entamoeba histolytica), the true slime molds (for example, Physarum polycephalum) and the social amoebae, or Dictyostelia (often incorrectly referred to as 'slime molds'). In the 10 years since the monophyly of the Amoebozoa was proposed , genomic-scale analysis has confirmed the hypothesis  and the phylogenetic relationships between the major amoeboid lineages have been clarified [4–6]. A molecular phylogeny of the Dictyostelia has been constructed and suggests four major groups; the basal, group 1 parvisporids that produce small spores; the group 2 heterostelids; the group 3 rhizostelids; and the group 4 dictyostelids, which include D. purpureum and the well-studied D. discoideum . The dictyostelid group contains the largest number of described species of social amoeba and all of them produce large fruiting bodies with single sori, containing oblong spores, held aloft on a single cellular stalk.
D. purpureum differs from D. discoideum in a number of developmental and morphological ways . In particular, during the social stage, D. discoideum delays irreversible commitment by cells to sterile stalk tissue until slug migration is complete. D. purpureum, by contrast, forms a stalk of dead cells as the slug moves towards light, increasing its ability to cross gaps . In addition, D. purpureum makes taller fruiting bodies with smaller spores than D. discoideum . D. purpureum fruiting bodies are purple with a triangular base formed from specialized stalk cells, whereas D. discoideum fruiting bodies are yellow and supported by a basal disc. D. purpureum also exhibits greater sorting into kin groups in the social stage than does D. discoideum [10, 11].
The D. discoideum genome sequence was the first amoebozoan genome to become available, and the deduced gene list improved our understanding of the facultative multicellular lifestyle of the social amoeba [1, 12]. Here we present our initial analysis of the D. purpureum genome and compare it to the D. discoideum genome. Since these two species represent the two major clades of the group 4 dictyostelids, a comparison of their genomes has revealed much of the genomic diversity and conservation within this group of social amoebae. Overall, the two genomes are similar in size and gene content, sharing at least 7,619 orthologous protein coding genes and many more paralogous genes. A global analysis of sequence divergence suggests that the genetic diversity of the dictyostelids is similar to that of the vertebrates, from the bony fishes to the mammals. Some large gene families are nearly completely conserved between these two dictyostelids, while others have markedly diverged. Our analyses highlight general characteristics that are conserved among the dictyostelids, as well as potential differences, linking the genomic potential with the physiology of these soil microbes.
Results and Discussion
Structure and comparative genomics of the D. purpureumgenome
Comparison between the predicted protein coding genes of D. purpureu m and D. discoideu m
D. discoideum a
Genome size (Mb)
Number of genes
Gene density (kb per gene)
Mean gene length (nucleotides)
Intron per gene (spliced genes)
Mean intron length (nucleotides)
Mean protein length (amino acids)
Repetitive elements and simple sequence repeats
The D. purpureum genome contains 1.1 Mb of transposons (3.4%), fewer than in D. discoideum. The largest families of transposons are Gypsy (approximately 400 kb, 35.8% of total transposons), Mariner (approximately 186 kb, 16.7%), MSAT1_Dpu (126 kb, 11.4%), and hAT (105 kb, 9.5%).
Amino acid homopolymers
Codon usage within these amino acid homopolymers is quite similar to codon usage for the same amino acids outside of repeats, with a pattern quite similar to D. discoideum (Figure S2 in Additional file 1). Again, as in D. discoideum, many amino acid homopolymers contain a single codon, consistent with the relatively recent expansion of those triplet repeats. However, the codon diversity of D. purpureum amino acid repeats is significantly higher than it is for D. discoideum (Figure S3 in Additional file 1), consistent with the D. discoideum repeats being younger, with less time to accumulate changes from the original codon.
The potential function of most amino acid repeats is unknown, but the availability of the D. purpureum genome permits some new tests. If amino acid repeats are generally functionally important, they should tend to be conserved in their position within orthologous proteins. Sixty-four percent of the 2,645 D. purpureum amino acid repeats and 68% of the 11,243 D. discoideum repeats occur in genes that do not have homologs in the other species. Even in those with orthologs, only 19% of D. purpureum repeats and 5% of the D. discoideum repeats appeared to be homologous within global alignments of their respective proteins. The count of homologous repeats would be higher if we included matches where at least one falls below the threshold expectation for non-random homopolymers (for example, a match between 25 asparagines in D. discoideum and 8 in D. purpureum would be excluded as a chance event; P > 0.01; Table S1 in Additional file 1). On the other hand, some could be fortuitous matches forced by a large number of repeated amino acids that are not truly homologous. Inspection of selected sequences shows at least some that appear to be convincing homologs, with strong identity on both sides of the repeat (Figure S4 in Additional file 1). Still, the apparent small fraction of homologous repeats suggests that the very similar patterns of amino acid homopolymer abundance and distribution do not come primarily from conserved ancestral repeats. Instead they may come from some shared physiological properties - perhaps distinctive DNA polymerases or repair enzymes or high AT-content - that generate similar patterns independently.
In addition to the lack of homology for amino acid homopolymers between D. discoideum and D. purpureum, several pieces of evidence suggest that these triplet repeats may be 'junk' that accumulates due to weak selection on proteins that are relatively unimportant for fitness. For genes that have homologs in the two species, those with amino acid repeats in either species have higher non-synonymous substitution rates in the non-repeat regions, as expected if genes with repeats are generally less subject to purifying selection (Figure 2b). Another indicator of the degree of selective constraint on a gene is its expression level, particularly in the single-celled, vegetative stage where the selective pressure is likely to be the greatest. If amino acid repeats accumulate in genes where selective constraints are low, we would predict that they will be more common in genes expressed in the social or developmental stages, as opposed to vegetative stages. Using the recent comparison of the transcriptional profiles of D. discoideum and D. purpureum development by RNA-seq analysis , this prediction is confirmed (Figure S5a,c in Additional file 1). Similarly, we would predict, looking only at RNA-seq reads from the vegetative stage, that genes coding for amino acid repeats would be less abundant and this is also confirmed (Figure S5b,d in Additional file 1). In sum, although a small number of repeats appear to be conserved over long periods of time, most appear to have arisen relatively recently in genes where selection against amino acid changes is weak.
Phylogeny of D. purpureum
Horizontal gene transfer
Candidate horizontal gene transfers from Bacteria
Function in bacteriab
D. discoideumdictyBase IDc
Function in D. discoideumc
D. purpureumprotein IDd
D. purpureumdictyBase ID
Aromatic amino acid lyase
colA, Colossin A slug protein
Insecticidal crystal protein
thyA, thymidylate synthesis
Functional information now exists for 6 of the 16 proposed HGT genes and it is interesting to see how the dictyostelids have utilized these contributions from bacteria. ThyA has completely replaced an essential enzyme in central metabolism . Since it is also present in the amoebozoan slime mold Physarum polycephalum (GenBank accession number [GenBank:AAY87038] ), the change over to the rare bacterial enzyme must have taken place quite early in the radiation of the amoebozoa. The isopentenyl transferase, IptA, produces discadenine, which is a sporulation inducer and spore germination inhibitor . Another gene, pscA, encodes an active penicillin-sensitive peptidase but its function is not known , and Ppk1 is a bacterial type polyphosphate synthase . Colossin A (ColA) appears to be a structural protein of the slug that was fashioned out of hundreds of repeats of a bacterial Cna_B domain . CapA and CapB are two cAMP-binding proteins whose carboxy-terminal half is derived from a subunit of a bacterial tellurium resistance complex . Recently, CapB was identified in a proteomic screen for centrosomal proteins .
Conserved gene order between the D. purpureum and D. discoideumgenomes
Genomes evolve through base substitution and insertion/deletion, and also through rearrangements that alter the order and orientation of genes on chromosomes. Synteny, the nature and extent of conserved gene order between species, serves as an important gauge of the dynamics of genome evolution . To characterize the potential synteny between D. purpureum and D. discoideum, we identified blocks of approximately conserved gene order between their genomes, and compared the number and sizes of these potential conserved syntenic blocks to control genomes in which the gene orders were artificially scrambled. Although the D. purpureum genome is not fully assembled, the current level of contiguity allows for an analysis of conserved gene order on a small scale (approximately 50 kb). Blocks of potential synteny were constructed by single-linkage clustering of D. purpureum genes, where pairs of genes are considered linked if (i) they fall on the same scaffold of the assembly with at most w intervening genes that have D. discoideum orthologs, and (ii) their D. discoideum orthologs all fall on a single chromosome, with no more than w intervening genes that have D. purpureum orthologs. For stretches of perfectly conserved gene order (blocks constructed with w = 0), 4,734 (63%) of the 1:1 ortholog pairs used in the analysis lie in a genomic block of conserved gene order involving at least two genes in each genome. The mean size of such blocks is 2.8 genes in each genome, with the longest perfectly conserved stretch containing 10 genes.
To determine the maximum length scale over which significant conservation of gene order persists, we compared the increase in potential syntenic clusters as a function of an increasing number of intervening genes (w) for D. purpureum versus D. discoideum to the rate obtained for the permutation controls (Figure S6 in Additional file 1). We found that for up to about 15 intervening genes, potential conserved gene clusters grow significantly faster than what is expected for the same two genomes with randomized gene orders, which provides a conservative threshold for identifying blocks of conserved gene order. With this estimate, 76% of orthologous gene pairs participate in a block of approximately conserved gene order, compared to 5.8 ± 0.4% in controls, with a false positive rate, on a gene-by-gene basis, of approximately 7%. The 5,793 genes contained in these blocks, and their positions in the genome, are listed in Additional file 2. This indicates that the majority of orthologs in D. purpureum and D. discoideum are found in small neighborhoods of exactly conserved gene order between the two species, and that these neighborhoods are themselves clustered into larger regions of approximately conserved gene order.
Gene content comparisons of D. purpureum and D. discoideumgenomes
Non-coding RNA genes
The described catalog of non-coding RNAs (ncRNAs) in the Dictyostelia was long limited to tRNAs, rRNAs, and a handful of experimentally identified short RNAs, all found in D. discoideum (for review, see ). Recent work has expanded this repertoire to include a family of spliceosomal ncRNAs and two classes (class I and class II) of novel ncRNAs [30, 31]. The spliceosomal RNAs identified in D. discoideum, U1, U2, U4, U5, and U6, are each characterized by both specific RNA-binding motifs and the ability to fold into characterized secondary structures [30, 31]. Using a modified BLAST search (Additional file 1), we have identified a set of D. purpureum spliceosomal homologs that are predicted to fold into the appropriate secondary structures (Table S3a in Additional file 1).
In D. discoideum a 'Dictyostelium upstream sequence element' (DUSE) has been described that sits approximately 63 bp upstream of many ncRNAs, including the class I and II ncRNAs . Identification of the DUSE motif ([AT]CCCA[AT]AA) in D. purpureum revealed that a DUSE also sits upstream of all D. purpureum spliceosomal RNA genes. The DUSE also enriches for a family of putative D. purpureum ncRNAs that are homologous to the two novel classes of D. discoideum ncRNAs. This suggests that the DUSE is not specific to D. discoideum.
To identify putative homologs to the class I and II ncRNAs in D. purpureum, we used the structural characteristics of these ncRNAs to filter all sequences containing the DUSE-enriched 8-mers. Forty members of the class I and II ncRNAs were originally identified in D. discoideum. Some are described as putative, with nine lacking the canonical bulge sequence, and five others lacking an upstream DUSE, or having a degenerate DUSE. The class I ncRNAs have a 5' stem sequence of GTTGA, while two class II ncRNAs have a 5' stem sequence of GCTCG, and all members have a 3' stem sequence complementary to the 5' stem sitting 40 to 70 bp away from the 5' stem .
In our analysis of the masked D. discoideum genome, we identified 46 occurrences of the CTTACAGC 8-mer (Additional file 1). Of these, 26 possess both an upstream DUSE and a 5'/3' stem pair sitting 40 to 70 bp apart, and each corresponds to a previously identified class I or II ncRNA. In the masked D. purpureum genome there are 61 occurrences of the CCTTACAG 8-mer; 26 of these 8-mers have both an upstream DUSE and a 5'/3' stem pair consisting of an identical 5' sequence (GAATT) (Figure 4). These results suggest a class of ncRNAs in D. purpureum similar to the class I and II ncRNAs found in D. discoideum.
The comparative genomics approach to identifying these ncRNAs in D. purpureum lends deeper insight into their function. The 5' and 3' stem sequences have diverged between species, but have done so in a compensatory manner that maintains the predicted 5'/3' structure. The NSBR sequence, however, has remained perfectly conserved between species, and in neither species is it predicted to self-bind. This suggests a functional role for the NSBR beyond self-interaction, possibly as a binding site for another functional element. Initial genomic analysis of the dictyostelids Dictyostelium citrinum and Polysphondylium violaceum also revealed putative ncRNAs with an upstream DUSE, the conserved NSBR sequence, a 5'/3' stem structure, but 5'/3' stem sequences different from those of D. discoideum and D. purpureum (unpublished data).
Determination of protein orthologs
Of the 12,410 predicted D. purpureum proteins, we identified 7,619 that are likely to be orthologous to D. discoideum proteins using the Inparanoid algorithm, best reciprocal blast hits, and manual curation (Additional file 3). An additional 2,759 predicted proteins are similar to genes in D. discoideum, while 2,001 appear to be unique to D. purpureum (Additional file 4). Thus, at least 84% of the protein-coding genes in D. purpureum share orthologs or paralogs in the D. discoideum genome. The gene product predictions from the D. purpureum genome should be enormously useful for further refinement of the predicted proteome of D. discoideum. Some gene families are completely conserved between D. purpureum and D. discoideum, with clear orthologs for every member of the family, while other families appear to have undergone considerable divergence between the two species (Figure S9 in Additional file 1, and Additional file 4). The differences amongst gene family members should illuminate the physiological differences between these two dictyostelids, whereas the similarities may indicate where the selective pressures, exerted by their common environment, have resulted in stable gene inventories required for survival.
Polyketide synthases (PKSs) are enzymatic production lines for making small molecules by the repeated condensation of malonyl-CoA and other thio-esters of coenzyme A (CoA). A large number of polyketides exist and are probably made for ecological purposes, but they also serve as model natural products for the development of drugs, antibiotics and food additives. Soil amoebae are not commonly regarded as polyketide producers, but they too must face complex ecological challenges, which could be met by polyketide production; competition from other amoebae, infection by bacteria and predation by nematodes, amoebae and fungi. A small number of potential eco-chemicals have been identified from social amoebae [32, 33], but the completed D. discoideum genome sequence revealed a much larger potential [1, 34, 35]. These PKSs are large, modular proteins of 2,000 to 3,500 amino acids, each having a core of domains for the condensation reaction, together with optional domains for methylation, carbonyl reduction and product release. Two have a unique, 'steely', architecture in which a second PKS - a chalcone synthase - is fused to the carboxyl terminus of a modular PKS . One of these steely proteins makes the precursor of differentiation-inducing factor (DIF)-1, a chlorinated signal molecule for stalk cell differentiation , and the other a pyrone or an olivetol derivative [35, 36, 38].
In contrast to the four D. purpureum genes described above, most D. purpureum PKS genes do not have obvious orthologs in D. discoideum, indicating species-specific expansions. Given the overall gene conservation between these two species, the divergence of the PKS gene sets is striking. We speculate that this greater evolutionary fluidity reflects different selective pressures placed on the two species, perhaps by different competitor species in their ecological niches, and therefore that most of their polyketides are produced for ecological purposes.
The D. purpureum genome confirms the high potential of social amoebae for polyketide production. The relative paucity of orthologs to D. discoideum PKSs raises the possibility that polyketide production varies substantially from species to species amongst the dictyostelids. As natural products remain the major source of drugs , this diversity suggests that natural products of social amoebae deserve systematic exploration.
The ATP-binding cassette transporters
The ABC transporters are one of the largest protein superfamilies that are encoded by any genome. In stark contrast to the lineage-specific radiation of the PKS proteins, the complement of ABC transporters has remained remarkably stable since the divergence of D. purpureum and D. discoideum. ABC proteins all have a conserved domain of 200 to 250 amino acids, the ATP-binding cassette, and typically have 12 transmembrane domains. Seven different eukaryotic families have been defined on the basis of sequence homology, domain topology and function. The superfamily has been extensively analyzed in D. discoideum  and this allowed a detailed comparison to the predicted D. purpureum ABC superfamily members. Both genomes carry similar numbers of ABC genes overall, but differences in gene number can be observed within groups of closely related genes belonging to the largest families (Tables S7 and S8 in Additional file 1). Only 58 genes can be considered clear orthologs; the remaining genes should be considered paralogs (Figure S10 in Additional file 1). These genes may play partially redundant roles and this might allow their sequences to drift to a point of uncertain orthology.
The Tag subfamily proteins (TagA-D) of the ABC B family have a novel domain structure with a serine protease domain on the amino terminus, a single set of six transmembrane domains, and one ABC domain on the carboxyl terminus. Three of the Tag proteins have defined roles in cell differentiation; TagA is involved in early cell fate determination , TagB is required for pre-stalk cell differentiation , and TagC is expressed in pre-stalk cells and required to process acyl-CoA binding protein into a spore differentiation peptide signal . Interestingly, TagA, B and C are conserved between D. purpureum and D. discoideum, but whereas the TagA orthologs are quite similar, the relationship between the TagB and TagC proteins in the two species is not as clear (they were named based on their gene order within a block of synteny between D. discoideum and D. purpureum).
D. purpureum has a similar complement of protein kinases compared to D. discoideum. Like D. discoideum, D. purpureum does not appear to have receptor tyrosine kinases, or other notable protein kinases such as P70, ATM, and PASK. There are 262 eukaryotic protein kinases and 41 atypical protein kinases, including potential pseudogenes (Table S9 in Additional file 1). This compares to 247 identified eukaryotic protein kinases and 39 atypical protein kinases in D. discoideum .
The 14 D. purpureum histidine kinase genes, and the related acrA gene, each have an unambiguous ortholog in D discoideum (Figure 5b). There is little homology between non-orthologous genes outside of the kinase domain. Thus, the histidine kinases appear to have diverged from a common ancestor before the radiation of the dictyostelids, suggesting that each one of them carries out a distinct and conserved function. The adenylyl cyclase of D. discodeum, AcrA, carries a non-functional histidine kinase domain with mutations in key amino acids that preclude kinase activity . This domain and its variations are well conserved in the D. purpureum AcrA, suggesting that there is a selective advantage to maintaining this non-catalytic domain, probably as a dimerization domain.
The catalytic subunit of cAMP dependent protein kinase (PKA), PkaC, in D. purpureum shows 65% amino acid identity with its D. discoideum ortholog. The homology is highest in the catalytic core and lowest in the low complexity amino-terminal domain, with the exception of the region encompassing the αA amphipathic helix . This helix, which is predicted to interact with a hydrophobic pocket on the catalytic core of the enzyme, is 95% identical in these dictyostelids, which is suggestive of a conserved regulatory function. The regulatory subunit of PKA, PkaR, of D. purpureum and D. discoideum shows 79% amino acid identity and each of them lack the dimerization domain found in metazoa.
G-protein coupled receptors
GPCRs are found in all eukaryotes and transduce a variety of extracellular signals via heterotrimeric G-proteins and effector proteins inside the cell to elicit physiological responses. GPCRs are characterized by an extracellular domain, an intracellular domain, and a core domain that contains seven transmembrane regions. The GPCRs are subdivided into six major families that, aside from their conserved secondary domain structure, do not share significant sequence similarity. The D. purpureum genome encodes the same families of GPCRs as in D. discoideum, but has a reduced total number, which is mainly due to differences in the numbers of cAMP, family 3 and family 5 receptors (Figure S12 and Table S10 in Additional file 1). There are only two cAMP receptors in the D. purpureum genome, namely orthologs of Dictyostelium carA and carB, but there are no orthologs of carC and carD. In addition, there are 35% fewer family 3 receptors and 40% fewer family 5 receptors. This difference must be due either to an expansion of family 3, 5 and cAR receptors in D. discoideum or to a reduction in the D. purpureum genome. Either D. discoideum has evolved many new functions for GPCRs compared to D. purpureum or else there is more functional overlap amongst the D. discoideum receptors.
The overall comparison of transcription factors in D. discoideum and D. purpureum shows gross conservation both in the total number of genes in each family, and at the protein sequence level (Table S11 in Additional file 1). There are only 11 basic leucine zipper (bZIP) domains in D. purpureum, versus 19 in D. discoideum. Among the 11 bZIPs found in both species are DimA and DimB, which are involved in DIF signaling in D. discoideum, as well as bZIP candidates for CREB and GCN4, which are the most conserved bZIPs among eukaryotes (E. Huang, M. Katoh-Kurasawa and G. Shaulsky; unpublished). There are an equal number of STAT transcription factors in D. purpureum and D. discoideum (four), each with a high degree of protein sequence identity. In the original description of the D. discoideum genome, the paucity of transcription factors was noted . One explanation for the small number of recognized transcription factors was the possibility of new classes of transcription factors that evade conventional detection based on sequence searches. One example is the recently defined CudA nuclear protein that binds in vivo to the promoter of the cotC prespore gene . CudA-related proteins have recently been defined as being specific to the amoebozoa , but there are distantly related proteins in plants .
The actin cytoskeleton and its regulation
The D. purpureum repertoire of microfilament system proteins is almost an exact replica of that described in D. discoideum (Table S12 in Additional file 1) . In contrast, the actin-depolymerizing factor (ADF) protein family differs between the Dictyostelium species. A phylogenetic tree of all ADF domains encoded by the genomes of both species shows three major groups (Figure S13 in Additional file 1). The ADF domains present in cofilin, twinfilin and GMF (glia maturation factor) constitute one group. D. purpureum has two genes encoding cofilins, cofA and cofG. Only cofA has a direct ortholog amongst the eight D. discoideum genes. An additional group of ADF domains is present in D. purpureum that includes three proteins, one of which (DPU_G0064410) has no direct ortholog in D. discoideum and another (DPU_G0060306) that is related to two D. discoideum genes (DDB_G0270134 and DDB_G0270132).
A family of proteins where there has been some expansion in D. purpureum is that of the I/LWEQ domain-containing proteins. Besides two talins and a single Sla2/HIP1, D. purpureum harbors three more genes related to hipA encoding only a carboxy-terminal fragment that encompasses the I/LWEQ domain. It is not clear whether these are actually pseudogenes. Similarly, we have found a group of at least eight genes that encode short proteins related to the carboxy-terminal part of HIP1 immediately upstream of the I/LWEQ domain. The extensive family of calponin homology (CH) domain proteins in D. purpureum has two members absent in D. discoideum. One (DPU_G0069574) is related to conventional fimbrins but lacks EF hands and has a weakly conserved fourth CH domain. The other (DPU_G0074288) is a protein with a carboxy-terminal CH domain.
Cytoskeletal remodeling during chemotaxis and phagocytosis is regulated by a considerable number of upstream signaling components. Especially important are those components involved in signaling to and from small GTPases of the Rho family, as recently described in D. discoideum . In general terms the repertoire of genes encoding proteins that participate in Rho signaling is very similar in both dictyostelid species, with some exceptions (Tables S13 and S14 in Additional file 1). The Rho GTPase family itself has diversified considerably in D. purpureum and D. discoideum (Figure S14 in Additional file 1). This family currently comprises 20 rac genes and one pseudogene in D. discoideum and 18 genes in D. purpureum. Most D. discoideum rac genes have a direct ortholog in D. purpureum, but the degree of conservation is variable. There is a second rac1a-related gene, indicating that the ancestral rac1 gene duplicated independently in each organism. There is no ortholog for D. discoideum rac1b, rac1c, racF1, racF2, racI and racM to racO, and the pseudogene racK and, conversely, D. purpureum has five more rac genes without a D. discoideum counterpart (racR to racW), again indicating that the rac family has undergone independent divergence in both species.
Among the Rho regulators D. purpureum appears to have one RhoGAP gene less than D. discoideum. The missing RhoGAP gene is gacII; the corresponding protein consists of a RhoGAP domain followed by a SH3 domain. The protein is very similar to the amino-terminal half of RacGAP1 (xacA gene), suggesting that gacII resulted from a partial duplication of xacA in D. discoideum. Among the Rho effectors, the class PI4P 5 kinases have undergone a notable expansion in D. purpureum (Table S14 in Additional file 1). Additional descriptions of Ras superfamily members can be found in Additional file 1.
The D. purpureumglycome
Glycosylation is an extensive post-translational modification of proteins, and also occurs on lipids, nucleic acids and, of course, polysaccharides, in all forms of life. Though basic glycosylation pathways tend to be conserved among eukaryotes, glycosylation details can vary between species and cell types, and even between individual proteins as 'microheterogeneities'. In D. discoideum, protein glycosylation has been implicated in protein sorting and stability, cell proliferation, adhesion and sorting, spore coat assembly, resistance to cisplatin, and oxygen signaling. The inventory of predicted glycogenes likely to be associated with both anabolic and catabolic aspects of glycan metabolism approaches 2.5% of the genome (Tables S16, S17, S18, and S19 in Additional file 1), typical for metazoans but lower than for higher plants. As discussed below, a comparison of D. purpureum with the previously annotated glycogenes of D. discoideum , in the context of the global CAZy classification [55, 56], suggests examples of both considerable conservation and diversification of their glycomes.
Protein N-glycosylation, the most prevalent and highly conserved type of protein glycosylation, is initiated in the rough endoplasmic reticulum of D. discoideum by the transfer of a 14-sugar chain from a lipid-linked precursor  that is identical to the yeast and human precursor but distinct from that of many protists . Maturation of the sugar chain leads to a preponderance of high-mannose glycans with bisecting and novel intersecting β-linked GlcNAc, and α3-linked core fucose characteristic of plants and invertebrates, followed by increased α-mannosidase processing during development [59, 60]. D. discoideum N-glycans are often rendered anionic by phosphorylation and sulfation [57, 59], in contrast to the typical sialic acid or uronic acid modifications of animal glycans.
A genomic comparison suggests that the N-glycome of D. purpureum will be similar to that of D. discoideum but with some interesting differences (Table S16 in Additional file 1). For example, putative CAZy GT49 β3-GlcNAc transferases, GT10 α3/4-fucosyltransferases, and glycophosphotransferases, expected to mediate peripheral modifications of N-linked and perhaps other glycans, are represented by much smaller gene families in D. purpureum, and low amino acid sequence similarities make ortholog predictions for individual family members less certain. Thus, D. purpureum may exhibit reduced prevalence and diversity of its peripheral glycan modifications.
Other glycosylation events associated with the secretory pathway
A previous inspection of the predicted D. discoideum proteome also indicated the existence of some major classes of biosynthetic enzymes associated with mucin-type O-glycans, O-phosphoglycans, and glycosylphosphatidylinisotol (GPI) anchors , in agreement with biochemical studies . For example, mucin-type O-glycosylation is initiated in the Golgi by a CAZy GT60 polypeptide α-GlcNAc transferase, conserved in both dictyostelids and related to the polypeptide α-GalNAc transferases associated with mucin-type O-glycosylation in animals . Glycophosphorylation of the hydroxyamino acids threonine and serine may be less prevalent in D. purpureum owing to the much smaller size of its glycophosphotransferase-like gene family (Table S16). Although the glycogene comparison suggests a general conservation of these other aspects of the glycome, differences suggest that there may be equally dramatic variations as observed for N-glycosylation.
Whereas glycosylation occurs predominantly in the secretory compartments, formation of the precursors for these pathways generally originates in the cytoplasm, and the cytoplasm is also a site for catabolic deglycosylation. The genome encodes proteins associated with these functions as expected. In addition, like most eukaryotes, D. discoideum encodes a potential nucleo-cytoplasmic Spy-like GT41 O-GlcNAc transferase (OGT or Ser/Thr-βGlcNAc transferase) and D. purpureum encodes two; the physiological function(s) of O-GlcNAc in protists is currently unknown . D. discoideum also possesses a complex cytoplasmic O-glycosylation pathway that modifies hydroxyproline and has an ancient evolutionary relationship with O-glycosylation in the secretory pathway and bacterial glycosylation . The genes of this pathway are highly conserved in D. purpureum, and bioinformatics and biochemical data indicate its partial conservation across at least four major protist phyla. This pathway is devoted to the modification of the E3 ubiquitin ligase subunit Skp1, and is involved in oxygen regulation of development in D. discoideum .
Carbohydrate binding proteins
Many glycan functions are mediated in trans via carbohydrate binding domains (CBDs) or lectins. During initial remodeling within the rough endoplasmic reticulum, N-glycans are recognized by lectins in a folding/quality control cycle and, unlike many protists, this pathway appears to be highly conserved between the dictyostelids and animals . D. discoideum encodes numerous cytoplasmically localized lectins, including multiple discoidin, Cup and comitin proteins [67–69], and glycogen-binding proteins involved in metabolic regulation (Tables S18 and S19 in Additional file 1). Except for the latter, the natural glycan ligands in the cytoplasm are unknown. Interestingly, discoidins, like galectins of animals, exit cells via a non-classical process and potentially bind self, prey or predator glycans containing Gal or GalNAc . Discoidin and Cup CBDs appear to be dictyostelid-specific and evolutionarily dynamic, suggesting they serve species-specific functions as suggested for other lineage-specific expansions .
Both genomes encode a few more glycohydrolases (Table S16 in Additional file 1) than glycosyltransferases, with suspected substrates ranging from dietary polysaccharides and glycans of bacterial, yeast and perhaps other prey to endogenous glycans for recycling. Potentially 11 of the glycohydrolase domains are fused to carbohydrate binding modules (CBMs), a subset of CBDs associated with enzymes. As described for some cellulases such as CelA, the CBM may localize the enzyme to the target substrate after secretion, and may also directly promote catalysis . Peptidases may be localized at the cell surface by a similar mechanism. Cellulases are likely to be involved in remodeling of slime sheath cellulose during morphogenesis and spore coat breakdown during germination. D. purpureum and D. discoideum also have a cellulase associated with extracellular digestion in fungi and other cellulose-digesting organisms (CAZy GH7), suggesting a similar role in the social amoebae. Though the number of glycosyltransferases and known glycan binding proteins is 10 to 20% smaller in D. purpureum than D. discoideum, correlating with fewer peripheral modifications, the number of potential glycohydrolases is approximately 10% greater. The latter differences occur in lysozyme-, chitinase-, and alpha-mannosidase-like enzymes, suggesting variation in the spectrum of bacterial and yeast prey between the species.
Multicellular development and dictyostelid sociality
The dictyostelid social amoebae undergo multicellular development when nutrients become limiting for vegetative growth. The ensuing events of aggregation of individual cells into an initial mound, slug migration, and ultimately fruiting body morphogenesis, require several cooperative interactions between the cells. These cooperative cellular behaviors include: cellular chemotaxis to self-generated, field-wide spiral waves of extracellular cAMP; the coordinated movements of cells within specialized tissues of the mounds and slugs requiring differential cell adhesion; an innate immune system; and the apparent altruism displayed by the pre-stalk cells that die as they construct the stalk, presumably to aid the dispersal of the spores in the sorus. The initial analyses of the D. discoideum genome uncovered a number of protein classes that might mediate this extensive cellular cooperation, and that were previously thought to be unique to metazoa . These proteins included certain subfamilies of ABC transporters, metabotropic GPCRs, and cell surface proteins predicted to contain repeated epidermal growth factor (EGF) or Ig-like domains that had not previously been seen in plants, fungi or amoebae.
One large family of 37 metazoan-like proteins described in D. discoideum, the Tiger (transmembrane, IPT, Ig-like, E-SET repeat) proteins, contain family members that mediate cell-cell interactions during development. Mutations in tgrB1, tgrC1 (formerly lagC), tgrD1 and tgrE1 all result in the arrest of development at the mound stage, and TgrB1 and TgrC1 have been implicated in a self/non-self recognition system that may mediate kin recognition . Twenty-six Tiger-protein encoding genes are present in the D. purpureum genome, including orthologs to D. discoideum's tgrC1, tgrD1, tgrF1, tgrK1, tgrM2, and tgrN1 genes. Their presence suggests that the Tiger protein family may be generally involved in allorecognition in the dictyostelids.
An innate immune system has been recently described that functions during slug migration of D. discoideum and appears to be present in other group 4 dictyostelids, including D. purpureum . It consists of a population of sentinel cells that patrol the body of the slug, engulf any bacteria that are present, bind to the slime sheath, and then exit the slug by being left behind in the slime trail. Sentinel cells are 1% of all slug cells and express particular genes that are related to innate immunity signaling genes in plants and animals, such as slrA and tirA. In particular, tirA, a Toll/interleukin receptor I domain containing protein, is required for some aspects of sentinel cell function . D. purpureum has orthologs for both slrA and tirA, as well as three amoeba-specific lysozymes (Additional file 4).
Cyclic nucleotide signalling genes
cAMP controls many aspects of dictyostelid development. As a dynamically secreted chemoattractant it directs the cell movement that causes cells to aggregate, and aggregates to transform into fruiting structures. Secreted cAMP also triggers pre-spore differentiation, up-regulates the expression of aggregation genes and down-regulates stalk gene expression. As an intracellular messenger for other stimuli, cAMP induces spore and stalk encapsulation and maintains spore dormancy [23, 46, 75, 76].
The cNMP binding domains are found in the regulatory subunit of PKA (PkaR), the cGMP binding proteins GbpC and GbpD and the phosphodiesterases (PDEs) PdeD and PdeE. PdeD is a cGMP phosphodiesterase that is stimulated by cGMP binding to its cNMP binding domains, while PdeE is a cAMP-stimulated cAMP phosphodiesterase [76, 80]. GbpC is a complex multidomain protein in which cGMP binding to its cNMP binding domains sequentially activates the intrinsic RasGEF, Ras/Roc and protein kinase domain, which eventually leads to increased cell polarization. GbpD also contains a RasGEF domain, but no output protein kinase domain. Its cNMP binding domains are not functional and it functions as an antagonist of GbpC in the chemotactic response [81, 82]. Genes encoding all five cNMP binding proteins with their complete sets of functional domains are present in the D. purpureum genome (Figure 7c).
In D. discoideum cyclic nucleotides are hydrolyzed by three structurally distinct PDEs . The cAMP PDEs RegA and Pde4 and the cGMP PDE Pde3 harbor a PDE_I type domain with HDc motif that is common to mammalian PDEs. The dual-specificity PDEs PdsA and PDE7 harbor a PDE_II type domain with HSHLDH motif. PdeD and PdeE, which hydrolyze cGMP and cAMP, respectively, carry a related HCHADHDS motif, but are structurally more similar to the lactamase_B protein family. The PDE_I and PDE_III enzymes are fully conserved between D. discoideum and D. purpureum, but the latter species has three instead of two type II PDEs (Figure 7d).
The high level of conservation between D. discoideum and D. purpureum of all adenylate and guanylate cyclases, cNMP binding domains and seven out of eight PDEs, combined with the complete conservation of functional domain architecture of these proteins, is indicative of the central roles of cAMP and cGMP in the control of chemotaxis, morphogenesis and gene regulation in the dictyostelids.
DIF is produced predominantly by pre-spore cells during D. discoideum development and is part of a signaling mechanism that sets the ratio of stalk and spore cells produced in the fruiting body. It both limits the number of pre-spore cells produced and induces differentiation of a subset of pre-stalk cells. DIF is made by a three step biosynthetic pathway, in which a 12-carbon polyketide is assembled by the StlB polyketide synthase, then successively chlorinated by a chlorinating enzyme, and methylated by the DmtA methyltransferase [36, 83, 84]. Clear stlB and dmtA homologs exist in the D. purpureum genome, as does a homologue of a recently identified FAD-dependent chlorinating enzyme (C Neumann, C Walsh and RR Kay, unpublished). DIF is inactivated by glutathione-dependent dechlorination , and again this enzyme has recently been identified and has a clear homolog in D. purpureum (F Velazquez and RR Kay, unpublished). It thus appears certain that D. purpureum makes and degrades DIF in a similar way to D. discoideum, and presumably utilizes it in a similar way to regulate multicellular development.
Dictyostelids are interesting social organisms because about 20% of the cells in each fruiting body sacrifice themselves to build the stalk. Groups form through aggregation of formerly separate cells, so different clones can aggregate together, and do so in both the lab and in the field . Clones that successfully compete to get into spores, relegating their partners to the sterile stalk, will be more successful. Some degree of conflict is therefore predicted, and the resulting evolution of strategies and counter strategies may drive rapid adaptive evolution, as appears to be true for genes involved in host-parasite conflicts and male-female conflicts . But there is also a second reason to expect that social genes may evolve more rapidly than genes expressed primarily in the solitary stage. If the social stage occurs relatively infrequently, which seems likely but is unknown, then social genes are less scrutinized by selection and could accumulate more changes through genetic drift.
We tested for more rapid evolution using two ways of defining social genes. The first was to examine the set of genes that emerged from a selection for mutants that cheat (make more than their fair share of spores in mixtures) and compare them with all other genes . The two sets do not differ significantly in the probability of having homologs, suggesting that they neither differentially disappear nor differentially evolve beyond the point of clear homology (Figure S18a in Additional file 1). The two sets also do not differ in probability of having paralogs, suggesting that they do not duplicate at different rates (Figure S18b in Additional file 1). Finally, the two sets do not differ for either dN (the rate of non-synonymous change) or conservation score (a measure that declines with both point differences and with non-aligned portions of the sequences) (Figures S18c and S19d in Additional file 1). However, this set of social genes is relatively small, and some will be false positives (of 198 genes identified, 40 were tested for cheating, of which 31 were cheaters).
A larger set of social genes can be identified using RNA-seq reads from the vegetative stage and six social time points (4, 8, 12, 16, 20, and 24 hours after starving) . Using genes with sufficient reads and high reproducibility (Additional file 1), we defined a gene's index of social expression as the average percentage representation in the social-stage libraries over that average plus the percentage representation in vegetative stage (that is, Social expression/Social expression + Vegetative expression).
Using this classification, social genes showed higher rates of change, and manifested fewer orthologs, higher rates of non-synonymous substitution, and lower conservation scores. Genes with orthologs in D. discoideum and D. purpureum have a significantly lower social expression index in D. discoideum than those without orthologs (Figure S19a in Additional file 1; n = 1,739, 1,300, P < 2.2e-16, Mann-Whitney U test). This is driven by significant differences in each time point of the developmental stages (data not shown). An analysis using genes with D. purpureum RNA-seq data following the above criteria gives a similar overall result (Figure S19b in Additional file 1; n = 3,649, 2,102, P < 2.2e-16, Mann-Whitney U test). Thus, genes with more social expression are less likely to have orthologs, indicating more rapid evolution in the gain or loss of genes, or in change of genes beyond the point where they are identifiable as homologs. Homologs that had inparalogs show no significant difference of social indices from those that lacked inparalogs when the social expression is measured with RNA-seq reads from D. discoideum (Figure S19c in Additional file 1; n = 95, 1,644, P = 0.93, Mann-Whitney U test) and D. purpureum (Figure S19d in Additional file 1; n = 137, 3,512, P = 0.24, Mann-Whitney U test), suggesting no significant bias of duplication for the social genes of dictyostelids.
Dictyostelium has a sexual cycle in which two cells fuse and then engulf many other cells to form a giant macrocyst that undergoes meiosis. However, with the exception of one successful cross , the sexual system has not been available in lab studies of D. discoideum. Although macrocysts are readily formed, there are problems with germination  and, when there is germination, there may be no recombinants . Finding the right conditions for sex would add a valuable genetic dimension to D. discoideum studies, but this search would be fruitless if most strains have lost the ability to have sex. If they have lost this ability, we would expect that sex-specific genes would have degraded. We tested this hypothesis using ESTs from gamete-stage libraries made from cells grown in conditions that make them competent for fusion . Figure 8c shows that genes expressed disproportionately in the gamete stage are actually more conserved than other genes, as measured by both dN and conservation score. Provided these truly are sex-specific genes, then it appears that the macrocyst system is functional and not degenerating in D. discoideum. This is supported by an analysis showing that 13 meiosis genes  have normal dN and conservation score values compared to other genes (Figure S21 in Additional file 1).
Comparisons of the D. purpureum genome, the second group 4 dictyostelid to be sequenced, with the previously sequenced D. discoideum have provided insights into the evolution of this clade of social amoebae. Like D. discoideum, the genome of D. purpureum encodes a high number of triplet nucleotide repeats distributed in both exonic and non-protein-coding regions. However, these tracts are not generally congruent between the two genomes, indicating that their expansion is a consequence of an intrinsic physiology favoring high rates of triplet repeat formation, rather than retention and accumulation of ancient triplet repeats. Although the D. purpureum genome was not finished to the same extent as the D. discoideum genome, syntenic regions containing orthologous genes are detected. Genes that have been hypothesized as having been acquired through horizontal gene transfer in the D. discoideum genome have orthologs in the D. purpureum genome; thus, any HGT events involving these genes likely occurred in the common ancestor of the group 4 dictyostelids. Likewise, large gene families of ABC transporters and histidine kinases underwent expansion in the common ancestor before the species line split, while the expansion of polyketide synthase genes occurred in a lineage-specific manner. The repertoire of microfilament system proteins is virtually identical between the two species, but the regulatory proteins differ. Two distinct transferases involved in the N-linked glycosylation were detected in D. discoideum but not in D. purpureum, and the predicted change in the glycosylation state of the respective species proteins was validated through glycomic analysis. Comparative analyses also enabled the identification of two novel classes of ncRNAs specific to the dictyostelid lineage. High conservation of enzymes involved in cNMP metabolism and DIF production and degradation indicate the central role these signaling systems play in the social behavior of these amoebozoa. A detailed comparison of the variation between cohorts of genes with specific expression patterns between the two genomes demonstrate that genes involved in sociality evolve more rapidly, probably due to continuous adaptation and counter-adaptation.
Materials and methods
Sequence and assembly
D. purpureum was described in 1902 by Olive . D. purpureum isolate QSDP1 from the Queller and Strassmann laboratories at Rice University, and its axenic derivative DpAX1, were used in this study. DpAX1 was selected from QSDP1 for the ability to grow axenically, in defined liquid media, by culturing in plastic Petri dishes containing HL5 medium supplemented with 10% fetal bovine serum .
QSDP1 was used for EST production and sequencing. Cells were grown in association with Klebsiella pneumoniae, harvested and developed on nitrocellulose filters as described . RNA samples were prepared from developing cells at 0, 6, 12, and 18 hours . Two cDNA libraries were prepared from each of these four RNA samples and a total of 14,949 validated EST clones were sequenced from them. Briefly, polyA-selected RNA was reverse transcribed with superscript reverse transcriptase III (Invitrogen, Carlsbad, CA, USA) using dT primer (5' GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTTVN-3'). cDNA was synthesized with Escherichia coli DNA polymerase I, E. coli DNA ligase, and E. coli RNaseH. The DNA ends were repaired with T4 DNA polymerase. SalI adapters (5'-TCGACCCACGCGTCCG-3' and 5'-P04-CGGACGCGTGGG-3') were ligated to cDNA and the product was digested with NotI. The cDNA digestion products were gel purified and directionally ligated into SalI- and NotI-digested pCMVsport6 and transformed into ElectroMAX, T1 DH10B E. coli cells (Invitrogen). Plasmid DNA was amplified by a rolling circle method (Templiphi, GE Healthcare, Piscataway, NJ, USA) and purified. The insert of each clone was sequenced from both ends with primers complementary to flanking vector sequences using Big Dye terminator chemistry and resolved by an ABI 3730 sequenator (ABI, Foster City, CA, USA).
To prepare high quality genomic DNA, DpAX1 cells were grown in shaking cultures in HL5 medium, and DNA was prepared from isolated nuclei by cesium chloride equilibrium density gradients. Genomic libraries were constructed by shearing genomic DNA with a Hydroshear (Genomic Solutions Inc., Ann Arbor, MI, USA) to create 6- to 10-kb fragments. The DNA fragments were size selected, purified, blunt-end repaired (End-It Kit, Epicentre Biotechnologies Madison, WI, USA) and ligated into a pMCL200 vector. The ligation product was purified and precipitated, and then transformed into ElectroMax DH10B competent cells (Invitrogen). The percentage of no-insert clones in the library was assessed by colony PCR, using primers flanking the cloning site (Expand long Template PCR system, Roche Applied Science, Indianapolis, IND, USA). Genomic DNA libraries with average insert sizes of 2.3 to 3.0 kb and 3 to 4 kb were produced by similar methods.
Primary sequence data were derived from whole-genome shotgun sequencing of the three plasmid libraries . The reads were screened for vector sequence with cross_match  and trimmed for vector and low quality sequences. Reads shorter than 100 bases after trimming were excluded from the assembly. The trimmed read sequence data were assembled with release 1.0.3 of Jazz, a whole genome shotgun assembler . The assembly was next filtered for redundant scaffolds that matched larger scaffolds (<5 kb length where >80% matched a scaffold of >5 kb length). Finally, scaffolds that showed homology to prokaryotic and non-cellular contaminants (viroids and viruses) were identified and removed. The filtered assembly contains 799 scaffolds, comprising 33.0 Mb, with an estimated sequence coverage of 8.41 × (Additional file 1). The data were deposited in GenBank under project ID 30991 [GenBank:ADID00000000].
The JGI genome annotation pipeline
For genome annotation we use the JGI annotation pipeline, which combines several gene prediction, annotation and analysis tools. First, the genome assembly is masked using RepeatMasker and a custom repeat library. Next, available ESTs and full-length cDNAs are clustered and aligned to the scaffolds with BLAT. Model organism protein sequences from the non-redundant set of proteins from the National Center for Biotechnology Information (GenBank) are aligned to the scaffolds with BLASTX . Gene models and associated transcripts/proteins are predicted or mapped using (i) data from putative full-length cDNAs derived from available mRNA, ESTs and EST clusters, (ii) homology-based methods Genewise and Fgenesh+, and (iii) ab initio method Fgenesh trained on putative full-length genes (see above), manually curated genes (if available), and reliable homology-based models. Additional gene models generated externally with other gene predictors trained for a particular genome can be added as well. The clustered ESTs/cDNAs are used to extend and correct predicted gene models where the exons overlap and splice junctions are not consistent in comparing EST sequences to gene models. This often adds 5' and/or 3' UTRs to the models. With gene structure in place, function is assigned to models based on Smith-Waterman homology to annotated genes from nr, KEGG, and KOG databases. InterproScan is used to identify predicted domains and the Gene Ontology is used to identify function and/or subcellular location. SignalP is used to assist with identification of secreted proteins. Since multiple models with overlapping sequences are generated for each locus, a single model is chosen to produce a non-redundant set of genes. Model selection is based on homology to known proteins from other organisms, EST support, as well as protein and transcript completeness (that is, inclusion of 5' methionine, 3' stop codon, and UTRs). This automatically generated set was further refined by manual curation and submitted to GenBank. Whole genome analysis is performed on the non-redundant set of gene models or a snapshot of a manually curated gene catalog assuming the latter includes significant number of changes compared to the automatically generated non-redundant set.
basic leucine zipper
carbohydrate binding domain
carbohydrate binding module
cyclic nucleoside monophosphate
Dictyostelium upstream sequence element
expressed sequence tag
G-protein coupled receptor
horizontal gene transfer
non self-binding region
cAMP dependent protein kinase
This work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program and the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344, Los Alamos National Laboratory under contract No. DE-AC02-06NA25396. The annotation effort was supported by grants HD39691 (A Kuspa, GS, and RS), GM64426 (RLC), HG0022 (RLC) and GM84383 (CMW) from the National Institute of Health, and by grants EF-0626963 and DEB-0918931 (JES and DCQ) from the National Science Foundation. AP was supported by a fellowship from the Keck Center for Interdisciplinary Bioscience Training of the Gulf Coast Consortia (NIH Grants 1 T90 DA022885 and 1 R90 DA023418).
- Eichinger L, Pachebat JA, Glöckner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, et al: The genome of the social amoeba Dictyostelium discoideum. Nature. 2005, 435: 43-57. 10.1038/nature03481.PubMedPubMed CentralGoogle Scholar
- Cavalier-Smith T: A revised six-kingdom system of life. Biol Rev Camb Philos Soc. 1998, 73: 203-266. 10.1017/S0006323198005167.PubMedGoogle Scholar
- Bapteste E, Brinkmann H, Lee JA, Moore DV, Sensen CW, Gordon P, Durufle L, Gaasterland T, Lopez P, Muller M, Philippe H: The analysis of 100 genes supports the grouping of three highly divergent amoebae: Dictyostelium, Entamoeba, and Mastigamoeba. Proc Natl Acad Sci USA. 2002, 99: 1414-1419. 10.1073/pnas.032662799.PubMedPubMed CentralGoogle Scholar
- Fiore-Donno AM, Berney C, Pawlowski J, Baldauf SL: Higher-order phylogeny of plasmodial slime molds (Myxogastria) based on elongation factor 1-A and small subunit rRNA gene sequences. J Eukaryot Microbiol. 2005, 52: 201-210. 10.1111/j.1550-7408.2005.00032.x.PubMedGoogle Scholar
- Minge MA, Silberman JD, Orr RJ, Cavalier-Smith T, Shalchian-Tabrizi K, Burki F, Skjaeveland A, Jakobsen KS: Evolutionary position of breviate amoebae and the primary eukaryote divergence. Proc Biol Sci. 2009, 276: 597-604. 10.1098/rspb.2008.1358.PubMedGoogle Scholar
- Fiore-Donno AM, Nikolaev SI, Nelson M, Pawlowski J, Cavalier-Smith T, Baldauf SL: Deep phylogeny and evolution of slime moulds (Mycetozoa). Protist. 2010, 161: 55-70. 10.1016/j.protis.2009.05.002.PubMedGoogle Scholar
- Schaap P, Winckler T, Nelson M, Alvarez-Curto E, Elgie B, Hagiwara H, Cavender J, Milano-Curto A, Rozen DE, Dingermann T, Mutzel R, Baldauf SL: Molecular phylogeny and evolution of morphology in the social amoebas. Science. 2006, 314: 661-663. 10.1126/science.1130670.PubMedPubMed CentralGoogle Scholar
- Raper KB: The Dictyostelids. 1984, Princeton, NJ: Princeton University PressGoogle Scholar
- Raper KB, Thom C: Interspecific mixtures in the Dictyosteliaceae. Am J Bot. 1941, 28: 69-78. 10.2307/2437063.Google Scholar
- Mehdiabadi NJ, Jack CN, Farnham TT, Platt TG, Kalla SE, Shaulsky G, Queller DC, Strassmann JE: Social evolution: kin preference in a social microbe. Nature. 2006, 442: 881-882. 10.1038/442881a.PubMedGoogle Scholar
- Ostrowski EA, Katoh M, Shaulsky G, Queller DC, Strassmann JE: Kin discrimination increases with genetic distance in a social amoeba. PLoS Biol. 2008, 6: e287-10.1371/journal.pbio.0060287.PubMedPubMed CentralGoogle Scholar
- Loomis WF, Kuspa A: Dictyostelium Genomics. 2005, Norfolk: Horizon Bioscience, 1Google Scholar
- JGI Dictyostelium purpureum genome portal. [http://genome.jgi-psf.org/Dicpu1/Dicpu1.home.html]
- dictyBase. [http://dictybase.org/]
- Parikh A, Miranda ER, Katoh-Kurasawa M, Fuller D, Rot G, Zagar L, Curk T, Sucgang R, Chen R, Zupan B, Loomis WF, Kuspa A, Shaulsky G: Conserved developmental transcriptomes in evolutionary divergent species. Genome Biol. 2010, 11: R35-10.1186/gb-2010-11-3-r35.PubMedPubMed CentralGoogle Scholar
- Schlotterer C: Evolutionary dynamics of microsatellite DNA. Chromosoma. 2000, 109: 365-371. 10.1007/s004120000089.PubMedGoogle Scholar
- Karlin S, Burge C: Trinucleotide repeats and long homopeptides in genes and proteins associated with nervous system disease and development. Proc Natl Acad Sci USA. 1996, 93: 1560-1565. 10.1073/pnas.93.4.1560.PubMedPubMed CentralGoogle Scholar
- Gatchel JR, Zoghbi HY: Diseases of unstable repeat expansion: mechanisms and common principles. Nat Rev Genet. 2005, 6: 743-755. 10.1038/nrg1691.PubMedGoogle Scholar
- Tian X, Strassmann JE, Queller DC: Genome nucleotide composition shapes variation in simple sequence repeats. Mol Biol Evol. 2010, 28: 899-909. 10.1093/molbev/msq266.PubMedGoogle Scholar
- Song J, Xu Q, Olsen R, Loomis W, Shaulsky G, Kuspa A, Sucgang R: Comparing the Dictyostelium and Entamoeba genomes reveals an ancient split in the Conosa lineage. PLoS Comput Biol. 2005, 1: e71-10.1371/journal.pcbi.0010071.PubMedPubMed CentralGoogle Scholar
- Myllykallio H, Lipowski G, Leduc D, Filee J, Forterre P, Liebl U: An alternative flavin-dependent mechanism for thymidylate synthesis. Science. 2002, 297: 105-107. 10.1126/science.1072113.PubMedGoogle Scholar
- NCBI. [http://www.ncbi.nlm.nih.gov/]
- Anjard C, Loomis WF: Cytokinins induce sporulation in Dictyostelium. Development. 2008, 135: 819-827. 10.1242/dev.018051.PubMedGoogle Scholar
- Yasukawa H, Kuroita T, Tamura K, Yamaguchi K: Identification of a penicillin-sensitive carboxypeptidase in the cellular slime mold Dictyostelium discoideum. Biol Pharm Bull. 2003, 26: 1018-1020. 10.1248/bpb.26.1018.PubMedGoogle Scholar
- Zhang H, Gomez-Garcia MR, Shi X, Rao NN, Kornberg A: Polyphosphate kinase 1, a conserved bacterial enzyme, in a eukaryote, Dictyostelium discoideum, with a role in cytokinesis. Proc Natl Acad Sci USA. 2007, 104: 16486-16491. 10.1073/pnas.0706847104.PubMedPubMed CentralGoogle Scholar
- Bain G, Tsang A: Disruption of the gene encoding the p34/31 polypeptides affects growth and development of Dictyostelium discoideum. Mol Gen Genet. 1991, 226: 59-64. 10.1007/BF00273587.PubMedGoogle Scholar
- Reinders Y, Schulz I, Graf R, Sickmann A: Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches. J Proteome Res. 2006, 5: 589-598. 10.1021/pr050350q.PubMedGoogle Scholar
- Putnam NH, Srivastava M, Hellsten U, Dirks B, Chapman J, Salamov A, Terry A, Shapiro H, Lindquist E, Kapitonov VV, Jurka J, Genikhovich G, Grigoriev IV, Lucas SM, Steele RE, Finnerty JR, Technau U, Martindale MQ, Rokhsar DS: Sea anemone genome reveals ancestral eumetazoan gene repertoire and genomic organization. Science. 2007, 317: 86-94. 10.1126/science.1139158.PubMedGoogle Scholar
- Hinas A, Soderbom F: Treasure hunt in an amoeba: non-coding RNAs in Dictyostelium discoideum. Curr Genet. 2007, 51: 141-159. 10.1007/s00294-006-0112-z.PubMedGoogle Scholar
- Aspegren A, Hinas A, Larsson P, Larsson A, Söderbom F: Novel non-coding RNAs in Dictyostelium discoideum and their expression during development. Nucleic Acids Res. 2004, 32: 4646-4656. 10.1093/nar/gkh804.PubMedPubMed CentralGoogle Scholar
- Hinas A, Larsson P, Avesson L, Kirsebom LA, Virtanen A, Soderbom F: Identification of the major spliceosomal RNAs in Dictyostelium discoideum reveals developmentally regulated U2 variants and polyadenylated snRNAs. Eukaryot Cell. 2006, 5: 924-934. 10.1128/EC.00065-06.PubMedPubMed CentralGoogle Scholar
- Takaya Y, Kikuchi H, Terui Y, Komiya J, Furukawa KI, Seya K, Motomura S, Ito A, Oshima Y: Novel acyl alpha-pyronoids, dictyopyrone A, B, and C, from Dictyostelium cellular slime molds. J Org Chem. 2000, 65: 985-989. 10.1021/jo991338i.PubMedGoogle Scholar
- Kikuchi H, Saito Y, Sekiya J, Okano Y, Saito M, Nakahata N, Kubohara Y, Oshima Y: Isolation and synthesis of a new aromatic compound, brefelamide, from dictyostelium cellular slime molds and its inhibitory effect on the proliferation of astrocytoma cells. J Org Chem. 2005, 70: 8854-8858. 10.1021/jo051352x.PubMedGoogle Scholar
- Zucko J, Skunca N, Curk T, Zupan B, Long PF, Cullum J, Kessin RH, Hranueli D: Polyketide synthase genes and the natural products potential of Dictyostelium discoideum. Bioinformatics. 2007, 23: 2543-2549. 10.1093/bioinformatics/btm381.PubMedGoogle Scholar
- Ghosh R, Chhabra A, Phatale PA, Samrat SK, Sharma J, Gosain A, Mohanty D, Saran S, Gokhale RS: Dissecting the functional role of polyketide synthases in Dictyostelium discoideum: biosynthesis of the differentiation regulating factor 4-methyl-5-pentylbenzene-1,3-diol. J Biol Chem. 2008, 283: 11348-11354. 10.1074/jbc.M709588200.PubMedGoogle Scholar
- Austin MB, Saito T, Bowman ME, Haydock S, Kato A, Moore BS, Kay RR, Noel JP: Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase. Nat Chem Biol. 2006, 2: 494-502. 10.1038/nchembio811.PubMedPubMed CentralGoogle Scholar
- Morris HR, Taylor GW, Masento MS, Jermyn KA, Kay RR: Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideum. Nature. 1987, 328: 811-814. 10.1038/328811a0.PubMedGoogle Scholar
- Saito T, Taylor GW, Yang JC, Neuhaus D, Stetsenko D, Kato A, Kay RR: Identification of new differentiation inducing factors from Dictyostelium discoideum. Biochim Biophys Acta. 2006, 1760: 754-761.PubMedGoogle Scholar
- Thompson CR, Kay RR: Cell-fate choice in Dictyostelium: intrinsic biases modulate sensitivity to DIF signaling. Dev Biol. 2000, 227: 56-64. 10.1006/dbio.2000.9877.PubMedGoogle Scholar
- Kay RR, Taylor GW, Jermyn KA, Traynor D: Chlorine-containing compounds produced during Dictyostelium development - detection by labelling with Cl-36. Biochem J. 1992, 281: 155-161.PubMedPubMed CentralGoogle Scholar
- Santorelli LA, Thompson CR, Villegas E, Svetz J, Dinh C, Parikh A, Sucgang R, Kuspa A, Strassmann JE, Queller DC, Shaulsky G: Facultative cheater mutants reveal the genetic complexity of cooperation in social amoebae. Nature. 2008, 451: 1107-1110. 10.1038/nature06558.PubMedGoogle Scholar
- Newman DJ, Cragg GM: Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007, 70: 461-477. 10.1021/np068054v.PubMedGoogle Scholar
- Anjard C, Loomis WF: Evolutionary analyses of ABC transporters of Dictyostelium discoideum. Eukaryot Cell. 2002, 1: 643-652. 10.1128/EC.1.4.643-652.2002.PubMedPubMed CentralGoogle Scholar
- Good JR, Cabral M, Sharma S, Yang J, Van Driessche N, Shaw CA, Shaulsky G, Kuspa A: TagA, a putative serine protease/ABC transporter of Dictyostelium that is required for cell fate determination at the onset of development. Development. 2003, 130: 2953-2965. 10.1242/dev.00523.PubMedGoogle Scholar
- Shaulsky G, Kuspa A, Loomis WF: A multidrug resistance transporter serine protease gene is required for prestalk specialization in Dictyostelium. Genes Dev. 1995, 9: 1111-1122. 10.1101/gad.9.9.1111.PubMedGoogle Scholar
- Anjard C, Loomis WF: Peptide signaling during terminal differentiation of Dictyostelium. Proc Natl Acad Sci USA. 2005, 102: 7607-7611. 10.1073/pnas.0501820102.PubMedPubMed CentralGoogle Scholar
- Goldberg JM, Manning G, Liu A, Fey P, Pilcher KE, Xu Y, Smith JL: The dictyostelium kinome - analysis of the protein kinases from a simple model organism. PLoS Genet. 2006, 2: e38-10.1371/journal.pgen.0020038.PubMedPubMed CentralGoogle Scholar
- Soderbom F, Anjard C, Iranfar N, Fuller D, Loomis WF: An adenylyl cyclase that functions during late development of Dictyostelium. Development. 1999, 126: 5463-5471.PubMedGoogle Scholar
- Veron M, Radzio-Andzelm E, Tsigelny I, Taylor S: Protein kinases share a common structural motif outside the conserved catalytic domain. Cell Mol Biol. 1994, 40: 587-596.PubMedGoogle Scholar
- Yamada Y, Wang HY, Fukuzawa M, Barton GJ, Williams JG: A new family of transcription factors. Development. 2008, 135: 3093-3101. 10.1242/dev.026377.PubMedPubMed CentralGoogle Scholar
- Watkins RF, Gray MW: Sampling gene diversity across the supergroup Amoebozoa: large EST data sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp. Protist. 2008, 159: 269-281. 10.1016/j.protis.2007.12.001.PubMedGoogle Scholar
- Rivero F, Eichinger L: The microfilament system of Dictystelium discoideum. Dictyostelium Genomics. Edited by: Loomis WF, Kuspa A. 2005, Norfolk: Horizon Bioscience, 125-172.Google Scholar
- Vlahou G, Rivero F: Rho GTPase signaling in Dictyostelium discoideum: insights from the genome. Eur J Cell Biol. 2006, 85: 947-959. 10.1016/j.ejcb.2006.04.011.PubMedGoogle Scholar
- West C, van der Wel H, Coutinho PM, Henrissat B: Glycosyltransferase genomics in Dictyostelium. Dictyostelium Genomics. Edited by: Loomis WF, Kuspa A. 2005, Norfolk: Horizon Bioscience, 235-264.Google Scholar
- CAZy: Carbohydrate-Active enZYmes Database. [http://www.cazy.org/]
- Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B: The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res. 2009, 37: D233-238. 10.1093/nar/gkn663.PubMedPubMed CentralGoogle Scholar
- Freeze HH: Dictyostelium discoideum Glycoproteins: Using a Model System for Organismic Glycobiology. 1998, ElsevierGoogle Scholar
- Samuelson J, Banerjee S, Magnelli P, Cui J, Kelleher DJ, Gilmore R, Robbins PW: The diversity of dolichol-linked precursors to Asn-linked glycans likely results from secondary loss of sets of glycosyltransferases. Proc Natl Acad Sci USA. 2005, 102: 1548-1553. 10.1073/pnas.0409460102.PubMedPubMed CentralGoogle Scholar
- Feasley CL, Johnson JM, West CM, Chia CP: Glycopeptidome of a heavily N-glycosylated cell surface glycoprotein of Dictyostelium implicated in cell adhesion. J Proteome Res. 2010, 9: 3495-510. 10.1021/pr901195c.PubMedGoogle Scholar
- Schiller B, Hykollari A, Voglmeir J, Poltl G, Hummel K, Razzazi-Fazeli E, Geyer R, Wilson IB: Development of Dictyostelium discoideum is associated with alteration of fucosylated N-glycan structures. Biochem J. 2009, 423: 41-52. 10.1042/BJ20090786.PubMedPubMed CentralGoogle Scholar
- Sternfeld J: Evidence for differential cellular adhesion as the mechanism of sorting-out of various cellular slime mold species. J Embryol Exp Morph. 1979, 53: 163-178.PubMedGoogle Scholar
- Wang F, Metcalf T, van der Wel H, West CM: Initiation of mucin-type O-glycosylation in Dictyostelium is homologous to the corresponding step in animals and is important for spore coat function. J Biol Chem. 2003, 278: 51395-51407. 10.1074/jbc.M308756200.PubMedGoogle Scholar
- Banerjee S, Robbins PW, Samuelson J: Molecular characterization of nucleocytosolic O-GlcNAc transferases of Giardia lamblia and Cryptosporidium parvum. Glycobiology. 2009, 19: 331-336. 10.1093/glycob/cwn107.PubMedPubMed CentralGoogle Scholar
- West CM, Wang ZA, van der Wel H: A cytoplasmic prolyl hydroxylation and glycosylation pathway modifies Skp1 and regulates O2-dependent development in Dictyostelium. Biochim Biophys Acta. 2010, 1800: 160-171.PubMedPubMed CentralGoogle Scholar
- West CM, van der Wel H, Wang ZA: Prolyl 4-hydroxylase-1 mediates O2 signaling during development of Dictyostelium. Development. 2007, 134: 3349-3358. 10.1242/dev.000893.PubMedGoogle Scholar
- Banerjee S, Vishwanath P, Cui J, Kelleher DJ, Gilmore R, Robbins PW, Samuelson J: The evolution of N-glycan-dependent endoplasmic reticulum quality control factors for glycoprotein folding and degradation. Proc Natl Acad Sci USA. 2007, 104: 11676-11681. 10.1073/pnas.0704862104.PubMedPubMed CentralGoogle Scholar
- Alexander S, Sydow LM, Wessels D, Soll DR: Discoidin proteins of Dictyostelium are necessary for normal cytoskeletal organization and cellular morphology during aggregation. Differentiation. 1992, 51: 149-161. 10.1111/j.1432-0436.1992.tb00691.x.PubMedGoogle Scholar
- Schreiner T, Mohrs MR, Blau-Wasser R, von Krempelhuber A, Steinert M, Schleicher M, Noegel AA: Loss of the F-actin binding and vesicle-associated protein comitin leads to a phagocytosis defect. Euk Cell. 2003, 1: 906-914. 10.1128/EC.1.6.906-914.2002.Google Scholar
- Coukell B, Li Y, Moniakis J, Cameron A: The Ca2+/calcineurin-regulated cup gene family in Dictyostelium discoideum and its possible involvement in development. Eukaryot Cell. 2004, 3: 61-71. 10.1128/EC.3.1.61-71.2004.PubMedPubMed CentralGoogle Scholar
- Aragao KS, Satre M, Imberty A, Varrot A: Structure determination of Discoidin II from Dictyostelium discoideum and carbohydrate binding properties of the lectin domain. Proteins. 2008, 73: 43-52. 10.1002/prot.22038.PubMedGoogle Scholar
- Lespinet O, Wolf YI, Koonin EV, Aravind L: The role of lineage-specific gene family expansion in the evolution of eukaryotes. Genome Res. 2002, 12: 1048-1059. 10.1101/gr.174302.PubMedPubMed CentralGoogle Scholar
- Ramalingam R, Blume JE, Ennis HL: The xi spore germination-specific cellulase is organized into functional domains. J Bacteriol. 1992, 174: 7834-7783.PubMedPubMed CentralGoogle Scholar
- Benabentos R, Hirose S, Sucgang R, Curk T, Katoh M, Ostrowski E, Strassmann JE, Queller DC, Zupan DC, Shaulsky G, Kuspa A: Polymorphic members of the lag-gene family mediate kin-discrimination in Dictyostelium. Curr Biol. 2009, 19: 567-572. 10.1016/j.cub.2009.02.037.PubMedPubMed CentralGoogle Scholar
- Chen G, Zhuchenko O, Kuspa A: Immune-like phagocyte activity in the social amoeba. Science. 2007, 317: 678-681. 10.1126/science.1143991.PubMedPubMed CentralGoogle Scholar
- Meima ME, Schaap P: Fingerprinting of adenylyl cyclase activities during Dictyostelium development indicates a dominant role for adenylyl cyclase B in terminal differentiation. Dev Biol. 1999, 212: 182-190. 10.1006/dbio.1999.9352.PubMedGoogle Scholar
- Saran S, Meima ME, Alvarez-Curto E, Weening KE, Rozen DE, Schaap P: cAMP signaling in Dictyostelium - complexity of cAMP synthesis, degradation and detection. J Muscle Res Cell Motil. 2002, 23: 793-802. 10.1023/A:1024483829878.PubMedGoogle Scholar
- Veltman DM, Keizer-Gunnik I, Van Haastert PJ: Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum. J Cell Biol. 2008, 180: 747-753. 10.1083/jcb.200709180.PubMedPubMed CentralGoogle Scholar
- Kriebel PW, Parent CA: Adenylyl cyclase expression and regulation during the differentiation of Dictyostelium discoideum. IUBMB Life. 2004, 56: 541-546. 10.1080/15216540400013887.PubMedGoogle Scholar
- Kawabe Y, Morio T, James JL, Prescott AR, Tanaka Y, Schaap P: Activated cAMP receptors switch encystation into sporulation. Proc Natl Acad Sci USA. 2009, 106: 7089-7094. 10.1073/pnas.0901617106.PubMedPubMed CentralGoogle Scholar
- Bader S, Kortholt A, Van Haastert PJ: Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP. Biochem J. 2007, 402: 153-161. 10.1042/BJ20061153.PubMedPubMed CentralGoogle Scholar
- Bosgraaf L, Waijer A, Engel R, Visser AJ, Wessels D, Soll D, van Haastert PJ: RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium. J Cell Sci. 2005, 118: 1899-1910. 10.1242/jcs.02317.PubMedGoogle Scholar
- van Egmond WN, Kortholt A, Plak K, Bosgraaf L, Bosgraaf S, Keizer-Gunnink I, van Haastert PJ: Intramolecular activation mechanism of the Dictyostelium LRRK2 homolog Roco protein GbpC. J Biol Chem. 2008, 283: 30412-30420. 10.1074/jbc.M804265200.PubMedPubMed CentralGoogle Scholar
- Kay RR: The biosynthesis of differentiation-inducing factor, a chlorinated signal molecule regulating Dictyostelium development. J Biol Chem. 1998, 273: 2669-2675. 10.1074/jbc.273.5.2669.PubMedGoogle Scholar
- Thompson CR, Kay RR: The role of DIF-1 signaling in Dictyostelium development. Mol Cell. 2000, 6: 1509-1514. 10.1016/S1097-2765(00)00147-7.PubMedGoogle Scholar
- Nayler O, Insall R, Kay RR: Differentiation-inducing-factor dechlorinase, a novel cytosolic dechlorinating enzyme from Dictyostelium discoideum. Eur J Biochem. 1992, 208: 531-536. 10.1111/j.1432-1033.1992.tb17217.x.PubMedGoogle Scholar
- Gilbert OM, Foster KR, Mehdiabadi NJ, Strassmann JE, Queller DC: High relatedness maintains multicellular cooperation in a social amoeba by controlling cheater mutants. Proc Natl Acad Sci USA. 2007, 104: 8913-8917. 10.1073/pnas.0702723104.PubMedPubMed CentralGoogle Scholar
- Yang Z: The power of phylogenetic comparison in revealing protein function. Proc Natl Acad Sci USA. 2005, 102: 3179-3180. 10.1073/pnas.0500371102.PubMedPubMed CentralGoogle Scholar
- Greig D, Travisano M: The prisoner's dilemma and polymorphism in yeast SUC genes. Proc Biol Sci. 2004, 271 (Suppl 3): S25-26. 10.1098/rsbl.2003.0083.PubMedPubMed CentralGoogle Scholar
- Francis D: High frequency recombination during the sexual cycle of Dictyostelium discoideum. Genetics. 1998, 148: 1829-1832.PubMedPubMed CentralGoogle Scholar
- Wallace MA, Raper KB: Genetic exchanges in the macrocysts of Dictyostelium discoideum. J Gen Microbiol. 1979, 113: 327-337.PubMedGoogle Scholar
- Francis D, Eisenberg R: Genetic structure of a natural population of Dictyostelium discoideum, a cellular slime mold. Mol Ecol. 1993, 2: 385-392. 10.1111/j.1365-294X.1993.tb00031.x.PubMedGoogle Scholar
- Muramoto T, Suzuki K, Shimizu H, Kohara Y, Kohriki E, Obara S, Tanaka Y, Urushihara H: Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum. Mech Dev. 2003, 120: 965-975. 10.1016/S0925-4773(03)00136-9.PubMedGoogle Scholar
- Malik SB, Pightling AW, Stefaniak LM, Schurko AM, Logsdon JM: An expanded inventory of conserved meiotic genes provides evidence for sex in Trichomonas vaginalis. PLoS ONE. 2007, 3: e2879-10.1371/journal.pone.0002879.PubMedGoogle Scholar
- Olive EW: Monograph of the Acrasieae. Proc Boston Soc Natur Hist. 1902, 30: 451-513.Google Scholar
- Sussman M: Cultivation and synchronous morphogenesis of Dictyostelium under controlled experimental conditions. Methods Cell Biol. 1987, 28: 9-29. full_text.PubMedGoogle Scholar
- Weber JL, Myers EW: Human whole-genome shotgun sequencing. Genome Res. 1997, 7: 401-409.PubMedGoogle Scholar
- Ewing B, Hillier L, Wendl MC, Green P: Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 1998, 8: 175-185.PubMedGoogle Scholar
- Aparicio S, Chapman J, Stupka E, Putnam N, Chia JM, Dehal P, Christoffels A, Rash S, Hoon S, Smit A, Gelpke MD, Roach J, Oh T, Ho IY, Wong M, Detter C, Verhoef F, Predki P, Tay A, Lucas S, Richardson P, Smith SF, Clark MS, Edwards YJ, Doggett N, Zharkikh A, Tavtigian SV, Pruss D, Barnstead M, Evans C, et al: Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes. Science. 2002, 297: 1301-1310. 10.1126/science.1072104.PubMedGoogle Scholar
- Pfam. [http://pfam.sanger.ac.uk/]
- Ensembl. [http://uswest.ensembl.org/]
- Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, et al: The amphioxus genome and the evolution of the chordate karyotype. Nature. 2008, 453: 1064-1071. 10.1038/nature06967.PubMedGoogle Scholar
- Neurospora crassa Database. [http://www.broadinstitute.org/annotation/genome/neurospora/MultiHome.html]
- TAIR. [http://www.arabidopsis.org/]
- Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Maréchal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R: The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science. 2007, 318: 245-250. 10.1126/science.1143609.PubMedPubMed CentralGoogle Scholar
- Schultz J, Milpetz F, Bork P, Ponting CP: SMART, a simple modular architecture research tool: identification of signaling domains. Proc Natl Acad Sci USA. 1998, 95: 5857-5864. 10.1073/pnas.95.11.5857.PubMedPubMed CentralGoogle Scholar
- Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, Higgins DG, Thompson JD: Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res. 2003, 31: 3497-3500. 10.1093/nar/gkg500.PubMedPubMed CentralGoogle Scholar
- Hall TA: Bioedit: a user-friendly biological sequence alignment editor and analysis program fro Windows 95/98/NT. Nucleic Acids Symp Ser. 1999, 41: 95-98.Google Scholar
- Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19: 1572-1574. 10.1093/bioinformatics/btg180.PubMedGoogle Scholar
- Alba MM, Guigo R: Comparative analysis of amino acid repeats in rodents and humans. Genome Res. 2004, 14: 549-554. 10.1101/gr.1925704.PubMedPubMed CentralGoogle Scholar
- Lowe TM, Eddy SR: tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997, 25: 955-964. 10.1093/nar/25.5.955.PubMedPubMed CentralGoogle Scholar
- Hinas A, Reimegard J, Wagner EG, Nellen W, Ambros VR, Soderbom F: The small RNA repertoire of Dictyostelium discoideum and its regulation by components of the RNAi pathway. Nucleic Acids Res. 2007, 35: 6714-6726. 10.1093/nar/gkm707.PubMedPubMed CentralGoogle Scholar
- Protein coding regions of the D. purpureum genome. [ftp://ftp.jgi-psf.org/pub/JGI_data/Dictyostelium_purpureum/v1.0/annotation/Dicpu1_best_genes.gff.gz]
- Protein coding regions of the D. disdoideum genome. [http://dictybase.org/download/gff3/dicty_gff3_10212009.zip]
- LocARNA. [http://rna.tbi.univie.ac.at/cgi-bin/LocARNA.cgi]
- RNALogo: CreateLogo. [http://rnalogo.mbc.nctu.edu.tw/createlogo.html]
- Remm M, Storm CE, Sonnhammer EL: Automatic clustering of orthologs and in-paralogs from pairwise species comparisons. J Mol Biol. 2001, 314: 1041-1052. 10.1006/jmbi.2000.5197.PubMedGoogle Scholar
- Finn RD, Tate J, Mistry J, Coggill PC, Sammut SJ, Hotz HR, Ceric G, Forslund K, Eddy SR, Sonnhammer EL, Bateman A: The Pfam protein families database. Nucleic Acids Res. 2008, D281-288. 36 Database
- Edgar RC: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004, 32: 1792-1797. 10.1093/nar/gkh340.PubMedPubMed CentralGoogle Scholar
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.PubMedGoogle Scholar
- Polyketide synthase gene analyses. [http://www.mrc-lmb.cam.ac.uk/genomes/madanm/rrk]
- Bockaert J, Pin JP: Molecular tinkering of G protein-coupled receptors: an evolutionary success. EMBO J. 1999, 18: 1723-1729. 10.1093/emboj/18.7.1723.PubMedPubMed CentralGoogle Scholar
- Prabhu Y, Eichinger L: The Dictyostelium repertoire of seven transmembrane domain receptors. Eur J Cell Biol. 2006, 85: 937-946. 10.1016/j.ejcb.2006.04.003.PubMedGoogle Scholar
- Pandey S, Nelson DC, Assmann SM: Two novel GPCR-type G proteins are abscisic acid receptors in Arabidopsis. Cell. 2009, 136: 136-148. 10.1016/j.cell.2008.12.026.PubMedGoogle Scholar
- King N, Hittinger CT, Carroll SB: Evolution of key cell signaling and adhesion protein families predates animal origins. Science. 2003, 301: 361-363. 10.1126/science.1083853.PubMedGoogle Scholar
- Joseph JM, Fey P, Ramalingam N, Liu XI, Rohlfs M, Noegel AA, Muller-Taubenberger A, Glockner G, Schleicher M: The actinome of Dictyostelium discoideum in comparison to actins and actin-related proteins from other organisms. PLoS ONE. 2008, 3: e2654-10.1371/journal.pone.0002654.PubMedPubMed CentralGoogle Scholar
- Torija MJ, Novo M, Lemassu A, Wilson W, Roach PJ, François J, Parrou JL: Glycogen synthesis in the absence of glycogenin in the yeast Saccharomyces cerevisiae. FEBS Lett. 2005, 579: 3999-4004. 10.1016/j.febslet.2005.06.007.PubMedGoogle Scholar
- Deschamps P, Colleoni C, Nakamura Y, Suzuki E, Putaux JL, Buléon A, Haebel S, Ritte G, Steup M, Falcón LI, Moreira D, Löffelhardt W, Raj JN, Plancke C, d'Hulst C, Dauvillée D, Ball S: Metabolic symbiosis and the birth of the plant kingdom. Mol Biol Evol. 2008, 25: 536-548. 10.1093/molbev/msm280.PubMedGoogle Scholar
- Elbein AD, Pan YT, Pastuszak I, Carroll D: New insights on trehalose: a multifunctional molecule. Glycobiology. 2003, 13: 17R-27R. 10.1093/glycob/cwg047.PubMedGoogle Scholar
- Blanton RL, Fuller D, Iranfar N, Grimson MJ, Loomis WF: The cellulose synthase gene of Dictyostelium. Proc Natl Acad Sci USA. 2000, 97: 2391-2396. 10.1073/pnas.040565697.PubMedPubMed CentralGoogle Scholar
- Wang YZ, Slade MB, Gooley AA, Atwell BJ, Williams KL: Cellulose-binding modules from extracellular matrix proteins of Dictyostelium discoideum stalk and sheath. Eur J Biochem. 2001, 268: 4334-4345. 10.1046/j.1432-1327.2001.02354.x.PubMedGoogle Scholar
- West CM, Nguyen P, van der Wel H, Metcalf T, Sweeney KR, Blader IJ, Erdos GW: Dependence of stress resistance on a spore coat heteropolysaccharide in Dictyostelium. Eukaryot Cell. 2009, 8: 27-36. 10.1128/EC.00398-07.PubMedPubMed CentralGoogle Scholar
- West CM: Comparative analysis of spore coat formation, structure, and function in Dictyostelium. Int Rev Cytol. 2003, 222: 237-293. full_text.PubMedGoogle Scholar
- Yu YK, Wootton JC, Altschul SF: The compositional adjustment of amino acid substitution matrices. Proc Natl Acad Sci USA. 2003, 100: 15688-15693. 10.1073/pnas.2533904100.PubMedPubMed CentralGoogle Scholar
- Huang X, Brutlag DL: Dynamic use of multiple parameter sets in sequence alignment. Nucleic Acids Res. 2007, 35: 678-686. 10.1093/nar/gkl1063.PubMedPubMed CentralGoogle Scholar
- Yang Z: PAML 4: phylogenetic analysis by maximum likelihood. Mol Biol Evol. 2007, 24: 1586-1591. 10.1093/molbev/msm088.PubMedGoogle Scholar
- Lopez-Bigas N, Ouzounis CA: Genome-wide identification of genes likely to be involved in human genetic disease. Nucleic Acids Res. 2004, 32: 3108-3114. 10.1093/nar/gkh605.PubMedPubMed CentralGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.