The dissection of transcriptional modules regulated by various drugs of abuse in the mouse striatum
- Marcin Piechota†1,
- Michal Korostynski†1,
- Wojciech Solecki1,
- Agnieszka Gieryk1,
- Michal Slezak1,
- Wiktor Bilecki1,
- Barbara Ziolkowska1,
- Elzbieta Kostrzewa1,
- Iwona Cymerman2,
- Lukasz Swiech2,
- Jacek Jaworski2 and
- Ryszard Przewlocki1Email author
© Piechota et al.; licensee BioMed Central Ltd. 2010
Received: 9 February 2010
Accepted: 4 May 2010
Published: 4 May 2010
Various drugs of abuse activate intracellular pathways in the brain reward system. These pathways regulate the expression of genes that are essential to the development of addiction. To reveal genes common and distinct for different classes of drugs of abuse, we compared the effects of nicotine, ethanol, cocaine, morphine, heroin and methamphetamine on gene expression profiles in the mouse striatum.
We applied whole-genome microarray profiling to evaluate detailed time-courses (1, 2, 4 and 8 hours) of transcriptome alterations following acute drug administration in mice. We identified 42 drug-responsive genes that were segregated into two main transcriptional modules. The first module consisted of activity-dependent transcripts (including Fos and Npas4), which are induced by psychostimulants and opioids. The second group of genes (including Fkbp5 and S3-12), which are controlled, in part, by the release of steroid hormones, was strongly activated by ethanol and opioids. Using pharmacological tools, we were able to inhibit the induction of particular modules of drug-related genomic profiles. We selected a subset of genes for validation by in situ hybridization and quantitative PCR. We also showed that knockdown of the drug-responsive genes Sgk1 and Tsc22d3 resulted in alterations to dendritic spines in mice, possibly reflecting an altered potential for plastic changes.
Our study identified modules of drug-induced genes that share functional relationships. These genes may play a critical role in the early stages of addiction.
Drug addiction is a brain disease with prominent hazardous effects, including the collapse of health and social and economic status . Acute exposure to drugs of abuse initiates molecular and cellular alterations in the central nervous system [2, 3] that lead to an increased overall vulnerability to addiction with subsequent drug exposures . These drug-induced alterations employ changes in gene transcription that result in the synthesis of new proteins . Therefore, one of the important goals of addiction research is to identify the drug-induced gene expression changes in the specific brain structures that are related to the addictive properties of various drugs.
The major neural target sites of addictive drugs are the ventral and the dorsal striatum, that is, the brain regions that control reward sensitivity, motor function and habit learning . The dorsal striatum is thought to underlie stimulus-response and spatial learning, and the ventral striatum is involved in appetitive behavior and reinforcement [7, 8]. However, to some extent, these functions might overlap [9, 10]. All addictive drugs elevate dopamine levels in the striatum, and this effect is associated with reinforcing drug properties . However, the pharmacological mechanisms and neural substrates involved in mediating the rewarding action are different for various drugs. Psychostimulants directly influence extracellular dopamine levels in the striatum through inhibitory effects on dopamine reuptake [12, 13]. Opiates inhibit GABAergic inhibitory neurons in the ventral tegmental area and activate dopaminergic neurons projecting to the striatum . In addition, opiates directly bind to opioid receptors located on striatal interneurons . Ethanol acts on GABAergic interneurons in the ventral tegmental area that, in turn, modulate the activity of dopaminergic neurons and the action of neurotransmitter-gated ion channels . Nicotine enhances reward-related dopamine release by activating nicotinic acetylcholine receptors [17, 18]. Therefore, it is believed that the combination of dopamine-dependent neurotransmission and endogenous opioid-dependent modulation is responsible for the acquisition of drug addiction [4, 19]. The molecular and genomic mechanisms by which drugs of abuse induce neuroplastic changes related to addiction remain largely unknown .
Several studies have evaluated changes in gene expression profiles in the brain after administration of drugs of abuse (reviewed in ). Exposure to psychostimulants induces the activity-dependent gene expression of several transcription activators and repressors [22, 23]. Opioids and ethanol regulate the transcription of genes involved in metabolic functions and a group of genes encoding heat-shock proteins [24–28].
Genomic research strategies have recently transitioned from the search for unknown genes to the identification and evaluation of coordinated gene networks and transcriptional signatures . New opportunities arising from the analysis of these networks include identifying novel relationships between genes and signaling pathways, connecting biological processes with the regulation of gene transcription, and associating genes and gene expression with diseases [30, 31]. The identification of gene networks requires large gene expression data sets with multiple data points . The transcriptional response to a drug treatment analyzed during a time-course suits the above strategy perfectly.
Exploring dynamic changes in brain gene expression profiles is possible only in animal models. In these models, assessments of the behavioral effects of drugs of abuse are well established. Therefore, integrating brain gene transcription and phenotypic information provides us with a unique opportunity to associate the addictive potential of the drugs with the molecular responses activated by these drugs [33, 34]. The limitations of such a strategy include differences in drug responses between humans and rodents and the extreme complexity of the analyzed tissue. Despite these limitations, the obtained results may provide new insights into the molecular control of drug addiction.
In this study, we aimed to identify the transcriptional networks activated by different classes of addictive drugs and to translate the gene expression patterns into biological themes that are related to the development of addiction.
Comparison of rewarding and stimulant drug properties
An independent group of animals was tested for drug-induced motor behavior. Cocaine, methamphetamine, heroin and morphine treatment significantly increased locomotor activity following acute drug administration (repeated-measures ANOVA, Newman-Keuls test, P < 0.05; Figure 1b). Ethanol and nicotine treatment did not produce locomotor activation in comparison to saline-treated controls. The behavioral data were further used to analyze associations between phenotypes and transcriptome alterations.
Whole-genome gene expression profiling
We applied a strategy of detailed time-course studies of gene expression alterations following acute administration of various drugs of abuse using Illumina Whole-Genome 6 microarrays. To analyze the dynamics of early, intermediate and relatively late changes in mRNA abundance, the analysis was performed at four time points (1, 2, 4 and 8 h following drug injection).
Functional classes, results from literature, and transcription factor binding sites associated with drug-regulated patterns of gene expression
Protein phosphatase activity
Rodriguez et al. , cocaine 1 h
Jayanthi et al. , methampethamine 2 h
Lemberger et al. , kainic acid 1 h
Protein dimerization activity
Ryan et al. , domoic acid 0.5 and 1 h
Regulation of transcription
Impey et al. , forskolin 1 h
Small GTPase mediated signal transduction
Sanchis-Segura et al. , morphine 4 h
Treadwell et al. , ethanol 6 h
Sato et al. , dexamethasone 2 h
Intracellular signaling cascade
Lemberger et al. , kainic acid 1 h
Ryan et al. , domoic acid 4 h
Enzyme inhibitor activity
Korostynski et al. , morphine 4 h
Ryan et al. , domoic acid 4 h
Response to stress
McClung et al. , morphine withdrawal
Treadwell et al. , ethanol 6 h
Chen et al. , heart failure left ventricular assist device (LVAD)
Regulation of developmental process
Korostynski et al. , morphine 4 h
Magnesium ion binding
McClung et al. , morphine withdrawal
Anatomical structure morphogenesis
Ryan et al. , domoic acid 4 h
Calcium ion binding
Treadwell et al. , ethanol 6 h
Transmembrane transporter activity
Hasan et al. , chronic oxycodone
Furthermore, we estimated the false discovery rate (percent FDR) to answer the question of how large was the fraction of drug-responsive genes discovered at the assumed threshold . The maximum number of true positive genes altered in the striatum by drugs of abuse (drug factor, 104 transcripts) was found at a 29% FDR. Beyond that level, the number of true positives did not increase. Surprisingly, the number of true positives remained stable (84 to 104 transcripts, mean = 94.4 ± 4.9) over a wide range of FDR (4.7 to 56.3%). The results for the drug factor are in contrast to alterations in the striatal gene expression profile related to the time point of the experiment (time factor). The maximum number of true positive genes (5,442 transcripts) for the time factor was found at a 69.8% FDR and increased linearly in the range 0.1 to 69.8% FDR (Additional file 1).
The above observations suggest a rather unexpected conclusion. While the diurnal cycle alters a vast fraction of the brain transcriptome, drugs regulated the expression of a limited number of genes (approximately 100), and this alteration was robust. The number of genes obtained using Bonferroni correction (42 transcripts) was equal to the number of genes obtained at a 0.1% FDR threshold. Therefore, at the chosen threshold, we identified 40.3% (42 of 104 transcripts) of genes altered by drugs of abuse with 99.9% confidence. The complete results of the ANOVA, including two different methods of correction for multiple comparisons (Bonferroni correction and percent FDR) for both time and drug factors are provided in Additional file 2.
Identification of drug-regulated gene expression patterns
Hierarchical clustering revealed two major drug-responsive gene transcription patterns (arbitrarily described as A and B; Figure 2a). Pattern B consisted of three subsequent subclusters (arbitrarily described as B1, B2 and B3). Example genes from the particular patterns include: pattern A, Fos, Egr2 and Homer1; pattern B1, Sgk1, Plekhf1 and Rasd1; pattern B2, Tsc22d3, Cdkn1a and Map3k6; and pattern B3, Fkbp5, S3-12 and Sult1a1.
To search for other drug-responsive transcriptional networks, we performed additional hierarchical clustering on the lists of genes obtained with 5% (a threshold commonly used in the literature) and 29% (the maximum number of true positive results) FDRs. With these less restrictive statistical criteria, we found no other networks with distinct gene expression profiles (Additional file 4). Assuming that we identified all the major gene patterns altered by drugs of abuse and taking into consideration the fact that Bonferroni correction for multiple testing is very conservative, lists of genes with a significant level of Pearson's correlation to the main clusters (P < 1 × 10-10; FDR for this analysis was estimated at <0.1%) were extracted and analyzed.
Gene Ontology (GO) enrichment, literature mining and identification of cis-regulatory elements was performed on extended lists of transcripts from each gene expression pattern: A, 38 genes; B1, 45 genes; B2, 31 genes; and B3, 18 genes. Due to the similar profiles of the B1 and B2, as well as the B2 and B3, gene subsets, the lists partially overlapped by 27% and 45%, respectively (Additional file 2). One gene (Car12) was excluded from the analysis due to its outlying gene expression profile.
The drug-responsive genes are randomly distributed throughout the entire mouse genome. Chromosome localizations are shown in Additional file 5.
Comparison of drug effects on the striatal transcriptome
The results of gene expression profiling revealed differences and similarities in the transcriptional responses to the various drugs (Figure 2; Additional file 6). Pattern A was induced 1 to 2 h after injection of cocaine or methamphetamine and 4 h after injection of morphine or heroin. Pattern B consisted of three subsequent subclusters: B1, induced 1 to 2 h after injection of ethanol, morphine, heroin, methamphetamine and cocaine; B2, induced 2 to 4 h after injection of ethanol, morphine, heroin and methamphetamine; and B3, induced 4 h after injection of ethanol, morphine and heroin. The only pattern common to all inspected drugs was pattern B1. However, this pattern was induced by different drugs to different degrees.
The drugs were divided into two groups. One, including cocaine and methamphetamine, exhibited high and early induction of pattern A and low or absent induction of pattern B. The second group, including ethanol, morphine and heroin, elicited high induction of pattern B. The complete results of a Tukey's post-hoc test (P < 0.05, drug versus saline) after ANOVA are provided in Additional file 2.
Functional classification of drug-responsive genes
To identify functional associations between the genes with expression induced by drugs, we used three different data-mining tools (Figure 2b, Table 1). To characterize the transcriptional representation of biological processes, a list of genes from each gene expression pattern was analyzed by GO. Among the most abundant group of genes in pattern A, functional clusters of transcripts connected with protein phosphatase activity (32.4-fold enrichment, P < 0.01; for example, Dusp1, Dusp6), rhythmic processes (14.7-fold, P < 0.05; for example, Per1, Per2) and transcriptional regulatory activity (3-fold, P < 0.001; for example, Fos, Egr2) were over-represented.
The group of genes from pattern B1 was enriched in transcripts involved in small GTPase-mediated signal transduction (5.9-fold, P < 0.01; for example, Rhou, Rasd1), apoptosis (5-fold, P < 0.01; for example, Gadd45 g, Sgk1) and the cell cycle (4.7-fold, P < 0.01; for example, Gadd45 g, Nedd9). Analysis of pattern B2 revealed the enrichment of genes connected to enzyme inhibitor activity (8.9-fold, P < 0.05; for example, Cdkn1a, Angptl4), the stress response (4.2-fold, P < 0.01; for example, Cdkn1a, Tsc22d3) and regulation of cell differentiation (2.5-fold, P < 0.05; for example, Plekhf1, Zbtb16). Over-representation of transcripts involved in magnesium ion binding (8.5-fold, P < 0.05; for example, Itgad, Atp10a) was observed within gene expression pattern B3. A detailed description of the results of GO classification is included in Additional file 7.
Comparison with previously reported gene expression profiles
To find points of reference for our results, we compared the lists of genes from the co-expressed gene patterns with previously described changes in gene expression profiles. Literature mining was based on the lists of genes reported as regulated in published manuscripts or found in publicly available datasets. Overall, we compared our data with 1,267 gene sets (Additional file 8).
We found high similarity with pattern A to lists of genes regulated following cocaine (P = 1.33 × 10-36) and methamphetamine (P = 1.04 × 10-13, FDR-corrected) administration . Moreover, significant enrichment of genes regulated by kainic acid (P = 5.88 × 10-13) and domoic acid (P = 3.52 × 10-12) in the brain and by forskolin (P = 3.87 × 10-12) in vitro was also found in this group [41–44]. All of these in vivo effects were observed at a relatively early time point (1 to 2 h after injection) and were connected with the induction of a group of immediate early gene (IEG) transcription factors and neuroplasticity-related genes like Fos, Arc, Npas4 and Homer1.
Drug-induced transcription pattern B revealed different links with the published gene expression profiles than gene pattern A. The effects of morphine (P = 1.15 × 10-29) and ethanol (P = 1.87 × 10-11) on the activation of gene expression pattern B were in agreement with previous results [45, 46]. These genes were induced between 2 and 4 h following drug injection. The regulation of gene expression pattern B was somewhat similar to the effects of the glucocorticoid receptor (GR) agonist dexamethasone in the hypothalamus (P = 1.57 × 10-8) . Moreover, expression pattern B contained genes reported to be up-regulated in response to domoic acid (P = 1.41 × 10-18) at a relatively late time point (4 h after injection; Table 1) .
We also found that gene expression pattern A was similar to the group of dopamine receptor 1 (D1R) antagonist (SCH23390)-sensitive methamphetamine-responsive genes (P = 1.05 × 10-6). In contrast, pattern B1 was similar to the group of SCH23390-resistant methamphetamine-responsive genes (P = 4.20 × 10-5) . A detailed description of the results of literature mining is included in Additional file 8.
Identification of transcription factor binding sites
To discover molecular factors that are involved in the transcriptional control of the discovered gene expression patterns, we used an in silico method of transcription factor binding site (TFBS) identification. We analyzed gene promoters based on the assumption that a subset of the co-expressed genes may be co-regulated by common transcription factors. For this purpose, we developed a new tool for the discovery of over-represented TFBSs: the cREMaG database (see Materials and methods).
Gene expression pattern A revealed the highest over-representation of binding sites for serum response factor (SRF)/serum-responsive elements (5.7-fold higher than expected by chance, P = 9.5 × 10-3). Significant over-representation of TFBSs for transcription factor cyclic AMP-response element binding protein (CREB)/cyclic AMP response elements (3.9-fold, P = 6.8 × 10-3) was also found. We identified an over-representation of cyclic AMP response elements (the binding site for the CREB transcription factor) and serum-responsive elements (the binding site for the SRF transcription factor) in the core promoter regions of genes with expression pattern A. The complementary roles of these transcription factors have been independently confirmed [41, 48].
The analysis of the promoter regions of pattern B1 genes indicated an over-representation of the binding site for transcription factors of the FOX family, Foxd3/FRE (4.4-fold, P = 1.96 × 10-2). Forkhead transcription factors are implicated in the neuronal response to oxidative stress . Promoter regions of genes from expression pattern B2 contained relatively more binding sites for steroid hormones NR1H2-RAXR (3.5-fold, P = 2.88 × 10-2) and Ar/ARE (3.3-fold, P = 7.4 × 10-2) with transcriptional activity. An enrichment of binding sites for nuclear receptors in promoter regions, including the androgen receptor (ARE/Ar), was found, suggesting that genes from this subgroup may be regulated by steroid hormones. Over-representation of binding sites for Fos/AP1 (6.7-fold, P = 1.03 × 10-2) and NR3C1/GRE (5.6-fold, P = 5.8 × 10-3) was observed within promoter regions of genes from pattern B3 (Table 1). Components of the transcriptional complex AP-1 (Fos, Fosb) exhibited gene expression pattern A. Therefore, the occurrence of an AP-1 site in the promoter regions of genes expressed relatively late following drug administration may indicate target genes for the drug-activated transcription factors. The second putative mechanism of B3 gene regulation is related to the effects of glucocorticoid hormones on the central nervous system.
Pharmacological dissection of drug-regulated gene patterns
The selected regulatory processes were tested for their influence on drug-induced gene expression pattern A. Pre-treatment with a D1R antagonist (SCH23390) blocked drug-induced CREB1/SRF-mediated gene transcription in the striatum, with a 126% reduction (26% of induction below basal level) in the level of cocaine activation (P < 0.001) (Figure 4a). This observation suggested that the regulatory intracellular cascades are activated mainly in striatal cells containing D1R. At the same time, SL327, an inhibitor of extracellular signal-regulated kinase (ERK1/2) activator kinase MEK1/2, inhibited the cocaine-activated expression of genes from pattern A, with a 107% reduction (7% of induction below basal level) in the level of cocaine activation (P < 0.001) (Figure 4b). This observation clearly indicates the involvement of the ERK1/2 signaling pathway .
Moreover, the administration of the histone deacetylase inhibitor trichostatin before cocaine administration provoked an intensification of the transcriptional response, with a 33% increase in the level of cocaine activation (Figure 4c). This observation suggested that the induction of genes from the expression pattern A may require enhanced chromatin unfolding .
Opioids increased the abundance of mRNAs from expression pattern A 4 h after injection. Pretreatment with the protein synthesis inhibitor cycloheximide (CHX) inhibited this induction, with a 66% reduction in the level of heroin activation (P < 0.05) (Figure 4d), which indicates that this relatively late response depends on protein translation.
All of the compared drugs induced gene transcription pattern B1 in the striatum. The relatively early transcriptional response to heroin (CHX blocked 87% of activation, P < 0.001) and ethanol (CHX blocked 72% of activation, P < 0.05) was blocked by an inhibitor of protein synthesis (Figure 4d, e). Similar effects of CHX were observed on the induction of gene expression pattern B2 in response to heroin (CHX blocked 106% of activation, 6% of activation below basal level, P < 0.001) and ethanol (CHX blocked 104% of activation, P < 0.001) (Figure 4d, e).
Microarray results indicated the inhibitory effects of corticotropin-releasing factor (CRF) receptor 1 (CRFR1) and GR antagonists (antalarmin and RU486, respectively, blocked 65% of ethanol activation, P < 0.05) on gene expression activation of the B3 subcluster (Figure 4f, g). RU486 also altered the expression of several B1 genes, for example, Sgk1 and Plekhf1 (Figure 4g). Therefore, the influence of GR receptor blockage on ethanol-induced expression of B1genes could not be correctly evaluated.
Correlation with behavioral drug effects
To link the gene expression patterns with drug-related phenotypes, we analyzed the correlations between the transcriptional and behavioral drug effects in mice. Mutual interactions between the brain gene expression and behavioral profiles are complex and multidimensional. Therefore, it is difficult to define them using analyses performed with only the few available data points. However, even speculative results obtained from this analysis create the unique possibility of assigning different transcriptional alterations induced by various drugs to drug-related phenotypes. We observed a positive correlation of r = 0.62 (Pearson's method, P < 0.001) between the level of drug-induced locomotor activation and the degree of transcriptional response of gene expression pattern A. Additionally, we found a significant correlation between the acute induction of B1 genes and the rewarding effect of the drug (r = 0.7, Pearson's method, P < 0.05; Figure 1c, d; Additional file 9).
Evaluation of two drug-regulated genes at the mRNA and protein levels
We selected two genes from expression pattern B for further evaluation. The first gene, Sgk1, encodes the SGK protein (serum-and glucocorticoid-inducible kinase) and exhibited the B1 pattern. The second gene, Tsc22d3, encodes the GILZ protein (glucocorticoid-induced leucine-zipper protein) and exhibited the B2 pattern. We inspected alterations in mRNA abundance in the striatum during the acquisition and expression of the morphine-induced CPP. Both genes were induced 4 h after each of three subsequent sessions of morphine-induced (20 mg/kg i.p.) conditioning (between 1.5-fold and 3-fold over the control group). However, transcription of Sgk1 and Tsc22d3 was not activated during the behavioral expression of morphine-induced CPP (Figure 3b).
Western blotting was used to determine whether the changes in gene expression were translated into alterations in protein levels. The morphine-induced increase in Sgk1 abundance was associated with a significant decrease in the level of the protein (0.75-fold). Therefore, Sgk1 expression changes might be a compensatory effect to the loss of the protein. Up-regulation of Tsc22d3 was associated with an increase in the corresponding protein level (approximately 1.5-fold; Figure 5c). Double-immunofluorescence labeling with neuronal (NeuN) and astroglial (S100B) markers was used to identify cells that expressed SGK (Sgk1) and GILZ (Tsc22d3) proteins. In the mouse striatum, both genes appeared to be expressed mainly in neurons (Figure 5b).
Drug-responsive genes are involved in the formation of dendritic spines
In this study, we aimed to define the sequence of molecular changes in the striatum in response to various drugs of abuse. We estimated that the number of genes induced by administration of the drugs of abuse was limited to approximately 100. Other studies reveal various numbers of genes with altered expression by drugs of abuse [26, 41, 44, 46]. However, their estimations are based on arbitrary significance or fold-change thresholds, whereas our prediction was based on the number of true positives through a wide range of false discovery rates. The transcriptome changes produced by drugs of abuse were in contrast to alterations related to the diurnal cycle. While drugs of abuse produced few robust changes, the diurnal cycle alters the levels of several thousand transcripts (Additional file 1).
We found that almost all identified genes were regulated in concert with other genes in the form of two drug-responsive transcriptional modules. No other transcriptional modules were identified, even at lower significance thresholds. Therefore, we can assume that this study depicted all of the main patterns of induction in the mouse striatum after administration of drugs of abuse in rewarding doses. However, a recent study showed that after administration of higher, neurotoxic doses of methamphetamine, distinct gene expression patterns appear .
The first identified pattern (pattern A) consisted of IEGs, which are well described and commonly used as markers of neuronal activation [57–59]. Particular genes from this cluster were previously identified in the response to drugs of abuse, including Fos, Fosb and Egr1 [60, 61]. Using bioinformatic analyses, we were able to postulate a role of the CREB and SRF transcription factors as main regulators of IEGs, which has been suggested previously [62, 63]. Previous studies indicate that the cocaine-induced activity of CREB and SRF in the striatum is dependent on the D1R-downstream MEK/ERK signaling pathway [50, 64]. Using pharmacological intervention, we established the role of D1R and the MEK/ERK signaling pathway for the whole group of IEGs. However, the role of a group of simultaneously expressed IEGs in neurons is not known. Some genes from pattern A (Npas4, Homer1a and Arc) may be involved in protecting against neuronal overexcitability [65, 66]. Npas4 regulates inhibitory synapse development in an activity-dependent manner and diminishes the excitatory synaptic input neurons receive . Homer1a appears to participate in the attenuation of the gradual inhibition of glutamate receptor-dependent calcium mobilization, as well as in mitogen-activated protein kinase activation . Finally, increased expression of Arc may play a role in reducing AMPA receptor-mediated synaptic transmission [69, 70]. We clearly demonstrated a lack of an effect of ethanol on the induction of IEGs in the striatum. It is worth mentioning that the induction of Fos (a member of pattern A) by cocaine is inhibited by ethanol [71–73]. We demonstrated that this lack of induction by ethanol was true for all IEGs. Therefore, the role of striatal IEGs in the development of ethanol addiction is rendered questionable.
The second identified pattern (pattern B) is relatively unknown. To our knowledge, this is the first comprehensive report describing the time- and drug-dependent induction of this gene expression pattern, although some of these genes have been previously reported by us and others [26, 45, 46]. This pattern consists of three subsequent sub-clusters (B1, B2 and B3). The examined drugs of abuse, with diverse pharmacological actions and behavioral effects, were all able to induce gene transcription of the relatively early pattern B1. Expression pattern B1 appeared to depend on several regulatory proteins, for example, transcription factors of the FOX family. The data also imply that patterns B2 and B3 appear to be regulated by steroid hormones that respond to morphine, heroin and ethanol. This is in agreement with activation of the hypothalamic-pituitary-adrenal axis (HPA) after administration of opioids and ethanol [74, 75]. Our pharmacological dissection of drug-regulated gene expression profiles showed the inhibitory effects of CRFR1 and GR antagonists on ethanol-activated gene expression of the B2 and B3 subclusters. Therefore, the present results suggested that ethanol- and/or opioid-induced activation of these genes depends on HPA and the release of steroid hormones from the adrenal gland. This CRF- and GR-dependent signaling system is emerging as a key element of the neuroadaptive changes that are induced by drugs of abuse [76–78]. The identification of novel drug-responsive genes downstream of CRF/GR may help uncover the molecular mechanisms linking stress and addiction [79, 80]. Pattern B genes modulate various aspects of cell functioning: hexose transport (Slc2a1), lipid metabolism (Angptl4, Pparg), regulation of sodium channels and the actin cytoskeleton (Sgk1) and regulation of the cell cycle (Cdkn1a), to mention just a few [81–84]. Our results clearly revealed qualitative and quantitative differences between the transcriptional networks affected by drugs of abuse. Therefore, it appears that various molecular mechanisms induced by drugs may lead to common addictive behaviors.
Previous studies show that cocaine administration leads to the release of glucocorticoids . However, in our experiments, cocaine did not induce glucocorticoid-responsive genes (patterns B2 and B3). Expression of these genes was also affected relatively weakly by methamphetamine, compared to the much stronger effects of opioids and ethanol. Notably, both psychostimulants were the only drugs in our study that produced a prominent induction of IEGs (pattern A) within the first hour after drug injection. The pattern A genes included components of the transcription factor AP-1 (Fos, Fosl2, Fosb). Since AP-1 is a potent repressor of GR activity , it seems that GR-mediated effects of HPA activation on gene transcription in the striatum after psychostimulant administration may have been inhibited by previous induction of the AP-1 complex proteins due to D1R activation. Moreover, the identified clusters A and B contained their self-repressors. The IEG group contained ICER, an isoform of Crem, which acts as a powerful repressor of cyclic AMP-induced transcription . Pattern B, which was partially controlled by glucocorticoids, includes Fkbp5, a chaperone that inhibits GR translocation into the nucleus .
HPA activation is one of the most recognized attributes of stress. Moreover, HPA activation is also a common effect of various drugs of abuse [74, 75, 85, 89]. Chronic exposure to stress is associated with increased vulnerability to addiction [90, 91]. The enhancing effects of stress on drug self-administration have also been documented in animal models . Moreover, Mantsch et al.  show that corticosterone itself produces almost the same effects on drug taking as stress. Therefore, it is possible that corticosterone released after drug administration enhances the rewarding properties of the subsequent drug doses in the same way stress does. The mechanism of corticosterone contribution to addiction vulnerability is not well understood. Steroid-mediated enhancement of mesocorticolimbic dopamine neuron activity has been suggested to play a role .
A correlation analysis between the induction of gene clusters and the behavioral effects of particular drugs revealed that the induction of both B1 and B2 gene patterns may be associated with the rewarding effects of drugs. The present results suggested that two genes, representatives of patterns B1 (Sgk1) and B2 (Tsc22d3), might be associated with neuroplastic changes after administration of drugs of abuse. Some other authors have attempted to identify the role of Sgk1 in the central nervous system [94–96]. We demonstrated that knockdown of Sgk1 expression in neurons caused lower protrusion density and altered formation of dendritic spines . GILZ (Tsc22d3) has already been considered in the context of its neuronal function [42, 45, 80]. However, we are the first to show that knockdown of Tsc22d3 provoked changes in spine morphology. Rather than mushroom-shaped spines, GILZsh-transfected neurons had thin, long, filopodia-like protrusions. These cellular changes may reflect an altered potential for neuronal plasticity and could be involved in the positive effect of corticosterone on vulnerability to addiction.
We have identified two gene expression patterns that were induced in the striatum by various drugs of abuse and demonstrate that these patterns are the only ones induced by rewarding doses of these drugs. The gene patterns were not equally induced by the various drug classes. Therefore, clear differences between various drugs of abuse exist. We then identified upstream factors that control the discovered patterns. One of the patterns is at least partially controlled by HPA activation. We proposed a molecular mechanism that is involved in the HPA-activated enhancement of drugs' rewarding properties. Finally, we selected two genes and confirmed their influence on neuronal plasticity. In conclusion, this study provides valuable comparisons of the actions of various drugs of abuse on the striatal transcriptome and identifies potential target genes responsible for drug-induced neuroplasticity.
Materials and methods
Adult male (8 to 10 weeks old) C57BL/6J inbred mice were housed 6 to 10 per cage, under a 12-h dark/light cycle, with free access to food and water. Animals weighing 20 to 30 g were used throughout the experiments. The animal protocols used in the study were approved by the local Bioethics Commission at the Institute of Pharmacology, Polish Academy of Sciences (Krakow, Poland).
Mice were sacrificed by decapitation 1, 2, 4 or 8 h after a single morphine (20 mg/kg), heroin (10 mg/kg), ethanol (2 g/kg), nicotine (1 mg/kg), methamphetamine (2 mg/kg) or cocaine (25 mg/kg) i.p. injection, with respective saline and naïve control groups. The inhibitors and antagonists used in the secondary microarray experiment were i.p. injected 30 minutes before any of the drugs of abuse and were dissolved in an appropriate vehicle: 1 mg/kg SCH23390 (Biotrend, Koln, Germany) in saline; 30 mg/kg SL327 (Biotrend) in dimethyl sulfoxide (DMSO; Sigma-Aldrich, Steinheim, Germany); 40 mg/kg RU486 (Biotrend) in 3% Tween 20 (Sigma-Aldrich); 75 mg/kg cycloheximide (Biotrend) in saline; and 20 mg/kg antalarmin (Sigma-Aldrich) in 10% Cremophor EL (Sigma-Aldrich). Trichostatin (Sigma-Aldrich; 1 mg/kg in DMSO) injections were given 2 h before the cocaine. The doses of inhibitors/antagonists were based on the literature, paying particular attention to their ability to block drug-induced behavior.
CPP tests were performed using an unbiased procedure in a three-arm apparatus. The experiment consisted of the following phases separated by 24 h: pre-conditioning test (day 0), conditioning with a drug dose as explained above (days 1, 3, 5), conditioning with saline (days 2, 4, 6) and post-conditioning test (day 7). For ethanol and nicotine, the procedure was prolonged to five sessions of conditioning. An independent group of animals was tested for drug-induced motor activation. Locomotor activity was measured in an activity cage in 15-minute intervals for 4 h following acute drug treatment.
Tissue collection and RNA preparation
Samples containing the rostral part of the caudate putamen and the nucleus accumbens (referred to hereafter as the striatum) were collected. Tissue samples were placed in RNAlater reagent (Qiagen Inc., Valencia, CA, USA) and preserved at -70°C. Samples were thawed at room temperature and homogenized in 1 ml Trizol reagent (Invitrogen, Carlsbad, CA, USA). RNA was isolated following the manufacturer's protocol and further purified using the RNeasy Mini Kit (Qiagen Inc.). The total RNA concentration was measured using a NanoDrop ND-1000 Spectrometer (NanoDrop Technologies Inc., Montchanin, DE, USA). RNA quality was determined by chip-based capillary electrophoresis using an RNA 6000 Nano LabChip Kit and Agilent Bioanalyzer 2100 (Agilent, Palo Alto, CA, USA), according to the manufacturer's instructions. RNA from two mice was pooled to create a sample for each microarray.
Gene expression profiling
A starting amount of 200 ng high-quality total RNA (equally pooled from two animals) was used to generate cDNA and cRNA with the Illumina TotalPrep RNA Amplification Kit (Illumina Inc., San Diego, CA, USA). The procedure consisted of reverse transcription with an oligo(dT) primer bearing a T7 promoter using Array-Script. The obtained cDNA became a template for in vitro transcription with T7 RNA polymerase and biotin UTP, which generated multiple copies of biotinylated cRNA. The purity and concentration of the cRNA were checked using an ND-1000 Spectrometer. Quality cRNA was then hybridized with Illumina's direct hybridization array kit (Illumina). Each cRNA sample (1.5 μg) was hybridized overnight to the MouseWG-6 BeadChip arrays (Illumina) in a multiple-step procedure according to the manufacturer's instructions; the chips were washed, dried and scanned on the BeadArray Reader (Illumina). Raw microarray data were generated using BeadStudio v3.0 (Illumina). Three biological replicates of the microarrays were prepared per experimental group. A total of 108 Illumina MouseWG-6 v1.1 and 84 Illumina MouseWG-6 v2 microarrays (with probes for approximately 48,000 transcripts) were used in the two experiments. To rule out the effects of injection and fluctuations related to circadian rhythms, we compared the drug effects to saline-treated and naïve animals. The microarray experimental design involved pooling two animals per array and combining three independent arrays per group. To provide an appropriate balance in the whole dataset, groups were equally divided between the array hybridization batches.
Microarray data analysis
Microarray quality control was performed using BeadArray R package v1.10.0. The following parameters were checked on all 192 arrays: number of outliers, number of beads and percent of detected probes. After background subtraction, the data were normalized using quantile normalization and then log2-transformed. The obtained signal was taken as the measure of mRNA abundance derived from the level of gene expression. The results were standardized to reduce the effect of hybridization batches using z-score transformation. Statistical analysis of the results was performed using two-way ANOVA (for the factors drug and time) followed by Bonferroni correction for multiple testing. Alternatively, the FDR (percent FDR) was estimated using the Benjamini and Hochberg method . To obtain drug-versus-saline comparisons, two-way ANOVA was followed by Tukey's post-hoc test. All statistical analyses were performed in R software version 2.8.1 . Gene cross-annotation between the two versions of each microarray was performed automatically based on probe sequence, transcript ID and gene identifier, with some manual corrections.
Hierarchical clustering was performed using the measure of Euclidian distance and average distance linkage methods. The cluster separation was performed according to an arbitrary threshold (h = 13). Several alternative clustering strategies produced similar hierarchical relationships, as shown in Figure 2a. Cluster visualization was performed using dChip software .
Functional annotation, GO enrichment, cell type enrichment and literature mining
The functional annotation analysis tool DAVID 2008 was used to identify over-represented ontologic groups among the gene expression patterns and to group genes into functional classifications . The list of transcripts represented on the Illumina Mouse WG-6 v1.1 microarray was used as a background list. Over-represented GO terms were defined as having at least three transcripts and P < 0.05 under Fisher's exact test. For cell-type enrichment of mRNA, a recently published brain transcriptome database was used . The database of 1267 gene lists was used for the literature enrichment analysis. This included gene lists manually extracted from the published data, as well as a collection of gene sets from the MSigDB database . The statistical significance analysis of transcript enrichment was performed using the ORA algorithm in ErmineJ software . Annotation handling was based on Mouse Gene Symbol IDs (MGI), and all other annotation formats were translated using BioMart . Input data, annotations and the obtained results are included in Additional files 2, 7 and 8.
Identification of transcription factor binding sites enriched in co-regulated transcripts
The identification of over-represented TFBSs was performed using the cREMaG database  with default parameters. Briefly, a 70% conservation threshold and a maximum number of 50 conserved TFBSs in non-coding regions between 5,000 bp upstream and 1,000 bp downstream of the transcriptional start site were used. Functional promoter sequences were identified by alignments between 5' upstream sequences of mouse and human orthologous genes. The identification of TFBSs was performed using the Perl TFBS module and matrices from the JASPAR database . MGI Gene Symbol lists were submitted, and default parameters were used.
Validation of microarray data by qPCR
We performed qPCR measurements for a set of genes representative of the identified gene clusters. Reverse transcription was performed with Omniscript Reverse Transcriptase enzyme (Qiagen) at 37°C for 60 minutes. The reaction was carried out in the presence of the RNase inhibitor rRNAsin (Promega, Madison, WI, USA), and an oligo(dT16) primer (Qiagen) was used to selectively amplify mRNA. qPCR reactions were performed using Assay-On-Demand TaqMan probes (Additional file 3) according to the manufacturer's protocol (Applied Biosystems, Foster City, CA, USA) and were run on an iCycler (Bio-Rad, Foster City, CA, USA). For each reaction, approximately 50 ng of cDNA synthesized from a total RNA template (isolated from an individual animal) was used (n = 4 to 10). To minimize the contribution of contaminating genomic DNA, primers were designed to span exon junctions. Additionally, control reactions without reverse transcription enzyme for each assay were performed. The amplification efficiency for each assay was determined by running a standard dilution curve. The expression of the Hprt1 (hypoxanthine guanine phosphoribosyl transferase 1) transcript, which had a stable mRNA level, was quantified to control for variations in cDNA levels. The cycle threshold values were calculated automatically by iCycler IQ 3.0a software with default parameters. The abundance of RNA was calculated as 2-(threshold cycle).
Measurement of the effects of pharmacological dissection
Further microarray experiments were performed to analyze the effects of selected pharmacological tools on drug-induced gene expression changes. Mean fold changes of drug-induced transcriptional activation for each gene expression pattern with and without the administration of a particular inhibitor or antagonist were compared. The influence was measured as a percentage of inhibition of the drug-induced transcriptional response, with 0% representing no effect and 100% representing complete inhibition. The statistical significance of influence was measured as a comparison of the mean fold change between the drug plus inhibitor/antagonist and saline plus vehicle versus drug plus vehicle and saline plus vehicle groups. All necessary controls, including different vehicles and time points, were included.
Association of gene expression patterns with phenotype
The correlation between the effects of the drugs and behavioral effects in animals was measured using Pearson's method. The mean expression change of each gene was summarized for all time points together for correlation with CPP and for single time points for correlation with locomotor activation.
Protein was extracted from the samples using RIPA buffer. The protein concentration of each sample was determined using the BCA Protein Assay Kit (Sigma-Aldrich). Aliquots of crude extracts (containing 5 to 20 μg of protein) were then subjected to electrophoresis on a 12% SDS-polyacrylamide gel, and proteins were electroblotted onto microporous polyvinylidene difluoride (PVDF) membranes (Roche, Germany). The membranes were blocked for 1 h, washed and incubated overnight with primary antibodies at 4°C. After washing, immunocomplexes were detected using a Chemiluminescence Western Blotting Kit (Mouse/Rabbit, Roche), visualized with a Fujifilm LAS-1000 fluoroimager system and quantified using Image Gauge software (Fujifilm, Tokyo, Japan). For immunoblotting, a rabbit polyclonal antibody raised against: a synthesized non-phosphopeptide derived from human SGK1 around the phosphorylation site of serine 78 (P-P-SP-P-S; Abcam, Cambridge, MA, USA); or a synthetic peptide conjugated to keyhole limpet hemocyanin derived from residue 100 to the carboxyl terminus of Mouse GilZ/TilZ (Abcam) was used. To control for transfer quality, each PVDF blot was stained with Ponceau S.
The frozen brains were cut into 12-μm-thick coronal sections on a cryostat microtome CM 3050S (Leica Microsystems, Germany), and the sections were thaw-mounted on gelatin-chrome alum-coated slides and processed for in situ hybridization. The hybridization procedure was performed as previously described . Briefly, the sections were fixed with 4% paraformaldehyde, washed in PBS and acetylated by incubation with 0.25% acetic anhydrite (in 0.1 M triethanolamine and 0.9% sodium chloride). The sections were then dehydrated using increasing concentrations of ethanol (70 to 100%), treated with chloroform for 5 minutes and rehydrated with decreasing concentrations of ethanol. The sections were hybridized for 15 h at 37°C with oligonucleotide probes complementary to nucleotides 493-536 of the mouse Tsc22d3 cDNA (5'-CAGTTGCTCGGGGCTTGCCAGCGTCTTCAGGAGGGTGTTCTCGC-3'; NM 010286.3) and nucleotides 1682-1725 of the mouse Sgk1 cDNA (5'-TTGATCACAGCTCAGACAGACTGCGGGGATTCCTCTTAGACCTG-3'; NM 011361.1). The probes were labeled with 35S-dATP by the 3'-tailing reaction using terminal transferase (MBI Fermentas, Vilnius, Lithuania). After hybridization, the slices were washed three times for 20 minutes with 1×SSC/50% formamide at 40°C and twice for 50 minutes with 1×SSC at room temperature. Then, the slices were dried and exposed to phosphorimager plates (Fujifilm) for 5 days. The hybridization signal was digitized using a Fujifilm BAS-5000 phosphorimager and Image Reader software.
The animals were deeply anesthetized (pentobarbital, 60 mg/kg i.p.) and perfused transcardially with saline followed by 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Brains were removed, postfixed for 4 h, transferred to PBS and stored at 4°C. Free-floating sections were cut 40-μm thick using a Leica vibratome. For double-immunofluorescence labeling, sections were blocked for 1 h in 5% donkey serum, pH 7.4 (Vector Labs, Burlingame, CA, USA) and then incubated overnight at 4°C in a mixture of primary antibodies. Respective pairs of antibodies included rabbit polyclonal anti-GILZ (1:100; Abcam) or rabbit polyclonal anti-SGK1 (1:400; Abcam) with mouse monoclonal anti-s100-beta (1:1,500; Sigma-Aldrich) or mouse monoclonal anti-NeuN (1:250; Chemicon, Rosemont, IL, USA). After three washes in PBS, double immunofluorescence was revealed by incubating the sections for 2 h at room temperature in a mixture of secondary antibodies: Alexa Fluor 488-conjugated donkey anti-mouse IgG and Alexa Fluor 555-conjugated donkey anti-rabbit IgG (both at 1:750; Molecular Probes Inc., Eugene, OR, USA). The sections were washed three times with PBS, mounted on slides in Vectashield (Vector Labs) and coverslipped. The negative controls were prepared by omitting the primary antibody. The sections were examined using a 63× objective on a confocal microscope (DMRXA2 TCS SP2, Leica Microsystems). The background noise of each confocal image was reduced by averaging four scans per line and four frames per image. To visualize image details, plates were generated adjusting the contrast and brightness of digital images (ImageJ, NIMH).
Primary neuron cultures and transfection
Primary hippocampal and cortical cultures were prepared from embryonic day 16 mouse brains, according to the Banker and Goslin procedure. Cells were plated on coverslips coated with poly-L-lysine (30 μg/ml; Sigma) and laminin (2 μg/ml; Roche) at a density of 500 (hippocampal) or 1,250 (cortical neurons) cells/mm2. Neuronal cultures were grown in Neurobasal medium (Invitrogen) supplemented with B27 (Invitrogen), 0.5 mM glutamine, 12.5 μM glutamate and penicillin/streptomycin mix (Sigma). On the 14th day in vitro (DIV), neurons were transfected with Lipofectamine 2000 (Invitrogen) for 3 days, as previously described . Briefly, for cells growing in a single well of a 24-well dish, 0.9 μg of DNA was mixed with 1.67 μl of Lipofectamine 2000 in 100 μl of Neurobasal medium and incubated for 30 minutes. During the incubation time, fresh culture media were prepared, mixed half and half with old media and split into two equal aliquots. The first aliquot was added to the cells, and the second was saved during the transfection period. Next, complexes of DNA with Lipofectamine 2000 were added to the cells and incubated for 4 h at 37°C, 5% CO2. Finally, cells were washed twice with Neurobasal medium and incubated in the saved culture media. The pSUPER vector  and β-actin-GFP  mammalian expression plasmids have been described. GILZ shRNAs were designed against mouse GILZ (Tsc22d3) mRNA (EntrezGene ID: 14605) targeting nucleotides 383-401, 385-407 and 437-459, respectively. SGK1 shRNAs targeted nucleotides 265-283, 719-737, 975-993 and 1008-1026 of Sgk1 (EntrezGene ID: 20393), respectively. All shRNAs were cloned into pSUPER. In RNA inyerference experiments, the mix of shRNAs encoding plasmids and β-actin-GFP were cotransfected at a 3:1 ratio.
Immunocytochemistry of in vitrocultured neurons
For immunofluorescent staining of transfected GFP, neurons were fixed with 4% paraformaldehyde containing 4% sucrose in PBS for 10 minutes at room temperature. After fixation, cells were washed three times with PBS for 5 minutes at room temperature and incubated with primary antibody in GDB buffer (0.2% gelatin, 0.8 M NaCl, 0.5% Triton X-100 and 30 mM phosphate buffer, pH 7.4) overnight at 4°C. Cells were then washed three times with PBS for 10 minutes at room temperature. Secondary antibodies were applied in GDB for 1 h at room temperature and washed out by three 10-minutes PBS washes. The secondary antibodies were rabbit anti-GFP (Medical and Biological Laboratories Co. Ltd., Nagoya, Japan) and anti-rabbit Alexa Fluor 488-conjugated secondary antibody (Invitrogen). Confocal images of cells were obtained with sequential acquisition settings at the maximal 1,024 × 1,024 pixel resolution of the Zeiss LSM 5 Exciter microscope (Carl Zeiss, Germany). Each image was a z-series of images, and each was averaged two times. The obtained stacks were directly analyzed using Imaris v6.3.1 (Bitplane AG, Zurich, Switzerland). The changes in spine length, diameter and volume were quantified based on the three-dimensional reconstructions computed by Imaris.
Regulatory network modeling
Microarray data were submitted to the NCBI Gene Expression Omnibus (GEO) under accession number [GEO:GSE15774].
conditioned place preference
cyclic AMP-response element binding protein
corticotrophin-releasing factor receptor 1
dopamine receptor 1
extracellular signal-regulated kinase
false discovery rate
green fluorescent protein
immediate early gene
short hairpin RNA
serum response factor
transcription factor binding site.
We thank J Rodriguez Parkitna for helpful discussions and critical comments on the manuscript and J Kubik, and W Mlynarski for technical assistance. This work was supported by EU grant LSHM-CT-2004-005166, EU grant LSHM-CT-2007-037669, MSHE grants PBZ-MNiI-2/1/2005, N N405 274137 and No. 2 P05A 072 29. JJ was supported by Polish-Norwegian Research Fund grant no. PNRF-96-AI-1/07. IC was supported by 7FP EU grant 223276 "NeuroGSK3".
- Nutt D, King LA, Saulsbury W, Blakemore C: Development of a rational scale to assess the harm of drugs of potential misuse. Lancet. 2007, 369: 1047-1053. 10.1016/S0140-6736(07)60464-4.PubMedGoogle Scholar
- Nugent FS, Penick EC, Kauer JA: Opioids block long-term potentiation of inhibitory synapses. Nature. 2007, 446: 1086-1090. 10.1038/nature05726.PubMedGoogle Scholar
- Ungless MA, Whistler JL, Malenka RC, Bonci A: Single cocaine exposure in vivo induces long-term potentiation in dopamine neurons. Nature. 2001, 411: 583-587. 10.1038/35079077.PubMedGoogle Scholar
- Hyman SE, Malenka RC, Nestler EJ: Neural mechanisms of addiction: the role of reward-related learning and memory. Annu Rev Neurosci. 2006, 29: 565-598. 10.1146/annurev.neuro.29.051605.113009.PubMedGoogle Scholar
- Nguyen PV, Abel T, Kandel ER: Requirement of a critical period of transcription for induction of a late phase of LTP. Science. 1994, 265: 1104-1107. 10.1126/science.8066450.PubMedGoogle Scholar
- Gerdeman GL, Partridge JG, Lupica CR, Lovinger DM: It could be habit forming: drugs of abuse and striatal synaptic plasticity. Trends Neurosci. 2003, 26: 184-192. 10.1016/S0166-2236(03)00065-1.PubMedGoogle Scholar
- Devan BD, White NM: Parallel information processing in the dorsal striatum: relation to hippocampal function. J Neurosci. 1999, 19: 2789-2798.PubMedGoogle Scholar
- McBride WJ, Murphy JM, Ikemoto S: Localization of brain reinforcement mechanisms: intracranial self-administration and intracranial place-conditioning studies. Behav Brain Res. 1999, 101: 129-152. 10.1016/S0166-4328(99)00022-4.PubMedGoogle Scholar
- Dickson PR, Lang CG, Hinton SC, Kelley AE: Oral stereotypy induced by amphetamine microinjection into striatum: an anatomical mapping study. Neuroscience. 1994, 61: 81-91. 10.1016/0306-4522(94)90062-0.PubMedGoogle Scholar
- Kelley AE, Delfs JM: Dopamine and conditioned reinforcement. I. Differential effects of amphetamine microinjections into striatal subregions. Psychopharmacology (Berl). 1991, 103: 187-196. 10.1007/BF02244202.Google Scholar
- Di Chiara G, Imperato A: Drugs abused by humans preferentially increase synaptic dopamine concentrations in the mesolimbic system of freely moving rats. Proc Natl Acad Sci USA. 1988, 85: 5274-5278. 10.1073/pnas.85.14.5274.PubMedPubMed CentralGoogle Scholar
- Kilty JE, Lorang D, Amara SG: Cloning and expression of a cocaine-sensitive rat dopamine transporter. Science. 1991, 254: 578-579. 10.1126/science.1948035.PubMedGoogle Scholar
- Shimada S, Kitayama S, Lin CL, Patel A, Nanthakumar E, Gregor P, Kuhar M, Uhl G: Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA. Science. 1991, 254: 576-578. 10.1126/science.1948034.PubMedGoogle Scholar
- Johnson SW, North RA: Opioids excite dopamine neurons by hyperpolarization of local interneurons. J Neurosci. 1992, 12: 483-488.PubMedGoogle Scholar
- Pert CB, Kuhar MJ, Snyder SH: Opiate receptor: autoradiographic localization in rat brain. Proc Natl Acad Sci USA. 1976, 73: 3729-3733. 10.1073/pnas.73.10.3729.PubMedPubMed CentralGoogle Scholar
- Buck KJ: Molecular genetic analysis of the role of GABAergic systems in the behavioral and cellular actions of alcohol. Behav Genet. 1996, 26: 313-323. 10.1007/BF02359387.PubMedGoogle Scholar
- Pidoplichko VI, DeBiasi M, Williams JT, Dani JA: Nicotine activates and desensitizes midbrain dopamine neurons. Nature. 1997, 390: 401-404. 10.1038/37120.PubMedGoogle Scholar
- Rice ME, Cragg SJ: Nicotine amplifies reward-related dopamine signals in striatum. Nat Neurosci. 2004, 7: 583-584. 10.1038/nn1244.PubMedGoogle Scholar
- Koob GF, Le Moal M: Drug addiction, dysregulation of reward, and allostasis. Neuropsychopharmacology. 2001, 24: 97-129. 10.1016/S0893-133X(00)00195-0.PubMedGoogle Scholar
- Kauer JA, Malenka RC: Synaptic plasticity and addiction. Nat Rev Neurosci. 2007, 8: 844-858. 10.1038/nrn2234.PubMedGoogle Scholar
- Rhodes JS, Crabbe JC: Gene expression induced by drugs of abuse. Curr Opin Pharmacol. 2005, 5: 26-33. 10.1016/j.coph.2004.12.001.PubMedGoogle Scholar
- Thomas DM, Francescutti-Verbeem DM, Liu X, Kuhn DM: Identification of differentially regulated transcripts in mouse striatum following methamphetamine treatment--an oligonucleotide microarray approach. J Neurochem. 2004, 88: 380-393.PubMedGoogle Scholar
- Yuferov V, Kroslak T, Laforge KS, Zhou Y, Ho A, Kreek MJ: Differential gene expression in the rat caudate putamen after "binge" cocaine administration: advantage of triplicate microarray analysis. Synapse. 2003, 48: 157-169. 10.1002/syn.10198.PubMedGoogle Scholar
- Befort K, Filliol D, Ghate A, Darcq E, Matifas A, Muller J, Lardenois A, Thibault C, Dembele D, Le Merrer J, et al: Mu-opioid receptor activation induces transcriptional plasticity in the central extended amygdala. Eur J Neurosci. 2008, 27: 2973-2984. 10.1111/j.1460-9568.2008.06273.x.PubMedGoogle Scholar
- Rodriguez Parkitna JM, Bilecki W, Mierzejewski P, Stefanski R, Ligeza A, Bargiela A, Ziolkowska B, Kostowski W, Przewlocki R: Effects of morphine on gene expression in the rat amygdala. J Neurochem. 2004, 91: 38-48. 10.1111/j.1471-4159.2004.02697.x.PubMedGoogle Scholar
- Kerns RT, Ravindranathan A, Hassan S, Cage MP, York T, Sikela JM, Williams RW, Miles MF: Ethanol-responsive brain region expression networks: implications for behavioral responses to acute ethanol in DBA/2J versus C57BL/6J mice. J Neurosci. 2005, 25: 2255-2266. 10.1523/JNEUROSCI.4372-04.2005.PubMedGoogle Scholar
- McClung CA, Nestler EJ, Zachariou V: Regulation of gene expression by chronic morphine and morphine withdrawal in the locus ceruleus and ventral tegmental area. J Neurosci. 2005, 25: 6005-6015. 10.1523/JNEUROSCI.0062-05.2005.PubMedGoogle Scholar
- Ammon S, Mayer P, Riechert U, Tischmeyer H, Hollt V: Microarray analysis of genes expressed in the frontal cortex of rats chronically treated with morphine and after naloxone precipitated withdrawal. Brain Res Mol Brain Res. 2003, 112: 113-125. 10.1016/S0169-328X(03)00057-3.PubMedGoogle Scholar
- Lamb J, Crawford ED, Peck D, Modell JW, Blat IC, Wrobel MJ, Lerner J, Brunet JP, Subramanian A, Ross KN, et al: The Connectivity Map: using gene-expression signatures to connect small molecules, genes, and disease. Science. 2006, 313: 1929-1935. 10.1126/science.1132939.PubMedGoogle Scholar
- Dobrin R, Zhu J, Molony C, Argman C, Parrish ML, Carlson S, Allan MF, Pomp D, Schadt EE: Multi-tissue coexpression networks reveal unexpected subnetworks associated with disease. Genome Biol. 2009, 10: R55-10.1186/gb-2009-10-5-r55.PubMedPubMed CentralGoogle Scholar
- Emilsson V, Thorleifsson G, Zhang B, Leonardson AS, Zink F, Zhu J, Carlson S, Helgason A, Walters GB, Gunnarsdottir S, et al: Genetics of gene expression and its effect on disease. Nature. 2008, 452: 423-428. 10.1038/nature06758.PubMedGoogle Scholar
- Claverie JM: Computational methods for the identification of differential and coordinated gene expression. Hum Mol Genet. 1999, 8: 1821-1832. 10.1093/hmg/8.10.1821.PubMedGoogle Scholar
- Choy E, Yelensky R, Bonakdar S, Plenge RM, Saxena R, De Jager PL, Shaw SY, Wolfish CS, Slavik JM, Cotsapas C, et al: Genetic analysis of human traits in vitro: drug response and gene expression in lymphoblastoid cell lines. PLoS Genet. 2008, 4: e1000287-10.1371/journal.pgen.1000287.PubMedPubMed CentralGoogle Scholar
- Lan H, Chen M, Flowers JB, Yandell BS, Stapleton DS, Mata CM, Mui ET, Flowers MT, Schueler KL, Manly KF, et al: Combined expression trait correlations and expression quantitative trait locus mapping. PLoS Genet. 2006, 2: e6-10.1371/journal.pgen.0020006.PubMedPubMed CentralGoogle Scholar
- Solecki W, Turek A, Kubik J, Przewlocki R: Motivational effects of opiates in conditioned place preference and aversion paradigm-a study in three inbred strains of mice. Psychopharmacology (Berl). 2009, 207: 245-255.Google Scholar
- Jhoo WK, Shin EJ, Lee YH, Cheon MA, Oh KW, Kang SY, Lee C, Yi BC, Kim HC: Dual effects of dextromethorphan on cocaine-induced conditioned place preference in mice. Neurosci Lett. 2000, 288: 76-80. 10.1016/S0304-3940(00)01188-5.PubMedGoogle Scholar
- Takamatsu Y, Yamamoto H, Ogai Y, Hagino Y, Markou A, Ikeda K: Fluoxetine as a potential pharmacotherapy for methamphetamine dependence: studies in mice. Ann N Y Acad Sci. 2006, 1074: 295-302. 10.1196/annals.1369.026.PubMedGoogle Scholar
- Rice RW: Analyzing tables of statistical tests. Evolution. 1989, 43: 223-225. 10.2307/2409177.Google Scholar
- Benjamini Y, Hochberg Y: Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the Royal Statistical Society Series B (Methodological). 1995, 57: 289-300.Google Scholar
- Jayanthi S, McCoy MT, Beauvais G, Ladenheim B, Gilmore K, Wood W, Becker K, Cadet JL: Methamphetamine induces dopamine D1 receptor-dependent endoplasmic reticulum stress-related molecular events in the rat striatum. PLoS One. 2009, 4: e6092-10.1371/journal.pone.0006092.PubMedPubMed CentralGoogle Scholar
- Lemberger T, Parkitna JR, Chai M, Schutz G, Engblom D: CREB has a context-dependent role in activity-regulated transcription and maintains neuronal cholesterol homeostasis. FASEB J. 2008, 22: 2872-2879. 10.1096/fj.08-107888.PubMedGoogle Scholar
- Ryan JC, Morey JS, Ramsdell JS, Van Dolah FM: Acute phase gene expression in mice exposed to the marine neurotoxin domoic acid. Neuroscience. 2005, 136: 1121-1132. 10.1016/j.neuroscience.2005.08.047.PubMedGoogle Scholar
- Impey S, McCorkle SR, Cha-Molstad H, Dwyer JM, Yochum GS, Boss JM, McWeeney S, Dunn JJ, Mandel G, Goodman RH: Defining the CREB regulon: a genome-wide analysis of transcription factor regulatory regions. Cell. 2004, 119: 1041-1054.PubMedGoogle Scholar
- Sanchis-Segura C, Lopez-Atalaya JP, Barco A: Selective boosting of transcriptional and behavioral responses to drugs of abuse by histone deacetylase inhibition. Neuropsychopharmacology. 2009, 34: 2642-2654. 10.1038/npp.2009.125.PubMedGoogle Scholar
- Korostynski M, Piechota M, Kaminska D, Solecki W, Przewlocki R: Morphine effects on striatal transcriptome in mice. Genome Biol. 2007, 8: R128-10.1186/gb-2007-8-6-r128.PubMedPubMed CentralGoogle Scholar
- Treadwell JA, Singh SM: Microarray analysis of mouse brain gene expression following acute ethanol treatment. Neurochem Res. 2004, 29: 357-369. 10.1023/B:NERE.0000013738.06437.a6.PubMedGoogle Scholar
- Sato H, Horikawa Y, Iizuka K, Sakurai N, Tanaka T, Shihara N, Oshima A, Takeda J, Mikuni M: Large-scale analysis of glucocorticoid target genes in rat hypothalamus. J Neurochem. 2008, 106: 805-814. 10.1111/j.1471-4159.2008.05489.x.PubMedGoogle Scholar
- Ramanan N, Shen Y, Sarsfield S, Lemberger T, Schutz G, Linden DJ, Ginty DD: SRF mediates activity-induced gene expression and synaptic plasticity but not neuronal viability. Nat Neurosci. 2005, 8: 759-767. 10.1038/nn1462.PubMedGoogle Scholar
- Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H, Ross SE, Mostoslavsky R, Cohen HY, et al: Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Science. 2004, 303: 2011-2015. 10.1126/science.1094637.PubMedGoogle Scholar
- Valjent E, Corvol JC, Pages C, Besson MJ, Maldonado R, Caboche J: Involvement of the extracellular signal-regulated kinase cascade for cocaine-rewarding properties. J Neurosci. 2000, 20: 8701-8709.PubMedGoogle Scholar
- Mierzejewski P, Siemiatkowski M, Radwanska K, Szyndler J, Bienkowski P, Stefanski R, Kaczmarek L, Kostowski W: Cycloheximide impairs acquisition but not extinction of cocaine self-administration. Neuropharmacology. 2006, 51: 367-373. 10.1016/j.neuropharm.2006.04.001.PubMedGoogle Scholar
- Marinelli M, Aouizerate B, Barrot M, Le Moal M, Piazza PV: Dopamine-dependent responses to morphine depend on glucocorticoid receptors. Proc Natl Acad Sci USA. 1998, 95: 7742-7747. 10.1073/pnas.95.13.7742.PubMedPubMed CentralGoogle Scholar
- Funk CK, Zorrilla EP, Lee MJ, Rice KC, Koob GF: Corticotropin-releasing factor 1 antagonists selectively reduce ethanol self-administration in ethanol-dependent rats. Biol Psychiatry. 2007, 61: 78-86. 10.1016/j.biopsych.2006.03.063.PubMedPubMed CentralGoogle Scholar
- Kumar A, Choi KH, Renthal W, Tsankova NM, Theobald DE, Truong HT, Russo SJ, Laplant Q, Sasaki TS, Whistler KN, et al: Chromatin remodeling is a key mechanism underlying cocaine-induced plasticity in striatum. Neuron. 2005, 48: 303-314. 10.1016/j.neuron.2005.09.023.PubMedGoogle Scholar
- Jaworski J, Kapitein LC, Gouveia SM, Dortland BR, Wulf PS, Grigoriev I, Camera P, Spangler SA, Di Stefano P, Demmers J, et al: Dynamic microtubules regulate dendritic spine morphology and synaptic plasticity. Neuron. 2009, 61: 85-100. 10.1016/j.neuron.2008.11.013.PubMedGoogle Scholar
- Jaworski J, Spangler S, Seeburg DP, Hoogenraad CC, Sheng M: Control of dendritic arborization by the phosphoinositide-3'-kinase-Akt-mammalian target of rapamycin pathway. J Neurosci. 2005, 25: 11300-11312. 10.1523/JNEUROSCI.2270-05.2005.PubMedGoogle Scholar
- Greenberg ME, Ziff EB, Greene LA: Stimulation of neuronal acetylcholine receptors induces rapid gene transcription. Science. 1986, 234: 80-83. 10.1126/science.3749894.PubMedGoogle Scholar
- Guzowski JF, Timlin JA, Roysam B, McNaughton BL, Worley PF, Barnes CA: Mapping behaviorally relevant neural circuits with immediate-early gene expression. Curr Opin Neurobiol. 2005, 15: 599-606. 10.1016/j.conb.2005.08.018.PubMedGoogle Scholar
- Morgan JI, Cohen DR, Hempstead JL, Curran T: Mapping patterns of c-fos expression in the central nervous system after seizure. Science. 1987, 237: 192-197. 10.1126/science.3037702.PubMedGoogle Scholar
- Graybiel AM, Moratalla R, Robertson HA: Amphetamine and cocaine induce drug-specific activation of the c-fos gene in striosome-matrix compartments and limbic subdivisions of the striatum. Proc Natl Acad Sci USA. 1990, 87: 6912-6916. 10.1073/pnas.87.17.6912.PubMedPubMed CentralGoogle Scholar
- Hope B, Kosofsky B, Hyman SE, Nestler EJ: Regulation of immediate early gene expression and AP-1 binding in the rat nucleus accumbens by chronic cocaine. Proc Natl Acad Sci USA. 1992, 89: 5764-5768. 10.1073/pnas.89.13.5764.PubMedPubMed CentralGoogle Scholar
- Konradi C, Cole RL, Heckers S, Hyman SE: Amphetamine regulates gene expression in rat striatum via transcription factor CREB. J Neurosci. 1994, 14: 5623-5634.PubMedGoogle Scholar
- Janssen-Timmen U, Lemaire P, Mattei MG, Revelant O, Charnay P: Structure, chromosome mapping and regulation of the mouse zinc-finger gene Krox-24; evidence for a common regulatory pathway for immediate-early serum-response genes. Gene. 1989, 80: 325-336. 10.1016/0378-1119(89)90296-5.PubMedGoogle Scholar
- Radwanska K, Caboche J, Kaczmarek L: Extracellular signal-regulated kinases (ERKs) modulate cocaine-induced gene expression in the mouse amygdala. Eur J Neurosci. 2005, 22: 939-948. 10.1111/j.1460-9568.2005.04286.x.PubMedGoogle Scholar
- Carlezon WA, Duman RS, Nestler EJ: The many faces of CREB. Trends Neurosci. 2005, 28: 436-445. 10.1016/j.tins.2005.06.005.PubMedGoogle Scholar
- Dong Y, Green T, Saal D, Marie H, Neve R, Nestler EJ, Malenka RC: CREB modulates excitability of nucleus accumbens neurons. Nat Neurosci. 2006, 9: 475-477. 10.1038/nn1661.PubMedGoogle Scholar
- Lin Y, Bloodgood BL, Hauser JL, Lapan AD, Koon AC, Kim TK, Hu LS, Malik AN, Greenberg ME: Activity-dependent regulation of inhibitory synapse development by Npas4. Nature. 2008, 455: 1198-1204. 10.1038/nature07319.PubMedPubMed CentralGoogle Scholar
- Tappe A, Klugmann M, Luo C, Hirlinger D, Agarwal N, Benrath J, Ehrengruber MU, During MJ, Kuner R: Synaptic scaffolding protein Homer1a protects against chronic inflammatory pain. Nat Med. 2006, 12: 677-681. 10.1038/nm1406.PubMedGoogle Scholar
- Chowdhury S, Shepherd JD, Okuno H, Lyford G, Petralia RS, Plath N, Kuhl D, Huganir RL, Worley PF: Arc/Arg3.1 interacts with the endocytic machinery to regulate AMPA receptor trafficking. Neuron. 2006, 52: 445-459. 10.1016/j.neuron.2006.08.033.PubMedPubMed CentralGoogle Scholar
- Rial Verde EM, Lee-Osbourne J, Worley PF, Malinow R, Cline HT: Increased expression of the immediate-early gene arc/arg3.1 reduces AMPA receptor-mediated synaptic transmission. Neuron. 2006, 52: 461-474. 10.1016/j.neuron.2006.09.031.PubMedPubMed CentralGoogle Scholar
- Le F, Wilce P, Cassady I, Hume D, Shanley B: Acute administration of ethanol suppresses pentylenetetrazole-induced c-fos expression in rat brain. Neurosci Lett. 1990, 120: 271-274. 10.1016/0304-3940(90)90057-G.PubMedGoogle Scholar
- Ryabinin AE, Melia KR, Cole M, Bloom FE, Wilson MC: Alcohol selectively attenuates stress-induced c-fos expression in rat hippocampus. J Neurosci. 1995, 15: 721-730.PubMedGoogle Scholar
- Torres G: Acute administration of alcohol blocks cocaine-induced striatal c-fos immunoreactivity protein in the rat. Synapse. 1994, 18: 161-167. 10.1002/syn.890180208.PubMedGoogle Scholar
- Ellis FW: Effect of ethanol on plasma corticosterone levels. J Pharmacol Exp Ther. 1966, 153: 121-127.PubMedGoogle Scholar
- George R, Way EL: Studies on the mechanism of pituitary-adrenal activation by morphine. Br J Pharmacol Chemother. 1955, 10: 260-264.PubMedPubMed CentralGoogle Scholar
- de Jong IE, de Kloet ER: Glucocorticoids and vulnerability to psychostimulant drugs: toward substrate and mechanism. Ann N Y Acad Sci. 2004, 1018: 192-198. 10.1196/annals.1296.022.PubMedGoogle Scholar
- Marinelli M, Piazza PV: Interaction between glucocorticoid hormones, stress and psychostimulant drugs. Eur J Neurosci. 2002, 16: 387-394. 10.1046/j.1460-9568.2002.02089.x.PubMedGoogle Scholar
- Heilig M, Koob GF: A key role for corticotropin-releasing factor in alcohol dependence. Trends Neurosci. 2007, 30: 399-406. 10.1016/j.tins.2007.06.006.PubMedPubMed CentralGoogle Scholar
- Goeders NE: The impact of stress on addiction. Eur Neuropsychopharmacol. 2003, 13: 435-441. 10.1016/j.euroneuro.2003.08.004.PubMedGoogle Scholar
- Yachi K, Inoue K, Tanaka H, Yoshikawa H, Tohyama M: Localization of glucocorticoid-induced leucine zipper (GILZ) expressing neurons in the central nervous system and its relationship to the stress response. Brain Res. 2007, 1159: 141-147. 10.1016/j.brainres.2007.05.024.PubMedGoogle Scholar
- Chipkin SR, van Bueren A, Bercel E, Garrison CR, McCall AL: Effects of dexamethasone in vivo and in vitro on hexose transport in brain microvasculature. Neurochem Res. 1998, 23: 645-652. 10.1023/A:1022434721114.PubMedGoogle Scholar
- Wagner CA, Ott M, Klingel K, Beck S, Melzig J, Friedrich B, Wild KN, Broer S, Moschen I, Albers A, et al: Effects of the serine/threonine kinase SGK1 on the epithelial Na(+) channel (ENaC) and CFTR: implications for cystic fibrosis. Cell Physiol Biochem. 2001, 11: 209-218. 10.1159/000051935.PubMedGoogle Scholar
- Yoshida K, Shimizugawa T, Ono M, Furukawa H: Angiopoietin-like protein 4 is a potent hyperlipidemia-inducing factor in mice and inhibitor of lipoprotein lipase. J Lipid Res. 2002, 43: 1770-1772. 10.1194/jlr.C200010-JLR200.PubMedGoogle Scholar
- Zhang H, Xiong Y, Beach D: Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes. Mol Biol Cell. 1993, 4: 897-906.PubMedPubMed CentralGoogle Scholar
- Zhou Y, Spangler R, Schlussman SD, Yuferov VP, Sora I, Ho A, Uhl GR, Kreek MJ: Effects of acute "binge" cocaine on preprodynorphin, preproenkephalin, proopiomelanocortin, and corticotropin-releasing hormone receptor mRNA levels in the striatum and hypothalamic-pituitary-adrenal axis of mu-opioid receptor knockout mice. Synapse. 2002, 45: 220-229. 10.1002/syn.10101.PubMedGoogle Scholar
- Yang-Yen HF, Chambard JC, Sun YL, Smeal T, Schmidt TJ, Drouin J, Karin M: Transcriptional interference between c-Jun and the glucocorticoid receptor: mutual inhibition of DNA binding due to direct protein-protein interaction. Cell. 1990, 62: 1205-1215. 10.1016/0092-8674(90)90396-V.PubMedGoogle Scholar
- Stehle JH, Foulkes NS, Molina CA, Simonneaux V, Pevet P, Sassone-Corsi P: Adrenergic signals direct rhythmic expression of transcriptional repressor CREM in the pineal gland. Nature. 1993, 365: 314-320. 10.1038/365314a0.PubMedGoogle Scholar
- Tatro ET, Everall IP, Kaul M, Achim CL: Modulation of glucocorticoid receptor nuclear translocation in neurons by immunophilins FKBP51 and FKBP52: implications for major depressive disorder. Brain Res. 2009, 1286: 1-12. 10.1016/j.brainres.2009.06.036.PubMedPubMed CentralGoogle Scholar
- Mendelson JH, Sholar MB, Goletiani N, Siegel AJ, Mello NK: Effects of low- and high-nicotine cigarette smoking on mood states and the HPA axis in men. Neuropsychopharmacology. 2005, 30: 1751-1763. 10.1038/sj.npp.1300753.PubMedPubMed CentralGoogle Scholar
- Piazza PV, Le Moal M: The role of stress in drug self-administration. Trends Pharmacol Sci. 1998, 19: 67-74. 10.1016/S0165-6147(97)01115-2.PubMedGoogle Scholar
- Stewart J: Pathways to relapse: the neurobiology of drug- and stress-induced relapse to drug-taking. J Psychiatry Neurosci. 2000, 25: 125-136.PubMedPubMed CentralGoogle Scholar
- Le Moal M: Drug abuse: vulnerability and transition to addiction. Pharmacopsychiatry. 2009, 42 (Suppl 1): S42-55. 10.1055/s-0029-1216355.PubMedGoogle Scholar
- Mantsch JR, Saphier D, Goeders NE: Corticosterone facilitates the acquisition of cocaine self-administration in rats: opposite effects of the type II glucocorticoid receptor agonist dexamethasone. J Pharmacol Exp Ther. 1998, 287: 72-80.PubMedGoogle Scholar
- Luca F, Kashyap S, Southard C, Zou M, Witonsky D, Di Rienzo A, Conzen SD: Adaptive variation regulates the expression of the human SGK1 gene in response to stress. PLoS Genet. 2009, 5: e1000489-10.1371/journal.pgen.1000489.PubMedPubMed CentralGoogle Scholar
- Ma YL, Tsai MC, Hsu WL, Lee EH: SGK protein kinase facilitates the expression of long-term potentiation in hippocampal neurons. Learn Mem. 2006, 13: 114-118. 10.1101/lm.179206.PubMedGoogle Scholar
- Park JS, Onodera T, Nishimura S, Thompson RF, Itohara S: Molecular evidence for two-stage learning and partial laterality in eyeblink conditioning of mice. Proc Natl Acad Sci USA. 2006, 103: 5549-5554. 10.1073/pnas.0601150103.PubMedPubMed CentralGoogle Scholar
- David S, Stegenga SL, Hu P, Xiong G, Kerr E, Becker KB, Venkatapathy S, Warrington JA, Kalb RG: Expression of serum- and glucocorticoid-inducible kinase is regulated in an experience-dependent manner and can cause dendrite growth. J Neurosci. 2005, 25: 7048-7053. 10.1523/JNEUROSCI.0006-05.2005.PubMedGoogle Scholar
- Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, et al: Bioconductor: open software development for computational biology and bioinformatics. Genome Biol. 2004, 5: R80-10.1186/gb-2004-5-10-r80.PubMedPubMed CentralGoogle Scholar
- Li C, Wong WH: Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection. Proc Natl Acad Sci USA. 2001, 98: 31-36. 10.1073/pnas.011404098.PubMedPubMed CentralGoogle Scholar
- Huang da W, Sherman BT, Tan Q, Collins JR, Alvord WG, Roayaei J, Stephens R, Baseler MW, Lane HC, Lempicki RA: The DAVID Gene Functional Classification Tool: a novel biological module-centric algorithm to functionally analyze large gene lists. Genome Biol. 2007, 8: R183-10.1186/gb-2007-8-9-r183.PubMedPubMed CentralGoogle Scholar
- Cahoy JD, Emery B, Kaushal A, Foo LC, Zamanian JL, Christopherson KS, Xing Y, Lubischer JL, Krieg PA, Krupenko SA, et al: A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function. J Neurosci. 2008, 28: 264-278. 10.1523/JNEUROSCI.4178-07.2008.PubMedGoogle Scholar
- Lee HK, Braynen W, Keshav K, Pavlidis P: ErmineJ: tool for functional analysis of gene expression data sets. BMC Bioinformatics. 2005, 6: 269-10.1186/1471-2105-6-269.PubMedPubMed CentralGoogle Scholar
- Durinck S, Spellman PT, Birney E, Huber W: Mapping identifiers for the integration of genomic datasets with the R/Bioconductor package biomaRt. Nat Protoc. 2009, 4: 1184-1191. 10.1038/nprot.2009.97.PubMedPubMed CentralGoogle Scholar
- Goeders NE: Stress and cocaine addiction. J Pharmacol Exp Ther. 2002, 301: 785-789. 10.1124/jpet.301.3.785.PubMedGoogle Scholar
- Sandelin A, Alkema W, Engstrom P, Wasserman WW, Lenhard B: JASPAR: an open-access database for eukaryotic transcription factor binding profiles. Nucleic Acids Res. 2004, 32: D91-94. 10.1093/nar/gkh012.PubMedPubMed CentralGoogle Scholar
- Ziolkowska B, Gieryk A, Bilecki W, Wawrzczak-Bargiela A, Wedzony K, Chocyk A, Danielson PE, Thomas EA, Hilbush BS, Sutcliffe JG, Przewlocki R: Regulation of alpha-synuclein expression in limbic and motor brain regions of morphine-treated mice. J Neurosci. 2005, 25: 4996-5003. 10.1523/JNEUROSCI.4376-04.2005.PubMedGoogle Scholar
- Brummelkamp TR, Bernards R, Agami R: A system for stable expression of short interfering RNAs in mammalian cells. Science. 2002, 296: 550-553. 10.1126/science.1068999.PubMedGoogle Scholar
- Rodriguez Parkitna J, Bilbao A, Rieker C, Engblom D, Piechota M, Nordheim A, Spanagel R, Schutz G: Loss of the serum response factor in the dopamine system leads to hyperactivity. FASEB J. 2010,Google Scholar
- Chen Y, Park S, Li Y, Missov E, Hou M, Han X, Hall JL, Miller LW, Bache RJ: Alterations of gene expression in failing myocardium following left ventricular assist device support. Physiol Genomics. 2003, 14: 251-260.PubMedGoogle Scholar
- Hassan HE, Myers AL, Lee IJ, Chen H, Coop A, Eddington ND: Regulation of gene expression in brain tissues of rats repeatedly treated by the highly abused opioid agonist, oxycodone: microarray profiling and gene mapping analysis. Drug Metab Dispos. 38: 157-167. 10.1124/dmd.109.029199.
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