Combined analysis reveals a core set of cycling genes
© Lu et al.; licensee BioMed Central Ltd. 2007
Received: 30 March 2007
Accepted: 24 July 2007
Published: 24 July 2007
Global transcript levels throughout the cell cycle have been characterized using microarrays in several species. Early analysis of these experiments focused on individual species. More recently, a number of studies have concluded that a surprisingly small number of genes conserved in two or more species are periodically transcribed in these species. Combining and comparing data from multiple species is challenging because of noise in expression data, the different synchronization and scoring methods used, and the need to determine an accurate set of homologs.
To solve these problems, we developed and applied a new algorithm to analyze expression data from multiple species simultaneously. Unlike previous studies, we find that more than 20% of cycling genes in budding yeast have cycling homologs in fission yeast and 5% to 7% of cycling genes in each of four species have cycling homologs in all other species. These conserved cycling genes display much stronger cell cycle characteristics in several complementary high throughput datasets.
Essentiality analysis for yeast and human genes confirms these findings. Motif analysis indicates conservation in the corresponding regulatory mechanisms. Gene Ontology analysis and analysis of the genes in the conserved sets sheds light on the evolution of specific subfunctions within the cell cycle.
Our results indicate that the conservation in cyclic expression patterns is much greater than was previously thought. These genes are highly enriched for most cell cycle categories, and a large percentage of them are essential, supporting our claim that cross-species analysis can identify the core set of cycling genes.
The cell cycle is a series of linked, fundamentally conserved processes that result in high-fidelity cell duplication. Global transcript levels throughout the cell cycle have been characterized using microarray expression data in several species. These include humans , budding and fission yeast [2–6], plants , and bacteria . Early analysis of these experiments focused on individual species. Hundreds of genes have been identified whose transcripts oscillate during the cell cycle, and in budding yeast it is estimated that 15% of all genes are subject to this type of control. Despite this large cross-species effort, a number of studies have concluded that a surprisingly small number of genes conserved in two or more species are periodically transcribed in these species. Rustici and coworkers  compared fission and budding yeast expression data. Dyczkowski and Vingron  compared three lists of cycling genes (budding and fission yeast and human), and Jensen and colleagues  added a fourth species (Arabidopsis). All three studies concluded that periodicity at the transcript level was conserved across species in only a small number of cases.
When comparing cyclic expression patterns across species, researchers face several challenges. In some cases the lists derived for each species were generated using different expression analysis methods. For example, the scoring methods used by Spellman  and Rustici  and their colleagues are different, which makes direct comparison problematic. Another challenge arises when determining the set of homologs between the species being analyzed. Although using curated databases results in a more accurate set of conserved pairs, this analysis is limited to a small (and sometimes biased) set of genes. In addition, the binary assignment (ortholog or not) in databases cannot account for more complex similarity measures, which are often represented using a more continuous value (for example, BLAST e-value). Relying on the actual strength of homology may help when looking for conserved sets. Finally, expression data are noisy. Repeated experiments, even within the same species, often result in relatively low agreement , and differences between species may be even more problematic because radically different synchronization procedures must be used . Any combination of the above may bias the analysis and prevent the identification of an accurate set of conserved cycling genes.
Here we use an algorithm that analyzes data from all species concurrently. This differs from previous methods that performed this analysis separately for each species and then looked at the overlap. Our method overcomes many of the obstacles discussed above. We use the same scoring method for all species, and include parameters that allow a gene in one species to influence the score of a homologous gene (in either the same or in another species). These parameters are continuous and depend on the similarity between the genes. They allow for one to many and for many to many mappings between genes; they also allow higher quality expression data in one species to improve the quality of the data for other species.
We analyze expression data from four species: budding  and fission yeast [4–6], human , and plants . Our primary goal is to determine sets of genes that are conserved in sequence and at the transcript level between all and subsets of these species. Our findings indicate that the set of conserved cycling genes is much larger than was previously thought. These findings are validated and explained using a large number of complementary high throughput datasets.
Results and discussion
Combined analysis of cell cycle expression data
See Materials and methods and Additional data file 3 for further details on our graph-based algorithm and on clique analysis. Also see our supporting website  for a complete list of genes identified using our algorithm.
Analysis of identified cycling genes
Our method combines expression and sequence data. This raises an obvious question; is the quality of our lists comparable to the quality of previous lists that relied on expression data alone? In other words, does our method sacrifice the accuracy with respect to the set of cycling genes in each species in order to obtain a larger set of conserved genes?
Combined, these results indicate that the species-specific lists derived using our method are comparable in quality to those of previously reported cell cycle gene lists. Additional data file 2 (Supporting Tables 1 to 3) presents the percentage overlap between the lists of cycling genes identified using our method and previously reported cycling gene lists for the four species.
Conserved cycling genes
To test the agreement of our conserved lists with complementary high-throughput datasets, we have repeated and extended our analysis discussed above but focusing only on genes included in CCC3 and CCC4. As Figures 2 and 3 and Additional data file 1 (Supporting Figure 4) show, CCC3 and CCC4 genes exhibit much stronger cell cycle characteristics when compared with the original set of cycling genes for each species. For example, in a protein-protein interaction dataset for budding yeast [18, 19], genes in CCC3 are involved in ten times more pair-wise interactions when compared with a random set of similar size from the full set of cycling genes (Figure 3c). This indicates that these genes have long been involved in the same function. Similarly, the percentages of human genes bound by two cell cycle transcription factors are much higher for the CCC3 and CCC4 sets (Nrf1 and E2f2 ; Additional data file 1 [Supporting Figure 4d]). Also, for humans the CCC3 and CCC4 sets are much more repressed in several nonproliferating tissues when compared with the full set of cycling human genes (Figure 2c). Similarly, CCC4 genes exhibit stronger cell cycle characteristics in Arabidopsis and fission yeast expression experiments (Figure 2d-e).
We have also repeated our analysis by comparing our lists with subsets of cycling genes with high amplitude in each of the four species. As shown in Additional data file 1 (Supporting Figure 5), high amplitude genes exhibit similar cell cycle characteristics to the CCC3 and CCC4 sets for human and plants. However, for the two yeasts these high amplitude genes are more similar to the full set of cycling genes. This indicates that expression analysis alone cannot be used to identify this core set of genes.
Motif analysis for budding and fission yeast genes
To further validate our findings of a large overlap between the cycling genes in the two yeast species, we turned to motif analysis. Several transcription factors are conserved between budding and fission yeast . A possible explanation for expression conservation (or lack thereof) is in the conservation (or lack of conservation) of a binding motif for these cycling genes.
We started by looking at genes bound by the budding yeast factor Swi6, which regulates transcription at the G1/S transition . We extracted three lists for this factor. The first, denoted BY6, contained cycling budding yeast genes in CCC2 determined to be bound by Swi6 . The second list, denoted FY6C, contained fission yeast genes that both were in CCC2 and had homologs in BY6. These genes were determined to be cycling and conserved by our method. The third list (FY6NC) contained noncycling fission yeast genes with cycling budding yeast homologs bound by Swi6. This latter list serves as a negative control because it contains genes that have lost their cycling status between the two species. Four motif finders were run on each dataset; SOMBRERO [24, 25], BioProspector , Consensus , and AlignACE  (see Materials and methods, below, for details). All four motif finding algorithms were able to identify the Swi6 motif in BY6 and FY6C, indicating that this motif is conserved between the two species, at least for some of the conserved cycling genes (Additional data file 1 [Supporting Figures 6 and 7]). In sharp contrast, none of these motif finders was able to identify the Swi6 motif in the upstream regions of genes in FY6NC.
Mechanistic similarities and differences between cell cycle regulation in budding and fission yeast
We have extended the motif analysis discussed above to study ten additional transcription factors that were determined to play a key role in regulating cycling genes in budding yeast [3, 16]. For each of these factors we extracted all cycling budding yeast genes determined to be bound by this factor  and their fission yeast homologs. As we did for Swi6, we further divided the fission yeast genes into two sets; the first contains fission yeast genes in CCC2 and the second (a negative control list) contains noncycling fission yeast homologs of cycling budding yeast genes. Next, we ran the four motif finders on each dataset.
Summary of motif-finding results
Budding yeast phase
Fission yeast cell cycle genes
Negative control (fission yeast non-cell-cycle genes)
Positive control (conserved budding yeast cell-cycle genes)
Extended positive control (all budding yeast CC genes)
The biologic importance of the core set of cycling genes
We have also carried out similar analyses for human genes using data from RNA interference (RNAi) experiments . In these experiments 24,373 genes were knocked down using RNAi and assessed for phenotypic influence on cell growth. For 1,152 (4.7%) of the genes, the resulting knockdown cells presented phenotypic growth defects. As Mukherji and coworkers  note in their report, roughly 6% of cycling human genes reported by Whitfield and colleagues  are included in this list. Similar to the process we conducted in yeast, we considered sequence data only and extracted from the Whitfield list those genes with homologs in the other three species. For this list, the percentage of genes increases to 10%. Again, the most enriched lists are obtained when using the CCC3 and CCC4 sets. For these, the percentage climbs to 16% (CCC3) and 17% (CCC4). These findings highlight the importance of the conserved set and support our conclusion that it contains key cycling genes.
Conserved protein complexes regulated by the cell cycle
To determine cell cycle regulated protein complexes conserved between these species, we searched for protein complexes with one or more subunits in the CCC3 set using high-throughput protein-protein interaction data. This type of data is thus far only available in budding yeast [18, 19]. Additional data file 2 (Supporting Table 12) and Additional data file 1 (Supporting Figure 18) present some of the protein complexes that we identified. Some of these complexes are known to regulate important events in the cell cycle. For example, the origin recognition complex (ORC) is a well conserved complex that is involved in the initiation of DNA synthesis . Other examples are the cohesin complex, which is responsible for binding the sister chromatids during mitosis after S phase , and the ribonucleoside-diphosphate reductase (RNR) complex, which is involved in the maintenance of the cellular pool of dNTPs .
Gene Ontology analysis of conserved cycling genes
The CCC3 list gives us our first look at the conserved core of periodically transcribed genes across evolution. Even though CCC3 contains relatively few genes (0.4% to 1.3% of the total number of genes for each species), many of these genes play a role in key processes required for growth. Using Gene Ontology (GO) analysis , we identified categories that were enriched in this set. For budding yeast these categories include cell cycle (P = 3 × 10-15), DNA replication (P = 2 × 10-13), and mitosis (P = 1 × 10-7). Similar enrichments were found for human conserved cycling genes and for fission yeast. For example, cell cycle (P = 5 × 10-17), DNA replication (P = 7 × 10-14) and cell division (P = 2 × 10-9) are enriched in humans, and cell cycle (P = 10-9) and chromatin assembly/disassembly (P = 10-9) are enriched in fission yeast. Figure 3 and Additional data file 2 (Supporting Tables 13 to 21) present P values for the various GO categories.
Some categories were more enriched in the CCC3 set than in the full list. For these categories, CCC3 contains a disproportionate number of genes when compared with the overall percentage of cycling genes. This indicates that many of the genes associated with these functions have been conserved in cyclic expression between the species. These include categories related to DNA metabolism (P = 5.7 × 10-12 for CCC3 and P = 1.1 × 10-6 for the full list) and chromatin assembly (P = 3.7 × 10-5 versus P = 0.01). In contrast, there are a number of categories that are much more enriched in the full list, indicating that they have probably evolved, or at least greatly expanded, in the individual species. These include categories such as mitosis for fission yeast (P = 1.6 × 10-4 versus P = 3.6 × 10-7) and the cell wall category, which exhibits a great deal of species-specific variation between the budding yeast, the fission yeast, and metazoans . For the human list, DNA repair and chromosome segregation were more significantly enriched in the full set (P = 5.9 × 10-4 versus P = 7.3 × 10-5, and P > 0.1 versus P = 9.0 × 10-8, respectively). Although these functions are conserved across organisms, our analysis indicates that many of these genes are cycling only in human cells, perhaps indicating that these functions have been adapted to accommodate the longer cell cycle.
Analysis of specific CCC3 genes
Partial functional knowledge is available for all but one (YPL247C) of the 72 budding yeast genes on CCC3. Sixteen of these genes encode products that are involved in DNA replication and another 23 are involved in chromosome organization and biogenesis. These include structural components (Mcms, tubulins, and histones) as well as regulatory proteins (cyclins, Cdc20, and Cin8). The mcm2 (cdc19) and mcm6 genes were previously known to be cyclic subunits of the highly conserved Mcm pre-replication complex in fission yeast [5, 36]. Our combined analysis indicates that two other genes (mcm3 and mcm5) may also be periodic, similar to the budding yeast and human Mcm subunits. Another large class of conserved cyclic genes is involved in chromosome segregation (ASE1, KIP1, NUM1, and STU2) and cytokinesis (MOB1, HOF1, KEL2, and IQG1). In addition, the list includes factors that affect transcription globally (ARP7 and TUP1) and specifically (ACE2, FKH2, and HCM1). Interestingly, the S phase specific transcription factor Hcm1 has a conserved cyclic transcript, as do 22 of its predicted targets . The fact that nearly 30% of the budding yeast CCC3 genes are potential targets of Hcm1 is consistent with the known role of Hcm1 in regulating genes involved in chromosome dynamics [3, 37].
There is only a small number of genes in CCC3 that are not obviously involved in cell cycle specific processes. These genes include three involved in metal homeostasis (SMF2, SMF3, and CTH2), some cell wall proteins (FIG2, AGA1, and SED1) and alkaline phosphatase (PHO8). These gene products could be involved in unknown aspects of the cell division cycle, or they could be evolutionarily related to other cell cycle proteins.
By applying a combined analysis, coupled with an unbiased homology metric, we were able to identify a large set of genes as conserved in sequence and cycling status between four different species: budding and fission yeast, human, and Arabidopsis.
A number of previous efforts to compare cycling gene lists derived independently for each species concluded that only a small number of genes are conserved between these species. For example, Rustici and coworkers  concluded that only 5% to 10% of cycling budding yeast genes have a cycling homolog in fission yeast. Jensen and colleagues  identified only five orthologous groups as conserved between the four species (about 1% of the cycling genes) and only eight groups (2%) between the three species of CCC3. The differences between these conclusions can be attributed to differences in the analysis of the expression and sequence data, as mentioned in the Introduction (above). We note, however, that the results presented by Oliva and coworkers  provide partial support to our conclusions. Although they did not carry out a complete conservation analysis, they found that 72 of their top 200 cycling fission yeast genes (36%) had a cycling homolog in budding yeast. Earlier work that used clustering methods to look at global expression similarities between species also supports our findings regarding the extent of expression conservation [38, 39]. Although our analysis identifies a larger fraction of conserved cycling transcripts than does that conducted by Jensen and colleagues , we find the same striking co-occurrence of cell cycle specific phosphorylation of the gene products they encode. As Additional data file 2 (Supporting Table 22) shows, when using data on Cdk1 phosphorylation  we find that 65% of tested CCC3 gene products are phosphorylated by Cdk1. This percentage is twice the percentage of phosphorylated gene products from the full set of tested cycling genes (33%) and eight times higher than the percentage of tested random genes (8%). These finding reinforces the view that there is a conserved core of genes that are regulated at multiple levels during the cell cycle in most eukaryotic cells.
Our results are strongly supported by the fact that genes conserved in two or more species display much stronger cell cycle characteristics than the full list for each species. They also show extensive interactions within the set, and almost half of the CCC4 yeast genes are essential. These observations and GO analysis indicates that these genes are crucial components of the cell cycle system. Combined, these findings support our claim that the lists we derive contain a core conserved set of cycling genes.
Our findings indicate that combined analysis of expression and sequence data leads to refined lists containing a core set of system specific genes. Although we have focused here on the cell cycle, such an analysis can be carried out to study a number of other biologic systems that have been profiled using expression experiments in multiple species, including immune response and circadian rhythm.
Materials and methods
Assigning cyclic status to genes
We applied a probabilistic graphical model to combine microarray expression data and sequence data for identification of cycling genes, as described in Lu and coworkers . We used microarray expression data reported by Spellman , Rustici , Oliva , Peng , Whitfield , and Menges  and their coworkers. We downloaded protein sequences from the National Center for Biotechnology Information website . The method starts by using gene specific expression data to compute a cycling score based on both the amplitude and periodicity . We run BLASTALL  to calculate bit scores between all pairs of sequences, as was done by Sharan and coworkers . We use a Markov random field to model the joint likelihood of the data. The (hidden) cycling status of each gene is represented by a node in the graph, and two nodes are connected by an edge if the bit score for the two genes is above a threshold. We define potential functions on nodes to capture information from the cycling scores, where we assume the scores of cycling and the noncycling genes follow a mixture of extreme value distributions, and define potential functions on edges to capture the correlation of cycling statuses between similar genes. The posterior beliefs of the cycling status of the genes are estimated using loopy belief propagation algorithm. Finally, we rank the genes by their posterior and use the name number as were used in the original papers (500 for Arabidopsis, 800 for budding yeast, 600 for fission yeast genes, and 1,000 human genes). See Additional data file 3 for complete details.
Identifying conserved sets
Genes identified as cycling in each species were used to identify conserved sets of cycling genes. This is done using the Markov clustering algorithm (MCL)  as follows. First, we start with the graph of all cycling genes. Edges in the graph are defined based on the bit score cut-off, as mentioned above. Second, for any connected subgraph in this graph, we use MCL to break it into smaller subgraphs if it has more than 30 nodes. Third, repeat the previous step until all connected subgraphs have at most 30 nodes.
Next, we assign genes to different conserved sets based on the other species represented in the subgraph to which they belong. The numbers of genes in the conserved sets are shown in Figure 3, in which the sets are organized as a tree reflecting the evolutionary relation between the four species.
For each gene in the lists, the appropriate intergenic region was extracted from the budding or fission yeast genome. Four motif finders were run on each dataset: SOMBRERO [24, 25], Consensus , BioProspector , and AlignACE . Both SOMBRERO and BioProspector require a background model, and the background was constructed from all intergenic regions (in the appropriate genome) for both cases. SOMBRERO was run using default settings, and simultaneously using all known yeast motifs as an appropriate source of prior knowledge. Consensus and BioProspector were run using default settings requiring the top ten motifs to be reported. AlignACE was run using default settings (using a seed motif length of 10), and provided with the background intergenic GC content (31.45% for fission and 35.3% for budding). See Additional data file 3 for further details.
GO enrichment P values were computed using STEM , which relies on hyper-geometric distribution. Corrected P values were computed by permutation analysis using STEM.
Additional data files
This work was partially supported by NSF CAREER award 0448453 to ZBJ, by NSF Grant 0225656, and by a tobacco settlement grant from the Pennsylvania Department of Health.
- Whitfield ML, Sherlock G, Saldanha AJ, Murray JI, Ball CA, Alexander KE, Matese JC, Perou CM, Hurt MM, Brown PO, et al: Identification of genes periodically expressed in the human cell cycle and their expression in tumors. Mol Biol Cell. 2002, 13: 1977-2000. 10.1091/mbc.02-02-0030..PubMedPubMed CentralView ArticleGoogle Scholar
- Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, Brown PO, Botstein D, Futcher B: Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell. 1998, 9: 3273-3297.PubMedPubMed CentralView ArticleGoogle Scholar
- Pramila T, Wu W, Miles S, Noble WS, Breeden LL: The forkhead transcription factor Hcm1 regulates chromosome segregation genes and fills the S-phase gap in the transcriptional circuitry of the cell cycle. Genes Dev. 2006, 20: 2266-2278. 10.1101/gad.1450606.PubMedPubMed CentralView ArticleGoogle Scholar
- Rustici G, Mata J, Kivinen K, Lio P, Penkett CJ, Burns G, Hayles J, Brazma A, Nurse P, Bahler J: Periodic gene expression program of the fission yeast cell cycle. Nat Genet. 2004, 36: 809-817. 10.1038/ng1377.PubMedView ArticleGoogle Scholar
- Oliva A, Rosebrock A, Ferrezuelo F, Pyne S, Chen H, Skiena S, Futcher B, Leatherwood J: The cell cycle-regulated genes of Schizosaccharomyces pombe. PLoS Biol. 2005, 3: e225-10.1371/journal.pbio.0030225.PubMedPubMed CentralView ArticleGoogle Scholar
- Peng X, Karuturi RKM, Miller LD, Lin K, Jia Y, Kondu P, Wang L, Wong LS, Liu ET, Balasubramanian MK, et al: Identification of cell cycle-regulated genes in fission yeast. Mol Biol Cell. 2005, 16: 1026-1042. 10.1091/mbc.E04-04-0299.PubMedPubMed CentralView ArticleGoogle Scholar
- Menges M, Hennig L, Gruissem W, Murray JAH: Cell cycle-regulated gene expression in Arabidopsis. J Biol Chem. 2002, 277: 41987-42002. 10.1074/jbc.M207570200.PubMedView ArticleGoogle Scholar
- Laub MT, McAdams HH, Feldblyum T, Fraser CM, Shapiro L: Global analysis of the genetic network controlling a bacterial cell cycle. Science. 2000, 290: 2144-2148. 10.1126/science.290.5499.2144.PubMedView ArticleGoogle Scholar
- Dyczkowski J, Vingron M: Comparative analysis of cell cycle regulated genes in eukaryotes. Genome Inform. 2005, 16: 125-131.PubMedGoogle Scholar
- Jensen LJ, Jensen TS, de Lichtenberg U, Brunak S, Bork P: Co-evolution of transcriptional and post-translational cell-cycle regulation. Nature. 2006, 443: 594-597.PubMedGoogle Scholar
- Simon I, Siegfried Z, Ernst J, Bar-Joseph Z: Combining static and time series data to determine the quality of expression profiles in time series experiments. Nat Biotechnol. 2005, 23: 1503-1508. 10.1038/nbt1164.PubMedView ArticleGoogle Scholar
- Lu Y, Rosenfeld R, Bar-Joseph Z: Identifying cycling genes by combining sequence homology and expression data. Bioinformatics. 2006, 22: e314-e322. 10.1093/bioinformatics/btl229.PubMedView ArticleGoogle Scholar
- De Lichtenberg U, Jensen LJ, Fausboll A, Jensen TS, Bork P, Brunak S: Comparison of computational methods for the identification of cell cycle-regulated genes. Bioinformatics. 2005, 21: 1164-1171. 10.1093/bioinformatics/bti093.PubMedView ArticleGoogle Scholar
- Enright AJ, Van Dongen S, Ouzounis CA: An efficient algorithm for large-scale detection of protein families. Nucleic Acids Res. 2002, 30: 1575-1584. 10.1093/nar/30.7.1575.PubMedPubMed CentralView ArticleGoogle Scholar
- Supporting Website at Systems Biology Group. [http://www.sb.cs.cmu.edu/CCC/]
- Simon I, Barnett J, Hannett N, Harbison CT, Rinaldi NJ, Volkert TL, Wyrick JJ, Zeitlinger J, Gifford DK, Jaakkola TS, et al: Serial regulation of transcriptional regulators in the yeast cell cycle. Cell. 2001, 106: 697-708. 10.1016/S0092-8674(01)00494-9.PubMedView ArticleGoogle Scholar
- Penkett CJ, Morris JA, Wood V, Bähler J: YOGY: a web-based, integrated database to retrieve protein orthologs and associated Gene Ontology terms. Nucleic Acids Res. 2006, 34: W330-W334. 10.1093/nar/gkl311.PubMedPubMed CentralView ArticleGoogle Scholar
- Krogan NJ, Cagney G, Yu H, Zhong G, Guo X, Ignatchenko A, Li J, Pu S, Datta N, Tikuisis AP, et al: Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature. 2006, 440: 637-643. 10.1038/nature04670.PubMedView ArticleGoogle Scholar
- Gavin AC, Aloy P, Grandi P, Krause R, Boesche M, Marzioch M, Rau C, Jensen LJ, Bastuck S, Dumpelfeld B, et al: Proteome survey reveals modularity of the yeast cell machinery. Nature. 2006, 440: 631-636. 10.1038/nature04532.PubMedView ArticleGoogle Scholar
- Ren B, Cam H, Takahashi Y, Volkert T, Terragni J, Young RA, Dynlacht BD: E2F integrate cell cycle progression with DNA repair, replication, and G2/M checkpoints. Gene Dev. 2002, 16: 245-256. 10.1101/gad.949802.PubMedPubMed CentralView ArticleGoogle Scholar
- Bähler J: Cell-cycle control of gene expression in budding and fission yeast. Annu Rev Genet. 2005, 39: 69-94. 10.1146/annurev.genet.39.110304.095808.PubMedView ArticleGoogle Scholar
- Breeden L, Nasmyth K: Cell cycle control of the yeast HO gene: Cis- and Trans-acting regulators. Cell. 1987, 48: 389-397. 10.1016/0092-8674(87)90190-5.PubMedView ArticleGoogle Scholar
- Harbison CT, Gordon DB, Lee TI, Rinaldi NJ, Macisaac KD, Danford TW, Hannett NM, Tagne JB, Reynolds DB, Yoo J, et al: Transcriptional regulatory code of a eukaryotic genome. Nature. 2004, 431: 99-104. 10.1038/nature02800.PubMedPubMed CentralView ArticleGoogle Scholar
- Mahony S, Hendrix D, Golden A, Smith TJ, Rokhsar DS: Transcription factor binding site identification using the self-organizing map. Bioinformatics. 2005, 21: 1807-1814. 10.1093/bioinformatics/bti256.PubMedView ArticleGoogle Scholar
- Mahony S, Golden A, Smith TJ, Benos PV: Improved detection of DNA motifs using a self-organized clustering of familial binding profiles. Bioinformatics. 2005, i283-i291. 10.1093/bioinformatics/bti1025. Suppl 1Google Scholar
- Liu X, Brutlag DL, Liu JS: BioProspector: discovering conserved DNA motifs in upstream regulatory regions of co-expressed genes. Pac Symp Biocomput. 2001, 6: 127-138.Google Scholar
- Hertz GZ, Hartzell GW, Stormo GD: Identification of consensus patterns in unaligned DNA sequences known to be functionally related. Comput Appl Biosci. 1990, 6: 81-92.PubMedGoogle Scholar
- Roth FP, Hughes JD, Estep PW, Church GM: Finding DNA regulatory motifs within unaligned noncoding sequences clustered by whole-genome mRNA quantitation. Nat Biotechnol. 1998, 16: 939-945. 10.1038/nbt1098-939.PubMedView ArticleGoogle Scholar
- Winzeler EA, Shoemaker DD, Astromoff A, Liang H, Anderson K, Andre B, Bangham R, Benito R, Boeke JD, Bussey H, et al: Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science. 1999, 285: 901-906. 10.1126/science.285.5429.901.PubMedView ArticleGoogle Scholar
- Mukherji M, Bell R, Supekova L, Wang Y, Orth AP, Batalov S, Miraglia L, Huesken D, Lange J, Martin C, et al: Genome-wide functional analysis of human cell-cycle regulators. Proc Natl Acad Sci USA. 2006, 103: 14819-14824. 10.1073/pnas.0604320103.PubMedPubMed CentralView ArticleGoogle Scholar
- Micklem G, Rowley A, Harwood J, Nasmyth K, Diffley JFX: Yeast origin recognition complex is involved in DNA replication and transcriptional silencing. Nature. 1993, 366: 87-89. 10.1038/366087a0.PubMedView ArticleGoogle Scholar
- Nasmyth K: Disseminating the genome: joining, resolving, and separating sister chromatids during mitosis and meiosis. Annu Rev Genet. 2001, 35: 673-745. 10.1146/annurev.genet.35.102401.091334.PubMedView ArticleGoogle Scholar
- Xu H, Faber C, Uchiki T, Racca J, Dealwis C: Structures of eukaryotic ribonucleotide reductase I define gemcitabine diphosphate binding and subunit assembly. Proc Natl Acad Sci USA. 2006, 103: 4028-4033. 10.1073/pnas.0600440103.PubMedPubMed CentralView ArticleGoogle Scholar
- The Gene Ontology Consortium: Gene Ontology: tool for the unification of biology. Nat Genet. 2000, 25: 25-29. 10.1038/75556.PubMed CentralView ArticleGoogle Scholar
- Balasubramanian MK, Bi E, Glotzer M: Comparative analysis of cytokinesis in budding yeast, fission yeast and animal cells. Curr Biol. 2004, 14: 806-818. 10.1016/j.cub.2004.09.022.View ArticleGoogle Scholar
- Anderson M, Ng SS, Marchesi V, MacIver FH, Stevens FE, Riddell T, Glover DM, Hagan IM, McInerny CJ: Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. EMBO J. 2002, 21: 5745-5755. 10.1093/emboj/cdf564.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhu G, Davis TN: The fork head transcription factor Hcm1p participates in the regulation of SPC110, which encodes the calmodulin-binding protein in the yeast spindle pole body. Biochem Biophys Acta. 1998, 1448: 236-244. 10.1016/S0167-4889(98)00135-9.PubMedView ArticleGoogle Scholar
- Stuart JM, Segal E, Koller D, Kim SK: A gene-coexpression network for global discovery of conserved genetic modules. Science. 2003, 302: 249-255. 10.1126/science.1087447.PubMedView ArticleGoogle Scholar
- Bergmann S, Ihmels J, Barkai N: Similarities and differences in genome-wide expression data of six organisms. PLoS Biol. 2004, 2: e9-10.1371/journal.pbio.0020009.PubMedPubMed CentralView ArticleGoogle Scholar
- Ubersax JA, Woodbury EL, Quang PN, Paraz M, Blethrow JD, Shah K, Shokat KM, Morgan DO: Targets of the cyclin-dependent kinase Cdk 1. Nature. 2003, 425: 859-864. 10.1038/nature02062.PubMedView ArticleGoogle Scholar
- National Center for Biotechnology Information. [http://www.ncbi.nlm.nih.gov/]
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410.PubMedView ArticleGoogle Scholar
- Sharan R, Suthram S, Kelley RM, Kuhn T, McCuine S, Uetz P, Sittler T, Karp RM, Ideker T: Conserved patterns of protein interaction in multiple species. Proc Natl Acad Sci USA. 2005, 102: 1974-1979. 10.1073/pnas.0409522102.PubMedPubMed CentralView ArticleGoogle Scholar
- Ernst J, Bar-Joseph Z: STEM: a tool for the analysis of short time series gene expression data. BMC Bioinformatics. 2006, 7: 191-10.1186/1471-2105-7-191.PubMedPubMed CentralView ArticleGoogle Scholar
- Mondesert O, McGowan CH, Russell P: Cig2, a B-type cyclin, promotes the onset of S in S. pombe. Mol Cell Biol. 1996, 16: 1527-1523.PubMedPubMed CentralView ArticleGoogle Scholar
- Pagano M, Pepperkok R, Verde F, Ansorge W, Draetta G: Cyclin A is required at two points in the human cell cycle. EMBO J. 1992, 11: 961-971.PubMedPubMed CentralGoogle Scholar
- Lee DG, Bell SP: Architecture of the yeast origin recognition complex bound to origins of DNA replication. Mol Cell Biol. 1997, 17: 7159-7168.PubMedPubMed CentralView ArticleGoogle Scholar
- Garcia MA, Vardy L, Koonrugsa N, Toda T: Fission yeast ch-TOG/XMAP 215 homologueAlp 14 connectsmitotic spindles with the kinetochore and is a component of the Mad 2-dependent spindle checkpoint. EMBO J. 2001, 20: 3389-3401. 10.1093/emboj/20.13.3389.PubMedPubMed CentralView ArticleGoogle Scholar
- Javerzat JP, Cranston G, Allshire RR: Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed. Nucleic Acids Res. 1996, 24: 4676-4683. 10.1093/nar/24.23.4676.PubMedPubMed CentralView ArticleGoogle Scholar
- Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, Storz G, Botstein D, Brown PO: Genomic expression programs in the response of yeast cells to environmental changes. Mol Biol Cell. 2000, 11: 4241-4257.PubMedPubMed CentralView ArticleGoogle Scholar
- Shyamsundar R, Kim YH, Higgins JP, Montgomery K, Jorden M, Sethuraman A, van de Rijn M, Botstein D, Brown PO, Pollack JR: A DNA microarray survey of gene expression in normal human tissues. Genome Biol. 2005, 6: R22-10.1186/gb-2005-6-3-r22.PubMedPubMed CentralView ArticleGoogle Scholar
- Nagpal P, Ellis CM, Weber H, Ploense SE, Barkawi LS, Guilfoyle TJ, Hagen G, Alonso JM, Cohen JD, Farmer EE, et al: Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation. Development. 2005, 132: 4107-4118. 10.1242/dev.01955.PubMedView ArticleGoogle Scholar
- Mata J, Bähler J: Global roles of Ste11p, cell type, and pheromone in the control of gene expression during early sexual differentiation in fission yeast. Proc Natl Acad Sci USA. 2006, 103: 15517-15522. 10.1073/pnas.0603403103.PubMedPubMed CentralView ArticleGoogle Scholar
- Gergely F, Draviam VM, Raff JW: The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells. Gene Dev. 2003, 17: 336-341. 10.1101/gad.245603.PubMedPubMed CentralView ArticleGoogle Scholar
- Usui T, Maekawa H, Pereira G, Schiebel E: The XMAP 215 homologue Stu 2 at yeast spindle pole bodies regulates microtubule dynamics and anchorage. EMBO J. 2003, 22: 4779-4793. 10.1093/emboj/cdg459.PubMedPubMed CentralView ArticleGoogle Scholar
- Stoler S, Keith K, Curnick K, Fitzgerald-Hayes M: A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis. Gene Dev. 1995, 9: 573-586. 10.1101/gad.9.5.573.PubMedView ArticleGoogle Scholar
- Takahashi K, Chen E, Yanagida M: Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast. Science. 2000, 288: 2215-2219. 10.1126/science.288.5474.2215.PubMedView ArticleGoogle Scholar
- Troxell C, Sweezy M, West R, Reed K, Carson B, Pidoux A, Cande W, McIntosh J: pkl1+ and klp2+: two kinesins of the Kar3 subfamily in fission yeast perform different functions in both mitosis and meiosis. Mol Biol Cell. 2001, 12: 3476-3488.PubMedPubMed CentralView ArticleGoogle Scholar
- Mayer M, Pot I, Chang M, Xu H, Aneliunas V, Kwok T, Newitt R, Aebersold R, Boone C, Brown G, et al: Identification of protein complexes required for efficient sister chromatid cohesion. Mol Biol Cell. 2004, 15: 1736-1745. 10.1091/mbc.E03-08-0619.PubMedPubMed CentralView ArticleGoogle Scholar
- Gruneberg U, Neef R, Honda R, Nigg EA, Barr FA: Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2. J Cell Biol. 2004, 166: 167-172. 10.1083/jcb.200403084.PubMedPubMed CentralView ArticleGoogle Scholar
- Provenzani A, Fronza R, Loreni F, Pascale A, Amadio M, Quattrone A: Global alterations in mRNA polysomal recruitment in a cell model of colorectal cancer progression to metastasis. Carcinogenesis. 2006, 27: 1323-1333. 10.1093/carcin/bgi377.PubMedView ArticleGoogle Scholar
- Micklem G, Rowley A, Harwood J, Nasmyth K, Diffley JFX: Yeast origin recognition complex is involved in DNA replication and transcriptional silencing. Nature. 1993, 366: 87-89. 10.1038/366087a0.PubMedView ArticleGoogle Scholar
- Remm M, Storm C, Sonnhammer E: Automatic clustering of orthologs and in-paralogs from pairwise species comparisons. J Mol Biol. 2001, 314: 1041-1052. 10.1006/jmbi.2000.5197.PubMedView ArticleGoogle Scholar
- MacIsaac KD, Wang T, Gordon B, Gifford DK, Stormo GD, Fraenkel E: An improved map of conserved regulatory sites for Saccharomyces cerevisiae. BMC Bioinformatics. 2006, 7: 113-10.1186/1471-2105-7-113.PubMedPubMed CentralView ArticleGoogle Scholar
- Rual J, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz G, Gibbons F, Dreze M, Ayivi-Guedehoussou N, et al: Towards a proteome-scale map of the human protein-protein interaction network. Nature. 2005, 437: 1173-1178. 10.1038/nature04209.PubMedView ArticleGoogle Scholar
- Saccharomyces Genome Deletion Project website. [http://www-sequence.stanford.edu/group/yeast_deletion_project/deletions3.html]
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.