Consistent dissection of the protein interaction network by combining global and local metrics

A new network decomposition method is proposed that uses both a global metric and a local metric to identify protein interaction modules in the protein interaction network.


YLL006W
[MMM1] Mitochondrial outer membrane protein required for normal mitochondrial morphology and mtDNA stability; involved in tethering mitochondria to the actin cytoskeleton and in anchoring mtDNA nucleoids mitochondrial outer membrane protein Null mutant is viable, fails to grow on nonfermentable carbon sources, demonstrates abnormal mitochondrial morphology, fails to segregate mitochondria into daughter cells [CAT5] Mitochondrial inner membrane protein directly involved in ubiquinone biosynthesis, essential for several other metabolic pathways including respiration and gluconeogenic gene activation may encode a protein involved in one or more monoxygenase or hydroxylase steps of ubiquinone biosynthesis Null mutant is viable, results in complete loss of glucose derepression affecting gluconeogenic key enzymes.
Respiration, but not mitochondrial cytochrome c oxidase activity, are also affected; fails to synthesize ubiquinone [ERG28] Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Null mutant is viable; random budding in diploid null mutants; null cells have an unusual sterol content.

YHR072W
[ERG7] Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis 2,3oxidosqualene-lanosterol cyclase [NMA1] Nicotinic acid mononucleotide adenylyltransferase, involved in NAD(+) salvage pathway nicotinamide/nicotinic acid mononucleotide adenylyltransferase Null: viable. Other phenotypes: 2 or more copies increase rDNA and telomeric silencing YGR010W [NMA2] Nicotinic acid mononucleotide adenylyltransferase, involved in NAD(+) salvage pathway nicotinamide/nicotinic acid mononucleotide adenylyltransferase Null: viable. Other phenotypes: 2 or more copies increase rDNA and telomeric silencing YLR438W [CAR2] L-ornithine transaminase (OTAse), catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive ornithine aminotransferase Catabolism of arginine defective 0012 GO_TERM:[bud site selection] P-Value:1.1e-05 YOR301W [RAX1] Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulinrelated peptide superfamily YGR041W [BUD9] Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole In null mutants bipolar-budding cells bud preferentially at distal pole YLR353W [BUD8] Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole A bud8 bud9 double mutant buds almost exclusively from the proximal pole 0013 GO_TERM:[meiotic DNA double-strand break formation] P-Value:3.5e-08 YLR329W [REC102] Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination 23 kDa protein containing a putative leucine zipper|meiosis specific recombination protein Reduced meiotic recombination; inviable spores; mutant is rescued by spo13 and is epistatic to rad52 YHL022C [SPO11] Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation early meiosis-specific recombination protein YHR157W [REC104] Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination meiosis-specific protein Null mutant is viable, rec104 mutants exhibit reduced meiotic DNA recombination, executes meiosis I early; rec104 is rescued by spo13 and is epistatic to rad52 spo13 [OCA2] Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts YNL032W [SIW14] Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm tyrosine phosphatase Null mutant fails to show cell cycle arrest upon nutrient starvation, is sensitive to 5mM caffeine and 1M NaCL, and shows delocalized actin upon nutrient starvation; synthetically lethal with whi2, on minimal medium only YNL099C [OCA1] Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA YCR095C [YCR095C] Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts YHL029C [YHL029C] Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts 0022 GO_TERM:[translation regulator activity] P-Value:2.6e-11 YER153C [PET122] Specific translational activator for the COX3 mRNA that acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane translational activator of cytochrome C oxidase subunit III petite; unable to grow on non-fermentable carbon sources YLR067C [PET309] Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; located in the mitochondrial inner membrane petite; unable to grow on non-fermentable carbon sources YGR222W [PET54] Protein required for splicing of the COX1 intron AI5 beta; also specifically required, together with Pet122p and Pet494p, for translation of the COX3 mRNA; located in the mitochondrial inner membrane petite; unable to grow on non-fermentable carbon sources YMR257C [PET111] Specific translational activator for the COX2 mRNA, located in the mitochondrial inner membrane translational activator of cytochrome C oxidase subunit II YNR045W [PET494] Specific translational activator for the COX3 mRNA that acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane translational activator of cytochrome C oxidase petite; unable to grow on non-fermentable carbon sources 0023 GO_TERM:[pyridoxine metabolism] P-Value:6.9e-22

YFL060C
[SNO3] Protein of unknown function, nearly identical to Sno2p; expression is induced before the diauxic shift and also in the absence of thiamin YNL334C [SNO2] Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin YMR095C [SNO1] Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Null mutant is viable, sensitive to 6-azauracil and methylene blue.

YMR322C
[SNO4] Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp32p, and Hsp33p; member of the DJ-1/ThiJ/PfpI superfamily; may have a role in pyridoxine metabolism YNL333W [SNZ2] Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p hypersporulation YFL059W [SNZ3] Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO3 hypersporulation YMR096W [SNZ1] Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins highly conserved 35 kDa protein that shows increased expression after entry into stationary phase Null mutant is viable, sensitive to 6-azauracil and methylene blue. [RMD5] Cytosolic protein required for sporulation; also required for the ubiquitination of the gluconeogenetic enzyme fructose-1,6bisphosphatase, which is degraded rapidly after the switch from gluconeogenesis to glycolysis YBR105C [VID24] Peripheral membrane protein located at Vid (vacuole import and degradation) vesicles; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; involved in proteasome-dependent catabolite degradation of FBPase peripheral vesicle membrane protein Null mutant is viable, defective in fructose-1,6-bisphosphatase dergadation YIL017C [VID28] Protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm YIL097W [FYV10] Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains CTLH domain Null mutant is viable but exhibits K1 killer toxin hypersensitivity.

YBL049W
[MOH1] Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for viability in stationary phase YCL039W [GID7] Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions YGL227W [VID30] Protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); shifts the balance of nitrogen metabolism toward the production of glutamate; localized to the nucleus and the cytoplasm Null mutant is viable but exhibits vacuolar degradation of cytosolic proteins; mutants are also sensitive to starvation.

YMR135C
[GID8] Protein of unknown function, involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; contains LisH and CTLH domains, like Vid30p; dosage-dependent regulator of START 0026 GO_TERM:[signal recognition particle (sensu Eukaryota)] P-Value:4.6e-17 OVERLAP:[Signal recognition particle (SRP)] <520.40> SIZE:6 YGR250C YER155C [BEM2] Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence rho GTPase activating protein (GAP) randomized bud-site selection at 26 degrees C and defective bud emergence and growth at 37 degrees C YDL051W [LHP1] RNA binding protein required for maturation of tRNA and snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen

YML105C
[SEC65] Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 YKL122C [SRP21] Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm signal recognition particle component Null mutant is viable, associated with slow cell growth and inefficient protein translocation across the ER membrane

YPL210C
[SRP72] Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane signal recognition particle component Null mutant is viable, associated with slow cell growth and inefficient protein translocation across the ER membrane

YPR088C
[SRP54] Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain

YDL092W
[SRP14] Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14

YPL243W
[SRP68] Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane signal recognition particle component Null mutant is viable, associated with slow cell growth and inefficient protein translocation across the ER membrane 0027 YAL036C [RBG1] Member of the DRG family of GTP-binding proteins; interacts with translating ribosomes and with Tma46p

YLR284C
[ECI1] Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced d3,d2-Enoyl-CoA Isomerase Null mutant is viable but fails to metabolize unsaturated fatty acids YOR180C [DCI1] Peroxisomal delta(3,5)-delta(2,4)-dienoyl-CoA isomerase, involved in fatty acid metabolism, contains peroxisome targeting signals at amino and carboxy termini delta(3,5)-delta(2,4)-dienoyl-CoA isomerase 0029 GO_TERM:[peroxisome] P-Value:5.1e-08 YGR239C [PEX21] Part of a two-member peroxin family (Pex18p and Pex21p) specifically required for peroxisomal targeting of the Pex7p peroxisomal signal recognition factor and PTS2-type peroxisomal matrix proteins peroxin YDR142C [PEX7] Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) beta-transducin-related (WD-40) protein family Mutant is defective in assembling specific proteins into peroxisomes (assembles catalase and acyl-CoA oxidase but not thiolase) and cannot utilize oleic acid YHR160C [PEX18] Part of a two-member peroxin family (Pex18p and Pex21p) specifically required for peroxisomal targeting of the Pex7p peroxisomal signal recognition factor and PTS2 peroxisomal matrix proteins peroxin Null mutant is viable but has reduced growth on oleate, partial impairment of peroxisome biogenesis YIL160C [POT1] 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during betaoxidation of fatty acids 3-oxoacyl CoA thiolase Null mutant is viable, unable to use oleic acid as a carbon source 0030 GO_TERM:[peroxisome organization and biogenesis] P-Value:5.9e-32 YAL055W [PEX22] Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Null mutant is viable and oleate minus YGR133W [PEX4] Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis ubiquitinconjugating protein family YOL147C [PEX11] Peroxisomal membrane protein required for peroxisome proliferation and medium-chain fatty acid oxidation, most abundant protein in the peroxisomal membrane, regulated by Adr1p and Pip2p-Oaf1p, promoter contains ORE and UAS1-like elements peroxin|peroxisomal membrane protein YDR329C [PEX3] Peroxisomal membrane protein (PMP) required to recruit Pex19p chaperone to peroxisomes; plays selective, essential, direct role in PMP import as a docking factor for Pex19p 48 kDa peroxisomal integral membrane protein Mutant lacks peroxisomes and shows cytosolic mislocalization of peroxisomal matrix enzymes YOL044W [PEX15] Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly 44 kDa phosphorylated integral peroxisomal membrane protein YDL065C [PEX19] Chaperone and import receptor for newly-synthesized class I peroxisomal membrane proteins (PMPs), binds PMPs in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane 40 kDa farnesylated protein associated with peroxisomes mutant lacks morphologically recognizable peroxisomes and shows mislocalization of peroxisomal matrix proteins YDR265W [PEX10] RING finger peroxisomal membrane peroxin required for peroxisomal matrix protein import, interacts with Pex12p, links ubiquitinconjugating Pex4p to protein import machinery; mutations in human homolog cause a variety of peroxisomal disorders C3HC4 zinc-binding integral peroxisomal membrane protein|peroxin mutant lacks morphologically recognizable peroxisomes and shows cytosolic mislocalization of peroxisomal matrix proteins YGR077C [PEX8] Intraperoxisomal organizer of the peroxisomal import machinery, tightly associated with the lumenal face of the peroxisomal membrane, essential for peroxisome biogenesis, binds PTS1-signal receptor Pex5p peroxisome associated protein containing a PTS1 signal YJL210W [PEX2] RING-finger peroxin, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms putative translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import CH3HC4 zinc-binding integral peroxisomal membrane protein|peroxin Null mutant is viable but lacks morphologically recognizable peroxisomes and shows cytosolic mislocalization of peroxisomal matrix proteins YMR026C [PEX12] RING-finger peroxisomal membrane peroxin that plays an essential role in peroxisome biogenesis and peroxisomal matrix protein import, forms translocation subcomplex with Pex2p and Pex10p C3HC4 zinc-binding integral peroxisomal membrane protein mutant lacks morphologically recognizable peroxisomes and shows cytosolic mislocalization of peroxisomal matrix proteins YLR191W [PEX13] Integral peroxisomal membrane receptor for the PTS1 peroxisomal matrix protein signal recognition factor Pex5p, required for the translocation of peroxisomal matrix proteins, also interacts with Pex7p and Pex14p, contains a C-terminal SH3 domain contains Src homology 3 (SH3) domain Defective in peroxisome assembly YNL214W [PEX17] Peroxisomal membrane protein component of the peroxisomal translocation machinery, required for peroxisome biogenesis, binds Pex14p peroxin mutant lacks morphologically recognizable peroxisomes and shows mislocalization of peroxisomal matrix proteins YDR244W [PEX5] Peroxisomal membrane signal receptor for C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import, tetratricopeptide repeat protein, also involved in PTS1-independent import 69 kDa protein containing tetratricopeptide repeat (TPR)|peroxin Null mutant is viable but accumulates peroxisomal, leaflet-like membrane structures and exhibits deficient import of some peroxisomal matrix enzymes, particularly proteins with an SKL-like import signal YGL153W [PEX14] Peroxisomal membrane protein that is a central component of the peroxisomal protein import machinery, interacts with PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p peroxin Null mutant is viable but is unable to grow on oleate and lacks peroxisomes 0031 GO_TERM:[ubiquinol-cytochrome-c reductase activity] P-Value:2.5e-04 OVERLAP:[Cytochrome bc1 complex (Ubiquinol-cytochrome c reductase complex, complex III)] <420.30> SIZE:10 YBR018C [GAL7] Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-Dgalactose-1-phosphate in the second step of galactose catabolism galactose-1-phosphate uridyl transferase Null mutant is viable and cannot utilize galactose.

YPR054W
[SMK1] Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for spore morphogenesis MAP kinase smk1 asci are defective in organizing spore wall assembly and display enhanced sensitivity to enzymatic digestion, heat shock, and ether YNL177C [MRPL22] Mitochondrial ribosomal protein of the large subunit YMR036C [MIH1] Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 protein phosphatase Null mutant is viable <br> Short G2 delay

YBL045C
[COR1] Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain coenzyme QH2 cytochrome c reductase 44 kDa core protein subunit deficiency in cytochrome b; slow growth on glycerol

YPR191W
[QCR2] Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme 40 kDa ubiquinol cytochrome-c reductase core protein 2 Null mutant is viable and grows slowly on glycerol [TYS1] Cytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNA synthetase that aminoacylates tRNA(Tyr), required for cytoplasmic protein synthesis, interacts with positions 34 and 35 of the anticodon of tRNATyr tyrosine-tRNA ligase

YMR158W
[MRPS8] Mitochondrial ribosomal protein of the small subunit

Q0140
[VAR1] Mitochondrial ribosomal protein of the small subunit, mitochondrially-encoded; polymorphic in different strains due to variation in number of AAT (asparagine) codons; translated near the mitochondrial inner membrane mitochondrial ribosomal protein

YPR166C
[MRP2] Mitochondrial ribosomal protein of the small subunit 14 kDa mitochondrial ribosomal protein|similar to E. coli S14 protein defective mitochondrial protein synthesis; absence of a and b type cytochromes; reduced levels of mitochondrial 15 S rRNA; defective processing of apocytochrome b intron; convert to rho-and rho0 at high frequency

YDL045W-A
[MRP10] Mitochondrial ribosomal protein of the small subunit mitochondrial ribosome 37 S subunit component Null mutant is viable, defective in mitochondrial translation and shows a tendency to accumulate deletions in mitochondrial DNA

YDR494W
[RSM28] Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Mitochondrial ribosomal small subunit protein Null: Viable, Pet+, mild H2O2 sensitivity. Other phenotypes: Dominant suppressor allele, due to internal deletion, selected by asking for increased expression of COX2 alleles with short deletions in the leader peptide coding region.

YNR037C
[RSM19] Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein mitochondrial ribosome small subunit component YKL003C [MRP17] Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3specific translational activator ribosomal protein MRP17 petite

YBL090W
[MRP21] Mitochondrial ribosomal protein of the large subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences mitochondrial ribosome small subunit component Null mutant is viable, exhibits completely blocked mitochondrial gene expression; missense mutations suppress 5'-UTL mutations in at least 2 mitochondrial mRNAs

YMR188C
[MRPS17] Mitochondrial ribosomal protein of the small subunit YDR124W YJL063C [MRPL8] Mitochondrial ribosomal protein of the large subunit Null mutant is viable; shows loss of mitochondrial function, instability of mitochondrial DNA YNR036C

YGR215W
[RSM27] Mitochondrial ribosomal protein of the small subunit mitochondrial ribosome small subunit component

YNL306W
[MRPS18] Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins

YGR084C
[MRP13] Mitochondrial ribosomal protein of the small subunit 35 kDa mitochondrial ribosomal small subunit protein Null mutant is viable, no impairment in ribosome synthesis or function YOL027C [MDM38] Mitochondrial inner membrane protein, required for K+/H+ exchange and for normal mitochondrial morphology and inheritance; associates with mitochondrial ribosomes; human ortholog Letm1 is implicated in Wolf-Hirschhorn syndrome

YJR101W
[RSM26] Mitochondrial ribosomal protein of the small subunit mitochondrial ribosome small subunit component YPR125W [YLH47] Mitochondrial inner membrane protein exposed to the mitochondrial matrix, associates with mitochondrial ribosomes, NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome

YBR146W
[MRPS9] Mitochondrial ribosomal protein of the small subunit ribosomal protein S9 (putative) Null mutant is viable, respiration deficient, exhibits defects in mitochondrial protein synthesis as indicated by a loss of cytochrome c oxidase activity

YPL118W
[MRP51] Mitochondrial ribosomal protein of the large subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences mitochondrial ribosome small subunit component Null mutant is viable, exhibits completely blocked mitochondrial gene expression; missense mutations suppress 5'-UTL mutations in at least 2 mitochondrial mRNAs

YIL093C
[RSM25] Mitochondrial ribosomal protein of the small subunit mitochondrial ribosome small subunit component Null mutant is viable, but unable to respire. YOR158W [PET123] Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator mitochondrial ribosomal protein of small subunit Null mutant is viable but is rho-(with large deletions in mtDNA); pet123 mutations can suppress pet122 mutations; some pet123 alleles show synthetic phenotypes with mrp1 mutations YIL070C [MAM33] Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R YDR036C [EHD3] Protein of unconfirmed function, plays an indirect role in endocytic membrane trafficking, member of a family of enoyl-CoA hydratase/isomerases YJR113C [RSM7] Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein mitochondrial ribosome small subunit component YHL004W [MRP4] Mitochondrial ribosomal protein of the small subunit mitochondrial ribosomal protein|mitochondrial ribosome 37 S subunit component|similar to E. coli ribosomal protein S2

YDR175C
[RSM24] Mitochondrial ribosomal protein of the small subunit mitochondrial ribosome small subunit component

YGR165W
[MRPS35] Mitochondrial ribosomal protein of the small subunit

YDR347W
[MRP1] Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein 37 kDa mitochondrial ribosomal protein defective mitochondrial protein synthesis; absence of a and b type cytochromes; reduced levels of mitochondrial 15 S rRNA; defective processing of apocytochrome b intron; convert to rho-and rho0 at high frequency

YPL013C
[MRPS16] Mitochondrial ribosomal protein of the small subunit

YDR337W
[MRPS28] Mitochondrial ribosomal protein of the small subunit ribosomal protein (E. coli S15) Null mutant is viable, unable to respire, spontaneously loses portions of its mitochondrial genomes at a high frequencY

YER050C
[RSM18] Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein mitochondrial ribosome small subunit component null is unable to grow on glycerol

YKL155C
[RSM22] Mitochondrial ribosomal protein of the small subunit mitochondrial ribosome small subunit component Null mutant is viable, unable to respire YDR041W [RSM10] Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins mitochondrial ribosome small subunit component

YGL129C
[RSM23] Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase [GAP1] General amino acid permease; localization to the plasma membrane is regulated by nitrogen source general amino acid permease abolished activity of the general amino acid transport system YKR007W [MEH1] Component of the EGO complex, which is involved in the regulation of microautophagy; localizes to the vacuolar membrane, loss results in a defect in vacuolar acidification YML121W [GTR1] Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle, through its homolog and binding partner, Gtr2p; involved in phosphate transport and invasive growth; human RagA and RagB proteins are functional homologs small GTPase (putative) Null mutant is viable but grows slowly, is cold-sensitive, and has defects in phosphate uptake

YBR077C
[SLM4] Component of the EGO complex, which is involved in the regulation of microautophagy; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase YGR163W [GTR2] Cytoplasmic GTP binding protein, negative regulator of the Ran/Tc4 GTPase cycle downstream of Gtr1p; homolog of human RagC and RagD proteins; component of the EGO complex, which is involved in the regulation of microautophagy similar to Gtr1|small GTPase (putative) [UGA4] Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane GABA-specific transport protein YNL279W [PRM1] Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Null mutant is viable but exhibits a mating defect. [ERG3] C-5 sterol desaturase, catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources C-5 sterol desaturase Null mutant is inviable; suppresses syringomycin resistant mutant; sensitive to photoactivated 3-carbethoxypsoralen, UV light, radiomimetic mutagens, and oxidative stress

YLR292C
[SEC72] Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER YIL162W [SUC2] Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively invertase (sucrose hydrolyzing enzyme) Null mutant is viable but cannot ferment sucrose

YBR171W
[SEC66] Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER glycoprotein complexed with Sec62p and Sec63p in the Sec63 complex, an integral endoplasmic reticulum membrane protein complex required for translocation of presecretory proteins

YPL094C
[SEC62] Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER ER protein translocation apparatus membrane component secretion deficient

YJL002C
[OST1] Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins 64 kDa, alpha subunit of oligosaccharyltransferase complex; homologous to mammalian ribophorin I Null mutant is inviable; temperature-sensitive mutants show pleiotropic underglycosylation of soluble and membrane-bound glycoproteins

YOR103C
[OST2] Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins 40% identical to vertebrate DAD1 protein|oligosaccharyltransferase complex 16 kDa epsilon subunit Null mutant is inviable; overexpression of OST2 suppresses temperature-sensitivity of wbp1-2 mutant; conditional mutants show pleiotropic underglycosylation of soluble and membrane-bound glycoproteins

YOR085W
[OST3] Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins oligosaccharyl transferase glycoprotein complex 34 kDa gamma subunit Null mutant is viable but shows underglycosylation of soluble and membrane-bound glycoproteins and contains less oligosaccharyltransferase activity in vitro

YGL022W
[STT3] Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis integral ER membrane protein|oligosaccharyltransferase complex subunit (putative) Null mutant is inviable. sst3 mutants are defective in protein glycosylation, sensitive to hygromycin B, and resistant to sodium orthovanadate. Depletion of the STT3 protein results in loss of oligosaccharyl transferase activity in vivo and a deficiency in the assembly of oligosaccharyl transferase complex.

YML019W
[OST6] Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p N-oligosaccharyltransferase complex 37kDa subunit (putative) [AQY1] Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance aquaporin Null mutant is viable and exhibits improved viability when grown under hypo-osmolar or hyper-osmolar stress.

YPL274W
[SAM3] High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p high affinity S-adenosylmethionine permease Null mutant is viable but has inability to use Sadenosylmethionine as a sulfur source YBR283C [SSH1] Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Null mutant is viable, but grows slowly YBR069C [TAT1] Amino acid transport protein for valine, leucine, isoleucine, and tyrosine, low-affinity tryptophan and histidine transporter; overexpression confers FK506 resistance amino acid transport protein for valine, leucine, isoleucine, and tyrosine overexpression confers resistance to the volatile anesthetic isoflurane.

YGL051W
[MST27] Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles protein with COPI and COPII bindng motifs

YER026C
[CHO1] Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline phosphatidylserine synthase The null mutant is viable but grows slowly on minimal medium. The growth rate of the null mutant on minimal medium can be increased by supplementing the medium with choline or other phospholipid precursors.

YPL227C
[ALG5] UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum UDP-glucose:dolichyl-phosphate glucosyltransferase underglycosylation of carboxypeptidase Y YDR307W YHL042W YCL025C [AGP1] Low-affinity amino acid permease with broad substrate range, involved in uptake of asparagine, glutamine, and other amino acids; expression is regulated by the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p) amino acid permease Null mutant is viable; resistant to the amino acid analog gamma-hydroxyaspartate, decreased growth on asn, gln and some other amino acids in strains in which Gap1 and Gnp1 are also missing.

YGR260W
[TNA1] High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) high affinity nicotinic acid plasma membrane permease Null mutant is viable; the deletion of both YGR260W and YJR025C/BNA1 is lethal at low external nicotinic acid concentration YPR028W [YOP1] Membrane protein that interacts with Yip1p to mediate membrane traffic; overexpression results in cell death and accumulation of internal cell membranes; regulates vesicular traffic in stressed cells

YMR058W
[FET3] Ferro-O2-oxidoreductase required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases multicopper oxidase The null mutant is viable but defective for high affinity Fe(II) uptake. The null mutant is inviable when environmental iron is limiting. [ERV29] Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo ER-Golgi transport vesicle protein YLL028W [TPO1] Polyamine transporter that recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane YLR018C [POM34] Integral membrane protein of the nuclear pore complex, localizes adjacent to the nuclear membrane integral membrane protein|nuclear pore complex subunit YJR117W [STE24] Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans zinc metallo-protease Null mutant is viable, exhibits a mating efficiency of ~5% that of a wild-type strain and an a-factor processing defect [TRS130] One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1

YML077W
[BET5] Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi TRAPP 18kDa component

YGR166W
[KRE11] Protein involved in biosynthesis of cell wall beta-glucans; subunit of the TRAPP (transport protein particle) complex, which is involved in the late steps of endoplasmic reticulum to Golgi transport Null mutant is viable; killer toxin resistant; reduced levels of 1,6-betaglucan in cell wall

YDR108W
[GSG1] Subunit of TRAPP (transport protein particle), a multi-subunit complex involved in targeting and/or fusion of ER-to-Golgi transport vesicles with their acceptor compartment; protein has late meiotic role, following DNA replication

YDR407C
[TRS120] One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic

YDR246W
[TRS23] One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4

YOR115C
[TRS33] One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic

YDR472W
[TRS31] One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic

YBR254C
[TRS20] One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder

YKR068C
[BET3] Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex transport protein particle (TRAPP) component [MIA40] Essential protein of the mitochondrial intermembrane space, involved in import and assembly of intermembrane space proteins YOR132W [VPS17] Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation Null mutant is viable, exhibits defect in vacuolar morphology and protein sorting

YHR012W
[VPS29] Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Defective for sorting of soluble hydrolases to the vacuole. Mislocalisation of the vacuolar hydrolase sorting receptor Vps10p.

YOR069W
[VPS5] Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p simialr to sorting nexin I Null mutant missorts and secretes soluble vacuolar proteins, contains fragmented vacuoles, and mislocalizes carboxypeptidase and Vps10p.

YJL053W
[PEP8] Vacuolar protein sorting protein that forms part of the multimeric membrane-associated retromer complex along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport vacuolar protein similar to mouse gene H<beta>58 Null mutant is viable but is defective in processing of soluble vacuole proteases due to inability of soluble vacuolar hydrolase to reach the vacuole

YJL154C
[VPS35] Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps26p and Vps29p that selects cargo proteins for endosome-to-

YDR080W
[VPS41] Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Null mutant is viable, associated with fragmented vacuoles, exhibits defective high affinity transport due to impaired Fet3p activity and also exhibits defects in the processing and sorting of multiple vacuolar hydrolases YDL077C [VAM6] Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Null mutant is viable but exhibits defects in processing vacuolar proteases and in maturation of vacuolar alkaline phosphatase. Mutants also exhibit a defective vacuolar morphology; they contain several small vesicles that stain with vacuolar markers.

YLR396C
[VPS33] ATP-binding protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at both the Golgi-to-endosome and endosome-to-vacuole stages of protein transport temperature sensitive, defective vacuolar morphology and protein localization, methionine auxotroph YPL045W [VPS16] Subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; part of the Class C Vps complex essential for membrane docking and fusion at both the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Null mutant is viable, has a severe defect in vacuolar protein sorting, is temperature sensitive for growth, displays grossly abnormal vacuolar morphology, and possesses a defect in alpha-factor processing [GGA1] Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi ARF-binding protein Single and double knockouts are viable at both 30 C and 37 C. Cells lacking GGA1, GGA2 exhibit defects in invertase processing, vacuolar morphology, maturation of alpha-factor, and sorting of CPY, proteinase A to the vacuole, but not endocytosis.

YDL192W
[ARF1] ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p ADP-ribosylation factor Null mutant is viable and shows slow growth, cold sensitivity and sensitivity to normally sublethal concentrations of fluoride ion in the medium.

YLR330W
[CHS5] Protein of unknown function, involved in chitin biosynthesis by regulating Chs3p localization, also involved in cell fusion during mating YMR237W [BCH1] Protein that colocalizes with clathrin-coated vesicles; involved in transport at the trans-Golgi [ADH1] Alcohol dehydrogenase, fermentative isozyme active as homo-or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway alcohol dehydrogenase Null mutant is viable and sensitive to formaldehyde.

YDR189W
[SLY1] Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex t-SNARE-interacting protein that functions in ER-to-Golgi traffic Null mutant is inviable; SLY1-20, which differs from wild-type SLY1 by a single amino acid, is a single copy suppressor of loss of YPT1

YIL004C
[BET1] Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins YNL287W [SEC21] Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo PEST sequence-containing protein|non-clathrin coat protein

YGL137W
[SEC27] Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP yeast coatomer beta'-subunit

YDL145C
[COP1] Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway coatomer complex gamma-alpha-COP alpha subunit Null mutant is inviable; other cop1 alleles show secretion and protein sorting defects

YDR238C
[SEC26] Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) yeast coatomer subunit

YIL076W
[SEC28] Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth epsilon-COP coatomer subunit

YFR051C
[RET2] Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER ret2-1 mutant is thermosensitive and shows defects in retrieval of dilysine-tagged proteins from the Golgi back to the ER and, at the non-permissive temperature, in forward ER-to-Golgi transport

YPL010W
[RET3] Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER vesicle coat component ret3-1 mutant is thermosensitive and shows defects in retrieval of dilysine-tagged proteins from the Golgi back to the ER

YHR005C-A
[MRS11] Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that mediates insertion of hydrophobic proteins at the inner membrane, has homology to Mrs5p, which is also involved in this process Null mutant is inviable; depletion of Mrs11p results in accumulation of the precursor form of mitochondrial hsp60, inability to form spectrophotometrically detectable amounts of cytochromes and changes in the mitochondrial morphology; when overexpressed, restores respiration competence to yeast defective in the splicing of mitochondrial group II introns YOR297C [TIM18] Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane; may function to stabilize the complex translocase

YJL054W
[TIM54] Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane Null mutant is inviable; the tim54-1 allele is temperature-sensitive and at the nonpermissive temperature is defective in the insertion of proteins into the mitochondrial inner membrane.

YBR091C
[MRS5] Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twinpore translocase that mediates insertion of numerous multispanning inner membrane proteins Null mutant is inviable. Mrs5p depletion causes accumulation of unprocessed precursors of the mitochondrial hsp60 protein and defects in all cytochrome complexes

YDL217C
[TIM22] Component of the mitochondrial Tim54p-Tim22p complex involved in insertion of polytopic proteins into the inner membrane 0089 GO_TERM:[mitochondrial outer membrane translocase complex] P-Value:6.9e-14 OVERLAP:[TOM -transport across the outer membrane] <290.10> SIZE:9 YDR375C [BCS1] Protein of the mitochondrial inner membrane that functions as an ATP-dependent chaperone, required for the assembly of the cytochrome bc(1) complex from the Rip1p and Qcr10p proteins; member of the CDC48/PAS1/SEC18 ATPase family ATPase (AAA family) Gross reduction in the Rieske iron-sulfur subunit

YPR133W-A
[TOM5] Small mitochondrial outer membrane protein crucial to a binding relay for the import of proteins into mitochondria; subunit on the outer mouth of the TOM channel that accepts precursors from the receptors Tom20p and Tom22p Null mutant is viable but is temperaturesensitive and shows defects in import of mitochondrial preproteins; synthetically lethal with tom6, tom7, tom20, tom37, and tom70

YNL131W
[TOM22] Component of the TOM (translocase of outer membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between the TOM and TIM complexes mitochondrial import receptor protein

YGR082W
[TOM20] Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins 20 kDa mitochondrial outer membrane protein import receptor

YMR203W
[TOM40] Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore mitochondrial outer membrane protein Null mutant is inviable; cells accumulate uncleaved mitochondrial precursor proteins

YNL121C
[TOM70] Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins 70 kDa mitochondrial specialized import receptor of the outer membrane Null mutant is viable but exhibits defects in mitochondrial import [KOG1] Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors YPL180W [TCO89] Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Null: Caffeine Sensitivity.

YJR066W
[TOR1] PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis phosphatidylinositol kinase homolog Null mutant is viable, grows slowly; rapamycin resistance, tor1 tor2 double mutant is inviable YER040W [GLN3] Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source transcriptional activator of nitrogen-regulated genes YNL229C [URE2] Nitrogen catabolite repression regulator that acts by inhibition of GLN3 transcription in good nitrogen source; altered form of Ure2p creates [URE3] prion prion|transcriptional regulator Null mutant is viable but exhibits defects in nitrogen catabolite repression (NCR), and null mutant diploids are defective in pseudohyphal growth and display an increased incidence of random bud patterns. [GRX5] Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; mitochondrial matrix protein involved in the synthesis/assembly of iron-sulfur centers; monothiol glutaredoxin subfamily member along with Grx3p and Grx4p glutaredoxin Null mutant is viable and shows high sensitivity to oxidative stress and increased sensitivity to osmotic stress, and increased oxidation levels of cell proteins; grx5 is synthetically lethal with grx2.

YNL047C
[SLM2] Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; subunit of and phosphorylated by the TORC2 complex YIL105C [SLM1] Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; subunit of and phosphorylated by the TORC2 complex , a membrane-associated complex that regulates cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity YNL006W [LST8] Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Reduced activity of a broad set of amino acid permeases YKL203C [TOR2] PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis Null mutant is inviable, exhibits disruption of the polarized distribution of the actin cytoskeleton during the cell cycle; tor2-1 allele confers rapamycin resistance [MSS51] Nuclear encoded protein required for translation of COX1 mRNA; binds to Cox1 protein necessary for cox1 pre-mRNA processing and translation YML129C [COX14] Mitochondrial membrane protein, required for assembly of cytochrome c oxidase mitochondrial membrane protein Nuclear respiration deficient, lack cytochromes a and a3 and detectable cytochrome oxidase activity 0099 GO_TERM:[mitochondrial electron transport, cytochrome c to oxygen] P-Value:1.6e-13 OVERLAP:[Cytochrome c oxidase (complex IV)] <420.40> SIZE:11 Q0045 [COX1] Subunit I of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits cytochrome c oxidase subunit I

Q0275
[COX3] Subunit III of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits cytochrome c oxidase subunit III YER154W [OXA1] Translocase of the mitochondrial inner membrane, mediates the insertion of both mitochondrial-and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; null is respiratory deficient

YDL067C
[COX9] Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain cytochrome c oxidase subunit VIIa Lacks functional cytochrome c oxidase holoenzyme

YGL187C
[COX4] Subunit IV of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; N-terminal 25 residues of precursor are cleaved during mitochondrial import cytochrome c oxidase subunit IV

YNL052W
[COX5A] Subunit Va of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth cytochrome c oxidase subunit Va Null mutant is viable, respires at 10-15% of the wild-type rate due to the presence of COX5B; cox5a cox5b double deletion mutants are completely non-respiratory [NDJ1] Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution (interference), sister chromatid cohesion at meiotic telomeres, and segregation of small chromosomes Null allele exhibits errors in meiotic chromosome segregation about 10-fold higher than the wild-type error rate. Spore viability of homozygous diploids with the null allele is approximately 50% of wild-type. Mutant also shows delayed meiotic chromosome synapsis, disrupted crossover interference and increased frequency of nonexchange chromosomes leading to meiosis I nondisjunction and disruption of distributive disjunction YCR091W [KIN82] Putative serine/threonine protein kinase, most similar to cyclic nucleotide-dependent protein kinase subfamily and the protein kinase C subfamily serine/threonine kinase (putative)|similar to cyclic nucleotide-dependent protein kinase subfamily and the protein kinase C subfamily [PBP2] RNA binding protein with similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length YNL054W-A

YNL001W
[DOM34] Probable RNA-binding protein, functions in protein translation to promote G1 progression and differentiation, required for meiotic cell division YGR094W [VAS1] Mitochondrial and cytoplasmic valyl-tRNA synthetase valine-tRNA ligase YMR295C YOR196C [LIP5] Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase lipoic acid synthase Null mutant is viable; cannot synthesize lipoic acid; grows slowly on ethanol-rich media; barely grows on glyerol-rich media; undergoes a high frequency of mitochondrial DNA deletions YNL022C YJL095W [BCK1] Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p MEKK Null mutants are temperature-sensitive and exhibit cell lysis, which can be rescued by 1M sorbitol; null mutants grow very poorly even at the permissive temperature. Some dominant alleles suppress a pkc1 null mutant.

YKL168C
[KKQ8] Putative serine/threonine protein kinase with unknown cellular role YNL073W [MSK1] Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria lysine-tRNA ligase An uncharacterized allele is respiratory deficient.

YMR285C
[NGL2] Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p and Ngl3p RNase YBR086C [IST2] Plasma membrane protein that may be involved in osmotolerance, localizes to the mother cell in small-budded cells and to the bud in medium-and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process YDL173W YBL057C [PTH2] One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for cell growth YBL010C YBL064C [PRX1] Mitochondrial peroxiredoxin (1-Cys Prx) with thioredoxin peroxidase activity, has a role in reduction of hydroperoxides; induced during respiratory growth and under conditions of oxidative stress peroxiredoxin YDL201W [TRM8] Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA YMR291W [CHS1] Chitin synthase I, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor chitin synthase 1 YHR082C [KSP1] Nonessential putative serine/threonine protein kinase of unknown cellular role; overproduction causes allele-specific suppression of the prp20-10 mutation YNL154C [ [IDS2] Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Null mutations reduce or abolish the ability of IME2p to activate expression of early, middle, and late meiotic genes. Recessive and null ids2 mutants prevent toxicity of Ime2p expression in rad52 haploids, but do not affect Ime2p polypeptide accumulation.

YOR351C
[MEK1] Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, phosphorylates Red1p, interacts with Hop1p; required for meiotic recombination and normal spore viability meiosis-specific serine/threonine protein kinase Null mutant is viable, however diploids homozygous for a mek1 null mutation produce only low percentages of viable spores, reduced spore viability is rescued by spo13 mutations

YIL072W
[HOP1] Meiosis-specific DNA binding protein that displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for homologous chromosome synapsis and chiasma formation DNA binding protein decreased levels of meiotic crossing over and intragenic recombination between markers on homologous chromosomes

YLR263W
[RED1] Protein component of the axial elements of the synaptonemal complex, involved in chromosome segregation during the first meiotic division; interacts with Hop1p; required for wild-type levels of Mek1p kinase activity meiosis-specific component of synaptonemal complex axial element protein core YGL134W [PCL10] Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate YKR058W [GLG1] Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin glycogen synthesis initiator Null mutant is viable; disruption of both GLG1 and GLG2 renders cells unable to synthesize glycogen YLR258W [GSY2] Glycogen synthase, similar to Gsy1p; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase glycogen synthase (UDPglucose-starch glucosyltransferase) Null mutant is viable. Mutant lacking both GSY1 and GSY2 is viable but lacks glycogen synthase activity and glycogen deposition YFR015C [GSY1] Glycogen synthase with similarity to Gsy2p, the more highly expressed yeast homolog; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase glycogen synthase (UDP-glucose-starch glucosyltransferase) Null mutant is viable. Mutant lacking both GSY1 and GSY2 is viable but lacks glycogen synthase activity and glycogen deposition YJL137C [GLG2] Self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin glycogen synthesis initiator Null mutant is viable; disruption of both GLG2 and GLG2 renders cells unable to synthesize glycogen 0106 GO_TERM:[alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)] P-Value:8.1e-12

YDR074W
[TPS2] Phosphatase subunit of the trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway trehalose-6-phosphate phosphatase Null mutant is viable, exhibits complete loss of trehalose-6-phosphate phosphatase activity, measured in vitro, and accumulation of excessive amounts of trehalose-6-phosphate instead of trehalose upon heat shock or entrance into stationary phase in vivo; null mutant is temperature sensitive, tps2 (pfk3) pfk1 double mutants are glucose negative YMR261C [TPS3] Regulatory subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway trehalose-6-phosphate synthase/phosphatase complex 115 kDa regulatory subunit YBR126C [TPS1] Synthase subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway trehalose-6-phosphate synthase/phosphatase complex 56 kDa synthase subunit null is viable, but does not grow on glucose and/or fructose,and shows lack of trehalose YML100W [TSL1] Large subunit of trehalose 6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex, which converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose, homologous to Tps3p and may share function similar to TPS3 gene product|trehalose-6-phosphate synthase/phosphatase complex 123 kDa regulatory subunit [ARC1] Protein that binds tRNA and methionyl-and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids Null mutant is viable, leads to slow growth and reduced MetRS activity; arc1-mutants are synthetic lethals and are complemented by the genes for methionyl-tRNA and glutamyl-tRNA synthetase.

YGR264C
[MES1] Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs methionine-tRNA ligase no growth at 36 degrees C [YAK1] Serine-threonine protein kinase that is part of a glucose-sensing system involved in growth control in response to glucose availability; translocates from the cytoplasm to the nucleus and phosphorylates Pop2p in response to a glucose signal viable, confers growth to strains deleted for tpk1, tpk2, tpk3 (genes encoding the catalytic subunit of the cAMP-dependent kinase)

YLR270W
[DCS1] Non-essential hydrolase involved in mRNA decapping, may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p [MSD1] Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene aspartyl-tRNA synthetase Null mutant is viable but shows pleiotropic phenotypes consistent with a lesion in mitochondrial protein synthesis and is unable to acylate mitochondrial aspartyl-tRNA 0111 GO_TERM:[phosphotransferase activity, alcohol group as acceptor] P-Value:7.2e-04

YBR059C
[AKL1] Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization YLL018C [DPS1] Cytoplasmic aspartyl-tRNA synthetase, homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation aspartyl-tRNA synthetase YGR038W [ORM1] Evolutionarily conserved protein with similarity to Orm2p, required for resistance to agents that induce the unfolded protein response; human ortholog is located in the endoplasmic reticulum YNR039C [ZRG17] Endoplasmic reticulum protein of unknown function, transcription is induced under conditions of zinc deficiency; mutant phenotype suggests a role in uptake of zinc YBR157C [ICS2] Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization YDL223C [HBT1] Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis [RGT1] Glucose-responsive transcription factor that regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor transcriptional activator|transcriptional repressor Null mutant is viable, shows consitutive expression of glucose-induced HXT geness

YIL033C
[BCY1] Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation cAMP-dependent protein kinase regulatory subunit Null mutant is viable; sra1 mutants are associated with reduction of glycogen accumulation, temperature sensitivity, reduced growth rate on maltose and sucrose, inability to grow on galactose and nonfermentable carbon sources and nitrogen starvation intolerance. Cells lacking Sra1p are constitutive for cAPK activity resulting in meiotic arrest prior to premeiotic DNA synthesis YNL227C [JJJ1] Protein that contains a 70 amino acid J-domain, may function as a co-chaperone to recruit Hsp70-like activity to specific sites; mutation causes defects in fluid-phase endocytosis

YJL164C
[TPK1] Subunit of cytoplasmic cAMP-dependent protein kinase, which contains redundant catalytic subunits Tpk1p, Tpk2p, and Tpk3p and regulatory subunit Bcy1p; promotes vegetative growth in response to nutrients; inhibits filamentous growth cAMP-dependent protein kinase catalytic subunit (putative) multicopy suppression of ras mutant [RAV1] Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate

YKL080W
[VMA5] Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane V1 sector hydrophilic subunit C|vacuolar ATPase V1 domain subunit C (42 kDa)|vacuolar H-ATPase Null mutant is viable; certain vma5 mutations show allelespecific synthetic lethality with cdc24-ls mutants

YOR270C
[VPH1] Subunit of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes V0 sector subunit|essential for vacuolar acidification and vacuolar H-ATPase activity|vacuolar ATPase V0 domain subunit a (100 kDa)|vacuolar H-ATPase Null mutant is viable, deficient in assembly of vacuolar H(+) ATPase and acidification of the vacuole

YBR127C
[VMA2] Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm vacuolar ATPase V1 domain subunit B (60 kDa) Null mutant is viable, severely defective for growth in medium buffered at neutral pH

YDL185W
[TFP1] Vacuolar ATPase V1 domain subunit A containing the catalytic nucleotide binding sites; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease protein with three regions (ABC) that are spliced to yield the extein AC and the intein B; AC is a 69K vacuolar (H+)-ATPase, and B is a 50K site-specfic endonuclease named VDE (PI-SceI) that is homologous to HO. Cleavage is meiosis-specific and induces ge|site-specific endonuclease VDE (PI-SceI)|vacuolar ATPase V1 domain subunit A (69 kDa) Null mutant is viable, resistant to trifluoperazine, grows slowly under non-acidic conditions and on glycerol and is cold, temperature, and cation-sensitive

YOR332W
[VMA4] Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane E subunit of V1 sector|vacuolar H(+) ATPase 27 kDa subunit Null mutant is viable, slow growing, cold-sensitive, thermo-sensitive, and exhibits poor growth on glycerol; fails to grow on media supplemented with 100 mM CaCl2 or ZnCl2

YEL051W
[VMA8] Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis V1 catalytic sector D subunit|vacuolar H-ATPase Null mutant is viable, does not grow on media buffered at pH 7.5 and does not show accumulation of quinacrine into its vacuoles; grows slowly, fails to grow on non-fermentable carbon sources

YMR054W
[STV1] Subunit of vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole 110 kDa subunit; not in vacuole membrane|vacuolar H-ATPase Null mutant is viable, displays additive phenotypes in combination with vph1 null mutations

YGR020C
[VMA7] Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane vacuolar ATPase V1 domain subunit F (14 kDa) Null mutant is viable, unable to grow on media buffered at pH 7.5, fails to accumulate quinacrine into vacuoles, other subunits of the catalytic sector are not assembled onto the vacuolar membrane

YHR039C-A
[VMA10] Vacuolar H+ ATPase subunit G of the catalytic (V1) sector, involved in vacuolar acidification vacuolar ATPase V1 domain subunit G (13 kDa) Null mutant is viable, fails to grow on media buffered at pH 7.5, fails to accumulate quinacrine in its vacuole

YPR036W
[VMA13] Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase vacuolar H(+) ATPase V1 sector 54 kDa subunit Null mutant is viable, V-ATPase complex from null mutants is less stable than from wild-type strains 0117 GO_TERM:[mitochondrion] P-Value:2.4e-01

YEL030W
[ECM10] Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations A Tn3 insertion into this gene causes hypersensitivity to the cell surface polymer perturbing agent calcofluor white.

YJR062C
[NTA1] Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation 52 kDa amidase specific for N-terminal asparagine and glutamine Null mutant is viable but cannot degrade N-end rule substrates that have N-terminal asparagine or glutamine 0118 GO_TERM:[response to stress] P-Value:4.0e-02

YKL210W
[UBA1] Ubiquitin activating enzyme, involved in ubiquitin-mediated protein degradation and essential for viability ubiquitin activating enzyme e1 YMR108W [ILV2] Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control acetolactate synthase Isoleucine-plusvaline requiring; Sulfometuron methyl resistance YEL060C [PRB1] Vacuolar proteinase B (yscB), a serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation vacuolar protease B Null mutant is viable, protease B deficient, has smaller spores than wildtype embedded in a thick matrix YAL015C [NTG1] DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes to the nucleus and mitochondrion DNA glycosylase Null mutant is viable but is sensitive to H202 and menadione YDL059C [RAD59] Protein involved in the repair of double-strand breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; homologous to Rad52p the RAD59 gene product has homology to the Rad52 protein gamma-ray sensitivity, mitotic recombination defects. rad59 is epistatic to rad52 for its repair and recombination defects.

YDR054C
[CDC34] Ubiquitin-conjugating enzyme or E2; together with Skp1p, Rbx1p, Cdc53p, and an F-box protein, forms a ubiquitin-protein ligase called the SCF complex which regulates cell cycle progression by targeting key substrates for degradation ubiquitin-conjugating enzyme overexpression confers resistance to xenobiotics (methylmercury, mercuric chloride, and p-chloromercuribenzoate).

YDL132W
[CDC53] Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Cells arrest in G1 with active Cln kinases but no Clb-associated Cdc28p kinase activity

YDR328C
[SKP1] Evolutionarily conserved kinetochore protein that is part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase Null mutant is inviable, temperature-sensitive mutations in SKP1 arrest in G1 or G2

YPR007C
[REC8] Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Null mutant is viable, does not undergo meiotic division and is unable to sporulate. The null mutant also exhibits a loss of sister chromatid cohesion, an absence of the synaptonemal complex, and chaotic chromosome segregation.

YDR180W
[SCC2] Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX

YJL074C
[SMC3] Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member SMC chromosomal ATPase family member

YFL008W
[SMC1] Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure SMC chromosomal ATPase family member null is inviable; other mutants show chromosome loss and defects in nuclear division

YDL003W
[MCD1] Essential protein required for sister chromatid cohesion in mitosis and meiosis; subunit of the cohesin complex; expression is cell cycle regulated and peaks in S phase Null mutant is inviable; temperature sensitive mutants are defective in mitotic sister chromatid cohesion and mitotic chromosome condensation; multicopy suppressor of smc1-2 mutation

YIL026C
[IRR1] Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability cohesin complex subunit Null mutant is inviable; decreased transcription of mutant causes irregularity of zygotes, colonies, decreased adhesion to solid supports [CBF1] Helix-loop-helix protein that binds the motif CACRTG, which is present at several sites including MET gene promoters and centromere DNA element I (CDEI); required for nucleosome positioning at this motif; targets Isw1p to DNA basic helix-loop-helix protein Null mutant is viable, but grows slowly and causes partial loss of centromere function (increased chromosome loss), benomyl and thiabendazole sensitivity, methionine auxotrophy, and changes in chromatin structure at CENs and some promoters. Null mutation causes precocious sister segregation at MI, and reduced spore viability.

YMR094W
[CTF13] Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis

YIR017C
[MET28] Transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism transcriptional activator in the Cbf1p-Met4p-Met28p complex Null mutant is viable but is a methionine-auxotroph and resistant to toxic analogs of sulfate.

YNL103W
[MET4] Lecine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p leucine zipper family|transcriptional activator

YOL069W
[NUF2] Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Null mutant is inviable; temperature-sensitive mutants arrest with single undivided or partially divided nucleus in the bud neck, shortened mitotic spindle, and fully replicated DNA

YER018C
[SPC25] Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering spindle pole component [TUB4] Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body gamma tubulin Null mutant is inviable. Tub4p-depleted cells arrest during nuclear division; most arrested cells contain a large bud, replicated DNA, and a single nucleus. Immunofluorescence and nuclear staining experiments indicate that cells depleted of Tub4p contain defects in the organization of both cytoplasmic and nuclear microtubule arrays; such cells exhibit nuclear migration failure, defects in spindle formation, and/or aberrantly long cytoplasmic microtubule arrays.

YLR200W
[YKE2] Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin bovine NABC complex component homolog|non-native actin binding complex polypeptide 6|prefoldin complex subunit YJL179W [PFD1] Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha-and gamma-tubulin bovine prefoldin subunit 1 homolog (putative)

YEL003W
[GIM4] Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it bovine prefoldin subunit 2 homolog (putative) Null mutant is viable, sensitive to anti-microtubule drugs benomyl and nocadazole; synthetically lethal with tub4-1 mutations

YNL153C
[GIM3] Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it bovine prefoldin subunit 4 homolog (putative)

YML094W
[GIM5] Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it bovine prefoldin subunit 5 homolog (putative) Null mutant is viable, cold sensitive, benomyl and nocadazole sensitive and fails to grow on YPD+1.2M KCl or YPD+1.8M sorbitol ; synthetically lethal with tub4-1 mutations [ADY4] Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane YDL239C [ADY3] Protein required for spore wall formation, thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p Null forms largely asci that contain 2 spores (dyads) when sporulated. Sporulation defect in ady3ady3 cells is due to a failure to synthesize spore wall polymers.

YNL225C
[CNM67] Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration Null mutant is viable but shows slow growth and a nuclear migration defect YOL091W [SPO21] Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation meiosis proficient, fails to form spores [CDC5] Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate protein kinase Null mutant is inviable. cdc5(ts) mutants form synaptonemal complexes lacking central elements and arrest either at meiosis I with broken spindles or at meiosis II with short spindles. Late shifts to a restrictive temperature result in reductional dyads; each spore contains an entire meiosis II short spindle with unseparated chromatids. In some strains at semi-permissive temperature, chromosomes segregate reductionally or equationally depending upon the centromere.

YGL003C
[CDH1] Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of mitotic cyclins resulting in exit from mitosis; targets the APC/C to specific substrates including CDC20, ASE1 and CIN8 required for Clb2 and Ase1 degradation Null mutant is viable but defective in Clb2p and Ase1p degradation; deletion of cdh1 causes pheromone resistance and is synthetically lethal with sic1 deletion; overexpression causes ectopic degradation of Clb2p and Ase1p

YFR036W
[CDC26] Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition thermosensitive cell growth (lethal at high temperature)

YNL172W
[APC1] Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition ubiquitin ligase subunit

YBL084C
[CDC27] Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase promoting complex (APC) subunit Null mutant is inviable. Some conditional alleles overreplicate their DNA.

YHR166C
[CDC23] Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition unable to complete G(sub)2/M transition

YKL022C
[CDC16] Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation metal-binding nucleic acidbinding protein, interacts with Cdc23p and Cdc27p to catalyze the conjugation of ubiquitin to cyclin B (putative) Null mutant is inviable; sensitive to caffeine; cdc16 mutants are unable to progress through the G(sub)2/M transition, cell division cycle blocked at 36 degrees C

YLR102C
[APC9] Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase promoting complex (APC) subunit Null mutant is viable at 37 C but show delay in entry into anaphase at 37 C

YOR249C
[APC5] Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase promoting complex (APC) subunit Null mutant is inviable at 25 C

YDL008W
[APC11] Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase promoting complex (APC) subunit Null mutant is inviable at 25 C

YLR127C
[APC2] Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; similar to cullin Cdc53p anaphase promoting complex (APC) subunit Null mutant is inviable at 25 deg. C; ts mutants arrest in metaphase due to defect in the degradation of Pds1; extracts from G1-arrested apc2 mutants are defective in the ubiquitination of mitotic cyclins

YDR118W
[APC4] Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase promoting complex (APC) subunit Null mutant is inviable at 25 C

YGL240W
[DOC1] Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis

YDR260C
[SWM1] Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Null mutant completes meiotic nuclear division but does not show spore wall maturation YIR025W [MND2] Subunit of the anaphase-promoting complex (APC); needed for meiotic nuclear division arrests after DNA-replication but before nuclear divisions after shift to sporulation medium

YJR086W
[STE18] G protein gamma subunit, forms a dimer with Ste4p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling G protein gamma subunit|coupled to mating factor receptor The null mutant is viable but sterile. Sst1 sst2 double mutants and scg1 mutants can be suppressed by a null allele of ste18.

YOR212W
[STE4] G protein beta subunit, forms a dimer with Ste18p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste18p to dampen signaling; may recruit Rho1p to the polarized growth site during mating; contains WD40 repeats G protein beta subunit|coupled to mating factor receptor YFL026W [STE2] Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells G protein coupled receptor (GPCR)|alphafactor pheromone receptor|seven-transmembrane domain protein

YHR005C
[GPA1] GTP-binding alpha subunit of the heterotrimeric G protein that couples to pheromone receptors; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates the pathway via Scp160p G protein alpha subunit|coupled to mating factor receptor The null mutation is inviable in haploids but not diploids. Gpa1 mutants exhibit specific defects in the pheromone responsiveness of both a and alpha cells.  [PRS2] 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a five related enzymes, which are active as heteromultimeric complexes ribose-phosphate pyrophosphokinase YKL181W [PRS1] 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes ribose-phosphate pyrophosphokinase YMR139W [RIM11] Protein kinase required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta Null mutant is viable; some alleles are Spo+ and sporulate slowly; rim11 is epistatic to the lethality of IME1 overexpression in haploids and permits Ime1p accumulation; RIM11 is a high copy suppressor of mck1 (cs) mutants YOL061W [PRS5] 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, involved in nucleotide, histidine, and tryptophan biosynthesis; one of a five related enzymes, which are active as heteromultimeric complexes phosphoribosylpyrophosphate synthetase (ribose-phosphate pyrophosphokinase) Null mutant is viable but reduces the cellular 5-phosphoribosyl-1(alpha)-pyrophosphate synthetase activity by 84%. prs5 mutations are synthetically lethal with mutations in prs1 or prs3.

YDL225W
[SHS1] One of five related septins (Cdc3p, Cdc10p, Cdc11p, Cdc12p, Shs1p) that form a cortical filamentous collar at the mother-bud neck which is necessary for normal morphogenesis and cytokinesis septin deficient for cytokinesis

YHR107C
[CDC12] Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM 10 nm filament component of mother-bud neck|septin abnormal cell-wall deposition and bud growth, inability to complete cytokinesis, failure to form the ring of 10nm filaments in the neck region of budding cells

YJR076C
[CDC11] Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM 10 nm filament component of mother-bud neck|septin abnormal cell-wall deposition and bud growth, inability to complete cytokinesis, failure to form the ring of 10nm filaments in the neck region of budding cells

YCR002C
[CDC10] Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM septin abnormal cell-wall deposition and bud growth, inability to complete cytokinesis, failure to form the ring of 10nm filaments in the neck region of budding cells

YLR314C
[CDC3] Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM septin Null mutant is inviable; other mutants show abnormal cell-wall deposition and bud growth, inability to complete cytokinesis, and failure to form the ring of 10nm filaments in the neck region of budding cells. [UFD2] Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 ubiquitin conjugating factor e4 Null mutant is viable but exhibits increased sensitivity to ethanol stress.

YIL030C
[SSM4] Ubiquitin-protein ligase of the ER/nuclear envelope, required for degradation of Alpha2p and other proteins containing a Deg1 degradation signal; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation integral membrane protein Null mutant is viable, suppresses temperature sensitive rna14 mutations; ssm4 sls1 mutants are inviable YMR297W [PRC1] Vacuolar carboxypeptidase Y (proteinase C), involved in protein degradation in the vacuole and required for full protein degradation during sporulation CPY|carboxypeptidase Y (proteinase C)|carboxypeptidase yscY Null mutant is viable,proteinase C deficient YDR057W [YOS9] Lectin; soluble lumenal ER protein; member of the OS-9 protein family; similar to mannose-6-phosphate receptors (MPRs); serves as a receptor that recognizes misfolded N-glycosylated proteins and participates in their targeting to ERAD membrane-associated glycoprotein Accelerates Gas1 transport and processing in cells overexpressing YOS9. Gas1 processing is slowed in cells bearing a deletion in YOS9.

YLR450W
[HMG2] One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks 3-hydroxy-3methylglutaryl-coenzyme A (HMG-CoA) reductase isozyme Null mutant is viable, sensitive to compactin, a competitive inhibitor of HMG-CoA reductase; hmg1 hmg2 double deletion mutants are inviable

YML029W
[USA1] Protein that interacts in the two-hybrid system with the U1 snRNP-specific protein, Snp1p; may have a role in pre-mRNA splicing pre-mRNA splicing factor (putative) YOL013C [HRD1] Ubiquitin-protein ligase required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger Null mutant is viable, slows degradation of Hmg2p

YBR201W
[DER1] Endoplasmic reticulum membrane protein, required for ER-associated protein degradation, involved in the retrograde transport of misfolded or unassembled proteins; N-and C-termini protrude into the cytoplasm, has similarity to Dfm1p Null mutant is viable, but blocks ER-degradation of target proteins YDL126C [CDC48] ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p and Ufd1p participates in retrotranslocation of ubiquitinated proteins from the ER into the cytosol for degradation by the proteasome YML013W [SEL1] UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p, has a ubiquitin-associated (UBA) domain, interacts with ubiquitylated proteins in vivo, and is required for degradation of a ubiquitylated model substrate Null: enhanced secretion YBR170C [NPL4] Endoplasmic reticulum and nuclear membrane protein, forms a complex with Cdc48p and Ufd1p that recognizes ubiquitinated proteins in the endoplasmic reticulum and delivers them to the proteasome for degradation Temperature-sensitive mutants accumulate nuclear-targeted proteins in the cytoplasm and poly(A)+RNA in the nucleus and show defects in nuclear membrane integrity at the nonpermissive temperature YGR048W [UFD1] Protein that interacts with Cdc48p and Npl4p, involved in recognition of polyubiquitinated proteins and their presentation to the 26S proteasome for degradation; involved in transporting proteins from the ER to the cytosol Homozygous ufd1-1 mutant diploids exhibit sporulation defects. loss of Ufd1 blocks ER-associated protein degradation at a post-ubiquitination but pre-proteasome step. [MSH1] DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis mutS homolog Null mutant is viable, petite

YHR024C
[MAS2] Larger subunit of the mitochondrial processing protease, essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins mitochondrial processing protease 53 kDa subunit

YLR163C
[MAS1] Smaller subunit of the mitochondrial processing protease, essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins mitochondrial processing protease subunit Null mutant is inviable; Elevated mitotic recombination and chromosomal missegregation when overproduced [RAD28] Protein involved in transcription-coupled repair nucleotide excision repair of UV-induced DNA lesions; homolog of human CSA protein Null mutant is viable but is hypermutable following exposure to UV light and is slightly more sensitive to UV light in the presence of mutations in rad7 or rad16

YDR212W
[TCP1] Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in maintenance of actin cytoskeleton; homolog to Drosophila melanogaster and mouse tailless complex polypeptide chaperonin subunit alpha YNL212W [VID27] Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasomedependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Null mutant is viable but exhibits vacuole degradation of cytosolic proteins

YIL142W
[CCT2] Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo YOR281C [PLP2] Essential protein with similarity to phosducins, which are GTPase inhibitors; lethality of null mutation is functionally complemented by expression of mouse phosducin-like protein MgcPhLP

YDL143W
[CCT4] Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo

YDR188W
[CCT6] Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif [CLC1] Clathrin light chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function, two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component clathrin light chain Null mutant is viable but slow-growing and shows defects in receptor-mediated endocytosis, maturation of alpha factor and levels of clathrin heavy chain (Chc1p); high copy suppresses the inviable double mutant chc1-delete, scd1-i-allele; elevated CHC1 expression suppresses some clc1-delete phenotypes YHR108W [GGA2] Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi ARF-binding protein Single and double knockouts are viable at both 30 C and 37 C. Cells lacking GGA1, GGA2 exhibit defects in invertase processing, vacuolar morphology, maturation of alpha-factor, and sorting of CPY, proteinase A to the vacuole, but not endocytosis.

YDR153C [ENT5] Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes;
associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin YGL206C [CHC1] Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; two heavy chains form the clathrin triskelion structural component; the light chain (CLC1) is thought to regulate function Clathrin heavy chain Null mutant is viable, but is slow-growing and shows defects in mating, sporulation and vesicle ultrastructure (however it shows little or no defect in secretion); null mutants easily become inviable due to second site mutations in a number of unlinked genes such as SCD1 and CDL1. Null mutants also exhibit an endocytosis defect, late Golgi protein mislocalization. chc1-5 exhibits delayed vacuolar protein transport.

YJR125C
[ENT3] Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p

YJL207C
[YJL207C] Protein of unknown function, proposed to function as a large AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; YJL207C is a non-essential gene YFR043C [YFR043C] Hypothetical protein; null mutant displays increased levels of spontaneous Rad52 foci YBR019C [GAL10] UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers UDP-glucose 4-epimerase Null mutant is viable and cannot utilize galactose.

YKL135C
[APL2] Beta-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting beta-adaptin|clathrin associated protein complex large subunit YFL034W YHL019C [APM2] Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport YLR170C [APS1] Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex clathrin associated protein complex small subunit Null mutant is viable; aps1 mutants demonstrate synthetic effects with chc1 alleles [SNF7] One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes YDR069C [DOA4] Ubiquitin hydrolase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole ubiquitin isopeptidase Null mutant is viable, but exhibits uncoordinated DNA replication|A nonsense mutation in the doa4-10 mutant eliminates the catalytic residues of the deubiquitinating enzyme while keeping the rhodanase domain intact. At 36 degrees C, this doa4-10 mutant exhibits increased sensitivity to camptothecin (CPT), osmotic stress, and hydroxyurea, and a reversible petite phenotype.

YPL084W
[BRO1] Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes YOR275C [RIM20] Protein involved in proteolytic activation of Rim101p in response to alkaline pH; member of the PalA/AIP1/Alix family; interacts with the ESCRT-III subunits Snf7p, suggesting a relationship between the response to pH and multivesicular body formation Null: Affected in sporulation and invasive growth. Other phenotypes: Alkaline sensitivity

YPR173C
[VPS4] AAA-type ATPase required for efficient late endosome to vacuole transport; catalyzes the release of an endosomal membraneassociated class E VPS protein complex; cytoplasmic protein that is also associated with an endosomal compartment AAA ATPase Null mutant is viable, exhibits protein sorting and morphological defects [BBC1] Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches YLR337C [VRP1] Proline-rich actin-associated protein involved in cytoskeletal organization and cytokinesis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) proline-rich protein verprolin Null mutant is viable but is both temperature and pH sensitive and cannot grow on minimal medium. Null mutant also exhibits depolarization of the actin cytoskeleton.

YKR069W
[MET1] S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in sulfate assimilation, methionine metabolism, and siroheme biosynthesis Null mutant is viable, and is a methionine auxotroph YOR247W [SRL1] Mannoprotein that exhibits a tight association with the cell wall, required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants YGL242C YIL156W [UBP7] Ubiquitin-specific protease that cleaves ubiquitin-protein fusions ubiquitin-specific protease YIR006C [PAN1] Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; previously thought to be a subunit of poly(A) ribonuclease Null mutant is inviable; conditional mutants show arrest of translation initiation, alterations in mRNA poly(A) tail lengths, and altered cellular location of Mod5p

YKL129C
[MYO3] One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization myosin I Null mutant is viable, myo3 myo5 double deletion mutants exhibit severe defects in growth and actin cytoskeletal organization YMR109W [MYO5] One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization myosin I Null mutant is viable; myo3 myo5 double deletion mutants exhibit loss of actin polarity, growth arrest at 37 degrees or high osmotic strength, accumulation of intracellular membranes, and loss of polarized cell surface growth; myo3 myo5 double mutants have longer doubling times and thicker cell walls

YDL029W
[ARP2] Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity actin related protein cells with mutations in Arp2 and Arc15 are defective in mitochondrial movement.

YJR065C
[ARP3] Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Mutations in Arp3 lead to defects in actin-patch motility and a rearrangement of the cortical actin cytoskeleton.

YBR234C
[ARC40] Essential subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches

YNR035C
[ARC35] Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Null mutant exhibits severe growth defects; synthetic lethal with vma2.

YKL013C
[ARC19] Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Null mutant is viable, but exhibits severe growth defects YIL062C [ARC15] Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Null mutant exhibits severe growth defects. Cells with mutations in Arp2 and Arc15 are defective in mitochondrial movement.

YLR370C
[ARC18] Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches

YCR088W
[ABP1] Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization actin binding protein

YOR181W
[LAS17] Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) actin assembly factor Null mutant is viable, demonstrates impaired budding and cytokenesis and severely disrupted cortical actin; other mutants accumulate secretory vesicles in the bud [TRM1] tRNA methyltransferase, localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments N2,N2-dimethylguanosine-specific tRNA methyltransferase An uncharacterized allele affects a specific base modification of both cytoplasmic and mitochondrial tRNA.

YGR121C
[MEP1] Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease YEL017W [GTT3] Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery

YJL173C
[RFA3] Subunit of heterotrimeric Replication Factor A (RF-A), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination replication factor-A subunit 3 Null mutant is inviable and arrests as budded and multiply budded cells

YNL312W
[RFA2] Subunit of heterotrimeric Replication Factor A (RF-A), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination 29% identical to the human p34 subunit of RF-A|replication factor RF-A subunit 2 Null mutant is inviable; arrests as budded and multiply budded cells; rfa2 (ts) cells have a mutator and a hyper-recombination phenotype and are more sensitive to hydroxyurea and methyl-methane-sulfonate than wild-type cells [GET3] ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress Null: YDL100c gene disruption results in sensitivity to As(III), As(V), Co(II) and Cu(II).

YER083C
[GET2] Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function null is hypersensitive to calcofluor white suffer an increased spheroplast lysis rate YGL020C [GET1] Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Null: Required for spore wall formation, but not IME1 induction or nuclear division

YDL064W
[UBC9] SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S-and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) SUMOconjugating enzyme YCR066W [RAD18] Protein involved in postreplication repair; binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; forms heterodimer with Rad6p; contains RING-finger motif ATPase (putative)|zinc finger protein Radiation-sensitive. mgs1 exhibits a synergistic growth defect with rad18. Growth defects of mgs1 rad18 double mutants are suppressed by a mutation in SRS2 or by overexpression of Rad52.|Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination.

YLR032W
[RAD5] Single-stranded DNA-dependent ATPase, involved in postreplication repair; contains RING finger domain ATPase (putative)|DNA helicase (putative) Radiation-sensitive. mgs1 exhibits a synergistic growth defect with rad5. mgs1 rad5 double mutant has increased sensitivity to hydroxyurea and a greatly increased spontaneous mutation rate.|Deletion mutants of this post-replication repair (PRR) gene do not have any cross-link-induced mutations but show increased levels of recombination. [ASF1] Nucleosome assembly factor, involved in chromatin assembly after DNA replication, anti-silencing protein that causes derepression of silent loci when overexpressed YBR088C [POL30] Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Proliferating Cell Nuclear Antigen (PCNA)

YOR144C
[ELG1] Protein required for S phase progression and telomere homeostasis, forms an alternative replication factor C complex important for DNA replication and genome integrity; mutants are sensitive to DNA damage YHR191C [CTF8] Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion

YOR217W
[RFC1] Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon replication factor C subunit 1|similar to human RFC 140 kDa subunit Null mutant is inviable, rfc1 conditional mutants arrest before mitosis YER173W [RAD24] Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein cell cycle exonuclease (putative) radiation sensitive YFR027W [ECO1] Acetyltransferase required for the establishment of sister chromatid cohesion during DNA replication, but not for its maintenance during G2 and M phases; also required for postreplicative double-strand break repair; interacts with Chl1p YMR078C [CTF18] Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Null mutant is viable, exhibits increased level of spontaneous mitotic recombination, slow growth, and cold sensitivity

YJR068W
[RFC2] Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon replication factor C subunit 2|similar to human RFC 37 kDa subunit

YBR087W
[RFC5] Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon replication factor C subunit 5|similar to human RFC 38 kDa subunit

YNL290W
[RFC3] Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon replication factor C subunit 3|similar to human RFC 36 kDa subunit

YOL094C
[RFC4] Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon replication factor C subunit 4|similar to human RFC 40 kDa subunit

YML095C
[RAD10] Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and doublestrand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein ssDNA endonuclease radiation sensitive|Deletion of this nucleotide excision repair (NER) gene results in lower levels of cross-link-induced recombination but higher mutation frequencies than wild-type cells.

YPL022W
[RAD1] Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and doublestrand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein UV endonuclease radiation sensitive|Deletion of this nucleotide excision repair (NER) gene results in lower levels of cross-link-induced recombination but higher mutation frequencies than wild-type cells. [ATG11] Peripheral membrane protein required for delivery of aminopeptidase I (Lap4p) to the vacuole in the cytoplasm-to-vacuole targeting pathway; also required for peroxisomal degradation (pexophagy) Oligomeric, coiled-coil, peripheral membrane protein required for stable binding of precursor API to its target membrane. cvt9 is defective in maturation of the vacuolar protein, aminopeptidase I and exhibits minor defects in autophagy|cvt9 is defective in vacuolar delivery of aminopeptidase I and peroxisome degradation but is not needed for macroautophagy. The null mutant is viable and is relatively starvation-insensitive.

YPL174C
[NIP100] Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) large subunit of dynactin complex (putative) Null mutant is viable but exhibits slow growth and defects in partitioning into daughter cells.

YMR294W
[JNM1] Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B

YDR106W
[ARP10] Component of the dynactin complex, localized to the pointed end of the Arp1p filament; may regulate membrane association of the complex YHR129C [ARP1] Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Null mutant is viable, but both null mutations and overexpression lead to defects in spindle orientation and nuclear migration (during mitosis in arp1 mutants the nucleus fails to move into the neck). [KGD1] Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA alpha-ketoglutarate dehydrogenase Null mutant is viable but is deficient in alpha-ketoglutarate dehydrogenase, is therefore respiratory deficient, cannot grow on glycerol, and produces increased amount of organic acids during growth on glucose

YGR193C
[PDX1] Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core pyruvate dehydrogenase complex protein X component YDR430C [CYM1] Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology Metalloprotease

YBR221C
[PDB1] E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria pyruvate dehydrogenase beta subunit (E1 beta)

YER178W
[PDA1] E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA, regulated by glucose pyruvate dehydrogenase alpha subunit (E1 alpha) Null mutant is viable, exhibits reduced growth on glucose and increased formation of petites [UBP14] Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T ubiquitin-specific protease Null mutant is viable but show accumulation of free ubiquitin chains, which correlates with defects in ubiquitin-dependent proteolysis; overexpression of mutant or wild-type Ubp14p can inhibit protein degration too YOL113W [SKM1] Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; proposed to be a downstream effector of Cdc42p during polarized growth

YBL037W
[APL3] Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport clathrin associated protein complex large subunit YJR005W [APL1] Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex beta-adaptin|clathrin associated protein complex large subunit

YJR058C
[APS2] Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex clathrin associated protein complex small subunit null mutant is viable; slight effect on chc1-ts cell growth

YOL062C
[APM4] Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport clathrin associated protein complex medium subunit [MSL5] Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA [IST3] Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Null mutant is viable but exhibits slow growth and a pre-mRNA splicing defect in vivo and in vitro. Deletion caused an immediate and exclusive accumulation of a particle consistent with a pre-mRNA/penta-snRNP complex.

YGL174W
[BUD13] Subunit of the RES complex, which is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern Null mutant is viable; diploid null mutants exhibit unipolar budding and elongate phenotype.

YOL149W
[DCP1] Subunit of the Dcp1p-Dcp2p decapping enzyme complex, which removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p Null mutant is inviable in the FY1679 background, but viable, though grows slowly, in the CEN.PK141 background.

YCR077C
[PAT1] Topoisomerase II-associated deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; functionally linked to Pab1p Null mutant is viable; slow growth rate, reduced fidelity of chromosome segregation during both mitosis and meiosis; slower rate of deadenylation-dependent decapping of mRNAs and transcript-specific effects on mRNA decay rates.

YMR268C
[PRP24] Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling U4/U6 snRNP-associated protein defective in splicing YER146W [LSM5] Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA snRNP protein YJR022W [LSM8] Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA snRNP protein [NTR2] Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly YGR129W [SYF2] Component of the spliceosome complex involved in pre-mRNA splicing; involved in regulation of cell cycle progression YBR188C [NTC20] Member of a complex, including Prp19p, that binds to the spliceosome; required for pre-mRNA splicing splicing factor Null mutant is viable. ntc20 ntc30 double mutant is very sick and accumulates pre-mRNA. Null mutant is synthetically lethal with prp19.

YJR050W
[ISY1] Component of the spliceosome complex involved in pre-mRNA splicing, auxiliary splicing factor that may modulate Syf1p activity and help optimize splicing; isy1 syf2 double mutation activates the spindle checkpoint, causing cell cycle arrest [STO1] Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Large subunit of the nuclear cap-binding protein complex defective growth on fermentable carbon sources and supression of top1-hpr1 YDR235W [PRP42] U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats U1 snRNP protein|shares 50% sequence similarity with Prp39p U1 snRNP protein and has multiple copies of the crn-like TPR motif Null mutant is inviable; prp39-1 is a point mutant that is temperature-sensitive for pre-mRNA splicing YML046W [PRP39] U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats RNA splicing factor|U1 snRNP protein Temperature-sensitive mutant arrests at the nonpermissive temperature and shows block in pre-mRNA splicing YDL087C [LUC7] Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site Null mutant is inviable; luc7 mutants exhibit synthetic lethality with the Cap-Binding Complex YHR086W [NAM8] RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of MER2 pre-mRNA RNA-binding protein|U1 snRNP protein Null mutant is viable; defective in meiotic recombination, formation of viable spores, and formation of meiosis-specific double-strand breaks and crossover and noncrossover recombinants; overexpression suppresses mitochondrial splicing defects; impaired association of yeast-specific U1 snRNP proteins but hyperstabilized association of Snu65p/Prp42p with the U1 snRNP; affects in vivo splicing of introns with non-canonical 5'-splice sites; mutant contains a U1 snRNP with aberrant migration behaviour on native gels YIL061C [SNP1] Component of U1 snRNP required for mRNA splicing via spliceosome; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein U1snRNP 70K protein homolog Null mutant is inviable in some strain backgrounds and in other strain backgrounds, null mutant is viable, exhibits greatly increased doubling rates, severe temperature sensitivities, and defects in nuclear pre-mRNA splicing YGR013W [SNU71] Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart U1 snRNP protein YDR240C [SNU56] Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex U1 snRNP protein Null mutant is inviable; mutation affects the in vitro formation of commitment complexes and spliceosomes and the in vivo splicing efficiency of certain introns.

YBR119W
[MUD1] U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing U1 snRNP A protein YKL012W [PRP40] U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex U1 snRNP protein Null mutant is inviable; temperature-sensitive mutants show a splicing defect YLR298C [YHC1] Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site YDR473C [PRP3] Splicing factor, component of the U4/U6-U5 snRNP complex snRNP from U4/U6 and U5 snRNPs RNA synthesis defective

YJL203W
[PRP21] Subunit of the SF3a splicing factor complex, required for spliceosome assembly RNA splicing factor Null mutant is inviable, certain prp21 mutations are allele-specific suppressors of prp9 mutations YDL209C [CWC2] Protein involved in pre-mRNA splicing, component of a complex containing Cef1p; interacts with Prp19p; contains an RNA recognition motif; has similarity to S. pombe Cwf2p Null: required for pre-mRNA splicing YPL151C [PRP46] Splicing factor that is found in the Cef1p subcomplex of the spliceosome pre-mRNA splicing factor

YPR101W
[SNT309] Component of NineTeen complex (NTC) containing Prp19p involved in mRNA splicing, interacts physically and genetically with Prp19p Null mutant is viable, temperature sensitive, exhibits defects in splicing at elevated temperature; snt309 prp19 mutants are synthetically lethal

YML049C
[RSE1] Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport An uncharacterized mutant allele grows slowly and exhibits defects in ER-to-Golgi transport and mRNA splicing.

YDL030W
[PRP9] Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex RNA splicing factor YMR240C [CUS1] Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p U2 snRNP protein suppresses cold sensitivity of a U2 G53A cs mutant YMR213W [CEF1] Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p protein complex component associated with the splicing factor Prp19p Null mutant is inviable, arrests in G2/M, exhibits abnormal nuclear morphologies. Essential for mRNA splicing.

YLR117C
[CLF1] Essential splicesome assembly factor; contains multiple tetratricopeptide repeat (TPR) protein-binding motifs and interacts specifically with many spliceosome components, may serve as a scaffold during splicesome assembly pre-mRNA splicing factor Null mutant is inviable; clf1 alleles show synthetic lethality with cdc40/prp17 and are defective in 5' splice site cleavage [URK1] Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP uridine kinase YHL018W YER023W [PRO3] Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis delta 1-pyrroline-5-carboxylate reductase proline requiring YBR176W [ECM31] Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2dehydropantoate YLR245C [CDD1] Cytidine deaminase; catalyzes the modification of cytidine to uridine in vitro but native RNA substrates have not been identified, localizes to both the nucleus and cytoplasm cytidine deaminase YPR193C [HPA2] Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity histone acetyltransferase Null mutant is viable and does not show any detectable phenotype YJL218W YDR321W [ASP1] Cytosolic L-asparaginase, involved in asparagine catabolism asparaginase I Aspartic acid requiring YEL066W [HPA3] D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro D-Amino acid Nacetyltransferase Null mutant is viable and does not show any detectable phenotype YNL331C [AAD14] Putative aryl-alcohol dehydrogenase with similarity to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role aryl-alcohol dehydrogenase (putative)

YBR252W
[DUT1] dUTPase, catalyzes the hydrolysis of dUTP to dUMP and PPi and thereby prevents the incorporation of uracil into DNA during replication dUTP pyrophosphatase YML064C [TEM1] GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis GTP-binding protein|ras family Null mutant is inviable; net1-1 can suppress the lethality of a tem1 deletion by enabling Clb2p degradation and Sic1p accumulation; tem1-3 temperature sensitive mutants arrest in late anaphase with large buds, an elongated spindle and separated DNA; overexpression of CDC15, CDC5, SIC1, SPO12, and CDC14 can suppress the ts growth defects of tem1-3; overexpression of CLB2 is toxic to tem1-3 mutants at permissive temperature; deletion of cfi1 suppresss the temperature sensitivity of tem1-1 mutants [ROM2] GDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEP Null mutant is viable but shows temperature-and cold-sensitive growth defects at 37 and 11 degrees, increased sensitivity to benomyl, and elongated buds and abnormal mating projections at the permissive temperature; synthetically lethal with rom1 YBR011C [IPP1] Cytoplasmic inorganic pyrophosphatase (PPase), catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase inorganic pyrophosphatase YLR425W [TUS1] Guanine nucleotide exchange factor (GEF) that functions to modulate Rhop1 activity as part of the cell integrity signaling pathway; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Null mutant is viable; shows temperature sensitive growth above 37 degrees C, but no detectable secretory or endocytosis defect. [RLI1] Essential iron-sulfur protein required for ribosome biogenesis and translation initiation; facilitates binding of a multifactor complex (MFC) of translation initiation factors to the small ribosomal subunit; predicted ABC family ATPase Null mutant is inviable; overexpression of RLI1 from a galactose-inducible promoter has a moderate inhibitory effect on growth. [FKH1] Transcription factor of the forkhead family that regulates the cell cycle and pseudohyphal growth; also involved in chromatin silencing at HML and HMR forkhead protein YER025W [GCD11] Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met translational initiation factor eIF-2 gamma subunit Null mutant is inviable, gcd11 mutants have slower growth rate under nonstarvation conditions YNL265C [IST1] Putative translation initiation factor, as suggested by computational analysis of large-scale protein-protein interaction data YJR007W [SUI2] Alpha subunit of the translation initiation factor eIF2, involved in the identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP Translation initiation factor eIF-2 alpha subunit suppression of initiator codon mutations YPL237W [SUI3] Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding translation initiation factor eIF-2 beta subunit suppression of initiator codon mutations YLR291C [GCD7] Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression negative regulator of GCN4 expression|translation initiation factor eIF2B subunit Null mutant is inviable; non-null mutants exhibit an increase in GCN4 translation

YDR211W
[GCD6] Catalytic epsilon subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression translation initiation factor eIF-2B epsilon subunit Null mutant is inviable; non-null mutations increase GCN4 translation YGR083C [GCD2] Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression 71 kDa subunit (delta)|translation initiation factor eIF2B subunit|translational repressor of GCN4 protein Null mutant is inviable; resistance to 5-methyltryptophan, 5-fluorotryptophan and canavanine; override requirement for GCN2 and GCN3 gene products for derepression of GCN4 constitutive derepression and slow growth; temperature sensitive for growth YKR026C [GCN3] Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression eIF2B 34 kDa alpha subunit null mutants fail to derepress amino acid-regulated genes under conditions of amino acid starvation YOR260W [GCD1] Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression gamma subunit|negative regulator in the general control of amino acid biosynthesis|translation initiation factor eIF2B subunit affect growth rate under nonstarvation conditions

YNL062C
[GCD10] Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression RNA-binding protein|subunit of tRNA(1methyladenosine) methyltransferase, along with Gcd14p Null mutant is inviable. There are mutants available that show constitutive HIS4 transcription and slow growth YOL087C

YNL244C
[SUI1] Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase translation initiation factor eIF1 YPR041W [TIF5] Translation initiation factor eIF-5; N-terminal domain functions as a GTPase-activating protein to mediate hydrolysis of ribosomebound GTP; C-terminal domain is the core of ribosomal preinitiation complex formation Translation initiation factor eIF5

YOR361C
[PRT1] Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes translation initiation factor eIF3 subunit YLR192C [HCR1] Dual function protein involved in translation initiation as a substoichiometric component of eukaryotic translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Substoichiometric component of eukaryotic translation initiation factor 3 (eIF3)

YBR079C
[RPG1] Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes translation initiation factor eIF3 subunit Null mutant is inviable; temperature sensitive mutant arrests in G1 phase

YMR146C
[TIF34] Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation translation initiation factor eIF3 subunit YDR429C [TIF35] Subunit of the core complex of translation initiation factor 3(eIF3), which is essential for translation translation initiation factor eIF3 subunit

YMR309C
[NIP1] Subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection translation initiation factor eIF3 subunit Null mutant is inviable; nip1-1 is a temperature-sensitive mutant defective in nuclear transport [SUP35] Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype translation termination factor eRF3 accumulation of large budded cells and substantial arrest of DNA synthesis at the nonpermissive temperature (arrests at G(sub)1/S transition); omnipotent suppressor of nonsense mutations

YMR080C
[NAM7] ATP-dependent RNA helicase of the SFI superfamily, required for nonsense mediated mRNA decay and for efficient translation termination at nonsense codons; involved in telomere maintenance helicase (putative) Null mutant is viable, exhibits impairment in respiratory growth that is exacerbated by low temperatures; exhibits stabilization of nonsense-containing mRNAs which leads to a nonsense suppression phenotype

YGR072W
[UPF3] Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Null mutant is viable but shows increased accumulation of mRNA containing a premature stop codon due to mRNA stabilization

YBR143C
[SUP45] Polypeptide release factor involved in translation termination; mutant form acts as a recessive omnipotent suppressor eRF1 (eukaryotic Release Factor 1) homolog The null mutant is inviable. Other mutant alleles produce a variety of phenotypes which can include: omnipotent nonsense suppression, osmotic sensitivity, benomyl sensitivity, paromomycin sensitivity, and novobiocin resistance.

YML068W
[ITT1] Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins YAR042W [SWH1] Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction YDL019C [OSH2] Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability YER120W [SCS2] Integral ER membrane protein that regulates phospholipid metabolism via an interaction with the FFAT motif of Opi1p, also involved in telomeric silencing, disruption causes inositol auxotrophy above 34 degrees C, VAP homolog [RRP8] Nucleolar protein involved in rRNA processing, pre-rRNA cleavage at site A2; also involved in telomere maintenance; mutation is synthetically lethal with a gar1 mutation nucleolar protein required for efficient processing of pre-rRNA at site A2; methyltransferase homolog YNR024W YHR081W [LRP1] Substrate-specific nuclear cofactor for exosome activity in the processing of stable RNAs; required for telomere length maintenance; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination

YOL021C
[DIS3] Nucleolar exosome component, involved in rRNA processing and RNA degradation, binds Gsp1p/Ran and enhances the GEF activity of Srm1p, implicated in mitotic control, homologous to the E. coli RNase R of the RNase II family 3'-5' exoribonuclease complex subunit YNL232W [CSL4] Subunit of the exosome, which is an essential complex present in both nucleus and cytoplasm that mediates RNA processing and degradation Null mutant is inviable, csl4-1 exhibits double mutant inviability in combination with cbf1(cep1) deletion mutants YOR001W [RRP6] Exonuclease component of the nuclear exosome; contributes to the quality-control system that retains and degrades aberrant mRNAs in the nucleus Null mutant is viable, heat sensitive; other mutants show a 5.8S rRNA 3' end formation defect

YCR035C
[ [NPL3] RNA-binding protein that carries poly(A)+ mRNA from the nucleus into the cytoplasm; phosphorylation by Sky1p in the cytoplasm may promote release of mRNAs contains RNA recognition motif|nuclear shuttling protein Null mutant is inviable, npl3 mutants are temperature-sensitive for growth, but do not exhibit a defect in localization of nuclear proteins

YOL123W
[HRP1] Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRMcontaining heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences cleavage and polyadenylation factor CF I component involved in pre-mRNA 3'-end processing Null mutant is inviable; mutants can suppress temperaturesensitive alleles of npl3 (but not npl3 null mutants)

YBR034C
[HMT1] Nuclear SAM-dependent mono-and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, thus facilitating nuclear export of these proteins; required for viability of npl3 mutants arginine methyltransferase|mono-and asymmetrically dimethylating enzyme Null mutant is viable, hmt1 npl3-1 mutants are inviable YGL122C [NAB2] Nuclear polyadenylated RNA-binding protein; autoregulates mRNA levels; related to human hnRNPs; has nuclear localization signal sequence that binds to Kap104p; required for poly(A) tail length control and nuclear mRNA export polyadenylated RNA binding protein

YKL139W
[CTK1] Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing kinase subunit of RNA polymerase II carboxy-terminal domain kinase I Null mutations in each of the CTK1, CTK2, and CTK3 genes cause slow growth, cold-sensitivity, flocculence, and enlarged cell size.

YJL006C
[CTK2] Beta subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing; has similarity to cyclins RNA polymerase II C-terminal domain kinase beta subunit, similar to cyclin Null mutations in each of the CTK1, CTK2, and CTK3 genes cause slow growth, cold-sensitivity, flocculence, and enlarged cell size.

YML112W
[CTK3] Gamma subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing RNA polymerase II C-terminal domain kinase gamma subunit, similar to cyclin-dependent kinase Null mutations in each of the CTK1, CTK2, and CTK3 genes cause slow growth, cold-sensitivity, flocculence, and enlarged cell size.

YPL127C
[HHO1] Histone H1, a linker histone required for nucleosome packaging at restricted sites; suppresses DNA repair involving homologous recombination; not required for telomeric silencing, basal transcriptional repression, or efficient sporulation histone H1 Null mutant is viable; other phenotype: Increased basal expression of a CYC1-lacz reporter gene; nuclear localization of a Hho1-GFP fusion protein YBR020W [GAL1] Galactokinase, phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p galactokinase Null mutant is viable and cannot utilize galactose.

YOR191W
[RIS1] Member of the SWI/SNF family of DNA-dependent ATPases, plays a role in antagonizing silencing during mating-type switching, contains an N-terminal domain that interacts with Sir4p and a C-terminal SNF2 domain SWI2/SNF2 DNA-dependent ATPase family member (putative) Null mutant is viable but shows slower mating type switching; interferes with silencing when overexpressed YDR138W [HPR1] Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Increased intrachromosomal recombination YCL011C [GBP2] Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Hrb1p and Npl3p; also binds single-stranded telomeric repeat sequence in vitro contains RNA recognition motifs Mutation alters the distribution of Rap1p, a telomere-associated protein, but has no effect on telomere length or telomere position YNL004W [HRB1] Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Gbp2p and Npl3p hypothetical RNA-binding protein YDL084W [SUB2] Component of the TREX complex required for nuclear mRNA export; DEAD-box RNA helicase involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56 ATP-dependent RNA helicase Null mutant is inviable; sub2 allele suppresses cold-sensitive snRNP phenotype of brr1-1 YNL139C [RLR1] Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongationassociated recombination; required for LacZ RNA expression from certain plasmids Null mutant is viable but shows poor growth and a temperature-sensitive phenotype.Increased frequencies of recombination between direct repeats (>1000-fold above wild-type levels) that is linked to transcriptional elongation defects. General defects in RNA polII transcription. Incapacity to transcribe lacZ. Overexpression of RLR1 suppresses the ts phenotype and the incapacity to transcribe lacZ sequences of hpr1-delta mutants YNL253W [TEX1] Protein involved in mRNA export, component of the transcription export (TREX) complex YHR167W [THP2] Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance null mutant is viable, hyperrecombination between direct repeats dependent on transcription elongation, transcription elongation impairment, inability to properly transcribe lacZ sequences YML062C [MFT1] Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance mitochondrial targeting protein

YOR253W
[NAT5] Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing N-acetyltransferase [STM1] Protein that binds G4 quadruplex and purine motif triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts with ribosomes and subtelomeric Y' DNA; multicopy suppressor of tom1 and pop2 mutations purine motif triplex-binding protein Null mutant is viable; overexpression of STM1 suppresses some phenotypes of pop2 null mutations and the temperature sensitivity of tom1 and htr1 mutants. Cells lacking Stm1 display deficiency in the apoptosis-like cell death process induced by treatment with low concentrations of H2O2. Survival is increased when Stm1 is completely missing from the cells or when inhibition of Stm1 synthesis permits proteasomal degradation to decrease its amount in the cell. Stm1 accumulation induces cell death. [NOC3] Protein that forms a nuclear complex with Noc2p that binds to 66S ribosomal precursors to mediate their intranuclear transport; also binds to chromatin to promote the association of DNA replication factors and replication initiation YNR053C

YGL189C
[NOG2] Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation part of a pre-60S complex YPR143W [RRP15] Nucleolar protein, constituent of pre-60S ribosomal particles; required for processing of the 27S pre-rRNA at the A2 site to yield 5.8S and 25S rRNA YGR245C [SDA1] Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis YLR074C [BUD20] Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern YHR197W [RIX1] Essential protein involved in the processing of the ITS2 region of the rRNA locus; required for the maturation and nuclear export of the 60S ribosomal subunit YKL014C [URB1] Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit YKL009W [MRT4] Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Null mutant exhibits slow growth. ts mutation results in decreased decay rates of mRNAs [RRP1] Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles Null mutant is inviable, cannot be suppressed by srd1 mutations. rrp1-1 mutations are associated with temperature-sensitive growth, a conditional defect in processing of 27S pre-rRNA to mature 25S rRNA, and a nonconditional increase in sensitivity to several aminoglycoside antibiotics. srd1 is an allele-specific suppressor of rrp1-1.

YOL077C
[BRX1] Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif YOR206W [NOC2] Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors YMR049C [ERB1] Protein required for maturation of the 25S and 5.8S ribosomal RNAs; constituent of 66S pre-ribosomal particles; homologous to mammalian Bop1 YKR081C [RPF2] Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles YNL061W [NOP2] Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles 90 kDa protein homologous to a human proliferationassociated nucleolar protein, p120 Null mutant is inviable; overexpression leads to changes in nucleolar morphology YOR272W [YTM1] Constituent of 66S pre-ribosomal particles, required for maturation of the large ribosomal subunit microtubule-associated protein YPR016C [TIF6] Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Null mutant is inviable; cells are depleted of 60S ribosomal subunits, translation initiation is inhibited, and cells arrest in G1

YGR103W
[NOP7] Nucleolar protein involved in rRNA processing and 60S ribosomal subunit biogenesis; constituent of several different pre-ribosomal particles YHR052W [CIC1] Essential protein that interacts with proteasome components and has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles Null: lethal. Other phenotypes: cic1-2 ts mutant stabilizes F-box proteins. [NSR1] Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis nuclear localization sequence binding protein Null mutant is viable, shows severe growth defect.

YDR398W
[UTP5] Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA U3 snoRNP protein YLR175W [CBF5] Component of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), probable rRNA pseudouridine synthase, binds to snoRNP Nop10p and also interacts with ribosomal biogenesis protein Nop53p major low affinity 55 kDa centromere/microtubule binding protein YPL012W [RRP12] Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; contains HEATrepeats YNL075W [IMP4] Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs U3 snoRNP protein YLR197W [SIK1] Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects U3 snoRNP protein wild-type gene suppresses toxicity of GAL4-I-Kappa-B alpha in yeast|Other phenotypes: Shortens the G1 phase of the cell cycle when present in high-copy YNL308C [KRI1] Essential nucleolar protein required for 40S ribosome biogenesis; physically and functionally interacts with Krr1p Krr1p binding protein YDR064W [RPS13] Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ribosomal proteins ribosomal protein S13 (S27a) (YS15) YDL213C [NOP6] Protein with similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; computational analysis of large-scale protein-protein interaction data suggests a possible role in rRNA processing [SOF1] Essential subunit of the U3 (box C+D) snRNP complex required for 2' O-methylation of pre-RNA; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p U3 snoRNP protein Null mutant is inviable. sof1-56, a dominant suppressor of nop1 mutants can restore growth and pre-RNA processing at 35 degrees C. In vivo depletion of SOF1 leads to impaired pre-rRNA processing and inhibition of 18S rRNA production.

YDL014W
[NOP1] Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin U3 snoRNP protein|similar to mammalian fibrillarin Null mutant is inviable. Temperature-sensitive alleles exhibit various defects in rRNA processing.|

YLR186W
[EMG1] Protein required for the maturation of the 18S rRNA and for 40S ribosome production; associated with spindle/microtubules; nuclear localization depends on physical interaction with Nop14p; may bind snoRNAs ribosome biogenesis YLR129W [DIP2] Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex U3 snoRNP protein YLR409C [UTP21] Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale proteinprotein interaction data U3 snoRNP protein

YLR222C
[UTP13] Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA U3 snoRNP protein YHR148W [IMP3] Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA U3 snoRNP protein Null mutant is inviable. Depletion of Imp3p prevents the synthesis of mature 18S rRNA.

YJL069C
[UTP18] Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale proteinprotein interaction data U3 snoRNA associated protein|U3 snoRNP protein Null: lethal. Other phenotypes: required for 18S RNA production YMR229C [RRP5] Protein required for the synthesis of both 18S and 5.8S rRNA; C-terminal region is crucial for the formation of 18S rRNA and Nterminal region is required for the 5.8S rRNA; component of small ribosomal subunit (SSU) processosome U3 snoRNP protein Overexpression of RRP5 facilitates mitochondrial import of hydrophobic proteins; overexpression of an RRP5 mutant complements the rRNA processing defect of the null alllele, but does not facilitate mitochondrial import; required for processing of pre-rRNA YBL004W [UTP20] Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA U3 snoRNP protein YBR247C [ENP1] Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus 57 kDa protein with an apparent MW of 70 kDa by SDS-PAGE (putative)

YDL148C
[NOP14] Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA U3 snoRNP protein YOR310C [NOP58] Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA U3 snoRNP protein Null mutant is inviable; in vivo depletion impairs synthesis of the 40S ribosomal subunit

YGR128C
[UTP8] Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA U3 snoRNP protein [HOS4] Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate YDR155C [CPR1] Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A cyclophilin|peptidyl-prolyl cis-trans isomerase (PPIase)

YKR029C
[SET3] Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains [PUP1] Endopeptidase with trypsin-like activity that cleaves after basic residues; beta-type subunit of 20S proteasome synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z proteasome subunit (putative) [ECM29] Major component of the proteasome; tethers the proteasome core particle to the regulatory particle, and enhances the stability of the proteasome YDL147W [RPN5] Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p proteasome regulatory particle subunit YFR010W [UBP6] Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds

YHR027C
[RPN1] Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein?protein interactions 26S proteasome PA700 subunit Null mutant is inviable; hrd2-1 mutation slows degradation of Hmg2p. hrd2-1 strains are sensitive to canavanine and show a global accumulation of ubiquitin-conjugated proteins, but are not temperature-sensitive

YFR004W
[RPN11] Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates YER021W [RPN3] Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control 26S proteasome regulatory module component Null mutant is inviable. RPN3 is a high copy suppressor of the nin1-1 temperature sensitive phenotype

YKL145W
[RPT1] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p 26S protease subunit component (putative)|ATPase

YDR394W
[RPT3] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Null mutant is inviable; yta2 is an extragenic suppressor of yme1 mutations YDR363W-A [SEM1] Component of the lid subcomplex of the regulatory subunit of the 26S proteasome; ortholog of human DSS1 Null mutant is viable but is temperature-sensitive in a sigma1278b background (but not in a S288C background). The null mutation suppresses the temperature sensitivity of sec3-2, sec8-9, sec10-2 and sec15-1.

YHR200W
[RPN10] Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein 26S proteasome component|mammalian S5a protein homolog Null mutant is viable, exhibits a modest sensitivity to amino acid analogs and has increased steady-state levels of ubiquitin-protein conjugates

YFR052W
[RPN12] Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p 32-34 kDa protein Null mutant is inviable; nin1-1 mutant is temperature-sensitive mutant that shows i) higher rates of recombination and chromosome and plasmid loss; ii) greater sensitivity to UV irradiation; iii) at restrictive temperature, arrest in G2, failure to activate histone H1 kinase, and accumulation of polyubiquinated proteins

YGL048C
[RPT6] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle ATPase

YIL075C
[RPN2] Subunit of the 26S proteasome, substrate of the N-acetyltransferase Nat1p Null mutant is inviable/null mutant is viable, but shows temperature sensitivity (conflicting reports)

YDR427W
[RPN9] Non-ATPase regulatory subunit of the 26S proteasome, has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects proteasome regulatory particle subunit Null mutant is viable, temperature sensitive; rpn9 rpn10 double deletion mutants are viable

YOR261C
[RPN8] Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p proteasome regulatory particle subunit

YOR117W
[RPT5] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription

YDL097C
[RPN6] Essential, non-ATPase regulatory subunit of the 26S proteasome lid required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion proteasome regulatory particle subunit

YOR259C
[RPT4] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for spindle pole body duplication; localized mainly to the nucleus throughout the cell cycle 26S proteasome cap subunit component|ATPase Null mutant is inviable; ts mutant strain arrests as large-budded cells after 1, 2, 3 divisions with a G2 content of DNA and a monopolar spindle; unduplicated spindle pole body is enlarged as in other monopolar mutants; they also fail to arrest at G1 when starved for a single amino acid (but do arrest at G1 when deprived of all nitrogen), are resistant to cyclohexamide, and are hypersensitive to amino acid analogs, hygromycin B and 3-aminotriazole

YDL007W
[RPT2] One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle one of the ATPase subunits of the proteasome YLR421C [RPN13] Subunit of the 19S regulatory particle of the 26S proteasome lid Null mutant is viable but defective in degradation of ubiquitinated substrates.

YPR108W
[RPN7] Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits proteasome regulatory particle subunit [SPO74] Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation YJL039C [NUP192] Essential structural subunit of the nuclear pore complex (NPC), localizes to the nuclear periphery of nuclear pores, homologous to human p205 nuclear pore complex subunit YMR153W [NUP53] Subunit of the nuclear pore complex (NPC), interacts with karyopherin Kap121p or with Nup170p via overlapping regions of Nup53p, involved in activation of the spindle checkpoint mediated by the Mad1p-Mad2p complex karyopherin docking complex component of the nuclear pore complex|nuclear pore complex subunit Null mutant is viable but disrupts Kap121 localization to the nuclear envelope.

YDL088C
[ASM4] Nuclear pore complex subunit, part of a subcomplex also containing Nup53p, Nup170p, and Pse1p nuclear pore complex subunit Null mutant is viable in some strain backgrounds (including CEN.PK2); however, in the FY1679 genetic background, it is inviable. [NUP159] Subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, forms a subcomplex with Nup82p and Nsp1p, required for mRNA export nucleoporin Null mutant is inviable; at nonpermissive temperature, a temperature-sensitive mutant shows cessation of mRNA export without cytoplasmic accumulation of NLS-containing reporter protein, while at permissive temperature, the nuclear pore complexes are clustered; temperature-sensitive allele is synthetically lethal with nup120 and is suppressed by high copy GLE1

YJL061W
[NUP82] Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup159p and Nsp1p, interacts with Nup116p and is required for proper localization of Nup116p in the NPC 82 kDa protein, with putative coiled-coil domain, has carboxy-terminal domain, containing heptad repeats, that binds Nsp1p|nuclear pore complex subunit|nucleoporin Null mutant is inviable; cells depleted of Nup82p, or cells with temperature-sensitive Nup82p at nonpermissive temperature, show defect in poly(A)+RNA export but no major alterations in nuclear envelope structure or nuclear pore density YNL189W [SRP1] Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation karyopherin alpha supressor of rpb1, cold-sensitive YBR017C [KAP104] Transportin, cytosolic karyopherin beta 2 involved in delivery of heterogeneous nuclear ribonucleoproteins to the nucleoplasm, binds rg-nuclear localization signals on Nab2p and Hrp1p, plays a role in cell-cycle progression karyopherin beta 2 Null mutant is viable at 23 degrees C, but fails to germinate and dies at 30 C, shows severe nuclear envelope defects YAR002W [NUP60] Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate; involved in telomere maintenance nuclear pore complex subunit YER110C [KAP123] Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 karyopherin beta 4

YOR160W
[MTR10] Nuclear import receptor, mediates the nuclear localization of proteins involved in mRNA-nucleus export YLR347C [KAP95] Karyopherin beta, forms a dimeric complex with Srp1p (Kap60p) that mediates nuclear import of cargo proteins via a nuclear localization signal (NLS), interacts with nucleoporins to guide transport across the nuclear pore complex karyopherin beta (importin 90) homolog (95 kDa) essential, ts mutant shows nuclear import defect YJL041W [NSP1] Essential component of the nuclear pore complex, which mediates nuclear import and export nuclear pore complex subunit YBL079W [NUP170] Abundant subunit of the nuclear pore complex (NPC), required for proper localization of specific nucleoporins within the NPC, involved in nuclear envelope permeability and in chromosome segregation, has similarity to Nup157p nuclear pore complex subunit Null mutant is viable; synthetically lethal with nup157, nup188, and pom152; changing NUP170 expression causes morphological abnormalities in nuclear envelope

YMR308C
[PSE1] Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p karyopherin Null mutant is viable but grows very slowly; overexpression of PSE1 results in enhanced protein secretion YLR335W [NUP2] Protein involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization nucleoporin Null mutant is viable; some combinations of alleles of nup1, nsp1 and nup2 are synthetically lethal YML103C [NUP188] Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, also involved in nuclear envelope permeability, interacts with Pom152p and Nic96p nuclear pore complex subunit Null mutant is viable but exhibits abnormalities in nuclear envelope and nuclear pore morphology; dominant mutants of nup188 are temperature-sensitive and show nuclear envelope herniations; synthetically lethal with pom152, nup157, and nup170 YFR002W [NIC96] Component of the nuclear pore complex, required for nuclear pore formation; forms a subcomplex with Nsp1p, Nup57p, and Nup49p 96 kDa nucleoporin-interacting component|nuclear pore complex subunit YDR192C [NUP42] Subunit of the nuclear pore complex (NPC) that localizes exclusively to the cytoplasmic side; involved in RNA export, most likely at a terminal step; interacts with Gle1p 42 kDa protein associated with nuclear pore complexes; structurally related to the FG-nucleoporin family of pore proteins|nuclear pore complex subunit Null mutant is viable, NUP42 is essential for the export of heat shock mRNAs following stress

YLR208W
[SEC13] Component of both the Nup84 nuclear pore sub-complex and of the COPII complex (Sar1p, Sec13p, Sec16p, Sec23p, Sec24p, Sec31p, Sfb2p, and Sfb3p) which is important for the formation of ER to Golgi transport vesicles nuclear pore complex subunit|protein involved in release of transport vesicles from the ER Null mutant is inviable; ts mutants exhibit defects in secretion.

YDR335W
[MSN5] Karyopherin involved in nuclear import and export; shown to be responsible for nuclear import of replication protein A and for export of several proteins including Swi6p, Far1p, and Pho4p; cargo dissociation involves binding to RanGTP Disruptants are not completely sterile YKL068W [NUP100] Subunit of the nuclear pore complex (NPC) that is localized to both sides of the pore; contains a repetitive GLFG motif that interacts with mRNA export factor Mex67p and with karyopherin Kap95p; homologous to Nup116p nuclear pore complex subunit Null mutant is viable with no obvious phenotypes; synthetically lethal with nup116 and gle2 mutants YGR119C [NUP57] Essential subunit of the nuclear pore complex (NPC), functions as the organizing center of an NPC subcomplex containing Nsp1p, Nup49p, Nup57p, and Nic96p nucleoporin YGL172W [NUP49] Subunit of the Nsp1p-Nup57p-Nup49p-Nic96p subcomplex of the nuclear pore complex (NPC), required for nuclear export of ribosomes nuclear pore complex subunit Null mutant is inviable; some nsp1 nsp49 alleles exhibit synthetic lethality YMR047C [NUP116] Subunit of the nuclear pore complex (NPC) that is localized to both sides of the pore; contains a repetitive GLFG motif that interacts with mRNA export factor Mex67p and with karyopherin Kap95p; homologous to Nup100p nuclear pore complex subunit Null mutant grows slowly, accumulates unspliced pre-tRNAs, acumulates poly(A)+ RNA in the nucleus, and is temperature-sensitive; at nonpermissive temperature, null mutants show membrane seals covering cytoplasmic face of nuclear pore complexes; synthetically lethal with nsp1, nup100, and nup145

YGL100W
[SEH1] Nuclear pore protein that is part of the evolutionarily conserved Nup84p complex (Nup84p, Nup85p, Nup120p, Nup145p, and Seh1p); homologous to Sec13p nuclear pore complex subunit YER105C [NUP157] Abundant subunit of the nuclear pore complex (NPC), present on both sides of the NPC, has similarity to Nup170p nuclear pore complex subunit Null mutant is viable; synthetically lethal with nup170 and nup188

YGL092W
[NUP145] Essential nucleoporin, catalyzes its own cleavage in vivo to generate a C-terminal fragment that assembles into the Nup84p subcomplex of the nuclear pore complex, and an N-terminal fragment of unknown function that is homologous to Nup100p nuclear pore complex subunit Null mutant is inviable, depletion of Nup145p in vivo leads rapidly to nuclear retention of polyadenylated RNAs and more slowly to cytoplasmic accumulation of a nuclear reporter protein YKR082W [NUP133] Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), localizes to both sides of the NPC, required to establish a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p nuclear pore complex subunit Null mutant is viable but grows slowly and is temperature-sensitive; at nonpermissive temperature, poly(A)+ RNA accumulates in nucleus (although nuclear import of karyophilic proteins is not blocked) and nuclear pores cluster; synthetically lethal with nup120

YJR042W
[NUP85] Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking nuclear pore complex subunit Null mutant is viable but is temperature-sensitive; at nonpermissive temperature, null mutant accumulates poly(A)+ RNA and has fragmented nucleolus; at permissive temperature, nuclear envelope of null mutant detaches from nucleus

YDL116W
[NUP84] Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup85p, Nup120p, Nup145p-C, Sec13p, and Seh1p that plays a role in nuclear mRNA export and NPC biogenesis nuclear pore complex subunit|similar to mammalian Nup107p Null mutant is viable but has defects in nuclear membrane and nuclear pore complex organization and in poly(A)+ RNA transport

YKL057C
[NUP120] Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for even distribution of NPCs around the nuclear envelope, involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p 100 kDa protein (predicted molecular weight is 120 kDa) with two leucine zipper motifs, coiled-coil region, and some homology to Nup133p|nuclear pore complex subunit Null mutant is viable but grows slower, is temperature-sensitive, and shows nucleolar fragmentation and clustering of nuclear pore complexes; at nonpermissive temperature, null mutant accumulates poly(A)+ mRNA in nucleus and shows nucleolar fragmentation and spindle defects; temperature sensitivity can be suppressed by growth in high osmolarity media; synthetically lethal with nup133 and nup159

YMR059W
[SEN15] Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p tetrameric tRNA splicing endonuclease 15kDa subunit YAR008W [SEN34] Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease tetrameric tRNA splicing endonuclease 34 kDa subunit Null mutant is inviable and shows H242A impaired 3'splice site cleavage

YMR205C
[PFK2] Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes phosphofructokinase beta subunit Null mutant is viable but exhibits slow growth and decreased efficiency of glucose utilization. [ERG1] Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine squalene monooxygenase Null mutant is inviable when cells are grown under aerobic conditions; erg1 null mutants are viable under anaerobic conditions during which ergosterol is taken up by the cells YNL272C [SEC2] Guanyl-nucleotide exchange factor for the small G-protein Sec4p, located on cytoplasmic vesicles; essential for post-Golgi vesicle transport GDP/GTP exchange factor accumulates secretory vesicles

YDR004W
[RAD57] Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p RecA homolog|interacts with Rad 55p by two-hybrid analysis|similar to DMC1, RAD51, and RAD55 Null mutant is viable, radiation sensitive|Deletion of this homologous recombination (HR) gene decreases psoralen-induced recombination and increases mutation frequencies.

YDR076W
[RAD55] Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p RecA homolog|interacts with Rad51p and Rad57p by two-hybrid analysis|similar to DMC1, RAD51, RAD57 Null mutant is viable, radiation sensitive, x-ray sensitive|Deletion of this homologous recombination (HR) gene decreases psoralen-induced recombination and increases mutation frequencies.

YHR154W
[RTT107] Protein that interacts with Mms22p and is implicated in Mms22-dependent DNA repair during S phase, damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; has four BRCT domains; has a role in regulation of Ty1 [YEF3] Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP Translation elongation factor 3 (EF-3)

YDR234W
[LYS4] Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway homoaconitase Lysine requiring YPR080W [TEF1] Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes translational elongation factor EF-1 alpha YKL056C [TMA19] Protein of unknown function that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YKL056C is not an essential gene

YAL003W
[EFB1] Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site translation elongation factor EF-1beta

YPL048W
[CAM1] Translational cofactor elongation factor-1 gamma, participates in the regulation of GTP-binding protein EF-1 alpha, may play a redundant role in the regulation of protein synthesis or another GTP-dependent process calcium and phospholipid binding protein homologous to translation elongation factor 1-gamma (EF-1gamma) 0313 GO_TERM:[sphingolipid biosynthesis] P-Value:5.3e-06

YEL022W
[GEA2] Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea1p ARF GTP/GDP exchange factor Null mutant is viable, synthetically lethal with gea1 null mutant YER100W [UBC6] Ubiquitin-conjugating enzyme involved in ER-associated protein degradation; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway ubiquitin-conjugating enzyme [HSP42] Small cytosolic stress-induced chaperone that forms barrel-shaped oligomers and suppresses the aggregation of non-native proteins; oligomer dissociation is not required for function; involved in cytoskeleton reorganization after heat shock Null mutant is viable; hsp42 hsp26 double deletion mutants are viable; hsp42 null mutants subjected to moderate thermal stress reorganize the actin cytoskeleton more slowly than wild-type YHR152W [SPO12] Nucleolar protein of unknown function, positive regulator of exit from mitosis; involved in regulating the release of Cdc14p from the nucleolus in early anaphase; proposed to play similar role in meiosis 20 kDa protein with negatively charged C-terminus required for function|positive regulator of exit from M-phase in mitosis and meiosis (putative) sporulation defective; loss of function in mitosis results in delay in G2; loss of function in meiosis results in a prolonged pachytene stage and presence of synaptonemal complexes, a single meiosis IIlike equational division at the time of meiosis II, and dyad asci containing two diploid spores. Gain of function in mitosis suppresses M-phase anaphase arrest caused by overexpression of CLB2 deg-and mutants (e.g. dbf2-ts). mRNA is cell cycle regulated (with DBF2 ) in mitosis and increases 5-10x in meiosis.

YHR208W
[BAT1] Mitochondrial branched-chain amino acid aminotransferase, homolog of murine ECA39; highly expressed during logarithmic phase and repressed during stationary phase branched-chain amino acid transaminase|highly similar to mammalian ECA39, which is regulated by the oncogene myc Null mutant is viable; ILV auxotrophy in bat1 bat2 double mutant YJR148W [BAT2] Cytosolic branched-chain amino acid aminotransferase, homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase branched-chain amino acid transaminase [SLC1] 1-acyl-sn-gylcerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; located in lipid particles and endoplasmic reticulum 1-acyl-sn-gylcerol-3-phosphate acyl transferase (putative) slc1-1 mutant suppresses sphingolipid long chain biosynthetic defect; the mutant also makes novel phosphatidylinositol derivatives and lacks sphingolipids YDR170C [SEC7] Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles guanine nucleotide exchange protein for ARF [UFD4] Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins ubiquitin ligase e3 Null is viable; defective in proteolysis of fusion proteins containing a 'nonremovable' N-terminal ubiquitin moiety YDR214W [AHA1] Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock Hsp90 system cochaperone; Aha1 binds to the middle domain of Hsp90 and improves client protein activation in vivo YIL094C [LYS12] Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate homo-isocitrate dehydrogenase Null mutant is viable but shows decreased growth in the absence of lysine YGL195W [GCN1] Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA translational activator of GCN4 through activation of GCN2 in response to starvation Null mutant is viable and sensitive to 3-aminotriazole

YFL037W
[TUB2] Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules betatubulin null is inviable; conditional mutants show block of mitotic nuclear migration and chromosome segregation and defects in spindle and/or cytoplasmic microtubules at non-permissive conditions; some mutants are benomyl-hypersensitive

YML085C
[TUB1] Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules alpha-tubulin Null mutant is inviable; heterozygous tub1 null diploids are slow growing and sporulate poorly YMR012W [CLU1] eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA-mutant Sometimes copurifies with translation initiation factor eIF3, but apparently not required for translation initiation Null mutant is viable, growth is normal, mitochondrial network is collapsed to one side of the cell YLL040C [VPS13] Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention YFR009W [GCN20] Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA ATP-binding cassette (ABC) family Null mutant is viable and shows impaired derepression of GCN4 translation and reduced levels of eIF-2 alpha phosphorylation YLR153C [ACS2] Acetyl-coA synthetase isoform, required for growth on glucose; expressed under anaerobic conditions acetyl CoA synthetase Null mutant is viable, and grows on ethanol or acetate as sole carbon source, but is unable to grow on glucose as sole carbon source; acs1 acs2 double null mutant is inviable YKL211C [TRP3] Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p anthranilate synthase component II|indole-3-phosphate Null mutant is viable, tryptophan auxotroph

YER095W
[RAD51] Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Rad51p colocalizes to 6 5 spots with Dmc1p prior to synapsis (independently of ZIP1 and DMC1), and interacts with Rad52p and Rad55p; human Rad51p homolog interacts with Brca2 protein which has been implicated in causing breast cancer|RecA homolog Null mutant is viable; accumulates meiosisspecific double strand breaks at a recombination hotspot and reduces the formation of physical recombinants and processed double strand breaks with long heterogeneous tails; rad51 mutants are also defective for X-ray damage repair and gene conversions; rad51 rad27 mutants are inviable.|Deletion of this homologous recombination (HR) gene decreases psoralen-induced recombination and increases mutation frequencies.

YGR234W
[YHB1] Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses flavohemoglobin Null mutant is viable. A rho+ strain carrying a yhb1(-) deletion has normal levels of both cyanide-sensitive and cyanide-insensitive respiration. Cells that carry a yhb1(-) deletion are sensitive to conditions that promote oxidative stress.

YMR311C
[GLC8] Regulatory subunit of protein phosphatase 1 (Glc7p), involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2 protein phosphatase 1 (Glc7p) regulator YOR054C [VHS3] Functionally redundant (see also SIS2) inhibitory subunit of Ppz1p, a PP1c-related ser/thr protein phosphatase Z isoform; synthetically lethal with sis2; putative phosphopantothenoylcysteine decarboxylase involved in coenzyme A biosynthesis YDR436W [PPZ2] Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Null mutant is viable but shows increase in cell size and cell lysis (remediated by 1 M sorbitol); ppz1 ppz2 double mutant shows increased expression of ENA1, resistance to sodium and lithium, and sensitivity to 5 mM caffeine (which is suppressed by 1 M sorbitol)

YML016C
[PPZ1] Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Null mutant is viable, exhibits increased tolerance to Na+ and Li+ cations, increased cell size and lysis; ppz1 ppz2 double deletion mutants exhibit a temperature sensitive cell lysis defect and fail to grow in the presence of 5 mM caffeine 0327 GO_TERM:[negative regulation of ligase activity] P-Value:2.6e-04

YBR050C
[REG2] Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucoserepressible genes, also involved in glucose-induced proteolysis of maltose permease Glc7p regulatory subunit YKR098C [UBP11] Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins ubiquitin-specific protease YGR097W [ASK10] Component of the RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p, which relieves repression of stress-response genes transcriptional activator of the SKN7 mediated 'two-component' regulatory system

YPR030W
[CSR2] Nuclear protein with a potential regulatory role in utilization of galactose and nonfermentable carbon sources; overproduction suppresses the lethality at high temperature of a chs5 spa2 double null mutation; potential Cdc28p substrate YDR017C [KCS1] Inositol hexaphosphate kinase, phosphorylates inositol hexakisphosphate (InsP6) to diphosphoinositol polyphosphates, required for proper vacuole morphology and involved in salt stress response, contains two leucine heptad repeats Inositol polyphosphate kinase Null mutant is viable; kcs1 ptc1 double mutant is inviable; isolated as a suppressor of a hyper-recombination mutant of PKC1

YDR099W
[BMH2] 14-3-3 protein, minor isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle transport, Ras/MAPK signaling during pseudohyphal development, rapamycin-sensitive signaling, and others member of conserved eukaryotic 14-3-3 gene family Null mutant is viable; bmh1 bmh2 double mutant is inviable; (in strain Sigma-1278b, required for pseudohyphal development but not for viability)

YDR130C
[FIN1] Spindle pole body-related intermediate filament protein, forms cell cycle-specific filaments between spindle pole bodies in mother and daughter cells, able to self-assemble, expression induced during S/G2, localization cell-cycle dependent YER177W [BMH1] 14-3-3 protein, major isoform; binds proteins and DNA, involved in regulation of many processes including exocytosis and vesicle transport, Ras/MAPK signaling during pseudohyphal development, rapamycin-sensitive signaling, and others member of conserved eukaryotic 14-3-3 gene family Null mutant is viable; bmh1 bmh2 double mutant is inviable; (in strain Sigma-1278b, required for pseudohyphal development but not for viability) 0328 YMR102C YNL218W [MGS1] Protein with DNA-dependent ATPase and ssDNA annealing activities involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP) mgs1 is synthetic lethal with rad6 and exhibits a synergistic growth defect with rad18 and rad5. mgs1 mutant is not sensitive to DNA-damaging agents, but mgs1 rad5 double mutant has increased sensitivity to hydroxyurea and a greatly increased spontaneous mutation rate. Growth defects of mgs1 rad18 double mutants are suppressed by a mutation in SRS2 or by overexpression of Rad52. mgs1 mutation suppresses temperature sensitivity of POL3 mutants.

YGL208W
[SIP2] One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane

YDR477W
[SNF1] AMP-activated serine/threonine protein kinase found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis serine/threonine kinase Null mutant is viable, sensitive to heat stress and starvation and fails to accumulate glycogen during growth in rich medium; sucrose nonfermenting, high copy MSI1 and PDE2 partially suppress snf1 sporulation defects

YGL115W
[SNF4] Protein kinase activator found in a complex containing Snf1p and members of the Sip1p/Sip2p/Gal83p family; activates the Snf1p protein kinase; involved in expression of glucose-repressed genes, sporulation, and peroxisome biogenesis associates with Snf1p Null mutant is viable, sucrose nonfermenting; high copy MSI1 and PDE2 partially suppress sporulation defect YDR028C [REG1] Regulatory subunit of type 1 protein phosphatase Glc7p, involved in negative regulation of glucose-repressible genes Glc7p regulatory subunit YER027C [GAL83] One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain [HMLALPHA1] Silenced copy of ALPHA1 at HML, encoding a transcriptional coactivator involved in the regulation of mating-type alphaspecific gene expression involved in the regulation of alpha-specific genes|transcription factor YCL067C [HMLALPHA2] Silenced copy of ALPHA2 at HML; homeobox-domain protein that associates with Mcm1p in haploid cells to repress aspecific gene expression and interacts with a1p in diploid cells to repress haploid-specific gene expression

YMR043W
[MCM1] Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes contains the 56 amino-acid MADS (MCM1, AG, DEFAm SRF)-box motif within its DNA binding domain, plays a central role in the formation of both repressor and activator complexes|transcription factor Null mutant is inviable, Pro97Leu mutant is sterile, exhibits defects in minichromosome maintenance

YMR042W
[ARG80] Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p transcription factor Arginine requiring [UME6] Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p C6 zinc finger URS1binding protein Null mutant is viable. Exhibits defects in IME1-dependent activation and repression through URS1 sites. ume6 does not require Mata/Matalpha, starvation, IME1, or IME2 for derepressed expression in mitosis and is epistatic to lethality of IME1 overexpression in haploids.

YNL330C
[RPD3] Histone deacetylase; regulates transcription and silencing histone deacetylase Null mutant is viable and shows reduced potassium dependency, mating defects, hypersensitivity to cycloheximide, and constitutive derepression of acid phosphatase; mutant epistasis analysis indicates that RPD3 acts in the same pathway as UME4/SIN3; homozygous mutant diploid is defective in sporulation and recombination YPL139C [UME1] Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p transcriptional modulator Null mutant is viable, expression of the meiotic gene IME2 in null haploid [GNP1] High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids high affinity glutamine permease Null mutant is viable but shows reduced glutamine transport and is therefore resistant to the glutamine analog L-glutamic acid gamma-monohydroxamate; overexpression induces sensitivity to heat shock YLR373C [VID22] Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Null mutant is viable but exhibits vacuole degradation of cytosolic proteins [INO2] Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion helix-loop-helix protein The null mutant is viable but auxotrophic for inositol and choline. The null mutant can also display aberant cell shape and defects in nuclear segregation. Homozygous mutant ino2 delta-1 diploids fail to sporulate. Other mutant alleles show pleiotropic defects in phospholipid metabolism.

YHL020C
[OPI1] Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance The null mutant is viable but constitutively accumulates INO1 mRNA.

YOL108C
[INO4] Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain basic helix-loop-helix (bHLH) protein The null mutant is viable but auxotrophic for inositol and choline. The null mutant expresses repressed levels of inositol-1phosphate synthase (INO1) mRNA and exhibits reduced phosphatidylcholine biosynthesis.

YOL067C
[RTG1] Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus transcription factor Null mutant is viable but cannot grow on acetate as the sole carbon source, is a glutamate and aspartate auxotroph, and shows decreased citrate synthase, acetyl-CoA synthetase, NAD isocitrate dehydrogenase, and pyruvate carboxylase activities