Fig. 2

DNA methylation divergence between cell types and cattle lineages. CpG sites were restricted to those covered by at least 10 reads in all samples (9084 sites). A, B Annotation of CpG sites overlapping defined genomic features. Where CpG sites overlapped multiple genomic features, precedence was given as follows: promoter > exon > intron. C PCA of percentage methylation of CpG sites. D Unsupervised hierarchical clustering of samples based on their percentage methylation at CpG sites. Samples are clustered based on the Spearman’s rank correlation distance between them for the top 50% of CpG sites displaying the highest standard deviation across all samples (4542 sites). gdT cell denotes γδ T cell