Fig. 1

a Shows the procedure used by SPUMONI 2 to digest a reference into a smaller reference of concatenated minimizers. In practice, SPUMONI 2 would also include the reverse complement of each sequence prior to applying the minimizer scheme. b After generating this minimizer digest which is typically smaller than the original reference, SPUMONI 2 builds an r-index over the minimizer digest which in turns leads to a smaller index