Fig. 5

Results obtained for data from the human prostate (section 3.1). T helpers―CD4⁺ T cells (helper T cells), CD4⁺ Tem―CD4⁺ effector memory T cells, Cytotox―cytotoxic CD8⁺ T cells, DCs―dendritic cells, M1―M1 macrophages, M2―M2 macrophages, Mono―monocytes, Neutro―neutrophils, NK―natural killer cells, Tregs―natural CD4⁺ regulatory T cells, Endo―endothelial cells, Epi―epithelial cells, Fibro―fibroblasts, DT―dummy type. A Heatmaps represent spatial composition of cell types across spots. Dark violet indicates the absence of the cell type in question, yellow signalizes moderate occurrence, and magenta dominance of a given type. B The inflamed region of interest annotated on the H&E image (yellow selection). C Results of CellAssign performance. Colors correspond to cell types. Other―cell types that were indicated by CellAssign in not more than 4 spots, namely B cells, dendritic cells, epithelial, M2 macrophages, CD4⁺ effector memory T cells, natural CD4⁺ regulatory T cells, NK cells, and neutrophils. D Moran's I coefficient computed for cell types indicated both by CellAssign and Celloscope. E Moran's I coefficient computed for types indicated only by Celloscope. F The correlation matrix heatmap represents the values of the Pearson correlation coefficient for all studied cell types, the positive values in red, negative in blue. 0 indicates that there is no relationship between studied variables. “X” denotes an insignificant correlation (p-values of the test with the test statistics based on the Pearson’s product moment correlation coefficient \(p \le 0.05\)). B cells and neutrophils were disregarded as those two cell types indicated only insignificant results