Fig. 2

Identification of candidate genes associated with Verticillium wilt resistance on introgressed fragments (SHZDI1/SHZDP2/AYDP1). a Manhattan plot for AYDP1 on chromosome A10 and haplotype blocks (A10:112.511–113.995 Mb) around SHZDI1/SHZDP2/AYDP1 (A10:112.70–113.75 Mb) was estimated using pairwise LD correlations (R²). b Collinearity analysis of A10 of G. hirsutum and Chr10 of G. arboreum is shown in mauve. SHZDI1/SHZDP2/AYDP1 (A10:112.70–113.75 Mb) in G. hirsutum corresponded to 132.45 to 133.60 Mb on Chr10 of G. arboreum. Corresponding homology to SHZDI1/SHZDP2/AYDP1 is marked on the purple line. c Expression patterns of 4 of 14 candidate genes at 0, 3, 9, 24, 48, and 72 h post-inoculation (hpi) with V. dahliae strain Vd080 in introgressed Hap. B (ZZ2), measured by qRT-PCR. d–f Silencing of candidate genes in cotton seedlings inoculated with Vd080. Plant wilt phenotype and stem browning symptoms were photographed at 3 weeks post-inoculation. Percentages were counted for each disease grade (increasing disease severity from grade 0 to 4). DI was the mean value of three independent experiments and was measured at 21 days post-inoculation (dpi). TRV::00 was used as a negative control, and one-way ANOVA was used in the statistical analysis, and means labeled with different letters indicate significant difference at α = 0.05. Error bars represent the SD of three biological replicates