Fig. 1
Genome-wide association using SNPs and introgression analysis on SHZDI1/SHZDP2/AYDP1. a Manhattan plot for GWAS on the Verticillium wilt for disease percentage (DP) and disease index (DI) in samples from Shihezi and Anyang. High-confidence QTLs and previously reported QTLs are marked in black and red words, respectively. b Fst analysis of A10 between the tolerant group and susceptible group based on lead SNP of SHZDI1. c SNP schematic around SHZDIP1 showing 2 haplotypes (Hap. 1 and Hap. 2) in G. arboreum and 5 haplotypes (Hap. A to Hap. E) in G. hirsutum. Number of materials is marked on the left. Hap.B of G. hirsutum carrying the introgressed G. arboreum fragments of Hap.1. Blue box indicates an introgressed region. d DI of haplotypes in greenhouse-grown G. arboreum and G. hirsutum. Significant difference was measured between different groups (Student’s t test). G. hirsutum carrying the introgressed fragments showed significantly enhanced VW resistance compared to materials without these fragments. G. arboreum variants carrying either Hap. 1 or Hap. 2 were more tolerant than G. hirsutum. e SNP-based phylogenetic tree showing that Hap.B lines of G. hirsutum harbor the introgression from A10 of G. arboreum