Fig. 1
From: Modeling group heteroscedasticity in single-cell RNA-seq pseudo-bulk data
Group variation in scRNA-seq pseudo-bulk datasets. Group variation in 4 publicly available scRNA-seq datasets with various experimental designs, with replicates samples from a mouse lungs, b Xenopus tails, c human PBMCs, and d human lungs, is summarized. For each scRNA-seq dataset, cells of one particular type were selected (see the “Methods” section), and the cells from each sample were aggregated to create pseudo-bulk counts. Multidimensional scaling plots of pseudo-bulk data were plotted in the left panel, with distances computed from the log-CPM values and samples colored by groups. Group-wise common BCVs are plotted in the middle panel. Group-wise mean-variance trends are plotted in the right panel. Colors denote groups