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Fig. 7 | Genome Biology

Fig. 7

From: m6A readers ECT2/ECT3/ECT4 enhance mRNA stability through direct recruitment of the poly(A) binding proteins in Arabidopsis

Fig. 7

CPSF30-L and ECT2/ECT3/ECT4 bound some of the same m6A sites and execute distinct RNA fate regulation. a Overlap of the identified CPSF30-L & m6A targets and ECT2 & m6A targets corresponding to 386 unique transcripts with the same m6A site (termed ECT2/CPSF30-L & m6A common targets). b Bar plots showing the amount of PAC shifted and Non-PAC shifted genes in ECT2/CPSF30-L & m6A common targets in ect2/3/4 and cpsf30-l mutants, respectively. c Bar plots showing the amount of upregulated and downregulated genes in ECT2/CPSF30-L & m6A common targets in ect2/3/4 and cpsf30-l mutants, respectively. d Integrative genomics viewer showing sequencing results for AT4G39080 transcripts. The light blue box at the far right of each line indicates the position of the m.6A site, ECT2 and CPSF30-L binding sites, and the position of the shifted poly(A) site. PA1 and PA2 are indicated as proximal and distal poly(A) sites. The y-axis scales were normalized by the mapped sequencing reads. e Relative expression of proximal and distal transcripts produced by PA1 and PA2 in WT, ect2/3/4, and cpsf30-l plants. f Relative mRNA level of AT4G39080 in WT, ect2/3/4, and cpsf30-l plants. TUB8 was used as the internal control gene. Data are presented as the mean ± SE; n = 2 biological replicates × 2 technical replicates. *P < 0.05, **P < 0.01 (two-sided t-test)

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