Fig. 2
From: Cross-phyla protein annotation by structural prediction and alignment
Signaling pathways in Spongilla pinacocytes. A Dotplot of pinacocyte signaling and effector genes. Cell types of the pinacocyte family are encased by a red square. Genes on blue background were annotated by Musser et al. with sequence-based methods (“legacy annotation”). Genes on yellow background are annotated by MorF. Showing mean expression of log-transformed, normalized counts. B Superposition of Spongilla FGF (blue, 61–230 aa) and Gallus gallus FGF4 (UniProtID: P48804) (yellow, 54–194 aa) (RMSD = 0.89 over 543 atoms). C Superposition of Spongilla ephrin (blue, 1–153 aa) and C. elegans efn-3 (UniProtID: Q19475) (yellow, 29–179 aa) (RMSD = 1.59 over 580 atoms). In both cases, the structural similarity is apparent despite their low sequence identity of 11.8% and 22% respectively. Superpositions were created using the super command in PyMOL (v2.3.5). Cell type abbreviations: incPin, incurrent pinacocytes; apnPn, apendopinacocytes; lph, lophocytes; basPin, basopinacocytes; scp, sclerophorocytes; met, metabolocytes; chb, choanoblasts; cho, choanocytes; apo, apopylar cells; myp, myopeptidocytes; amb, amoebocytes; grl, granulocytes; nrd, neuroid cells; mes, mesocytes; arc, archaeocytes; scl, sclerocytes