Fig. 7

Gut microbiota shapes the hepatic HNF4α binding followed by altered Cyp8b1 responsible for bile acid metabolism. A 23 identified differential bile acids (VIP > 0, p-value < 1) between HFD and HFDR groups (n = 9–10 per group). B 9 identified differential bile acids (VIP > 1, p-value < 1) between HFD and HFDR groups (n = 9–10 per group). C Heatmap of 9 identified differential bile acids (VIP > 1, p-value < 1) between HFD and HFDR groups (n = 9–10 per group). D–L Relative concentrations of differential representative bile acids between HFD and HFDR groups (n = 9–10 per group). M Western blot analysis of HNF4α in liver from HFD and HFDR groups (n = 4 per group). N Relative mRNA expression levels of Cyp8b1 (n = 8 per group). O ChIP-qPCR analysis of Cyb8b1 binding to HNF4α (n = 3 per group). Multiple testing correction was calculated. * FDR < 0.1, ** FDR < 0.05, *** FDR < 0.01 and **** FDR < 0.001 were determined statistically significant