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Fig. 6 | Genome Biology

Fig. 6

From: Caloric restriction remodels the hepatic chromatin landscape and bile acid metabolism by modulating the gut microbiota

Fig. 6

Gut microbiota transplantation alleviates HFD-induced metabolic syndrome and shapes gut microbiota composition. A The pattern diagram of gut microbiota transplantation in HFD mice. B Cumulative weight gain of male mice after gut microbiota transplantation (n = 8 per group). C Cumulative food intake of male mice after gut microbiota transplantation (n = 9–10 per group). D H&E staining of adipose tissue and liver in HFD and HFDR group (× 20 magnification; scale bar indicates 100 Î¼m). BAT: Brown adipose tissue, WAT: White adipose tissue; Oil Red O staining of liver in HFD and HFDR group (× 20 magnification; scale bar indicates 100 Î¼m). E Serum lipids levels of male mice from HFD or HFDR group (n = 6 per group). TC: total cholesterol, TG: triglyceride, LDL: low-density lipoprotein, HDL: high-density lipoprotein (n = 6 per group). F Serum leptin levels of male mice from HFD or HFDR group (n = 6 per group). G Serum ALT, AST, and ALP levels of male mice from HFD or HFDR group (n = 6 per group). H,I IPGTT tests in male mice from HFD and HFDR groups at 14 weeks and quantification of the AUC (n = 6 per group). J Plasma glucose levels of male mice in HFD and HFDR groups (n = 6 per group). K,L IPITT tests in mice from HFD and HFDR groups at 14 week and quantification of the AUC (n = 6 per group). M,N PCoA of the Jaccard and unweighted_unifrac distances at 15-week in HFD and HFDR group (n = 9–10 per group). O,P Phylum-level proportional abundance of cecal gut microbiota in donors (ALD and CRD) and recipients (HFD and HFDR) mice at 15 weeks (n = 9–10 per group). Each color represents a phyla. Q 20 OTUs associated with the differentiation of the gut microbiota between the HFD and HFDR groups based on random forests analysis (n = 9–10 per group). Significance was calculated using non-paired two-tailed Student’s t test and permutational multivariate analysis of variance test (beta diversity). ∗ p < 0.05, ∗  ∗ p < 0.01, ∗  ∗  ∗ p < 0.001, ∗  ∗  ∗  ∗ p < 0.0001

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