Fig. 5

Short-term caloric restriction alters HNF4α distribution in liver. A Western blot analysis and quantitation of PPARα and HNF4α protein expression levels in AL and CR livers (n = 5 per group). B Heatmap of HNF4α, H3K27ac, and ATAC differentially CR gain or loss regions in AL and CR groups (n = 2 per group). C ChIP signals of HNF4α, H3K27ac, and ATAC in gain or loss region between AL and CR groups (n = 2 per group). D ChIP-seq of HNF4α and H3K27ac at the Fom3 locus as a representative example (n = 2 per group). E ChIP-seq of HNF4α and H3K27ac at the Cyp7b1 locus as a representative example (n = 2 per group). F ChIP-seq of HNF4α and H3K27ac at the Cyp8b1 locus as a representative example (n = 2 per group). G,H GO pathway analysis of loci with differential HNF4α binding sites. I Venn diagram of DEGs (both up and down) induced by AL or CR and differential HNF4α peaks associated genes (n = 2 per group). H Expression levels of DEGs with CR-gained HNF4α peaks in AL and CR groups (n = 2 per group). Significance was calculated using non-paired two-tailed Student’s t test. ∗ p < 0.05, ∗  ∗ p < 0.01, ∗  ∗  ∗ p < 0.001, ∗  ∗  ∗  ∗ p < 0.0001