Fig. 4
From: Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants
Robust genome editing by LbCas12a-RRV in rice T0 transgenic plants. A Diagram of multiplexing four crRNAs to simultaneously target eight sites using a dual ZmUbi promoter and tandem HH-crRNA-HDV system. B Indel frequencies of eight target sites in rice T0 plants with each dot representing one T0 plant. C Genotypes of T0 plants at GA1-TTTA, Os11g19880-TTC and Os12g12600-TTA sites. Wild type (WT) denoted as empty rectangles, biallelic edit denoted as fully filled rectangles, monoallelic edit denoted as half-filled rectangles, chimeric edit denoted as doted rectangles. One-way analysis of variance (ANOVA) with Tukey’s multiple comparison test (p < 0.05) were analyzed using GraphPad Prism 9. Bars without assigned letters indicate no significant differences among four LbCas12a nucleases