Fig. 3
From: Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants
Enhanced editing efficiency of LbCas12a-RV in rice and tomato protoplasts using plasmid and RNP delivery. A Diagram of the plasmid expression system of Cas12a and crRNA. B Editing efficiency of LbCas12a variants at four TTTV target sites and four VTTV target sites in rice protoplasts. C Diagram of RNP delivery in rice protoplasts. Rice protoplasts were transfected with 0.01 μM RNP and incubated in two temperature conditions (25 °C and 32 °C) for 2 days. D Editing efficiency of LbCas12a and LbCas12a-RV at two TTTV target sites and two VTTV target sites in rice protoplasts with RNP delivery. E Diagram of RNP delivery in tomato protoplasts for multiplexed genome editing. Tomato protoplasts were transfected with 0.06 μM RNP (0.01 μM RNP per target) and incubated in two temperature conditions (25 °C and 32 °C). F Editing efficiency of LbCas12a and LbCas12a-RV at six target sites in tomato protoplasts with RNP delivery. The editing efficiency was measured by NGS. One-way analysis of variance (ANOVA) with Tukey’s multiple comparison test (p < 0.05) were analyzed using GraphPad Prism 9