Fig. 2
From: Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants
LbCas12a-RVQ is a novel variant with robust editing efficiency in human cells. A The normalized editing efficiencies of 56 LbCas12a variants in human HEK293 cells over four target sites when delivered as RNP. See Additional file 1: Fig. S2A for the raw editing efficiency. B Mapping the validated positive hits with enhanced activity on the existing LbCas12a structure. Red: residues used in LbCas12a-RVQ constructs. Gold: other residues with improved activity but not included in the LbCas12a-RVQ. C Editing efficiency of LbCas12a-WT, LbCas12a-RVQ and LbCas12a-RRVQ in HEK293 cells over additional 11 target sites at a low RNP dosage (0.1 μM). NC indicates negative control. One-way analysis of variance (ANOVA) with Tukey’s multiple comparison test (p < 0.05) were analyzed using GraphPad Prism 9. D, E The DNA binding specificities of WT LbCas12a (D) and LbCas12a-RVQ (E) measured by Spec-seq over the same target site and presented as motif logo for visualization. F, G The DNA cleavage specificities of WT LbCas12a (F) and LbCas12a-RVQ (G) measured by SEAM-seq