Fig. 5

Cell-type-specific regulatory networks and key regulators for root hair differentiation. a Mapping the information of chromatin openness revealed by snATAC-seq data into the cell clusters classified by snRNA-seq. b UMAP visualization of 10 cell clusters were annotated by both snRNA-seq and snATAC-seq for wheat root. Each dot represents a single cell. c High correlation between chromatin accessibility and expression of marker genes for each corresponding clusters. Left part is the open chromatin scores calculated based on snATAC-seq, Right part is the expression level calculated based on snRNA-seq. d Top5 representative TF regulons for each cluster identified by SCENIC4. The abbreviated names of TF regulons were followed with the chromosome name to indicate their location in subgenomes. e Root transections of taspl14 knock-out line and wild type. The protoxylem pores and companion cells were marked with yellow arrow and pink arrow head, respectively. Bar is 100 μm. f taspl14 knock-out line showed reduced companion cells and increased protoxylem. g BAM1 and LOB were downregulated in root of taspl14 knock-out lines. h The accessible chromatin regions (ACRs) of BAM1 homolog (TraesCS4D02G235800). i Differentiated trajectories of root hair. Colors of dots are corresponding to cell clusters. Start indicates the initiation of the pseudo-time trajectory. Terminal indicates the end of the pseudo-time trajectory. j Trajectory network for root hair differentiation identified the key regulators for cell identity transition