Fig. 5

The long-distance H3K27me3 correlation between cis-regulators and promoters. A, B We selected two genomic regions (chr20:31,866,019 ~ 31,871,019 gene Dsup15, chr20:44,650,712 ~ 44,655,712 gene Ada), which included the documented promoters and enhancers. Each pixel in the heatmap means the 50 bp length. The correlation index calculated based on method. The higher correlation index (red) between two regions suggested the accordant changes. We could observe the high correlation (black square) between the enhancers and promoters. The lower black curves indicated the interaction signals in Hi-C data. The physically interacted regions (Hi-C) also supported the high H3K27me3 correlation. C We further analyzed the promoter upstream 2-kb regions to see whether there were some strongly correlated genomic areas, the potential cis-regulators for the promoters. The calculation details could be found on the method. Each pixel in the heatmap was 50 bp length. Some prompters strongly correlated with their proximal regions (black box), suggesting that these proximal regions synchronized with the corresponding promoters in the H3K27me3 regulation. D, E By further analyzing the binding motif of these proximal regions, we found these regions have the binding motifs for the transcription regulators, modulating the transcription activity