Fig. 7From: Large-scale analysis of de novo mutations identifies risk genes for female infertility characterized by oocyte and early embryo defectsEffects of mutations on polar body extrusion and blastocyst formation in mice. a The effects of the mutations on polar body (PB) extrusion. The above shows the diagram of GV oocytes collection, microinjection, and polar body extrusion assay. The below shows the statistical analysis of polar body extrusion rate of wild-type and mutations. Significance was compared between wild-type and mutant groups. Two independent experiments were performed and followed Fisher’s exact test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. b The effects of the mutations on blastocyst formation. The above shows the diagram of zygote collection, microinjection, and blastocyst formation assay. The below shows the statistical analysis of blastocyst rate of wild-type and mutations. Two independent experiments were performed and followed Fisher’s exact test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significantBack to article page