Fig. 7
From: Multi-omics analysis identifies drivers of protein phosphorylation
Genetic regulation of PCCA and PCCB across three tissues. A Co-mapping distant phQTL of PCCA pS248 was identified in liver and heart but not kidney tissue. NZO allele drove the low level of this phosphorylation event. Data were categorized based on the founder haplotye at the identified phQTL. B Abundances of PDK1 and PCCA pS248 were highly correlated in heart and liver but not in kidney tissue. Abundance of PDK1 and PCCA pS248 in each individual sample (116) were categorized based on the haplotye of the phQTL on PCCA pS248 in heart and liver tissues on Chromosome 2. C Genome scans for PCCA pS248 are overlayed with mediation scores in heart and liver tissues. Each gray dot is a mediation score representing the PCCA pS248 phQTL LOD score conditioned on a protein as candidate mediator. D Genome scans of PCCA and PCCB in all the three tissues. Local pQTL for PCCB and distant pQTL for PCCA co-mapped to the same locus in heart tissue. PCCB was identified as the mediator of the PCCA distant pQTL. Local pQTL for PCCA and distant pQTL for PCCB co-mapped to the same locus in liver tissue and kidney tissues. PCCA was identified as the mediator of the PCCB distant pQTL. E Allele effects of identified pQTL for PCCA and PCCB in the three tissues. F Protein abundance of PCCA and PCCB were highly correlated in each tissue. Protein abundance in each individual sample (116) were categorized based on the haplotye of the pQTL on PCCA in kidney and liver tissues on Chromosome 14. G The transcript level of Pccb is distinctly higher than the mRNA level of Pcca in kidney and liver tissues but not in heart tissue. mRNA abundance of Pcca and Pccb in each individual sample (116) were categorized based on the haplotye of the pQTL on PCCA in heart tissue on Chromosome 9. H Sex-interactive local pQTL on PCCA and sex-interactive distant pQTL on PCCB co-mapped to the same locus in the kidney tissue, characterized by a distinct NZO effect (NZO males with greater abundances than NZO females). PCCA was identified as the mediator of PCCB sex-interactive distant pQTL. Points are colored by founder haplotype at sex-interactive phQTL. Males and females from the same CC strain were connected by a line