Fig. 6

Phosphorylation sites on one protein can be regulated coordinated and not coordinated. A Heatmap of Pearson correlations of abundances of phosphopeptides from parent protein ABLIM1. B Genome scans of pS539 and pS56 on UCKL1 in kidney tissue. C A local CAST allele at Uckl1 drove low abundance of UCKL1 pS539 in kidney tissue. D Distant NOD and PWK allele on chromosome 18 drove low abundance of UCKL1 pS56 in kidney tissue. E Heatmap of Pearson correlations among all proteins quantified in ATP synthase complex in heart tissue. F The AJ allele at Atp5h drove low abundance of the entire ATP synthase complex in heart tissue. All quantified ATP synthase complex subunits have low protein abundance in CC032, CC033, and CC044 strains, which possess the AJ allele, in heart data. G Mediation analysis using proteomics data identified ATP5H as the mediator of a phQTL for ATP5E. Each gray dot is a mediation score representing the ATP5E pQTL LOD score conditioned on a protein as candidate mediator. ATP5H was detected as the strongest mediator of the ATP5E distal pQTL in heart tissue. All ATP synthase complex subunits have mediation z-scores < −8 and were highlighted in black. Other quantified ATP synthase complex subunits, ATP5S, ATP5G2, and ATP5J, were highlighted in blue. Horizontal dashed line at LOD of 6 was included for reference. H Heatmap of Pearson correlations among all phosphorylation events quantified from the ATP synthase complex in heart tissue. The correlations among the five sites from ATP5A1 are highlighted by a dashed square. Correlation with FDR < 0.01 were highlighted using stars. I Genome scans of ATP5A1 pS53 in the three tissues, revealing co-mapping phQTL in all the three tissues. J Allele effects of ATP5A1 pS53 phQTL were highly correlated in the three tissues