Fig. 4

Enhancer activation can occur independently of MLL3/4. A Pie charts showing fraction of coincident changes in H3K27ac within the different categories of changing H3K4me1. B Heatmap of sites that normally lose H3K4me1 and H3K27ac during naive to formative transition clustered by either MLL3/4-dependent or MLL3/4-independent H3K27ac. Rows sorted on “S” columns. Y-axis scale of metagene profiles above is same as signal ranges of heatmap, values in CPM. C Same as B but using sites that normally gain H3K4me1 and H3K27ac during naive to formative transition clustered by either MLL3/4-dependent or MLL3/4-independent H3K27ac. D Same as B but using sites that have unchanging H3K4me1 but gain H3K27ac during naive to formative transition clustered by either MLL3/4-dependent or MLL3/4-independent H3K27ac. All heatmap values and range are in CPM. For metagene analysis, the range in CPM is the same as shown in heatmap for each factor. E Naive and formative examples of functionally validated enhancers that are either MLL3/4 dependent or independent. Naive MLL3/4 dependent Sox2 enhancer cluster, Naive MLL3/4 independent Nanog enhancers, Formative independent and dependent enhancers of Fgf5, formative independent enhancer of Pou3f1/Oct6