Fig. 3

MLL3/4-dependent and MLL3/4-independent distal H3K27ac deposition. A Differential signal enrichment between naive and formative WT H3K27ac peaks identifies significant peaks (Black, FDR <0.05, Log2 Foldchange (Log2FC) > 1). B Scatterplot of H3K27ac peak intensities between naive and formative WT samples (gray, shared peaks, FDR > 0.1 & Log2FC < 0.7, red or blue, FDR < 0.05 & Log2FC > 1). C H3K27ac signal in naive and formative DKO samples at peaks called from WT. Colors correspond to peak categories derived from WT. D Feature annotation of WT or DKO peaks stratified by peak category including intergenic/intronic (Distal), Promoter, and all else (Others). E Distal H3K27ac peak categories further stratified by MLL3/4 independent (DKO/WT Log2FC > −0.7) or dependent (DKO/WT Log2FC < −1). G,H Heatmap of MLL3/4-independent and MLL3/4-dependent H3K27ac peak categories for naive or formative-specific peaks, rows sorted on “S” columns. All heatmap values and range are in CPM. For metagene analysis, the range in CPM is the same as shown in heatmap for each factor. I nearest neighbor TSS analysis of expression levels Log2CPM near H3K27ac peak categories. Multi-comparison paired Wilcoxon rank-sum test, Benjamini-Hochberg corrected. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001