Fig. 1

MLL3/4 is dispensable for transcriptional activation of much of the formative program. A Schematic of naive to formative transition in vivo and in vitro. B Western validation of MLL3 and MLL4 knockout cell lines. C Brightfield microscopy of cell lines in naive and formative conditions, 20×, scale bar = 100μm. D Differential gene expression analysis (DGE) on WT naive and formative RNA-seq samples (significant genes colored, P.adj < 0.05, Log2 Foldchange (Log2FC) >1). E UMAP analysis on all RNA-seq samples subset by all naive and formative genes from WT DGE. F,G Fold change (formative/naive) of all genes for either MLL3KO and DKO compared to WT. WT formative and naive genes from WT DGE analysis colored. R, Pearson’s coefficient. Dashed line and linear equation represent linear model of all genes. H DGE analysis on WT naive and DKO naive samples (significant genes colored, P.adj < 0.05 and Log2FC > 1). I Naive state DKO misexpressed genes categorized by whether they are also WT naive or formative genes. J,K Same as H,I respectively but comparing within the formative state