Fig. 4

Mutational scanning shows the structural logic of trafficking in Kir2.1. Heatmap of surface expression fitness scores calculated from Enrich2 gradient colored from red (less than WT fitness) to white (WT fitness) to red (greater than WT fitness). Cartoon of secondary structure and labels of structural elements denoted above the heatmap. Only positions for which there were reads in all three replicates are shown here; others were removed in enrichment calculations. Synonymous mutation boxes are outlined with green. Mutations without data are highlighted in light yellow