Fig. 2

Quantifying the bias of library assembly with DIMPLE. A Boxplots of variants at each position across Kir2.1. The vertical length of the box is the interquartile range (IQR), upper bound is the 75th percentile with the lower bound is the 25th percentile. Significance is tested using two-sided t-tests controlled for multiple comparisons comparing incorporation means between variants across all positions. Significance levels: ***P < 0.001; **P < 0.01; *P < 0.05, all others not significant. B Stacked density plots, or ridge plot ordered bottom-to-top from first to last positions of the second sublibrary of Kir2.1. C Lorentz curves and Gini coefficients test the inequality within the distribution of observed variants. A completely even distribution would be a diagonal with a Gini score of 0. The distribution of designed variants for mutagenic libraries of Kir2.1, TRPV1, VatD, and OPRM1 are shown with corresponding Gini scores noted