Fig. 1From: DIMPLE: deep insertion, deletion, and missense mutation libraries for exploring protein variation in evolution, disease, and biologyGeneration of programmed mutational, deletional, and insertional libraries with DIMPLE in the model potassium channel Kir2.1. A Schematic depiction of the library generation process with DIMPLE. B Bar plot of mutation type per position against counts. All variants are stacked. Dashed lines indicate the boundaries of each mutagenic sublibrary. Overall, each mutagenized sublibrary region is within 2-fold of each other, indicating well-balanced libraries (Additional file 3: Fig S3B)Back to article page