Fig. 1
An overview of the plant epitranscriptome and techniques for mapping epitranscriptome. a Known RNA modification in plants. Predominant locations of various RNA modifications are illustrated in a transcript. The mRNAs are capped with N7-methylguanosine (m7G) or nicotinamide adenine diphosphate (NAD+) and contain internal modifications, including N6-methyladenosine (m6A), N1-methyladenosine (m1A), 5-methylcytosine (m5C), and pseudouridine (ψ), in plants. b Approaches for mapping RNA modifications. Techniques coupling next generation sequencing with antibody-, chemical-, and enzyme-based approaches are shown in the upper panels, while nanopore direct RNA sequencing is illustrated in the lower panel. c Comparison of the key features of different m6A profiling techniques. The asterisk indicates the time required for sample preparation before library construction and sequencing. Created with Biorender.com