Fig. 6

Not4 cross-linking correlates with changes in mRNA solubilities upon Not4 depletion and Not1 promoter association correlates with mRNAs less soluble upon Not1 depletion. A Box plot comparing Not1 RIP in cells lacking Not5 for the categories of mRNAs color-coded in Fig. 2C. Significance of differences is indicated at the top of the box plots—p-values are calculated using a two-sided Welch two-sample t-test. B Comparative cross-linking (normalized density) of Not4 (left) and RNAPII (right) to different mRNA sequences as indicated. C Same as panel A, but comparing the relative Not4/RNAPII cross-linking to the different classes of mRNAs. D Heat map comparing signal of free MNAse and Not1-MNase DNA cleavage events at promoter regions (ChEC). E Heat map comparing Not1-ChEC signal and changes in mRNA solubilities upon Not1 and Not4 depletion. F Box plot analysis of the Not1-ChEC signal in the different promoters of the genes encoding the different classes of mRNAs color-coded in Fig. 2C. Significance of differences is indicated at the top of the box plots—p-values are calculated using a two-sided Welch two-sample t-test