Fig. 4

Species-specific H3K27me3 in oocytes is associated with species-specific non-canonical imprinting Zfp64 and Zfp516, and transient imprinting of Bbx. a 2D scatterplot showing genome-wide H3K36me3 and H3K27me3 levels over 1-kb bins in rat oocytes. Datapoints are colored by average oocyte DNAme levels. A random subset of 10,000 bins is shown. The density of all data points (n=1,164,268) is summarized by a contour plot. b Cross-species Spearman correlation values between H3K4me3, H3K27me3, and H3K36me3, and DNAme levels over syntenic 1-kb bins in rat and mouse oocytes (n=823,926). c Scatterplot showing differences in rat and mouse oocyte DNAme, H3K27me3, and H3K36me3 levels over syntenic 1-kb bins. A random set of 10,000 bins are shown, and all bins (n=823,926) are summarized by a contour plot. The number of bins with a delta H3K27me3 >0.5 RPKM and DNAme >75% is indicated by a dashed box. d–f Rat and mouse genome browser screenshots of the Zfp64-LOC108350526, Zfp516, and Bbx loci. Replicates of CUT&RUN data were merged and mean levels are shown as counts per million aligned reads (CPM). Promoters are indicated by a dashed line